BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0585.p (122 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309955|ref|YP_003774047.1| two-component system sensor his... 240 3e-65 gi|300312287|ref|YP_003776379.1| chemotaxis cheY protein [Herbas... 124 1e-30 gi|300311034|ref|YP_003775126.1| chemotaxis response regulator p... 103 6e-24 gi|300311785|ref|YP_003775877.1| chemotaxis response regulator C... 100 6e-23 gi|300311339|ref|YP_003775431.1| CheY chemotaxis protein [Herbas... 89 1e-19 >gi|300309955|ref|YP_003774047.1| two-component system sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 122 Score = 240 bits (612), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 115/122 (94%), Positives = 119/122 (97%) Query: 1 MEKSILIVDDSFSLRQTISAALKSAGYHVEEATDGRQALSKLDGRKFNLVISDLNMPHLD 60 MEK+ILIVDDSFSLRQTISAALKSAGYHVEEA DG+QAL+ LDGRKFNLVISDLNMPHLD Sbjct: 1 MEKNILIVDDSFSLRQTISAALKSAGYHVEEAADGKQALAHLDGRKFNLVISDLNMPHLD 60 Query: 61 GLSFVREMKQMPAYKFTPVIMLTTESSEARKQEGKAAGIRAWVHKPFQPPVLLDAVAKLV 120 GLSFVREMKQMPAYKFTPVIMLTTESSEARKQEGKAAGIRAWVHKPFQPPVLLDAVAKL+ Sbjct: 61 GLSFVREMKQMPAYKFTPVIMLTTESSEARKQEGKAAGIRAWVHKPFQPPVLLDAVAKLI 120 Query: 121 QP 122 P Sbjct: 121 LP 122 >gi|300312287|ref|YP_003776379.1| chemotaxis cheY protein [Herbaspirillum seropedicae SmR1] Length = 121 Score = 124 bits (312), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 58/120 (48%), Positives = 83/120 (69%) Query: 1 MEKSILIVDDSFSLRQTISAALKSAGYHVEEATDGRQALSKLDGRKFNLVISDLNMPHLD 60 M K+IL VDDS SLRQ + +LK+AGY+V EA DG AL K + F+LV++D NMP +D Sbjct: 1 MSKTILAVDDSGSLRQMVVFSLKAAGYNVTEAVDGVDALEKAKSQTFDLVLTDQNMPRMD 60 Query: 61 GLSFVREMKQMPAYKFTPVIMLTTESSEARKQEGKAAGIRAWVHKPFQPPVLLDAVAKLV 120 GL+ +R ++++ +Y P++MLTTE S+ K +GKAAG W+ KPF P L + V K++ Sbjct: 61 GLTLIRSLRELTSYARVPILMLTTEFSDEMKAKGKAAGANGWLVKPFDPQRLTEVVRKVI 120 >gi|300311034|ref|YP_003775126.1| chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 126 Score = 103 bits (256), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 48/119 (40%), Positives = 79/119 (66%), Gaps = 1/119 (0%) Query: 3 KSILIVDDSFSLRQTISAALKSAGYHVEEATDGRQALSKLD-GRKFNLVISDLNMPHLDG 61 KSI +VDDS ++ ++ + L+ AG+ V+ A DG +AL K+ G K +L+I+D+NMP++DG Sbjct: 5 KSIFLVDDSATMLMSVRSHLEMAGFAVDTAEDGVKALKKMQAGYKPDLLITDINMPNMDG 64 Query: 62 LSFVREMKQMPAYKFTPVIMLTTESSEARKQEGKAAGIRAWVHKPFQPPVLLDAVAKLV 120 L +R ++ +P ++F P++ LTTES ++ EGK AG W+ KP LL V +++ Sbjct: 65 LELIRNVRALPGFRFIPILTLTTESQSGKRDEGKKAGATGWLVKPVGGADLLKIVRQVL 123 >gi|300311785|ref|YP_003775877.1| chemotaxis response regulator CheY [Herbaspirillum seropedicae SmR1] Length = 122 Score = 99.8 bits (247), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 52/117 (44%), Positives = 71/117 (60%), Gaps = 1/117 (0%) Query: 5 ILIVDDSFSLRQTISAALKSAGYHVEEATDGRQALSKLDGR-KFNLVISDLNMPHLDGLS 63 IL+VDDS ++R + LK G V A DGR LSKL G LV+SD+NMP++DGL+ Sbjct: 4 ILVVDDSSTVRAEVGDFLKKNGLDVALAVDGRDGLSKLKGDPAIKLVVSDVNMPNMDGLT 63 Query: 64 FVREMKQMPAYKFTPVIMLTTESSEARKQEGKAAGIRAWVHKPFQPPVLLDAVAKLV 120 +++ +IMLTTE+S K+ GKAAGI+ W+ KPF+ +L KLV Sbjct: 64 MAEKIRSELGNASVNIIMLTTENSPIMKERGKAAGIKGWIVKPFKGEAVLPTFKKLV 120 >gi|300311339|ref|YP_003775431.1| CheY chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 131 Score = 89.0 bits (219), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 45/118 (38%), Positives = 72/118 (61%), Gaps = 1/118 (0%) Query: 5 ILIVDDSFSLRQTISAALKSAGY-HVEEATDGRQALSKLDGRKFNLVISDLNMPHLDGLS 63 L+VDD ++R+ + LK GY +V+EA DG QAL KL +F+ V+SD NMP++DGL+ Sbjct: 8 FLVVDDFSTMRRIVRNLLKELGYTNVDEAEDGVQALQKLRSDQFDFVVSDWNMPNMDGLT 67 Query: 64 FVREMKQMPAYKFTPVIMLTTESSEARKQEGKAAGIRAWVHKPFQPPVLLDAVAKLVQ 121 ++E+++ PA PV+M+T E+ + AG +V KPF L + + K+ + Sbjct: 68 MLQEIRKDPALSKLPVLMVTAEAKKENIVAAAQAGANGYVVKPFTAATLDEKLGKIFE 125 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 342,117 Number of extensions: 11745 Number of successful extensions: 146 Number of sequences better than 1.0e-08: 31 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 31 Length of query: 122 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 48 Effective length of database: 1,269,480 Effective search space: 60935040 Effective search space used: 60935040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)