BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0578.p (646 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309950|ref|YP_003774042.1| molecular chaperone protein [He... 1285 0.0 gi|300312439|ref|YP_003776531.1| molecular chaperone protein [He... 457 e-129 gi|300312141|ref|YP_003776233.1| molecular chaperone, Hsp70 fami... 431 e-121 >gi|300309950|ref|YP_003774042.1| molecular chaperone protein [Herbaspirillum seropedicae SmR1] Length = 646 Score = 1285 bits (3324), Expect = 0.0, Method: Compositional matrix adjust. Identities = 642/646 (99%), Positives = 645/646 (99%) Query: 1 MGKIIGIDLGTTNSCVAVMEGNQPKVIENSEGARTTPSIIAYQEDGEILVGAPAKRQAVT 60 MGKIIGIDLGTTNSCVAVMEGNQPKVIENSEGARTTPSIIAYQEDGEILVGAPAKRQAVT Sbjct: 1 MGKIIGIDLGTTNSCVAVMEGNQPKVIENSEGARTTPSIIAYQEDGEILVGAPAKRQAVT 60 Query: 61 NPKNTIYAAKRLIGRKFTEKEVQKDINLMPYQIVAADNGDAWIGVRDKKLAPPQISAEVL 120 NPKNTIYAAKRLIGRKFTEKEVQKDINLMPYQIVAADNGDAWIGVRDKKLAPPQISAEVL Sbjct: 61 NPKNTIYAAKRLIGRKFTEKEVQKDINLMPYQIVAADNGDAWIGVRDKKLAPPQISAEVL 120 Query: 121 RKMKKTAEDYLGEEVTEAVITVPAYFNDAQRQATKDAGRIAGLDVKRIINEPTAAALAFG 180 RKMKKTAEDYLGEEVTEAVITVPAYFNDAQRQATKDAGRIAGLDVKRIINEPTAAALAFG Sbjct: 121 RKMKKTAEDYLGEEVTEAVITVPAYFNDAQRQATKDAGRIAGLDVKRIINEPTAAALAFG 180 Query: 181 LDKAEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQRLIDY 240 LDKAEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQRLIDY Sbjct: 181 LDKAEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQRLIDY 240 Query: 241 IIEEFKKINGLDLSKDAIALQRIKASAERAKIELSSSQQTEINEPYIAMANGAPVHLNLK 300 II+EFKKINGLDLSKDAIALQRIKASAERAKIELSSSQQTEINEPYIAMANGAPVHLNLK Sbjct: 241 IIDEFKKINGLDLSKDAIALQRIKASAERAKIELSSSQQTEINEPYIAMANGAPVHLNLK 300 Query: 301 ITRAKLESLVEELIAKTIEPCRTAIKDAGVKVSDIDDVILVGGMTRMPKVIEKVKEFFGK 360 ITRAKLESLVEELIAKTIEPCRTAIKDAGVKVSDIDDVILVGGMTRMPKVIEKVKEFFGK Sbjct: 301 ITRAKLESLVEELIAKTIEPCRTAIKDAGVKVSDIDDVILVGGMTRMPKVIEKVKEFFGK 360 Query: 361 DPRRDVNPDEAVAVGAAIQGSVLSGDRKDVLLLDVTPLSLGIETMGGVMTKMIKKNTTIP 420 DPRRDVNPDEAVAVGAAIQGSVLSGDRKDVLLLDVTPLSLGIETMGGVMTKMIKKNTTIP Sbjct: 361 DPRRDVNPDEAVAVGAAIQGSVLSGDRKDVLLLDVTPLSLGIETMGGVMTKMIKKNTTIP 420 Query: 421 TKFSQVFSTADDNQPAVTIKVYQGEREMAAGNKALGEFNLEGIPPAPRGTPQIEVTFDID 480 TKFSQVFSTADDNQPAVTIKVYQGEREMA GNKALGEFNLEGIPPAPRGTPQIEVTFDID Sbjct: 421 TKFSQVFSTADDNQPAVTIKVYQGEREMAVGNKALGEFNLEGIPPAPRGTPQIEVTFDID 480 Query: 481 ANGILHVGAKDKATGKENKITIKANSGLNEEEIEKMVRDAEANAEEDKRLKELAESRNQG 540 ANGILHVGAKDKATGKENKITIKANSGLNEEEIEKMVRDAEANAEEDKRLKELAE+RNQG Sbjct: 481 ANGILHVGAKDKATGKENKITIKANSGLNEEEIEKMVRDAEANAEEDKRLKELAETRNQG 540 Query: 541 DALVHSTRKALGEYGDKLEAGEKDVIEAAIKNLEEALKGDDKAAIDAKTEALSTAAQKLG 600 DALVHST+KALGEYGDKLEAGEKDVIEAAIKNLEEALKGDDKAAIDAKTEALSTAAQKLG Sbjct: 541 DALVHSTKKALGEYGDKLEAGEKDVIEAAIKNLEEALKGDDKAAIDAKTEALSTAAQKLG 600 Query: 601 EKMYADQQAQAAAAGGAAGAAGAQAGAAGAAPKDDNVVDADFKEVK 646 EKMYADQQAQAAAAGGAAGAAGAQAGAAGAAPKDDNVVDADFKEVK Sbjct: 601 EKMYADQQAQAAAAGGAAGAAGAQAGAAGAAPKDDNVVDADFKEVK 646 >gi|300312439|ref|YP_003776531.1| molecular chaperone protein [Herbaspirillum seropedicae SmR1] Length = 621 Score = 457 bits (1175), Expect = e-129, Method: Compositional matrix adjust. Identities = 261/618 (42%), Positives = 389/618 (62%), Gaps = 32/618 (5%) Query: 5 IGIDLGTTNSCVAVMEGNQPKVIENSEGARTTPSIIAYQEDGEILVGAPAKRQAVTNPKN 64 +GIDLGTTNS VA + + P+V+ + +G PS++ Y +G +G A+ T+P+N Sbjct: 22 VGIDLGTTNSLVATVRNSIPEVLADDDGRVLLPSVVRYLPNGHANIGYKAQAAQTTDPRN 81 Query: 65 TIYAAKRLIGRKFTEKEVQKDINLMPYQIVAADNGDAWIGVRDKKLA----PPQISAEVL 120 TI + KR +GR K++ N MPY D D V+ K +A P ++SA++L Sbjct: 82 TIVSVKRFMGRGL--KDIAHAEN-MPY-----DFQDQPGMVQIKTVAGVKSPVEVSAQIL 133 Query: 121 RKMKKTAEDYLGEEVTEAVITVPAYFNDAQRQATKDAGRIAGLDVKRIINEPTAAALAFG 180 +++TAED LG+++ AVITVPAYF+DAQRQATKDA ++AGL+V R++NEPTAAA+A+G Sbjct: 134 ATLRQTAEDALGDDLVGAVITVPAYFDDAQRQATKDAAQLAGLNVLRLLNEPTAAAIAYG 193 Query: 181 LDKAEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQRLIDY 240 LD + +G AVYDLGGGTFD+SI+++ FEVL+T GD+ LGG+DFD RL + Sbjct: 194 LDNSSEG--VFAVYDLGGGTFDISILKLT----RGVFEVLATGGDSALGGDDFDHRLFCW 247 Query: 241 IIEEFKKINGLDLSKDAIALQRIKASAERAKIELSSSQQTEINEPYIAMANGAPVHLNLK 300 I E + LS + L +KA K LS+ + +I+ + +G VHL+ Sbjct: 248 ISHEAQL---QPLSDEDTRLLMVKA--REVKELLSTKDEVQIDA---VLRSGEEVHLS-- 297 Query: 301 ITRAKLESLVEELIAKTIEPCRTAIKDAGVKVSDIDDVILVGGMTRMPKVIEKVKEFFGK 360 IT A+ + + L+AKT+ P R A++DAG+ V D+D V+LVGG TRMP + V EFF Sbjct: 298 ITAAQFNEITQHLVAKTMAPVRKALRDAGLTVEDVDGVVLVGGATRMPHIRRAVGEFFKT 357 Query: 361 DPRRDVNPDEAVAVGAAIQGSVLSGDRK---DVLLLDVTPLSLGIETMGGVMTKMIKKNT 417 +P +++PD+ VA+GAA+Q ++L+G+R D LLLDV PLSLGIETMGG+ K+I +N+ Sbjct: 358 NPLSNIDPDKVVALGAAVQANLLAGNRAPGDDWLLLDVIPLSLGIETMGGLAEKVIPRNS 417 Query: 418 TIPTKFSQVFSTADDNQPAVTIKVYQGEREMAAGNKALGEFNLEGIPPAPRGTPQIEVTF 477 TIP +Q F+T D Q A+ I V QGERE+ A ++L F L GIPP G +I VT+ Sbjct: 418 TIPCARAQEFTTFKDGQTAMAIHVVQGERELVADCRSLARFELRGIPPMAAGAARIRVTY 477 Query: 478 DIDANGILHVGAKDKATGKENKITIKANSGLNEEEIEKMVRDAEANAEEDKRLKELAESR 537 +DA+G+L V A++ +G E IT+K + GL ++EI +M++++ ++A+ED + + L E + Sbjct: 478 QVDADGLLAVSAREMTSGVEASITVKPSYGLGDDEIARMLQESFSSADEDMQRRALREEQ 537 Query: 538 NQGDALVHSTRKALGEYGDKLEAGEKDVIEAAIKNLEEALKGDDKAAIDAKTEALSTAAQ 597 + + +V +TR AL L E+ IEA ++ + A +G D AI A +AL+ + Sbjct: 538 VEAERIVLATRSALQADAALLSEAERQAIEAQVEAVSAAAQGTDHLAIKAAVDALAHGTE 597 Query: 598 KLGEKMYADQQAQAAAAG 615 + + D+ AA AG Sbjct: 598 EFAARRM-DRSVHAALAG 614 >gi|300312141|ref|YP_003776233.1| molecular chaperone, Hsp70 family [Herbaspirillum seropedicae SmR1] Length = 642 Score = 431 bits (1107), Expect = e-121, Method: Compositional matrix adjust. Identities = 251/620 (40%), Positives = 385/620 (62%), Gaps = 43/620 (6%) Query: 5 IGIDLGTTNSCVAVMEGNQPKVIENSEGARTTPSIIAYQEDGEI--------LVGAPAKR 56 +GIDLGTTNS VA ++ Q +++ + G PS++ Y + +VG AK Sbjct: 22 VGIDLGTTNSLVATVQSGQARILPDEAGQALLPSVVRYLPAHSLQPADRPPAVVGHAAKA 81 Query: 57 QAVTNPKNTIYAAKRLIGRKFTEKEVQKDINLMPYQIV---AADNGDAWIGVRDKKLAPP 113 +P+NT+ + KRL+GR + + MPY + D G++ P Sbjct: 82 AQSDDPRNTLSSIKRLMGRDRADISAGNSAD-MPYAFIDKPGLVQIDTAAGIK----TPI 136 Query: 114 QISAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDAQRQATKDAGRIAGLDVKRIINEPT 173 +SA++LR +++ AE LG E++ AVITVPAYF+DAQRQATKDA R+AGL+V R++NEPT Sbjct: 137 DVSADILRVLRQRAEASLGGELSGAVITVPAYFDDAQRQATKDAARLAGLNVLRLLNEPT 196 Query: 174 AAALAFGLDKAEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDF 233 AAA+A+GLD+A +G A+YDLGGGTFD+SI++++ FEVL+TNGD LGG+DF Sbjct: 197 AAAIAYGLDQAAEG--VFAIYDLGGGTFDISILKLS----RGVFEVLATNGDGALGGDDF 250 Query: 234 DQRLIDYIIEEFKKINGLDLSKDAIALQRIKASAERAKIELS--SSQQTEINEPYIAMAN 291 D+ + D+I+++ + ++D +R+ +A +AK LS +S E+N +++ Sbjct: 251 DRCIHDWIVDQSPLASAELTTQDT---RRLLTAARQAKETLSRETSATIELN-----LSS 302 Query: 292 GAPVHLNLKITRAKLESLVEELIAKTIEPCRTAIKDAGVKVSDIDDVILVGGMTRMPKVI 351 G V L L TR S+ + L+A+T+ P R A++DAG+ VS+ID V+LVGG TRMP+V Sbjct: 303 GQAVQLAL--TREVFASISQGLLARTLLPTRKALRDAGLGVSEIDGVVLVGGSTRMPQVR 360 Query: 352 EKVKEFFGKDPRRDVNPDEAVAVGAAIQGSVLSGDRK--DVLLLDVTPLSLGIETMGGVM 409 V +FFG+ P D++PD+ VA+GAA+Q +VL+G+R+ D LLLDV PLSLG+ETMGG++ Sbjct: 361 AAVADFFGQPPLTDLDPDQVVALGAAMQANVLAGNRQQSDWLLLDVCPLSLGLETMGGLV 420 Query: 410 TKMIKKNTTIPTKFSQVFSTADDNQPAVTIKVYQGEREMAAGNKALGEFNLEGIPPAPRG 469 K+I +N+TIP +Q F+T D Q A+++ V QGERE+ A ++L L GIPP G Sbjct: 421 EKIIPRNSTIPVARAQEFTTFKDGQTAMSVHVLQGERELVADCRSLARLELRGIPPMVAG 480 Query: 470 TPQIEVTFDIDANGILHVGAKDKATGKENKITIKANSGLNEEEIEKMVRDAEANAEEDKR 529 +I VTF IDA+G+L V A++++TG E +T+K + GL++ I +M+ +A +A +D + Sbjct: 481 AARIRVTFQIDADGLLSVSAREQSTGVEAAVTVKPSYGLDDANIARMLSEAVGHATDDMK 540 Query: 530 LKELAESRNQGDALVHSTRKALGEYGDK-LEAGEKDVIEAAIKNLEEALKG------DDK 582 ++ L E++ + +T AL G L A E+ I + L E ++ D+ Sbjct: 541 MRMLREAQVNARQVADATDAALKRDGHSLLSATEQAHIRQHLYALTELIETAREANDDNI 600 Query: 583 AAIDAKTEALSTAAQKLGEK 602 AA+ +AL+ A Q+ + Sbjct: 601 AALGRACDALNQATQEFAAR 620 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.131 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,825,645 Number of extensions: 77345 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 215 Number of HSP's successfully gapped: 3 Length of query: 646 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 555 Effective length of database: 1,188,985 Effective search space: 659886675 Effective search space used: 659886675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 134 (56.2 bits)