BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0556.p (181 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309929|ref|YP_003774021.1| lipoprotein B precursor transme... 325 2e-90 >gi|300309929|ref|YP_003774021.1| lipoprotein B precursor transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 181 Score = 325 bits (832), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 156/181 (86%), Positives = 168/181 (92%) Query: 1 MRAQLQIKRFSQWLLLVAAAVVLTACGFHLRGAVDLPFKTIYLGFGPNSPVGVELKRNLE 60 M AQ+QIKRF QWLL+VAAAV+L+ACGFHLRGA DLPFKTIYLGF PNSPVGVELKRN++ Sbjct: 1 MHAQVQIKRFPQWLLMVAAAVLLSACGFHLRGAADLPFKTIYLGFPPNSPVGVELKRNIQ 60 Query: 61 ASGAKVLRDPKEAEVNLKVLADTRDRQVLTLNTDGRVREYALFQRFTFSVTDAKGVIVIP 120 ASGA V+ D KEAE NLKVLAD+RDRQVLTLNT+GRVREYALFQ F+FSVTDAKG I+IP Sbjct: 61 ASGATVVDDAKEAEANLKVLADSRDRQVLTLNTNGRVREYALFQNFSFSVTDAKGAIIIP 120 Query: 121 PTAITLRRVITYDENQELAKQAEEVLLYRDMQSDLVQQILRRLSASKNAIHNEKAAADSD 180 PTAITLRRVITYDENQELAKQAEE LLYR+MQSDLVQQILRRLSASK AI NEKAAADSD Sbjct: 121 PTAITLRRVITYDENQELAKQAEETLLYRNMQSDLVQQILRRLSASKTAIKNEKAAADSD 180 Query: 181 S 181 S Sbjct: 181 S 181 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 463,321 Number of extensions: 16813 Number of successful extensions: 71 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 71 Number of HSP's successfully gapped: 1 Length of query: 181 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 102 Effective length of database: 1,245,805 Effective search space: 127072110 Effective search space used: 127072110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)