BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0535.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 739 0.0 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 80 3e-16 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 74 2e-14 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 71 1e-13 gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 66 5e-12 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 739 bits (1907), Expect = 0.0, Method: Compositional matrix adjust. Identities = 357/386 (92%), Positives = 375/386 (97%) Query: 1 MRALLYHSAHDVRVESVPDPIIQEADDIILKVSATAICGSDLHLYRGKIPATQAGDILGH 60 MRALLYHSAHDVRVE+VPDPIIQEADD+ILKV+ATAICGSDLHLYRGKIPAT+AGDILGH Sbjct: 1 MRALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRGKIPATKAGDILGH 60 Query: 61 EFMGVVVEAGPAVKRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGAIINGKGI 120 EFMGVVVEAGPA+KRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGA++N KGI Sbjct: 61 EFMGVVVEAGPAIKRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGALMNAKGI 120 Query: 121 RSGAAMFGYSHLYGGMPGGQAQYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK 180 RSGAAMFGYSHLYGGMPGGQA+YVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK Sbjct: 121 RSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK 180 Query: 181 NAGVGEGSSVAIYGAGPVGQMAAACARMLGAEKIFMVDHHDYRLRFAVEQYDVLPVNFDQ 240 NAGV EGSSVAIYGAGPVGQMAAA ARMLGAE+IFMVDHH YRLRFA EQYDV+PVNFDQ Sbjct: 181 NAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVIPVNFDQ 240 Query: 241 VDPAEYIIANTAGRGVDAVIDAVGFEAKGSALETAMTNLKLEGSSGQALRHCIASVRRGG 300 VDPAEYIIANTAGRGVD VIDAVGFEAKGS LETAMT LKLEGSSGQALRHCIASVRRGG Sbjct: 241 VDPAEYIIANTAGRGVDGVIDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASVRRGG 300 Query: 301 TLSVPGVYAGFIHGFLFGDIFEKGLTVKTGQTHVQHYMPELLASIEDGWLRPEAIITHRL 360 +SVPGVYAGFIHGFLFGDIFEKG+TVKTGQTHVQHYMPELLASIE+G LRP+AIITHRL Sbjct: 301 VISVPGVYAGFIHGFLFGDIFEKGVTVKTGQTHVQHYMPELLASIEEGRLRPDAIITHRL 360 Query: 361 PLEEAARGYRIFNEKQEECRKVVLTA 386 PLE+AARGY+IFN+K E+CRKVVLTA Sbjct: 361 PLEDAARGYQIFNDKLEDCRKVVLTA 386 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 79.7 bits (195), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 107/388 (27%), Positives = 154/388 (39%), Gaps = 50/388 (12%) Query: 1 MRALLYHSAHDVRVESVPDPI-IQEAD-------DIILKVSATAICGSDLHLYRGKIPAT 52 ++A + D +V P+ I+E D ++++++ A +C SDL + G P Sbjct: 3 IQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP-R 61 Query: 53 QAGDILGHEFMGVVVEAGPAVKRLKKGDRVVVPFVIACGQCFFCDQHLYAACE---TTNP 109 LGHE VVE G V L+ GD VV+ FV +CG C C + A CE TN Sbjct: 62 PVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATN- 120 Query: 110 DRGAIINGKGIRSGAAMFGYSHLYGGMPGGQAQYVRVP----KADVGPIRIDSALADEQV 165 G +++G+ + HL A R +AD+ P+ + Sbjct: 121 TVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPV--------QAA 172 Query: 166 LFLSDILPTGYQAVKNAGVGEGSSVAIYGAGPVGQMAAACARMLGAEKIFMVDHHDYRLR 225 LF +L AV A V G++ A+ G G VG A A GA ++ VD HD +L Sbjct: 173 LFGCAVLTGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLA 232 Query: 226 FAVEQYDVLPVNFDQVDPAEYIIANTAGRGVDAVIDAVGFEAKGSALETAMTNLKLEGSS 285 A VN D E + A T G GVD FE GS Sbjct: 233 LARSLGATATVNARDPDAVEKVRALTRG-GVD-----YAFEMAGSV-------------- 272 Query: 286 GQALRHCIASVRRGGTLSVPGVYAGFIHGFLFG-DIFEKGLTVK---TGQTHVQHYMPEL 341 QA+ RRGG G+ A H L + + TVK G +P Sbjct: 273 -QAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQVSLVAEERTVKGSYIGSCVPVRDIPRY 331 Query: 342 LASIEDGWLRPEAIITHRLPLEEAARGY 369 + + G L + ++ RL L + RG+ Sbjct: 332 IGLFQAGKLPVDRLMGERLALADINRGF 359 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 98/372 (26%), Positives = 156/372 (41%), Gaps = 43/372 (11%) Query: 24 EADDIILKVSATAICGSDLHLYRGKIPATQAGDILGHEFMGVVVEAGPAVKRLKKGDRVV 83 +A ++++++ AT IC +D + G P ILGHE GVVV+ GP VK LKK D V+ Sbjct: 26 KAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVKSLKKDDHVI 85 Query: 84 VPFVIACGQCFFCDQHLYAACETTNPDRGAII----NGKGIRSGAAMFGYSHLYGGMPGG 139 + C +C FC C+ +G + + G +F Y Sbjct: 86 PLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPDATSRFSLDGKPLFHYMGT-----ST 140 Query: 140 QAQYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK-NAGVGEGSSVAIYGAGPV 198 + Y+ VP+ + IR D+ ++V ++ + TG AV A V G++V ++G G + Sbjct: 141 FSNYIVVPEIALAKIRSDAPF--DKVCYIGCGVTTGVGAVLFTAKVEAGANVVVFGLGGI 198 Query: 199 GQMAAACARMLGAEKIFMVDHHDYRLRFAVEQYDVLPVNFDQVDPAEYIIANTAGRGVDA 258 G A+M+GA KI VD + R + + F D +I VDA Sbjct: 199 GLNVIQAAKMVGANKIIGVDLNPAR--------EAMARKFGMTD---FINPKDVPNVVDA 247 Query: 259 VIDAV------GFEAKGSALETAMTNLKLEGSSGQALRHCIASV-RRGGTLSVPGVYAGF 311 +I FE G+ + GQ++ +A+ + T V Sbjct: 248 IIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPFQLVTGRV 307 Query: 312 IHGFLFGDIFEKGLTVKTGQTHVQHYMPELLASIEDGWLRPEAIITHRLPLEEAARGYRI 371 G FG G+T V P+++ +G L + +ITH+LPLE G+ + Sbjct: 308 WKGSAFGG--------ARGRTDV----PKIVDWYMEGKLNIDDLITHKLPLERINEGFDL 355 Query: 372 FNEKQEECRKVV 383 + E R VV Sbjct: 356 M-KSGESIRSVV 366 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 71.2 bits (173), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 85/321 (26%), Positives = 128/321 (39%), Gaps = 64/321 (19%) Query: 2 RALLYHSAHDVRVESVPDPIIQEADDIILKVSATAICGSDLHLY--------RGKIPATQ 53 + L+ H+ HD+R++++ +Q + +KV A ICGSDLH Y R K P Sbjct: 3 QGLVIHAPHDLRIQALDTGALQP-QQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPM-- 59 Query: 54 AGDILGHEFMGVVVEAGPAVKRLKKGDRVVVPFVIACGQCFFC---------DQHLYAAC 104 +LGHE G + E G AV + G R+ + CG C +C D Y + Sbjct: 60 ---VLGHEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSA 116 Query: 105 ETTNPDRGAIINGKGIRSGAAMFGYSHLYGGMPGGQAQYVRVPKADVGPIRIDSALADEQ 164 T +GA I A H+ G ++A+A+ Sbjct: 117 MRTPHVQGAFRQEIIIERSQAHVVADHVSDG---------------------EAAMAEP- 154 Query: 165 VLFLSDILPTGYQAVKNAGVGEGSSVAIYGAGPVGQMAAACARMLGAEKIFMVDHHDYRL 224 L AV+ AG G V + G GP+G + AR GA ++ + D L Sbjct: 155 -------LSVALHAVRRAGPLVGKRVLVTGCGPIGALVVVAARRAGALEVVVTDVASMPL 207 Query: 225 RFAVEQYDVLPVNFDQVDPAEYIIANTAGRGVDAVIDAVGFEAKGSALETAMTNLKLEGS 284 + A++ +N + A + A A +G V+ FEA G+ E A+ LE Sbjct: 208 QAALQVGADSAINMAETPAA--LKAYAADKGTFDVL----FEASGN--ERALVG-ALEAL 258 Query: 285 SGQALRHCIASVRRGGTLSVP 305 QA+ I V GG + P Sbjct: 259 RPQAI---IVQVGLGGDIQFP 276 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 66.2 bits (160), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 70/267 (26%), Positives = 113/267 (42%), Gaps = 27/267 (10%) Query: 1 MRALLYHSAHDVRVESVPDPIIQEADDIILKVSATAICGSDLHLY-RGKIPA--TQAGDI 57 M+AL+ + ++++ + P A D+ +++ ICGSDLH Y G I +A + Sbjct: 1 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 Query: 58 LGHEFMGVVVEAGPAVKRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGAIING 117 LGHE G V+E G AV LK GDRV C + ++ RG Sbjct: 61 LGHEASGTVIEVGSAVSHLKVGDRV-------------CMEPGIPRLDSPATLRGMYNLD 107 Query: 118 KGIRSGAAMFGYSHLYGGMPGGQAQYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQ 177 +R A + L G + A R+P + + A+ ++ + L G Q Sbjct: 108 PAVRFWATPPIHGCLTGSVVHPAAFTYRLPD--------NVSFAEGAIV---EPLSIGLQ 156 Query: 178 AVKNAGVGEGSSVAIYGAGPVGQMAAACARMLGAEKIFMVDHHDYRLRFAVEQYDVLPVN 237 A A + G + + GAG +G M A A GA ++ + D +L + V+ V+ Sbjct: 157 AATKARMKPGDTAVVIGAGTIGAMTALAALAGGAARVILADVVAEKLAHFADNPAVITVD 216 Query: 238 FDQVDPAEYIIANTAGRGVDAVIDAVG 264 + + + T G G D V +A G Sbjct: 217 VTRETLTDVVRQATDGWGADVVFEASG 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,304,609 Number of extensions: 59117 Number of successful extensions: 210 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 204 Number of HSP's successfully gapped: 7 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)