BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0528.p (830 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 1208 0.0 gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 525 e-150 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 333 5e-92 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 281 2e-76 gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 105 1e-23 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 1208 bits (3126), Expect = 0.0, Method: Compositional matrix adjust. Identities = 617/852 (72%), Positives = 685/852 (80%), Gaps = 40/852 (4%) Query: 2 SKLSPSAPPMRPFAGPGSAVALPAAFLALDAQWRLLYLDEVAQVLLQA-SHAAAGQSVWQ 60 + LSP P P P AF ALD QWR ++LD AQ LL G+ +WQ Sbjct: 3 TALSPDRLPALP----------PEAFFALDPQWRFVFLDSPAQALLNPHGQDLLGRPIWQ 52 Query: 61 ACPALAGSALEESMRATLADGQSRQCRYLPPG-QP-YAYEVICYASTQGLSVSLRA---- 114 ACP L GS LEE +RA AD +SR + P QP AY V CYA GLSV LRA Sbjct: 53 ACPELLGSPLEECLRAVQADQRSRDAVLIHPHHQPGPAYAVSCYAGAHGLSVKLRAIAAQ 112 Query: 115 ------------------EVAGLTRASEQRRRFYETFLSTTPDLAYAWDLQHRFTYANRV 156 E+A LT SE+RRRFYETF +TTPDLAYAWDL+HRFTYANRV Sbjct: 113 PAAEHGLQQTRLRHEAVAELARLTDESERRRRFYETFFATTPDLAYAWDLEHRFTYANRV 172 Query: 157 LLQMWGKTWDEAIGKTCLELGYEPWHAEMHSREIEQVRATKRPVRGDVPFNGTFGRRIYD 216 LLQMWGKTWDEAIGKTCLELGYEPWHAEMHSREIEQVRATKRPVRGDVPFNGTFGRRIYD Sbjct: 173 LLQMWGKTWDEAIGKTCLELGYEPWHAEMHSREIEQVRATKRPVRGDVPFNGTFGRRIYD 232 Query: 217 YIIFPVLGPDGEVEAVAGTTRDVTERKNSEARQHALFRLAEAVRAAESLEAIATAAAHVI 276 YIIFPVLGPDGEVEAVAGTTRDVT+RK+SEARQHAL LA+A+RA +S + IA+AAAH+I Sbjct: 233 YIIFPVLGPDGEVEAVAGTTRDVTDRKHSEARQHALLHLADAIRATDSPQDIASAAAHII 292 Query: 277 GEALTARWVSYKTLGPQQQVEQLGQWSFHGSLDAVAAYTPLDLHDQRRLAQAVRSGSVVL 336 GEALTARWVS+ TLGPQ+Q + LGQW FH DA L H +R L + + G +L Sbjct: 293 GEALTARWVSFHTLGPQRQCQDLGQWRFHRDDDAERG---LQAHARRPLQRVLEQGHPML 349 Query: 337 VGDVASDARTAALRQQHLALGVASFMDVPLIDEGELVGIVHVSSNVPRTWTDNELAIVRE 396 V DV+SDART AL + ALGV SFMDVP++ + + V +V VSS+ PR WT+NELAIVRE Sbjct: 350 VADVSSDARTVALADEQRALGVVSFMDVPVLAQDQPVALVQVSSDAPRVWTENELAIVRE 409 Query: 397 VSQQTRAAFERIRNQQALAASEARLRQINESLEAEVSARTRELMATEEALRHSQKIEAIG 456 VS++ AAFERIRNQ+ALA SEARLR INESLEAEVSARTRELMATEEALRHSQKIEAIG Sbjct: 410 VSERACAAFERIRNQRALAESEARLRAINESLEAEVSARTRELMATEEALRHSQKIEAIG 469 Query: 457 QLTGGLAHDFNNLLGGISGSLDLMRVRLVQGRAEELLRYIDLAARSTQRAAALTQRLLAF 516 QLTGGLAHDFNNLLGGISGSLDLMRVRLVQGR EELLRY++LAARSTQRAA+LTQRLLAF Sbjct: 470 QLTGGLAHDFNNLLGGISGSLDLMRVRLVQGRGEELLRYVELAARSTQRAASLTQRLLAF 529 Query: 517 ARRQTLAPRPTNVNTLIGGMEELIRRTIGPAITLEVISAVGLWPTFVDGPQLESALLNLC 576 ARRQTLAP+PT VN LI GMEELIRRTIGPAI LEV+ A GLWPTFVDGPQLESALLNLC Sbjct: 530 ARRQTLAPKPTRVNALISGMEELIRRTIGPAIVLEVVGAGGLWPTFVDGPQLESALLNLC 589 Query: 577 INARDAMPDGGRLTIETTNKWLDDKWAQERDLPVGQYVCIAVTDNGTGMTPEVMARAFDP 636 INARDAMP GGRLTIET NKWLDD+WA ERDLP GQYVCI+VTD+G GM P+V+ARAFDP Sbjct: 590 INARDAMPGGGRLTIETANKWLDDRWAHERDLPPGQYVCISVTDSGVGMPPDVLARAFDP 649 Query: 637 FFTTKPIGEGTGLGLSMVYGFARQSGGQVRLYSELGQGSTVSLYLPRHQHAAQAEECEPA 696 FFTTKPIG GTGLGLSMVYGFARQSGGQVRLYSE+GQG+TVS+YLPRHQH ++ A Sbjct: 650 FFTTKPIGAGTGLGLSMVYGFARQSGGQVRLYSEVGQGTTVSIYLPRHQHDGDEQDEADA 709 Query: 697 HLPSAA--GRGEVVLVVDDEASIRLLLVEVLSEAGYTTLQAEDGPAALRILQSAAQIDLL 754 +AA G GEVVLVVDDEASIR+L+ E+LS+AGY LQAEDGP ALRILQS +IDLL Sbjct: 710 LDETAAGDGNGEVVLVVDDEASIRMLVAEILSDAGYDVLQAEDGPGALRILQSKQRIDLL 769 Query: 755 ITDVGLPGGLNGRQVADAARTARPGLQVLFITGYAENAALGNGHLQPGMQVLTKPFKMDT 814 ITDVGLPGGLNGRQVADA R ARP L VLFITGYAENAA NGHL+ GMQ++TKPFKM+ Sbjct: 770 ITDVGLPGGLNGRQVADAGRAARPDLHVLFITGYAENAAFDNGHLERGMQLMTKPFKMEA 829 Query: 815 LSQRVQAILDSR 826 L++RVQ+IL+SR Sbjct: 830 LARRVQSILESR 841 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 525 bits (1353), Expect = e-150, Method: Compositional matrix adjust. Identities = 262/430 (60%), Positives = 336/430 (78%), Gaps = 9/430 (2%) Query: 397 VSQQTRAAFERIRNQQALAASEARLRQINESLEAEVSARTRELMATEEALRHSQKIEAIG 456 + + R A E R++ ALAA LR +NE+LE V RT L+ EEALR +QK+EA+G Sbjct: 257 IDIEGRRAVEAERDR-ALAA----LRTLNETLEQRVEERTAALLNAEEALRQAQKMEAVG 311 Query: 457 QLTGGLAHDFNNLLGGISGSLDLMRVRLVQGRAEELLRYIDLAARSTQRAAALTQRLLAF 516 QLTGGLAHDFNN+L GISGSL+LM+VRL QGR ++ R+++ A + +RAAALTQRLLAF Sbjct: 312 QLTGGLAHDFNNILAGISGSLELMKVRLAQGRIGDIERHLNGAQSAVKRAAALTQRLLAF 371 Query: 517 ARRQTLAPRPTNVNTLIGGMEELIRRTIGPAITLEVISAVGLWPTFVDGPQLESALLNLC 576 +RRQTL P+P ++N ++ GM ELI R++GPAI +E I+A GLW TFVD Q+E+ALLNLC Sbjct: 372 SRRQTLDPKPADLNRIVAGMHELIGRSVGPAIAVETIAAGGLWHTFVDIGQMENALLNLC 431 Query: 577 INARDAMPDGGRLTIETTNKWLDDKWAQERDLPVGQYVCIAVTDNGTGMTPEVMARAFDP 636 INARDAMP GGRLTIET N+WLDD A +R +P GQYV ++V+D G GM+PEV+ARAFDP Sbjct: 432 INARDAMPHGGRLTIETANRWLDDIGALQRGVPPGQYVSLSVSDTGAGMSPEVVARAFDP 491 Query: 637 FFTTKPIGEGTGLGLSMVYGFARQSGGQVRLYSELGQGSTVSLYLPRHQHAAQAEEC--- 693 FFTTKP G+GTGLGLSMVYGFA QSGG VR+YSELGQG+ V +YLPRH ++ Sbjct: 492 FFTTKPTGQGTGLGLSMVYGFAGQSGGTVRIYSELGQGAMVCIYLPRHVGEGGVDDLALP 551 Query: 694 EPAHLPSAAGRGEVVLVVDDEASIRLLLVEVLSEAGYTTLQAEDGPAALRILQSAAQIDL 753 E +H+P+A G + +LVVDDE +R++ VEVL + GY+ L+AEDGP+ALR++++ +IDL Sbjct: 552 EESHVPAAPG-SKTILVVDDEPLVRMVTVEVLRDLGYSVLEAEDGPSALRVVEACQEIDL 610 Query: 754 LITDVGLPGGLNGRQVADAARTARPGLQVLFITGYAENAALGNGHLQPGMQVLTKPFKMD 813 L+TDVGLP G+NGRQ+ADA R RP L VLF+TGYAENA L +GHL+ GMQVLTKPF D Sbjct: 611 LLTDVGLPNGMNGRQLADAIRAPRPDLPVLFVTGYAENAVLNHGHLERGMQVLTKPFAAD 670 Query: 814 TLSQRVQAIL 823 L++RV+ ++ Sbjct: 671 VLARRVKELV 680 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 333 bits (853), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 177/416 (42%), Positives = 265/416 (63%), Gaps = 9/416 (2%) Query: 417 SEARLRQINESLEAEVSARTRELMATEEALRHSQKIEAIGQLTGGLAHDFNNLLGGISGS 476 +E LR++N++LE V E E ALR QK+EA+GQLTGG+AHDFNN+L I G+ Sbjct: 688 AEEALRELNDTLEQRVVEEILERSRAEAALRQMQKMEAVGQLTGGVAHDFNNVLQIIGGN 747 Query: 477 LDLMRVRLVQGRAEELLRY-IDLAARSTQRAAALTQRLLAFARRQTLAPRPTNVNTLIGG 535 L L++ R +E R + +A + R A L+ +LLAFARRQ L PR N+ ++ Sbjct: 748 LQLLQS---DPRLDERSRQRVRIAIGAVDRGAKLSSQLLAFARRQPLQPRSINLERVVQN 804 Query: 536 MEELIRRTIGPAITLEVISAVGLWPTFVDGPQLESALLNLCINARDAMPDGGRLTIETTN 595 M++L+RR +G I + A LW T +D Q+E+ ++N INARDAMP GGRLT++ N Sbjct: 805 MDDLLRRAVGENIDISTRVAHDLWNTMLDPNQVENLIINTAINARDAMPSGGRLTVQIDN 864 Query: 596 KWLDDK-WAQERDLPVGQYVCIAVTDNGTGMTPEVMARAFDPFFTTKPIGEGTGLGLSMV 654 L+D + + DL G+YV + ++DNG GM+PEV RAF+PFFTTK GEGTGLGLSM Sbjct: 865 VRLEDPVFLSQADLAPGEYVELQISDNGAGMSPEVRDRAFEPFFTTKLEGEGTGLGLSMA 924 Query: 655 YGFARQSGGQVRLYSELGQGSTVSLYLPRH-QHAAQAEECEPAHLPSAAGRGEVVLVVDD 713 YGF +QS G + ++SE G+G+T+ +Y PR + +E + G E +LVV+D Sbjct: 925 YGFVKQSKGHISIHSEQGKGTTIRIYFPRCLDEEVRIDEVRDL---TVHGGDETILVVED 981 Query: 714 EASIRLLLVEVLSEAGYTTLQAEDGPAALRILQSAAQIDLLITDVGLPGGLNGRQVADAA 773 + +++L +++ LS GY L+A++G AL ILQS IDL+ TDV +PG + ++A A Sbjct: 982 DPNLQLTVIDTLSSLGYRVLKADNGERALEILQSGVAIDLVFTDVVMPGSVPVTELAQQA 1041 Query: 774 RTARPGLQVLFITGYAENAALGNGHLQPGMQVLTKPFKMDTLSQRVQAILDSRGKR 829 R P +++LF +GY + + G L G+++L+KP++++ L+QR++ +L R R Sbjct: 1042 RLLVPDIEILFTSGYPRDTIVHEGRLDAGVELLSKPYRINQLAQRIRQMLSQRAHR 1097 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 281 bits (718), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 165/385 (42%), Positives = 235/385 (61%), Gaps = 5/385 (1%) Query: 443 EEALRHSQKIEAIGQLTGGLAHDFNNLLGGISGSLDLMRVRLVQGRAE--ELLRYIDLAA 500 E+AL ++K+EA+GQLTGG+AHDFNNLL I+G LD++ + L + + + R D Sbjct: 164 EQALGQARKMEALGQLTGGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADSIR 223 Query: 501 RSTQRAAALTQRLLAFARRQTLAPRPTNVNTLIGGMEELIRRTIGPAITLEVISAVGLWP 560 +++ +AA LTQ+LLAFAR+Q L R N+N+L G +L+ RT+G IT++ A GLW Sbjct: 224 KASGKAAMLTQQLLAFARKQRLEGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGLWN 283 Query: 561 TFVDGPQLESALLNLCINARDAMPDGGRLTIETTNKWL-DDKWAQERDLPVGQYVCIAVT 619 +D Q+E ALLN+ INARDAM GR+ I T N+ L D A + L G YV IAVT Sbjct: 284 CRLDPTQMEVALLNVLINARDAMNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIAVT 343 Query: 620 DNGTGMTPEVMARAFDPFFTTKPIGEGTGLGLSMVYGFARQSGGQVRLYSELGQGSTVSL 679 D+G G+ P+++ R DPFFTTK G+GTGLGLSMVYGF +QSGG VR+ S G+TV++ Sbjct: 344 DDGPGIAPDILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTI 403 Query: 680 YLPRHQHAAQAEECEPAHLPSAAGRGEVVLVVDDEASIRLLLVEVLSEAGYTTLQAEDGP 739 Y P Q E + + G E +LVVDD + L +L GY Sbjct: 404 YFPATQDQVGG-GFEYDNRSQSQGGQEHILVVDDRLEVAELAQTMLESLGYRVSMVNSPA 462 Query: 740 AALRILQSAAQIDLLITDVGLPGGLNGRQVADAARTARPGLQVLFITGYAENAALGNGHL 799 AL++++S +DLL TD+ +PG +NG +A A+ P ++VL TGYA + +G Sbjct: 463 EALQLIRSGRPVDLLFTDLIMPGSMNGVVLAREAKRMLPTIKVLLTTGYASESLERHGA- 521 Query: 800 QPGMQVLTKPFKMDTLSQRVQAILD 824 VL KP++ D L+++++ +LD Sbjct: 522 DGEFPVLDKPYRHDELARKIRIVLD 546 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 105 bits (262), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 81/281 (28%), Positives = 130/281 (46%), Gaps = 52/281 (18%) Query: 438 ELMATEEALRHSQKIEAIGQLTGGLAHDFNNLLGGISGSLD---------LMRVRLVQ-- 486 +L + L S+K+ +IGQL G+AH+ NN +G +S +L+ L ++L Q Sbjct: 170 QLKMAQAQLMQSEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQLHQCL 229 Query: 487 -------GRAEELLRYI----DLAARST-------------QRAAALTQRLLAFARRQTL 522 A+E LR DLA S QR + Q L F+R Sbjct: 230 QGGALPAAMAQEQLRSFEARADLAFLSEDVTELLAESNEGLQRVRNIVQSLKDFSRIGES 289 Query: 523 APRPTNVNTLIGGMEELIRRTIGPAITLEVISAVGLWPTF-VDGPQLESALLNLCINARD 581 + T+++ + + + ++ ++ G P +L +NL +NA Sbjct: 290 NWQMTDLHRGLDSTLNIANNELKYKVS--IVKQYGELPLVKCLASELNQVFMNLLVNAAQ 347 Query: 582 AMPDGGRLTIETTNKWLDDKWAQERDLPVGQYVCIAVTDNGTGMTPEVMARAFDPFFTTK 641 A+ + G +TI T G V ++++D G G+ PE + R F+PFFTTK Sbjct: 348 AISERGTVTICTAR--------------AGDKVSVSISDTGHGIAPENLNRIFEPFFTTK 393 Query: 642 PIGEGTGLGLSMVYGFARQSGGQVRLYSELGQGSTVSLYLP 682 P+G GTGLGLS+ YG ++ GG +R+ S G G+T ++ LP Sbjct: 394 PVGSGTGLGLSLSYGIVKKHGGDIRVSSAPGCGTTFTIELP 434 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,642,735 Number of extensions: 115501 Number of successful extensions: 631 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 622 Number of HSP's successfully gapped: 17 Length of query: 830 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 737 Effective length of database: 1,179,515 Effective search space: 869302555 Effective search space used: 869302555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)