BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0524.p (587 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 909 0.0 gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 594 e-171 gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 106 6e-24 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 909 bits (2350), Expect = 0.0, Method: Compositional matrix adjust. Identities = 457/560 (81%), Positives = 481/560 (85%), Gaps = 10/560 (1%) Query: 1 MKSIPAIDYQQQRFGYQPCAEQTRPDQAAVHPVVVIGAGPVGLVTAIDLARQGIAVVLVD 60 M PAIDYQQQRF + PCAEQ P A VHPVV+IGAGPVGL AIDLARQGIAVVLVD Sbjct: 1 MNQNPAIDYQQQRFAWTPCAEQQSPGHAPVHPVVIIGAGPVGLACAIDLARQGIAVVLVD 60 Query: 61 DDDTLSTGSRAICFAKRTLDIFDRIGCGQRMVDKGVGWSRGKVFLRQEAVYEFDLLPESG 120 DDDTLSTGSRAICFAKRTLDIFDRIGCGQRMVDKGVGW+RGKVFLR +AVYEFDLLPESG Sbjct: 61 DDDTLSTGSRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLRDQAVYEFDLLPESG 120 Query: 121 HARPAFINLQQYYVEGYLLEQARQFEHIELRWKSKVSAITHHADHEALTVDTPQGSYTLK 180 HARPAFINLQQYYVEGYLLEQA+Q+ HIE+RWK+KVSAI HH DHEALTVDTPQGSYTLK Sbjct: 121 HARPAFINLQQYYVEGYLLEQAQQWPHIEIRWKNKVSAIAHHEDHEALTVDTPQGSYTLK 180 Query: 181 AQYVVAADGSRSALRKMMGLESLGHTFRDRFLIADVKMAAPFPAERWFWFDPPFHPNQSV 240 A+YVVAADGSRS LRKMMGLES GHTFRDRFLIADVKM APFPAERWFWFDPPFHP QSV Sbjct: 181 ARYVVAADGSRSPLRKMMGLESQGHTFRDRFLIADVKMDAPFPAERWFWFDPPFHPQQSV 240 Query: 241 LLHRQPDDVWRIDFQLGWEADPVLEKSPERVIPRVQALLGKEVQFSLEWVSVYTFSCVRM 300 LLHRQPDDVWRIDFQLGW+ADPVLEK+PERVIPRV+ALLG+EV FSLEWVSVYTFSCVRM Sbjct: 241 LLHRQPDDVWRIDFQLGWDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRM 300 Query: 301 DRFRHGRVLFVGDAAHCVSPFGARGANSGVQDAENLAWKLAYVLQGKAPDSLLDSYASER 360 +RFRHGRVLFVGDAAHCVSPFGARGANSG+QDAENLAWKLA+VLQGKAPDSLLDSYASER Sbjct: 301 ERFRHGRVLFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASER 360 Query: 361 EYAADENLLNSTRSTDFITPKSPVSRRLRDAVLGLARDHAFARRLVNSGRLSLPATLHGS 420 EYAADENLLNSTRSTDFITPKS VSR RDAVLGLAR+HAFARRLVNSGRLSLPATL S Sbjct: 361 EYAADENLLNSTRSTDFITPKSAVSRAFRDAVLGLAREHAFARRLVNSGRLSLPATLDQS 420 Query: 421 VLNTEDSA------PFQGAMQAGAPCTDAPVVSDTGADWLLRQLGERFVAMIFAAPAALP 474 LNT D A F G M+ GAPC DAP++ WLL QLG+RFV MIFAAPA LP Sbjct: 421 PLNTPDQAAAPEAVAFTGPMRPGAPCCDAPLLQAGQPAWLLPQLGDRFVLMIFAAPAQLP 480 Query: 475 AQQVQALAQLTQGALPLQLLFVGQAAATPEDRHVLAHLGQAARYVQDIEALAAQRYDAAH 534 A QALA L Q L+LLF+ AAT ED VL L A VQDI+ LAAQRYDA Sbjct: 481 AATRQALAALAQPDA-LRLLFISAQAATQEDERVLQTLAGQAIQVQDIDTLAAQRYDAHP 539 Query: 535 ASSQTCYLIRPDQHVCARWR 554 S CYLIRPDQHV ARWR Sbjct: 540 GS---CYLIRPDQHVSARWR 556 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 594 bits (1532), Expect = e-171, Method: Compositional matrix adjust. Identities = 310/564 (54%), Positives = 379/564 (67%), Gaps = 28/564 (4%) Query: 9 YQQQRFGYQPCAEQTRPDQAAVHPVVVIGAGPVGLVTAIDLARQGIAVVLVDDDDTLSTG 68 Y RF Y+ EQ R + + HPVVVIGAGP+GL A++ A +G+ VV++DD+DT+S G Sbjct: 5 YTYPRFAYRQSDEQ-REARPSRHPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTVSIG 63 Query: 69 SRAICFAKRTLDIFDRIGCGQRMVDKGVGWSRGKVFLRQEAVYEFDLLPESGHARPAFIN 128 SRA+C+AKR L+I+DR+G RMV+KGV W GKVF + VY FDLLPE H PA IN Sbjct: 64 SRAVCYAKRPLEIWDRLGVAPRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPAMIN 123 Query: 129 LQQYYVEGYLLEQARQFEHIELRWKSKVSAITHHADHEALTVDTPQGSYTLKAQYVVAAD 188 LQQYY+E YL+E + I+LRWK K+ ++ H L V+TP G + ++A +V+A D Sbjct: 124 LQQYYLEQYLVEACQAQPLIDLRWKHKMISLHQDDTHATLDVETPDGIFRMEADWVIACD 183 Query: 189 GSRSALRKMMGLESLGHTFRDRFLIADVKMAAPFPAERWFWFDPPFHPNQSVLLHRQPDD 248 G+ S RKM+G E G F+DRFLIADV M A FPAERWFWFDPPFH QSVLLH+Q D+ Sbjct: 184 GANSDTRKMVGAEFSGQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQCDN 243 Query: 249 VWRIDFQLGWEADPVLEKSPERVIPRVQALLGKEVQFSLEWVSVYTFSCVRMDRFRHGRV 308 VWRIDFQLGWEADP EK PE+VIPR+QA+LGKEV+F LEWVSVY F+C R+D+ R+ RV Sbjct: 244 VWRIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRYQRV 303 Query: 309 LFVGDAAHCVSPFGARGANSGVQDAENLAWKLAYVLQGKAPDSLLDSYASEREYAADENL 368 LF GDAAH VSPFGARGAN+GVQD +NL WKL V+ GKAP S +DSY ER +AAD+NL Sbjct: 304 LFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPPSFIDSYHEERAWAADDNL 363 Query: 369 LNSTRSTDFITPKSPVSRRLRDAVLGLARDHAFARRLVNSGRLSLPATLHGSVLNTEDSA 428 STRSTDFITPKS S+ LRDAVLGLA FAR LVNSGRLS P + S LNT D+A Sbjct: 364 RQSTRSTDFITPKSRASKALRDAVLGLAETEEFARTLVNSGRLSTPTSYVHSPLNTADAA 423 Query: 429 PFQGAMQAGAPCTDAPVVSDTGADWLLRQLGERFVAMIFAAPAALPAQQVQALAQLTQGA 488 F G M G C DAPV+ + WLL LG FV + F A A P ++A Sbjct: 424 LFGGRMAPGTVCADAPVLREGQPGWLLESLGAGFVVLFFGASA--PQAVIEA------AG 475 Query: 489 LPLQLLFVGQAAATPEDRHVLAHLGQAARYVQDIEALAAQRYDAAHASSQTC-YLIRPDQ 547 + +++ VG + E + D E LAA RY A+S+ C YL+RPDQ Sbjct: 476 ISARVVTVGAPGSGAE--------------LIDREGLAALRY----AASEGCVYLVRPDQ 517 Query: 548 HVCARWRRVDAPAIGAALLRASGN 571 HV ARW R + I AAL R G Sbjct: 518 HVAARWNRFHSDDIAAALARCVGQ 541 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 106 bits (264), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 100/354 (28%), Positives = 157/354 (44%), Gaps = 22/354 (6%) Query: 15 GYQPCAEQTRPDQAAVHPVVVIGAGPVGLVTAIDLARQGIAVVLVDDDDTLSTGSRAICF 74 G QP P Q V+++GAGPVG+ L GI+ +L++ + + RA+ Sbjct: 5 GQQPAV----PAQVMQAQVLIVGAGPVGVTLGNLLGTYGISTLLIERNLDVLDYPRAVGI 60 Query: 75 AKRTLDIFDRIGCGQRMVDKGVGWSRGKVFLRQEAVYEFDLLP---ESGHARPAFINLQQ 131 L F G +V + +++ + ++ P E G R + Q Sbjct: 61 DDEALRTFQSAGMADAIVRDVIQNVPMRMYTADRRCFA-EIQPTTREFGWYRRNLFS--Q 117 Query: 132 YYVEGYLLEQARQFEHIELRWKSKV-SAITHHADHEALTVDTPQGSYTLKAQYVVAADGS 190 E L R+FEH+ LR ++ + A +A +D TL+AQYVV DG Sbjct: 118 PLGEATLRLGLRRFEHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGG 177 Query: 191 RSALR-KMMGLESLGHTFRDRFLIADVK---MAAPFPAERWFWFDPPFHPNQSVLLHRQP 246 RS +R K++ L G T ++++ + + AP+ A P + + P Sbjct: 178 RSTVREKILKLPYEGKTHPAKWVVIECDGDPLDAPYTALH-------CEPQRPYVCLALP 230 Query: 247 DDVWRIDFQLGWEADPVLEKSPERVIPRVQALLGKEVQFSLEWVSVYTFSCVRMDRFRHG 306 V R +F L D +PE+V ++ + ++ VYT + G Sbjct: 231 YGVRRWEFMLFPGEDGEQMLAPEKVRELLREHVRDVDALNIIRARVYTHNSRVAGSLVCG 290 Query: 307 RVLFVGDAAHCVSPFGARGANSGVQDAENLAWKLAYVLQGKAPDSLLDSYASER 360 R+ GDAAH P+ +G N+G++DA NLAWKLA+VLQG+ LL SY ER Sbjct: 291 RICLAGDAAHITPPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEER 344 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,888,488 Number of extensions: 80048 Number of successful extensions: 306 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 300 Number of HSP's successfully gapped: 3 Length of query: 587 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 497 Effective length of database: 1,193,720 Effective search space: 593278840 Effective search space used: 593278840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)