BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0522.p (455 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 749 0.0 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 365 e-102 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 349 3e-97 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 335 5e-93 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 298 6e-82 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 749 bits (1935), Expect = 0.0, Method: Compositional matrix adjust. Identities = 401/451 (88%), Positives = 417/451 (92%), Gaps = 2/451 (0%) Query: 2 MTPSPGATTLAAPPHLNLSVAAEDAIYRKVWLRIIPFLFLCYVVSFLDRINIGFAQLQMK 61 MTPS G TL+ P +L+ AEDAIYRKVWLRIIPFLF+CYVVSFLDRINIGFAQLQMK Sbjct: 1 MTPSSGGATLSTTP--SLASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMK 58 Query: 62 QDLGFSDAMYGLGAAVFYVGYVLCEVPSNMLLARFGARRTFSRIMVLWGLASVGMMAVTD 121 QDLGFSDAMYGLGAA+FYVGYVL EVPSNM LARFGARRTFSRIMVLWGLASVGMMAVTD Sbjct: 59 QDLGFSDAMYGLGAAIFYVGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTD 118 Query: 122 ATHFYVLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWI 181 A HFY LRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWI Sbjct: 119 AAHFYTLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWI 178 Query: 182 MRDMAGVMGMYGWQWMFAIEGAPAVVLGVLVIFLLADGPAQASWLSPQERSFLLARHTQE 241 MRDMAGVMG+YGWQWMFAIEGAPAVVLG+L IFLLADGPAQA WLSP+ERSFL ARH QE Sbjct: 179 MRDMAGVMGLYGWQWMFAIEGAPAVVLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQE 238 Query: 242 TQRSQHVKAHSLTALRHALSHRPTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVQV 301 R+ HV HSL ALR ALSHR TYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVV+V Sbjct: 239 ALRTHHVSGHSLAALRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEV 298 Query: 302 SLYTVIPNAIGAIGLILIARRSDRRNERHGHFLFCTTGGALALAALTLHPSSLAVSLGLL 361 SLY+VIPNAIGA+GLILIARRSDRRNERHGHF CTTGGALALAALTLHPSS AVSLGLL Sbjct: 299 SLYSVIPNAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLL 358 Query: 362 SLAAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLIKTRTGNID 421 S+AAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGL+KTRTGN+D Sbjct: 359 SVAAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGNMD 418 Query: 422 IALYILAGLLLLSGLTMWGLIPRKATQEAQS 452 IALYILAGLLLLSGL MW LIPRKA A + Sbjct: 419 IALYILAGLLLLSGLAMWSLIPRKAATAATA 449 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 365 bits (936), Expect = e-102, Method: Compositional matrix adjust. Identities = 189/413 (45%), Positives = 265/413 (64%), Gaps = 6/413 (1%) Query: 24 EDAIYRKVWLRIIPFLFLCYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAVFYVGYV 83 EDA Y KV R++P LFLCYV S+LDR+N+GFA+LQM QDL FSD +YGLGA +F++GY Sbjct: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 Query: 84 LCEVPSNMLLARFGARRTFSRIMVLWGLASVGMMAVTDATHFYVLRFLLGVFEAGFFPGI 143 + EVPSNM+L + GAR +RIM+ WG+ S M VT FY++RFLLGV EAGFFPG+ Sbjct: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 Query: 144 VLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGMYGWQWMFAIEGA 203 +LYL+YW+PA RR + A+F G+A++GV+GG +SGWIM M GV G GWQWMF +E Sbjct: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 Query: 204 PAVVLGVLVIFLLADGPAQASWLSPQERSFLLARHTQETQRSQHVKAHSLTALRHALSHR 263 P+++LGV+VI + D A WLS +E+ L A + E + +H+ SL A+ ++ Sbjct: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKEHL---SLGAM---FANG 250 Query: 264 PTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAIGLILIARRS 323 +L A IYF G ++FW+P +I+ G+ D + V L T IP A A ++LI R + Sbjct: 251 RVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSA 310 Query: 324 DRRNERHGHFLFCTTGGALALAALTLHPSSLAVSLGLLSLAAVLIFAALPIFWSLPPGLL 383 D R ER H G + L T + + +++ L+LA+V I LP+FWSLP L Sbjct: 311 DARRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFL 370 Query: 384 PAQTIAPGIAFISSVGITSGIVSPWLIGLIKTRTGNIDIALYILAGLLLLSGL 436 A GIA I+S+G +G VSP+L+G +K T + + +Y+LA L++ GL Sbjct: 371 SGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGL 423 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 349 bits (895), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 181/420 (43%), Positives = 263/420 (62%), Gaps = 5/420 (1%) Query: 17 LNLSVAAEDAIYRKVWLRIIPFLFLCYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAA 76 L+++ ++D +Y+KV+ RI+PFL LCYV+++LDR+N+GFA+LQM QDLGFS+ ++GLGA Sbjct: 11 LDVNDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAG 70 Query: 77 VFYVGYVLCEVPSNMLLARFGARRTFSRIMVLWGLASVGMMAVTDATHFYVLRFLLGVFE 136 +F++GY L EVPSN+L+ + GAR +RIM+ WG+ S M V +AT FY+LRFLLG+ E Sbjct: 71 LFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAE 130 Query: 137 AGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGMYGWQW 196 AGF+PGI+LYL+YWFP+ RRA V+A+F +G+ VAG+LG +SGWIM G+ GWQW Sbjct: 131 AGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQW 190 Query: 197 MFAIEGAPAVVLGVLVIFLLADGPAQASWLSPQERSFLLARHTQETQRSQHVKAHSLTAL 256 MF IE PAV++GV + L + A WL+ +E++ L A + + + H A+ Sbjct: 191 MFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKE--SKHGFGAI 248 Query: 257 RHALSHRPTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAIGL 316 + +L IYF+ G L WMP +++ G+K +++ L + IP I + Sbjct: 249 ---VKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAM 305 Query: 317 ILIARRSDRRNERHGHFLFCTTGGALALAALTLHPSSLAVSLGLLSLAAVLIFAALPIFW 376 LI R +DR ER H + G + L + AVS+ LSLAA + P+FW Sbjct: 306 NLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFW 365 Query: 377 SLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLIKTRTGNIDIALYILAGLLLLSGL 436 SLP L A GIA I+SVG +G VSP+LIG +K T N +Y+LA +L++ + Sbjct: 366 SLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSI 425 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 335 bits (859), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 174/410 (42%), Positives = 262/410 (63%), Gaps = 5/410 (1%) Query: 25 DAIYRKVWLRIIPFLFLCYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAVFYVGYVL 84 + YRK+ R+IPFL +V++++DR+N+GFA+LQM QDL FS+A+YGLGA +F++GY + Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 85 CEVPSNMLLARFGARRTFSRIMVLWGLASVGMMAVTDATHFYVLRFLLGVFEAGFFPGIV 144 EVPSN+LL + GAR+T +RI +LWGLAS+ M VT T FY+LRFLLG+FEAGFFPG+V Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 145 LYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGMYGWQWMFAIEGAP 204 LYL+YWFPA+RRA V +F A+AG +GG ++G IM M GV + WQW+F +EG P Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 205 AVVLGVLVIFLLADGPAQASWLSPQERSFLLARHTQETQRSQHVKAHSLTALRHALSHRP 264 +++ G V+ L + PA A WLS E++ + +++ + + A ++ Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKN-----AVTQVLAAENSAPGKHFSFKDACTNYR 256 Query: 265 TYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAIGLILIARRSD 324 ++ A +YF + G+ T+ FW P +I++ G+K +Q+ L + IP G + +I SD Sbjct: 257 VWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSD 316 Query: 325 RRNERHGHFLFCTTGGALALAALTLHPSSLAVSLGLLSLAAVLIFAALPIFWSLPPGLLP 384 + ER H T + L A + + ++L L+LAA+ I AA P+FWS+P L Sbjct: 317 KTGERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLA 376 Query: 385 AQTIAPGIAFISSVGITSGIVSPWLIGLIKTRTGNIDIALYILAGLLLLS 434 A GIA I+S+G +G V+P++IG +KT TG++ LY +A L L++ Sbjct: 377 GTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVA 426 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 298 bits (763), Expect = 6e-82, Method: Compositional matrix adjust. Identities = 165/422 (39%), Positives = 254/422 (60%), Gaps = 17/422 (4%) Query: 25 DAIYRKVWLRIIPFLFLCYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAVFYVGYVL 84 D +Y+KV +I+P+L L YV+++LDR+N+GFA+L M DLG S+A YGLGA +F++GY + Sbjct: 13 DGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFFLGYFI 72 Query: 85 CEVPSNMLLARFGARRTFSRIMVLWGLASVGM---------MAVTDATHFYVLRFLLGVF 135 EVPSN++L R GA+ +RIM+ WG+ S M + V++ T FY LRFLLG Sbjct: 73 FEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYTLRFLLGAS 132 Query: 136 EAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGMYGWQ 195 EAGFFPG++LY++YW+PA + VMA+ G + +LGG +SG +M+ M + GWQ Sbjct: 133 EAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGDSLAGWQ 192 Query: 196 WMFAIEGAPAVVLGVLVIFLLADGPAQASWLSPQERSFLLARHTQETQRSQHVKAHSLTA 255 WMF IE PA +LG++++F+L++ A A WL+P+E++ L R E + +V SL A Sbjct: 193 WMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEKVNVPLKSLYA 252 Query: 256 LRHALSHRPTYLF-AFIYFALTCGSLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAI 314 L P F A +Y + GS +NFW P +IRD G+K V+ + + + IP +GA Sbjct: 253 L-------PILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGAT 305 Query: 315 GLILIARRSDRRNERHGHFLFCTTGGALALAALTLHPSSLAVSLGLLSLAAVLIFAALPI 374 ++ I R +++ + + + + LAL TL P ++ ++L +++A ++ + Sbjct: 306 AMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIAL 365 Query: 375 FWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLIKTRTGNIDIALYILAGLLLLS 434 FW P T A GIA I+SVG G V P+L+G + T G+ L +L G L L+ Sbjct: 366 FWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGALFLA 425 Query: 435 GL 436 G+ Sbjct: 426 GV 427 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.141 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,171,888 Number of extensions: 42596 Number of successful extensions: 263 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 235 Number of HSP's successfully gapped: 21 Length of query: 455 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 367 Effective length of database: 1,203,190 Effective search space: 441570730 Effective search space used: 441570730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)