BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0517.p (484 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 729 0.0 gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 309 3e-85 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 131 1e-31 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 129 4e-31 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 127 2e-30 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 729 bits (1883), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/484 (85%), Positives = 443/484 (91%) Query: 1 MELFSDLPGDEGLPGQQRRRAMLAVMTATTMAVFDGTIINVALPQISLALHTSAAASVWV 60 MELFSD P DEGLPG+QRRRAMLAVMTATTMAVFDGTIINVALPQIS AL TSAA S+WV Sbjct: 10 MELFSDHPNDEGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWV 69 Query: 61 ANAYLLATAMTLAVFAALSSRLGFRTQFTAGLAVFTLASLGCALSRSVEWLIAMRVLQGL 120 +NAYLLATAMTLA FAALSSR+GFR QF+AGLAVFTLASLGCALS SV LI MR+LQGL Sbjct: 70 SNAYLLATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGL 129 Query: 121 GGAAMLSIAPAIHRTVFPNRLLGRILGLNAVLVAAGTAVGPVLGGTLLAALSWEWIFLIN 180 GGAAMLSIAPAIHRT+FPNRLLGRILGLNAVLVAA TAVGPVLGGTLLA L WEWIF IN Sbjct: 130 GGAAMLSIAPAIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFAIN 189 Query: 181 LPLGMVGVLLAWSAIPETRKPARARFDIAGACWSALAMGALIMAADTCARLAEPGQGTQA 240 +PLG++ V LAW+A+P+TRKPA+A FD+AGA WSALAMGALIMAADTCAR AEPGQG+ A Sbjct: 190 VPLGVLAVWLAWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSGA 249 Query: 241 TLQAAAYALVALLAALAFVRGQRRAPEPLLPLDIFANARFSLAALTSLVSFVGQGIAFVA 300 AAAYA+ ALLA LAFVRGQRRA +PLLPLDIFAN RFSLAALTSL SFVGQGIAFVA Sbjct: 250 AFTAAAYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVA 309 Query: 301 LPFLFQNGYGYSAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFTVGLAL 360 LPFLFQN YGY+AFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALF +GL L Sbjct: 310 LPFLFQNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFALGLVL 369 Query: 361 LALLPAHAQAWDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQC 420 LALLP AQ WDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQC Sbjct: 370 LALLPDQAQPWDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQC 429 Query: 421 LGAALLGMILAIYTAHAVEQGSLQMSALEDGEAIRVALWVATAATALATAVSASRLRRQA 480 LGAALLG+ILA+ TA AV QG LQM+ ++DGEAIR+ALW+A AATALATAVSASRLRR+A Sbjct: 430 LGAALLGVILAVRTAAAVAQGELQMNPVQDGEAIRIALWLAAAATALATAVSASRLRRKA 489 Query: 481 QSPA 484 +SPA Sbjct: 490 ESPA 493 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 309 bits (792), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 182/418 (43%), Positives = 260/418 (62%), Gaps = 8/418 (1%) Query: 11 EGLPGQQRRRAMLAVMTATTMAVFDGTIINVALPQISLALHTSAAASVWVANAYLLATAM 70 EGLP +QR RA+LA+ A +A D I N ALP ++L L T+ AASVW+ AY LA + Sbjct: 22 EGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALDLQTTPAASVWIVTAYQLAMMV 81 Query: 71 TLAVFAALSSRLGFRTQFTAGLAVFTLASLGCALSRSVEWLIAMRVLQGLGGAAMLSIAP 130 L AAL +G+R + GL +FT ASL CA++ S+ L+ R LQGLGGA ++S+ Sbjct: 82 ALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLPTLVMARFLQGLGGAGIMSVNT 141 Query: 131 AIHRTVFPNRLLGRILGLNAVLVAAGTAVGPVLGGTLLAALSWEWIFLINLPLGMVGVLL 190 A+ R ++P R LGR +GLN ++VA VGP + +LA W W+F +N+PLG++ V+L Sbjct: 142 ALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGVLAVVL 201 Query: 191 AWSAIPETRKPARARFDIAGACWSALAMGALIMAADTCARLAEPGQGTQATLQAAAYALV 250 A ++P T AR FD+ A +A A G LI+A A A Q + L+ AA Sbjct: 202 AVPSLPHTSL-ARHAFDLRSALLNAAAFGLLILAIGEGAHQA---QASVVGLELAA---- 253 Query: 251 ALLAALAFVRGQRRAPEPLLPLDIFANARFSLAALTSLVSFVGQGIAFVALPFLFQNGYG 310 A+L L ++ + P P+LP+D+F + FSL+A+T++ SF QG+AFV+LPF F + G Sbjct: 254 AILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLAFVSLPFFFHHDLG 313 Query: 311 YSAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFTVGLALLALLPAHAQA 370 S E L TPWP+ + ++AP AGR++DR+ +L +GLAL GL + +PA Sbjct: 314 RSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGMLGGVGLALLCAGLLSMTFMPASPSV 373 Query: 371 WDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQCLGAALLGM 428 W+I WR L+CGMGFGFFQSPN + ++ + RSG ASG++A R GQ +GAAL+ + Sbjct: 374 WEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLLGQSVGAALVAL 431 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 131 bits (329), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 133/450 (29%), Positives = 208/450 (46%), Gaps = 21/450 (4%) Query: 1 MELFSDLPGDEG--LPGQQRRRAMLAVMTATTMAVFDGTIINVALPQISLALHTSAAASV 58 M L +D G LP ++ A L + + D TI+ ALP I L + Sbjct: 1 MALHTDSAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYA- 59 Query: 59 WVANAYLLATAMTLAVFAALSSRLGFRTQFTAGLAVFTLASLGCALSRSVEWLIAMRVLQ 118 WVA AYLL + +T+ +F L G + A + VF AS C ++ S+ +L+ R LQ Sbjct: 60 WVATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQ 119 Query: 119 GLGGAAMLSIAPAIHRTVFPN-RLLGRILGLNAVLVAAGTAVGPVLGGTLLAALSWEWIF 177 G+GG ++ A +FP+ R+ R + +V TAVGP LGG L L W W+F Sbjct: 120 GIGGGMLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVF 179 Query: 178 LINLPLGMVGVLLAWSAIPETRKPARA---RFDIAGACWSALAMGALIMAADTCARLAEP 234 NLP+G++ + W +P R A R D GA +L++G+L + + P Sbjct: 180 YCNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELL-----P 234 Query: 235 GQGTQATLQAAAYALVALLAALAFVRGQRRAPEPLLPLDIFAN-ARFSLAALTSLVSFVG 293 +G + + VA A A + ++RA +P+LP ++ + A SL L+ L F Sbjct: 235 KRGVTSGMLVLLAVSVA--AFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAM 292 Query: 294 QGIAFVALPFLFQNGYGYSAFESAALFTPWPLGIVLVAPHAGRLADR-HSPALLSSLGLA 352 + A P LFQ G+G S E+ L TP I + + GR+ R +P L+ +LG A Sbjct: 293 FSMLMYA-PLLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFA 351 Query: 353 LFTVGLALLALLPAHAQAWDIGWRALVCGMGFGFFQSPNNREMLGN--VSRERSGNASGV 410 L + + L ++ A++ GFG N + +RE G A+ + Sbjct: 352 L--IAASCLGIVLCDRVTGKGFLLAVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATAL 409 Query: 411 LAIMRTFGQCLGAALLGMILAIYTAHAVEQ 440 L +R G LG A+ G +++ VE+ Sbjct: 410 LQSLRMIGGMLGTAITGTLVSQMYGAGVEK 439 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 129 bits (325), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 136/432 (31%), Positives = 196/432 (45%), Gaps = 27/432 (6%) Query: 20 RAMLAVMTATTMAVFDGTIINVALPQISLALHTSAAASVWVANAYLLATAMTLAVFAALS 79 R +L M V D ++ + LP ++ L S + WV ++Y+L A L A + Sbjct: 36 RILLTGSFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATA 95 Query: 80 SRLGFRTQFTAGLAVFTLASLGCALSRSVEWLIAMRVLQGLGGAAMLS-----IAPAIHR 134 R G R G+A+F L SL CAL+ S + L R LQG+G A +L+ I A H Sbjct: 96 DRYGRRRVLLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHD 155 Query: 135 TVFPNR---LLGRILGLNAVLVAAGTAVGPVLGGTLLAALSWEWIFLINLPLGMVGVLLA 191 R L G I+GL VL P++GG + L W W F +NLP ++ + Sbjct: 156 EDQRERAWALWGGIMGLTMVLA-------PLIGGVVNTLLGWRWAFAVNLPACLLLAVAV 208 Query: 192 WSAIPETRKPARARFDIAGACWSALAMGALIMAADTCARLAEPGQGTQATLQAAAYALVA 251 W +PE+R P D G S+ A+ L T A + P G ++ A L Sbjct: 209 WRHVPESRNPLPRPLDPLGILSSSSAVFLL-----TWALITGPEHG-WTSVACVARGLGG 262 Query: 252 LLAALAFVRGQRRAPEPLLPLDIFANARFSLAALTSLVSFVGQGIAFVALPFLFQNGYGY 311 L + F+ QRRA P+L L +F +A F A + LP QN G Sbjct: 263 ALLLVLFIAWQRRAAHPMLELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGL 322 Query: 312 SAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFTVGLALLALLPAHAQAW 371 SA+++ P+ L ++L+ RLA S + +LGL + +G LL + A + A Sbjct: 323 SAWQTGLAMLPFALAMLLLPQVGSRLARWLSSPQMLALGLGVTALG-NLLMIQAALSGA- 380 Query: 372 DIGWRAL---VCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQCLGAALLGM 428 W AL V G G G + ++GNV RER+G ASG+ R G G A LG Sbjct: 381 -AAWTALGMAVLGSGGGLLNGQTQKAIMGNVPRERAGMASGISTTARFTGVLAGFAGLGA 439 Query: 429 ILAIYTAHAVEQ 440 +LA HA++ Sbjct: 440 VLADTARHAMQN 451 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 127 bits (318), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 113/341 (33%), Positives = 177/341 (51%), Gaps = 10/341 (2%) Query: 22 MLAVMTATTMAVFDGTIINVALPQISLALHTSAAASVWVANAYLLATAMTLAVFAALSSR 81 ++A+ MA+ D T++NV L I+ +L + VW+ + Y L A L V AL+ R Sbjct: 32 LVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALADR 91 Query: 82 LGFRTQFTAGLAVFTLASLGCALSRSVEWLIAMRVLQGLGGAAMLSIAPAIHRTVFP-NR 140 G R + GL +F LASL C + + +LI R+LQGLG A + + ++ V+ +R Sbjct: 92 FGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYEDDR 151 Query: 141 LLGRILGLNAVLVAAGTAVGPVLGGTLLAALSWEWIFLINLPLGMVGVLLAWSAIPETRK 200 + R+LG+ + V AVGP++GG L+ L W +FLIN+P+G+VG+++A IP+ Sbjct: 152 VRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQVGG 211 Query: 201 PARARFDIAGACWSALAMGALIMAADTCARLAEPGQGTQATLQAAAYALVALLAALAFVR 260 ARA +++ S LA G +++A + + P G + A A VAL A A +R Sbjct: 212 HARA-LNLS----SHLA-GVVMLAGLSFVLIQGPVYGWTSPRILAGLA-VALSAGAALIR 264 Query: 261 GQRRAPEPLLPLDIFANARFSLA-ALTSLVSFVGQGIAFVALPFLFQNGYGYSAFESAAL 319 + R PLLP +FA +F+ A + L++F G F L Q G G A ++ Sbjct: 265 HELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFY-LALFLQQGRGADALQTGLQ 323 Query: 320 FTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFTVGLAL 360 P I +GR++ P + LGL++ TV AL Sbjct: 324 LVPMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTVFTAL 364 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,299,795 Number of extensions: 50695 Number of successful extensions: 427 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 402 Number of HSP's successfully gapped: 16 Length of query: 484 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 395 Effective length of database: 1,198,455 Effective search space: 473389725 Effective search space used: 473389725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)