BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0513.p (620 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 1206 0.0 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 246 4e-66 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 226 3e-60 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 194 2e-50 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 179 8e-46 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 1206 bits (3119), Expect = 0.0, Method: Compositional matrix adjust. Identities = 580/620 (93%), Positives = 603/620 (97%) Query: 1 MPTYRSHTTTQGRNMAGARALWRATGMKDGDFNKPIIAVVNSFTQFVPGHVHLKDLGQLV 60 MPTYRS+TTTQGRNMAGARALWRATGMKDGDF KPIIAVVNSFTQFVPGHVHLKDLGQLV Sbjct: 1 MPTYRSYTTTQGRNMAGARALWRATGMKDGDFTKPIIAVVNSFTQFVPGHVHLKDLGQLV 60 Query: 61 AREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDLIADSVEYMVNAHCADAMVCIS 120 AREIEAAGGVAKEFNTIAVDD G MLYSLPSRDLIADSVEYMVNAHCADAMVCIS Sbjct: 61 AREIEAAGGVAKEFNTIAVDDSGTHGDARMLYSLPSRDLIADSVEYMVNAHCADAMVCIS 120 Query: 121 NCDKITPGMLMAAMRLNIPVIFVSGGPMEAGKVIKTVNTDKKIIKLDLVDAMIQAGDQNI 180 NCDKITPGMLMAAMRLNIPV+FVSGGPMEAGKVIKTVNTDKK+IKLDLVDAMIQAGD+NI Sbjct: 121 NCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVIKTVNTDKKVIKLDLVDAMIQAGDKNI 180 Query: 181 SDADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAG 240 SDADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAG Sbjct: 181 SDADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAG 240 Query: 241 RLIVELAKRHYEEDDYTILPRNIATKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEF 300 RLIVELAKRHYE+DDY+ILPRNI TKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEF Sbjct: 241 RLIVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEF 300 Query: 301 TMADIDRISRKVPCLCKVAPMTNKYHIEDVHRAGGIIAILGELARAGLLDTSRPTVHSKT 360 TMADIDRISR+VPCLCKVAPMTNKYHIEDVHRAGGII+ILGELARAGLLDTSRPTVHSKT Sbjct: 301 TMADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKT 360 Query: 361 LGEAIEKYDIRVTSDAEVHKLFRAAPGGVPTQVAFSQQERFADSDLDRANGCIRDREHAY 420 LGEAIEK+DIRV+SD VHKLFRAAPGGVPTQVAFSQ+ERF +DLDRANGCIRDREHAY Sbjct: 361 LGEAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAY 420 Query: 421 SQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGDQVKAGDVV 480 SQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGD+VKAGDVV Sbjct: 421 SQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGDKVKAGDVV 480 Query: 481 IVRYEGPKGGPGMQEMLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGG 540 IVRYEGPKGGPGMQEMLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGG Sbjct: 481 IVRYEGPKGGPGMQEMLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGG 540 Query: 541 AIGLVQEGDLIDIDIPNRSINLRVTAEELAQRRAAMEALGDAAWKPANRTRVVSQALQAY 600 AIGLV+EGD+IDIDIPNR+INLR+++++LA+RR AMEA GDAAW+P NR RVVSQALQAY Sbjct: 541 AIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARGDAAWQPGNRQRVVSQALQAY 600 Query: 601 AALTTSADRGAVRDLSQLKR 620 AALTTSADRGAVRDLSQLKR Sbjct: 601 AALTTSADRGAVRDLSQLKR 620 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 246 bits (628), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 183/612 (29%), Positives = 311/612 (50%), Gaps = 73/612 (11%) Query: 11 QGRNMAGARALWRATGMKDGDFN-KPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGG 69 Q R+ R+ + G+ F+ +P+I + N++++ P + H + L + V I AGG Sbjct: 19 QDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFRALAEQVKIGIWEAGG 78 Query: 70 VAKEFNTIAVDDGIAMGHDGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGM 129 EF +++ + + + MLY R+L + VE + A+ D +V + CDK TP + Sbjct: 79 FPLEFPVMSLGETL-LRPTAMLY----RNLASMDVEESIRANPLDGVVLLMGCDKTTPAL 133 Query: 130 LMAAMRLNIPVIFVSGGPMEAGKVI-KTVNTDKKIIKLDLVDAMIQAGDQNISDADVAEV 188 LM A +++P I VSGGPM +GK + + + + ++ ++AG ++ + E Sbjct: 134 LMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQM---SEDVRAG--KMTQEEFFEA 188 Query: 189 ERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAK 248 E + G C M TA++M + E LG+ LP N I A A R L AGR IV++ Sbjct: 189 ESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMV- 247 Query: 249 RHYEEDDYTILPRNIATKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEFTMADIDRI 308 EED ++ I T++ +ENA+ ++ ++GGSTN V+HLLA A ++ + D D I Sbjct: 248 ---EED---LVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDDI 301 Query: 309 SRKVPCLCKVAPMTNKYHIEDVHRAGGIIAILGELARAGLLDTSRPTVHSKTLGEAIEKY 368 +++PCL + P + K+ +ED + AGG+ A++ +L ++D + T + KTL E + Sbjct: 302 GQQLPCLLDLQP-SGKFLMEDFYYAGGLPAVIRQL--ESVIDKTALTANGKTLWENCQD- 357 Query: 369 DIRVTSDAEVHKLFRAAPGGVPTQVAFSQQERFADSDLDRANGCIRDREHAYSQDGGLAV 428 AP ++++ IR + + + G+A+ Sbjct: 358 ----------------APN-------WNEE-------------VIRSFDKPFKEAAGIAI 381 Query: 429 LYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGDQVKAGD--VVIVRYEG 486 L GN+A G ++K + ++LK G A VFE+ D + I + + + V++++ G Sbjct: 382 LKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCG 441 Query: 487 PKGGPGMQE---MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIG 543 PKG PGM E M P ++ KG+ + +D R SG + G V+ H SPEAA GG + Sbjct: 442 PKGYPGMAEAGNMPLPPKILR-KGITDMVRV-SDARMSGTAYGTVVLHVSPEAAAGGPLA 499 Query: 544 LVQEGDLIDIDIPNRSINLRVTAEELAQRRAAMEALGDAAWKPANRTRVVSQALQAYAAL 603 +VQ GD I++D+ R ++L V+ EELA+RRA + KP ++ ++ Y Sbjct: 500 VVQNGDFIELDVEARKLHLDVSDEELARRRAQWQ-------KPELPPQMQRGWVKLYVDH 552 Query: 604 TTSADRGAVRDL 615 A++GA D Sbjct: 553 VQQANQGADLDF 564 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 226 bits (577), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 176/576 (30%), Positives = 286/576 (49%), Gaps = 69/576 (11%) Query: 10 TQGRNMAGARALWRATGMKDGDFN-KPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAG 68 T +N R+ + G+ D +F KP+I + N++++ P + H + + + V R I AG Sbjct: 20 TADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRKIAEHVRRGIIEAG 79 Query: 69 GVAKEFNTIAVDDGIAMGHDGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPG 128 G EF + + + ML +R+L + VE + + DA+V ++ CDK TP Sbjct: 80 GFPVEFPVFSNGES-NLRPTAML----TRNLASMDVEESIRGNPIDAVVLLTGCDKTTPA 134 Query: 129 MLMAAMRLNIPVIFVSGGPMEAGKVI-KTVNTDKKIIKLDLVDAMIQAGDQNISDADVAE 187 +LM A ++P I V+GGPM GK + + + + +L ++AG+ I D AE Sbjct: 135 LLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQL---SEQVKAGEITIHDFMAAE 191 Query: 188 --VERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAGRLIVE 245 + RSA G+C+ M TA++M C+ E LG+SLP N I A A R L +G IV+ Sbjct: 192 AGMSRSA----GTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIVD 247 Query: 246 LAKRHYEEDDYTILPRNIATKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEFTMADI 305 + +E + I T++ +ENA+ + ++GGSTN V+HL A A V+ + D Sbjct: 248 MV---WE----GLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDW 300 Query: 306 DRISRKVPCLCKVAPMTNKYHIEDVHRAGGIIAILGELARAGLLDTSRP-TVHSKTLGEA 364 RI R P + + P + +Y +E+ + AGG+ A+L L A LL TV+ +T+ + Sbjct: 301 TRIGRGTPTIVDLQP-SGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDN 359 Query: 365 IEKYDIRVTSDAEVHKLFRAAPGGVPTQVAFSQQERFADSDLDRANGCIRDREHAYSQDG 424 ++ DA ++ + +R +DG Sbjct: 360 VK--------DAPIYN-----------------------------DEVVRPLAKPLIEDG 382 Query: 425 GLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGD--QVKAGDVVIV 482 G+ +L GN+A +G ++K + ++K G A VFE + E I V A V+++ Sbjct: 383 GICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDASCVLVM 442 Query: 483 RYEGPKGGPGMQE---MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEG 539 + GPKG PGM E M P + + G+ K +D R SG + G VI H +PEAA G Sbjct: 443 KNVGPKGYPGMAEVGNMGLPPKVLAT-GV-KDMVRISDARMSGTAYGTVILHVAPEAAAG 500 Query: 540 GAIGLVQEGDLIDIDIPNRSINLRVTAEELAQRRAA 575 G +G+VQ+GD I++D + L ++ EE+ +R A Sbjct: 501 GPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEA 536 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 194 bits (493), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 167/558 (29%), Positives = 267/558 (47%), Gaps = 75/558 (13%) Query: 34 KPIIAVVNSFTQFVP-GHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLY 92 +PII + S + P +HL +L + V I AGG+ EF + + + Sbjct: 47 RPIIGIAQSGSDISPCNRIHL-ELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAI-- 103 Query: 93 SLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVIFVSGGPMEAGK 152 R+L + +++ + DA+V + CDK TP +MAA ++IP I +SGGPM G Sbjct: 104 ---DRNLAYLGLVEILHGYPIDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGW 160 Query: 153 VI-KTVNTDKKIIKLDLVDAMIQAGDQNISDADVAEVERSACPTCGSCSGMFTANSMNCL 211 + + V + I K ++ AG +I + E+ ++ P+ G C+ M TA++MN + Sbjct: 161 MDGELVGSGSAIWK---GRKLLSAG--SIDNEKFLEIAAASAPSSGHCNTMGTASTMNAM 215 Query: 212 TEVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAKRHYEEDDYTILPRNIATKETWEN 271 E LG+SL G I A + +R ++ GR IV +A YE+ + P I T++ + + Sbjct: 216 AEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMA---YED----LRPSAILTRDAFLD 268 Query: 272 AMTLDVSMGGSTNTVLHLLAAAHEAKVEFTMADIDRISRKVPCLCKVAPMTNKYHIEDVH 331 A+ ++ ++GGSTN H++A A A VE D + VP L + P KY E H Sbjct: 269 AIVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYDVPLLLNMQP-AGKYLGERFH 327 Query: 332 RAGGIIAILGELARAGLLDTSRPTVHSKTLGEAIEKYDIRVTSDAEVHKLFRAAPGGVPT 391 RAGG+ AI+ EL +AG L R T KT+ E ++ R ++D E+ F AAP Sbjct: 328 RAGGVPAIMWELQQAGKLRAERITATGKTMAENLQG---RASNDREMIYPF-AAP----- 378 Query: 392 QVAFSQQERFADSDLDRANGCIRDREHAYSQDGGLAVLYGNIAEKGCIVKTAGVDESI-- 449 +R+R G VL GN+ + I+KT+ + E+ Sbjct: 379 ---------------------LRER-------AGFLVLKGNLFDF-AIMKTSVISETFRE 409 Query: 450 --LKFSGTARVFESQDAAVEGILGDQVKAGD---------VVIVRYEGPKGGPGMQEM-- 496 L G +FE + +G + D ++ +R GP G PG E+ Sbjct: 410 RYLSTPGQENIFECRAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVN 469 Query: 497 LYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVQEGDLIDIDIP 556 + P + +G+ L DGR SG S I +ASPE+A GG + +++GD + ID+ Sbjct: 470 MQPPDALIKRGVSTLPTL-GDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLN 528 Query: 557 NRSINLRVTAEELAQRRA 574 N+ V+ EELA+R++ Sbjct: 529 TGECNMLVSEEELARRKS 546 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 179 bits (453), Expect = 8e-46, Method: Compositional matrix adjust. Identities = 160/543 (29%), Positives = 249/543 (45%), Gaps = 41/543 (7%) Query: 35 PIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAK-EFNTIAVDDGIAMGHDGMLYS 93 P +A+V+++ + H + Q++ AG VA+ + A+ DG+ G GM S Sbjct: 68 PSLAIVSAYNDMLSAHQPFERFPQIIKDAAREAGAVAQFAGGSPAMCDGVTQGQAGMELS 127 Query: 94 LPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRL-NIPVIFVSGGPMEAGK 152 L SRD IA + ++ + DA + + CDKI PG+L+ A+ ++P +FV GPM +G Sbjct: 128 LFSRDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMTSG- 186 Query: 153 VIKTVNTDKKIIKLDLVDAMIQAGDQNISDADVAEVERSACPTCGSCSGMFTANSMNCLT 212 N+DK I+ + AG I A++ E E + G+C+ TANS L Sbjct: 187 ---MSNSDKVKIR-----QLYTAG--KIGRAELLEAESKSYHGAGTCTFYGTANSNQMLM 236 Query: 213 EVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAKRHYEEDDYTILPRNIATKETWENA 272 E +GL LPG I R L A + ++ + YT + R I K + NA Sbjct: 237 EAMGLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGTQ---YTPVGRMIDEK-SIVNA 292 Query: 273 MTLDVSMGGSTNTVLHLLAAAHEAKVEFTMADIDRISRKVPCLCKVAPMTNKYHIEDVHR 332 + + GGSTN LHL+A A A + D D++S VP + K+ P + H Sbjct: 293 IVALHATGGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYP-NGTADVNHFHA 351 Query: 333 AGGIIAILGELARAGLLDTSRPTVHSKTLGEAIEKYDIRVTSDAEVHKLFRAAPGGVPTQ 392 AGG +L EL AGL+ T+ LG+ + ++ AE PG V Sbjct: 352 AGGTGFVLRELIDAGLMHDDVTTI----LGQGLRQHCKEPMLAAEGEN---GRPGIVTWH 404 Query: 393 VAFSQQERFADSDLDRANGCIRDREHAYSQDGGLAVLYGNIAEKGCIVKTAGVDESILKF 452 A +Q G + ++ DGGL +L GN+ ++K + V Sbjct: 405 DAPAQSGD---------EGVLGTVAKPFAADGGLKLLQGNLGR--AVIKISAVKPEHRVV 453 Query: 453 SGTARVFESQDAAVEGILGDQVKAGDVVIVRYEGPK--GGPGMQEMLYPTSYIKSKGLGK 510 A VF SQ+A + Q+ V ++ ++GP+ G P + + ++ K G+ Sbjct: 454 EAPAVVFHSQEAFMAAFKAGQLDRDFVAVITFQGPRANGMPELHALTPALGILQDK--GR 511 Query: 511 ACALFTDGRFSGGSSGLVIG-HASPEAAEGGAIGLVQEGDLIDIDIPNRSINLRVTAEEL 569 AL TDGR SG S + H +PE GG +G V++GD+I +D ++ +V A E Sbjct: 512 HVALVTDGRMSGASGKVPAAIHVTPEVLGGGPLGRVRDGDIIRLDAEAGTLEAKVDATEW 571 Query: 570 AQR 572 A R Sbjct: 572 AAR 574 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,972,910 Number of extensions: 85029 Number of successful extensions: 321 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 297 Number of HSP's successfully gapped: 8 Length of query: 620 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 529 Effective length of database: 1,188,985 Effective search space: 628973065 Effective search space used: 628973065 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)