BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0500.p (522 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase... 902 0.0 gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase... 189 4e-49 >gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 525 Score = 902 bits (2332), Expect = 0.0, Method: Compositional matrix adjust. Identities = 455/525 (86%), Positives = 487/525 (92%), Gaps = 3/525 (0%) Query: 1 LNNWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPAIQTACELGFGQGLSTNFHAA 60 +NNWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPA+QTACELGFGQGLS+N HAA Sbjct: 1 MNNWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPAVQTACELGFGQGLSSNIHAA 60 Query: 61 ASTTRWWGNDFNPMQAAFAQELAAASGSGAQLEDSSFSHFCRRDDLPDFDFIALHGIWSW 120 ASTTRWWGNDFNP QAAFAQELAAASGSGAQLEDSSFS FCRRDDLPDFDFIALHGIWSW Sbjct: 61 ASTTRWWGNDFNPRQAAFAQELAAASGSGAQLEDSSFSRFCRRDDLPDFDFIALHGIWSW 120 Query: 121 VSQDNRDILFDFIDRKLKPGGVLYLSYNTQPGWSAFLPLRELLVQHTQLAGNQEQGLPTS 180 VSQ+NRDI+FDFIDRKLKPGGVLY+SYNTQPGWSAF+PLRELLVQHTQ +GN+EQGLPT+ Sbjct: 121 VSQENRDIIFDFIDRKLKPGGVLYISYNTQPGWSAFMPLRELLVQHTQQSGNEEQGLPTA 180 Query: 181 ARIEAALAFAARLFETDPVYAQANPFMRDRLKILQQQPVSYLAHEYFNRDWHAETFAEIH 240 ARI+AALAFAARLFE DPVYAQANPFMRDRLK+LQQQ SYLAHEYFNRDWH E+FA++H Sbjct: 181 ARIDAALAFAARLFEADPVYAQANPFMRDRLKVLQQQSRSYLAHEYFNRDWHVESFADMH 240 Query: 241 RRFSPAGLQFACPAHYIDHLDMANLTPAQRQCLSVIDDPVLYQSTRDFMVNQTFRRDYWV 300 RRFS AGL+FACPAHY+DHLDMANLTPAQRQ L I DPVL QS RDFMVNQ FRRDYWV Sbjct: 241 RRFSGAGLEFACPAHYVDHLDMANLTPAQRQQLYEITDPVLQQSVRDFMVNQQFRRDYWV 300 Query: 301 RGGQLLDERQRVQALALQSVVLLTEPDKVPLTIQNVASEITLNRLIYEPILQALSDYQPH 360 RG Q LD+RQRVQALALQSV+LLTEPDKVPLTIQNVASEITLNRLIYEPILQALSDYQPH Sbjct: 301 RGAQALDDRQRVQALALQSVILLTEPDKVPLTIQNVASEITLNRLIYEPILQALSDYQPH 360 Query: 361 TLVDLAASLAHRNLNLSQVIDSALMLIGTGHAAPLPAEVDAATQAQIARRTADLNAYLLQ 420 TLVDLAASLAHRNLNL+QV+DS LMLIGTGHAAPLPAE DAAT+AQI RRT DLNA+LLQ Sbjct: 361 TLVDLAASLAHRNLNLAQVLDSVLMLIGTGHAAPLPAEPDAATRAQIERRTGDLNAWLLQ 420 Query: 421 RATLVLP---DSQEGEISHLVSPVTGGGVKVDQVEQLLLLARSVCGDDVDAWPAEVWRHI 477 RA L P +EGEI+HL SPV GGGV+VD+VEQL LLAR CGDDVDAWPA+VWR+I Sbjct: 421 RAALASPAGEQGEEGEITHLSSPVIGGGVRVDRVEQLFLLARQSCGDDVDAWPAQVWRYI 480 Query: 478 SAQGKRLVREGVRLEGEQENLAELAAQARLFARTRLPLLRALQVV 522 +AQGKRL+REGVRLEGE ENLAELA QARLFARTRLPLL+AL VV Sbjct: 481 AAQGKRLLREGVRLEGEAENLAELATQARLFARTRLPLLKALGVV 525 >gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 523 Score = 189 bits (480), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 153/524 (29%), Positives = 244/524 (46%), Gaps = 28/524 (5%) Query: 3 NWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPAIQ---TACELGFGQGLSTNFHA 59 W+ GYV I Y G++P +P L A G++ + T ELG GQGL++ A Sbjct: 2 QWSSGYVHGIDYPVGFFPEQSPVHLSFACLAQGVEPVPLDREFTYMELGCGQGLTSTVLA 61 Query: 60 AAS-TTRWWGNDFNPMQAAFAQELAAASG-SGAQLEDSSFSHFCRRD-DLPDFDFIALHG 116 AA+ R++ DF P Q AQ++A+A+G + + SF R + DLP FDF+ ++G Sbjct: 62 AANPQGRFYAVDFLPTQVRTAQDMASAAGIDNLTVLECSFEQMARGEIDLPAFDFVTMYG 121 Query: 117 IWSWVSQDNRDILFDFIDRKLKPGGVLYLSYNTQPGWSAFLPLRELLVQHTQ--LAGNQE 174 ++SWV + NR + DF+ R LKPGG++YL+YN PGWS+ L L+ +L + + + Q Sbjct: 122 VYSWVDRTNRGHILDFLRRYLKPGGIVYLNYNALPGWSSALSLQRMLREFGRHDMCDRQA 181 Query: 175 QGLPTSARIEAALAFAARLFETDPVYAQANPFMRDRLKILQQQPVSYLAHEYFNRDWHAE 234 Q AL A A +R RL+ L+ YLAHEY N+ W A Sbjct: 182 Q-----LHQGGALVRALSQAGAAAFGAAEATGLRRRLRALESDEPGYLAHEYLNQGWDAL 236 Query: 235 TFAEIHRRFSPAGLQFACPAHYIDHLDMANLTPAQRQCLSVIDDPVLYQSTRDFMVNQTF 294 ++ + + A L + A L+ AQ+ L+ + + + D + N +F Sbjct: 237 FSIDVIQDLAQAKLDYVGSAELAYGFPELYLSHAQQSLLAQQPNAGMRELVWDQICNSSF 296 Query: 295 RRDYWVRGGQLLDERQRVQALALQSVVLLTEPDKVPLTIQNVASEITLNRLIYEPILQAL 354 R D +VRG + + QR L + L P ++ L + + + Y+P++ AL Sbjct: 297 REDIYVRGARRMSPHQRDAWLERCGLALTEAPSRITLELPLAIGNVRADAHTYQPVMDAL 356 Query: 355 SDYQPHTLVDLAA--SLAHRNLNLSQVIDSALMLIGTGHAAPLPAEVDAATQAQIARRTA 412 +PH+ +LAA +L + L ++ A++L + AAP + AQ + Sbjct: 357 CQ-RPHSFAELAALPALRKQGRGLREIASMAILLSASRQAAPF----FLSAAAQPRQAAQ 411 Query: 413 DLNAYLLQRATLVLPDSQEGE-ISHLVSPVTGGGVKVDQVEQLLLLARSVCGDDVDAWPA 471 LN L+ S E + L SP+ G G+ ++++ S + W Sbjct: 412 RLN-------RLIAGWSAESDCFQALASPLLGNGLPSGLLQRVAFDQLSQAQSQPEQWVL 464 Query: 472 EVWRHISAQGKRLVREGVRLEGEQENLAELAAQARLFARTRLPL 515 + + I QG L R G L +E AEL A A+ RLP+ Sbjct: 465 PMAQAIHRQGTHLQRGGAPLSTREEIHAELLALAQAICTQRLPV 508 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,559,707 Number of extensions: 62088 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 176 Number of HSP's successfully gapped: 2 Length of query: 522 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 432 Effective length of database: 1,193,720 Effective search space: 515687040 Effective search space used: 515687040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)