BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0493.p (70 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309840|ref|YP_003773932.1| cold-shock transcription regula... 140 2e-35 gi|300312332|ref|YP_003776424.1| cold shock-like transcription r... 102 6e-24 gi|300310729|ref|YP_003774821.1| cold-shock DNA-binding domain-c... 93 5e-21 >gi|300309840|ref|YP_003773932.1| cold-shock transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 70 Score = 140 bits (352), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 68/70 (97%), Positives = 69/70 (98%) Query: 1 MSTQTGVVKWFNDAKGFGFITPDAGGADVFAHFQDIQSNGFRSLSENQRVSFDRGVGPKG 60 MS+QTGVVKWFNDAKGFGFITPDAGGADVFAHFQDIQS GFRSLSENQRVSFDRGVGPKG Sbjct: 1 MSSQTGVVKWFNDAKGFGFITPDAGGADVFAHFQDIQSTGFRSLSENQRVSFDRGVGPKG 60 Query: 61 EKATNIKVIA 70 EKATNIKVIA Sbjct: 61 EKATNIKVIA 70 >gi|300312332|ref|YP_003776424.1| cold shock-like transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 68 Score = 102 bits (254), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 47/62 (75%), Positives = 53/62 (85%) Query: 5 TGVVKWFNDAKGFGFITPDAGGADVFAHFQDIQSNGFRSLSENQRVSFDRGVGPKGEKAT 64 TG VKWFNDAKGFGFITPD GG D+FAHF IQSNGF+SL ENQRVSF+ GPKG++A+ Sbjct: 3 TGTVKWFNDAKGFGFITPDEGGEDLFAHFSAIQSNGFKSLKENQRVSFEVTTGPKGKQAS 62 Query: 65 NI 66 NI Sbjct: 63 NI 64 >gi|300310729|ref|YP_003774821.1| cold-shock DNA-binding domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 68 Score = 92.8 bits (229), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 43/66 (65%), Positives = 52/66 (78%) Query: 5 TGVVKWFNDAKGFGFITPDAGGADVFAHFQDIQSNGFRSLSENQRVSFDRGVGPKGEKAT 64 TG VKWFND+KGFGFITPD GG D+FAHF IQ NGF++L E Q+V FD GPKG++A+ Sbjct: 3 TGTVKWFNDSKGFGFITPDDGGEDLFAHFSAIQMNGFKTLKEGQKVQFDVTQGPKGKQAS 62 Query: 65 NIKVIA 70 NI+ A Sbjct: 63 NIQNAA 68 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 222,946 Number of extensions: 7039 Number of successful extensions: 20 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 20 Number of HSP's successfully gapped: 3 Length of query: 70 Length of database: 1,619,870 Length adjustment: 42 Effective length of query: 28 Effective length of database: 1,421,000 Effective search space: 39788000 Effective search space used: 39788000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)