BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0492.p (955 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309839|ref|YP_003773931.1| metal-dependent phosphohydrolas... 1529 0.0 gi|300309652|ref|YP_003773744.1| HD-GYP domain-containing protei... 271 2e-73 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 72 3e-13 gi|300310565|ref|YP_003774657.1| response regulator containing a... 70 1e-12 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 68 3e-12 >gi|300309839|ref|YP_003773931.1| metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 975 Score = 1529 bits (3958), Expect = 0.0, Method: Compositional matrix adjust. Identities = 768/947 (81%), Positives = 835/947 (88%), Gaps = 4/947 (0%) Query: 1 LPVRFGISGLVMAGMILLAVVLVSVGWWGARQVLLDMAERSAEDAGRIMTERSRRIIDPA 60 LPVRFGIS LVMAGMILLAVVLV+VGWWGARQVLLDMAERSA+DAGRIMTERSRRIIDPA Sbjct: 27 LPVRFGISALVMAGMILLAVVLVTVGWWGARQVLLDMAERSAQDAGRIMTERSRRIIDPA 86 Query: 61 VATLRQLSFDPITQATDLDERLTRIAAFSIDLVANPLVSAVYVGYPNGDFLLVRSLRGAE 120 VATLRQL+FDPITQA +LD RL RIAAFS DLVANPLVSAVYVGYPNGDFLLVR+LRGAE Sbjct: 87 VATLRQLAFDPITQAGELDTRLARIAAFSADLVANPLVSAVYVGYPNGDFLLVRALRGAE 146 Query: 121 QRAFFVAPPEATFLVQSIVRDAAGQQHGQYRFYNAELKLLALQEQPQYRFDPRTRPWYAR 180 QR+FF AP A FLVQ+I R+A GQ+HGQYRFY+ +L+LL+L+EQP+Y FDPRTRPWYA Sbjct: 147 QRSFFRAPESARFLVQTITRNANGQEHGQYRFYDDDLRLLSLREQPEYHFDPRTRPWYAS 206 Query: 181 ADRSLTPAISAPYVFFTTRQIGISLSQRARNSDAVVALDMTLDDLAVSLGNLRLTPHSEL 240 A+R+L P+ISAPYVFFTTRQIGISLSQRA S+AVVA+DMTLDDLA SLGNLRLTPHSEL Sbjct: 207 ANRALMPSISAPYVFFTTRQIGISLSQRAHASEAVVAIDMTLDDLATSLGNLRLTPHSEL 266 Query: 241 ALVDDADKVIAYSDMGRVLEQEGDNFRFRGLQDLDVPSLSLLAQRSKAGDTRALYQVEGR 300 ALVD+A KVIAYSDM RVLEQ+GD+FRFR L +L+ P+LSLLA+R+++G T ALY G+ Sbjct: 267 ALVDEAGKVIAYSDMNRVLEQDGDSFRFRSLDELNFPTLSLLARRARSGQTNALYHAGGQ 326 Query: 301 EWLGLSLPFNVIDGHALKLLITAPGDELLGQLDRNRKTMVLVAFLLVLAFLPLGWWAGRA 360 EWLGLSLPFNVID H L LLI APGDELLGQL+RNRKTMVL+A LV+AFLPLGWWAGRA Sbjct: 327 EWLGLSLPFNVIDDHPLHLLIAAPGDELLGQLERNRKTMVLIALFLVIAFLPLGWWAGRA 386 Query: 361 IGLSLERLAQKAARLSRFDFRTQGRGRSTLREVNMLTEMVDHVSLTVEAFLDISAILSTE 420 IG S+ERLA +A R+SRFDFR QG+ S LREVNMLTEMVD+VSLTVEAFLDISAILSTE Sbjct: 387 IGRSMERLAARARRMSRFDFREQGKTASMLREVNMLTEMVDNVSLTVEAFLDISAILSTE 446 Query: 421 THVEQMLQQVLEKFVQATRCDGGAVYLWQREGQQMQRAAAYGQQCGLTDGMSYDEHQAAA 480 THVEQMLQQVLEKF+QATRC GAVYLWQRE QMQRAA YG GL + ++D + Sbjct: 447 THVEQMLQQVLEKFIQATRCQAGAVYLWQRESGQMQRAAGYGADHGLAESFAHDRQHTPS 506 Query: 481 GDAHETEDGRLRVELDLRGRNGSLEGLLVLMHAHDSAHEDPRFLAFAGRLSGMLAVAIET 540 + DG LRVELDLRGRNGSLEGLLVL+HAHDSAH DPRFLAFA RLSGMLAVAIET Sbjct: 507 SNTRALPDGSLRVELDLRGRNGSLEGLLVLVHAHDSAHGDPRFLAFARRLSGMLAVAIET 566 Query: 541 RQLIAAQKALLESLIRLMADAIDAKSPYTGGHCERVPHLAIMLVERLAQEREGRYADFQL 600 RQLIAAQKALLESLIRLMADAIDAKSPYTGGHCERVPHLAIMLVERLAQEREG YA F L Sbjct: 567 RQLIAAQKALLESLIRLMADAIDAKSPYTGGHCERVPHLAIMLVERLAQEREGPYAGFSL 626 Query: 601 NEDQRYAFQLAALLHDCGKVTSPEHIVDKATKLEVIHNRIHEVRMRFEVLWRDAELELLR 660 NED+RYAF+LAALLHDCGKVTSPEHIVDKATKLEVIHNRIHEVRMRFEVLWRDAEL LL Sbjct: 627 NEDERYAFRLAALLHDCGKVTSPEHIVDKATKLEVIHNRIHEVRMRFEVLWRDAELALLH 686 Query: 661 DYFPALPPAQAEALRRRLDQRRAQLREDFAFVAQCNVGGETMSDEAITRLHQIARQTWQR 720 AL QA+A L+ +RA+LREDFAFVAQCNVG E MS+EAI RL +IA QTW+R Sbjct: 687 QLPQALSEQQAQA----LEAQRARLREDFAFVAQCNVGSEAMSEEAIARLARIATQTWER 742 Query: 721 HFDDSLGLAGEEARRLAASRDGRPSPSLPHTEQLLADRPEHKVAWDDGHRPAVERGDPRN 780 HFDDSLGLA EEARRLAA+R G+ + LP TE LLADRPEH V WD+ HRPAVERGDPRN Sbjct: 743 HFDDSLGLAAEEARRLAATRQGQAAQVLPVTEPLLADRPEHIVPWDEEHRPAVERGDPRN 802 Query: 781 TLGFDMKLPHYRQNMGELHNLTVRRGTLTEEDRFAINNHIVQTLVMLKSLPWPDSLKSVP 840 TLGFDM LP YRQNMGELHNL +RRGTLT EDRFAINNHIVQTLVMLKSLPWP+SLKSVP Sbjct: 803 TLGFDMTLPRYRQNMGELHNLGIRRGTLTPEDRFAINNHIVQTLVMLKSLPWPESLKSVP 862 Query: 841 DTAANHHEKMDGSGYPRRLRASTLPLQDRVMALADVFEALTAADRPYKPAKTLSESLRIM 900 DTAANHHEKMDG+GYPRRL+AS LPLQDRVMALADVFEALTAADRPYKPAKTLSESLRIM Sbjct: 863 DTAANHHEKMDGTGYPRRLKASELPLQDRVMALADVFEALTAADRPYKPAKTLSESLRIM 922 Query: 901 AAMCRDQHLDTELFRYFLRSRLWQAYADELLRPAQRDEVDIDAIEAL 947 A MCR++HLDTELFRYFLRSRLW+ YA ELL+ Q D VDI IEAL Sbjct: 923 ANMCRERHLDTELFRYFLRSRLWEVYAGELLKEGQIDAVDIVEIEAL 969 >gi|300309652|ref|YP_003773744.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 552 Score = 271 bits (694), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 173/449 (38%), Positives = 240/449 (53%), Gaps = 68/449 (15%) Query: 502 GSLEGLLVLMHAHDSAHEDPRFLA-----FAGRLSGMLAVAIETRQLIAAQKALLESLIR 556 G L G+L L++A D A P + F L+ A+A+ +QLI + L ESLIR Sbjct: 161 GELVGVLQLINAIDPATGAPLPFSETDQHFIEALASQAAMAMTNQQLIQRLEQLFESLIR 220 Query: 557 LMADAIDAKSPYTGGHCERVPHLAIMLVERLAQEREGRYADFQLNEDQRYAFQLAALLHD 616 L+ I KSP+TG HCE+VP LA+ML E EG ADF ++E R LAALLHD Sbjct: 221 LINIGIGEKSPHTGRHCEQVPELAMMLAEAAHNATEGPLADFHMSEADRRELWLAALLHD 280 Query: 617 CGKVTSPEHIVDKATKLEVIHNRIHEVRMRFEVLWRDAELELLRDYFPALPPAQAEALRR 676 CGK+++P HIV+KATKLE I +RIH V R EVL RDAE+ +L++ AL Sbjct: 281 CGKISTPTHIVEKATKLETIFDRIHLVETRIEVLKRDAEIRVLQEKL---------ALGA 331 Query: 677 RLDQRRAQLREDFAFVAQCNVGGETMSDEAITRLHQIARQTWQRHFDDSLGLAGEEARRL 736 LD +RA A+ + G ++A+ +R F + + E R Sbjct: 332 ALDAQRA---------AELDAGLAA----------ELAQLDLERAFLRKINVGTEGMR-- 370 Query: 737 AASRDGRPSPSLPHTEQLLADRPEHKVAWDDGH-RPAVERGDPRNTLGFDMKLPHYRQNM 795 P +Q + D + DG RP ++ Sbjct: 371 ------------PEDQQRVLDIAQRSWRGPDGQLRPLLDEE------------------- 399 Query: 796 GELHNLTVRRGTLTEEDRFAINNHIVQTLVMLKSLPWPDSLKSVPDTAANHHEKMDGSGY 855 E NL +R GTL + +R INNHIV T+ ML+SLPWP L V + A HHE+MDG GY Sbjct: 400 -ESANLRIRAGTLNDAERQIINNHIVITIKMLESLPWPKQLARVTEFAGGHHERMDGKGY 458 Query: 856 PRRLRASTLPLQDRVMALADVFEALTAADRPYKPAKTLSESLRIMAAMCRDQHLDTELFR 915 P+ L + +Q R+MA+AD+FEA+TAADRPYK TL+E++ I+A H+D +LF Sbjct: 459 PKGLTREQMSVQARIMAVADIFEAVTAADRPYKRGNTLNEAVEILAGFKARDHIDPDLFD 518 Query: 916 YFLRSRLWQAYADELLRPAQRDEVDIDAI 944 F+R R+++ YA++ + P+Q DEVD+ I Sbjct: 519 LFVRERIYEKYAEKFMHPSQIDEVDLSRI 547 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 71.6 bits (174), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 45/123 (36%), Positives = 67/123 (54%), Gaps = 7/123 (5%) Query: 799 HNLTVRRGTLTEEDRFAINNHIVQTLVMLKSLP-WPDSLKSVPDTAANHHEKMDGSGYPR 857 H L + G L++E+ I H L P PD + D +HHEK+DG+GYP Sbjct: 203 HELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDYAR---DVCLHHHEKIDGNGYPE 259 Query: 858 RLRASTLPLQDRVMALADVFEALTAADRPYKPAKTLSESLRIMAAMCRDQHLDTELFRYF 917 LRA + L R+ A+ DV++ALT +DRPYK A +E++ MA+ H D +F F Sbjct: 260 GLRAGAITLYSRMAAICDVYDALT-SDRPYKRAWDPAEAISAMASWT--GHFDRAMFLAF 316 Query: 918 LRS 920 +++ Sbjct: 317 VKT 319 >gi|300310565|ref|YP_003774657.1| response regulator containing a CheY-like receiver domain and an HD-GYP domain [Herbaspirillum seropedicae SmR1] Length = 348 Score = 69.7 bits (169), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 40/100 (40%), Positives = 59/100 (59%), Gaps = 2/100 (2%) Query: 794 NMGELHNLTVRRGTLTEEDRFAINNHIVQTLVMLKSLPWPDSLKSVPDTAANHHEKMDGS 853 MG ++ ++ G L EE+ + H +L + P P L++ A HHEK DGS Sbjct: 210 KMGIPDHILLKPGRLDEEELKVMRMHARIGADILVASPSP-LLQTASQIALTHHEKWDGS 268 Query: 854 GYPRRLRASTLPLQDRVMALADVFEALTAADRPYKPAKTL 893 GYP L+ +PL R++A+ADVF+ALT++ RPYKP +L Sbjct: 269 GYPNGLKGEQIPLYGRIVAVADVFDALTSS-RPYKPGWSL 307 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 68.2 bits (165), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 39/114 (34%), Positives = 67/114 (58%), Gaps = 7/114 (6%) Query: 808 LTEEDRFAINNHIVQTLVMLKSLPWPDSLKSVP-DTAANHHEKMDGSGYPRRLRASTLPL 866 LTEE+ ++ +H + +L+ LP + P D + +HHEKMDG+GYP +L + + Sbjct: 218 LTEEEFVSVQSHPLAGHRLLQELP---GIGDAPLDVSLHHHEKMDGTGYPYKLSGRDISM 274 Query: 867 QDRVMALADVFEALTAADRPYKPAKTLSESLRIMAAMCRDQHLDTELFRYFLRS 920 R+ A+ DV++A+T +DRPYK S S++ MA H D ++ + F+++ Sbjct: 275 TARMGAICDVYDAIT-SDRPYKSGWDPSRSIQHMAQ--SKGHFDFDVLQAFVKA 325 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,919,121 Number of extensions: 123414 Number of successful extensions: 449 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 444 Number of HSP's successfully gapped: 11 Length of query: 955 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 861 Effective length of database: 1,174,780 Effective search space: 1011485580 Effective search space used: 1011485580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)