BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0484.p (615 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309830|ref|YP_003773922.1| ATP-dependent DNA helicase [Her... 1212 0.0 gi|300311049|ref|YP_003775141.1| ATP-dependent RNA helicase [Her... 64 3e-11 gi|300312721|ref|YP_003776813.1| ATP-dependent RNA helicase [Her... 62 2e-10 >gi|300309830|ref|YP_003773922.1| ATP-dependent DNA helicase [Herbaspirillum seropedicae SmR1] Length = 618 Score = 1212 bits (3137), Expect = 0.0, Method: Compositional matrix adjust. Identities = 584/615 (94%), Positives = 602/615 (97%) Query: 1 MVDLDSLPPGATQQERALHVLETVFGYSSFRGHQGEIVQHVAAGGDALVLMPTGGGKSLC 60 MVD DSLP A QERALHVLETVFGYSSFRGHQGEIVQHVA GGDALVLMPTGGGKSLC Sbjct: 1 MVDFDSLPADAPPQERALHVLETVFGYSSFRGHQGEIVQHVAGGGDALVLMPTGGGKSLC 60 Query: 61 YQVPALLREGTGVVVSPLIALMQDQVDALAEVGVRAAFLNSTQSFDEALQIERRLRQGDL 120 YQVPALLR+GTGVV+SPLIALMQDQVDAL EVGVRAAFLNSTQSFDEA+QIERRLRQGDL Sbjct: 61 YQVPALLRQGTGVVISPLIALMQDQVDALDEVGVRAAFLNSTQSFDEAMQIERRLRQGDL 120 Query: 121 DVLYVAPERLMTPRCLDLLESARIALFAIDEAHCVSQWGHDFRPEYIKLSILHERFPQVP 180 D+LYVAPERLMTPRCLDLLESARI+LFAIDEAHCVSQWGHDFRPEYIKLS+LHERFPQVP Sbjct: 121 DLLYVAPERLMTPRCLDLLESARISLFAIDEAHCVSQWGHDFRPEYIKLSVLHERFPQVP 180 Query: 181 RIALTATADQQTREEIIHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDFIKSEHP 240 RIALTATADQQTREEIIHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDFIKSEHP Sbjct: 181 RIALTATADQQTREEIIHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDFIKSEHP 240 Query: 241 EDAGIVYCLSRKKVEETAEFLRGEGINALAYHAGMDYALRTKNQARFLREDKIVMVATIA 300 EDAGIVYCLSRKKVEETA+FLR EGINALAYHAGMDYA RT NQARFLREDKIVMVATIA Sbjct: 241 EDAGIVYCLSRKKVEETADFLRSEGINALAYHAGMDYAQRTANQARFLREDKIVMVATIA 300 Query: 301 FGMGIDKPDVRFVCHLDLPKSVEGYYQETGRAGRDGLPADAWMAYGLQDVVQQRRMIDES 360 FGMGIDKPDVRFVCHLDLPKSVEGYYQETGRAGRDGLPADAWMAYGLQDVVQQRRMIDES Sbjct: 301 FGMGIDKPDVRFVCHLDLPKSVEGYYQETGRAGRDGLPADAWMAYGLQDVVQQRRMIDES 360 Query: 361 EADETFKRVLGTKLDAMLSLCETLHCRRVHLLDYFGEKSSPCGNCDTCLTPPVSFDATVE 420 +ADETFKRVLGTKL+AML+LCETLHCRRVHLLDYFGEKS+PCGNCDTCLTPPVSFDATVE Sbjct: 361 DADETFKRVLGTKLNAMLALCETLHCRRVHLLDYFGEKSAPCGNCDTCLTPPVSFDATVE 420 Query: 421 VQKLLSTVYRVEQRFAPGHVIEVLRGIDGERVKQWRHDQLSVFGVGSDRGEAEWRAILRQ 480 VQKLLSTVYRVEQRFAPGHVIEVLRGIDGERVKQWRHDQLSVFGVGSDRGEAEWRAILRQ Sbjct: 421 VQKLLSTVYRVEQRFAPGHVIEVLRGIDGERVKQWRHDQLSVFGVGSDRGEAEWRAILRQ 480 Query: 481 VIALGLLTVDAENYSALKLTEAARPVLRGEQQVQLRRYRKPEKAKRSSQRSSFVETDLNS 540 VIALGLLTVDA+NYSALKLTEAARPVLRGEQ VQLRRY+KPEKAKRSSQRSSFVETDL++ Sbjct: 481 VIALGLLTVDADNYSALKLTEAARPVLRGEQAVQLRRYQKPEKAKRSSQRSSFVETDLST 540 Query: 541 EEQALFEKLRWWRMETARAHNVPAYVIFHDATMREIAKARPQSLDELRHVTGVGEKKLET 600 EEQALFEKLRWWR+ETARAHNVPAYVIFHDATMREIAKARPQSLD+LRHVTGVGEKKLET Sbjct: 541 EEQALFEKLRWWRVETARAHNVPAYVIFHDATMREIAKARPQSLDDLRHVTGVGEKKLET 600 Query: 601 YGAQIIELIAQMQAG 615 YGAQII LIA+MQA Sbjct: 601 YGAQIIALIAEMQAA 615 >gi|300311049|ref|YP_003775141.1| ATP-dependent RNA helicase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 64.3 bits (155), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 84/347 (24%), Positives = 137/347 (39%), Gaps = 51/347 (14%) Query: 26 GYSSFRGHQGEIVQHVAAGGDALVLMPTGGGKSLCYQVPALLREGTGVVVSPLIALMQDQ 85 GY+S Q + V V +GGD L TG GK+ + +P L R L A+ + + Sbjct: 20 GYTSPTPIQAQAVPAVLSGGDLLAGAQTGTGKTAGFTLPLLQR---------LSAMPRQK 70 Query: 86 VDALAEVGVRAAFLNSTQSFDEALQIERRLRQ--------------------------GD 119 ++ V +RA L T+ A Q+E +RQ Sbjct: 71 ING--HVPIRALILAPTREL--AAQVEESVRQYGKYLPLTSACIFGGVGINPQITLLKRG 126 Query: 120 LDVLYVAPERLMTPRCLDLLESARIALFAIDEAHCVSQWG--HDFRPEYIKLSILHERFP 177 +D+L P RL+ + I + +DEA + G D R +L P Sbjct: 127 VDILVATPGRLLDHMQQGTVNLQHIQILVLDEADRMLDMGFIRDIR------KVLAALPP 180 Query: 178 QVPRIALTAT-ADQQTR--EEIIHRLQLEDARQFVSSFDRPNIRYQIVEKANGRKQLLDF 234 + + +AT AD+ + + +++ L + + S+ + + V++ L Sbjct: 181 KRQNLLFSATFADEIKKLADSLLNNPALIEVARRNSTVEVIAQKIHPVDRDKKHPMLAHL 240 Query: 235 IKSEHPEDAGIVYCLSRKKVEETAEFLRGEGINALAYHAGMDYALRTKNQARFLREDKIV 294 IK+ H +V+ ++ + E L +GI A+A H + RTK A F Sbjct: 241 IKT-HQWKQVLVFTRTKHGANKLVEQLGRDGITAMAIHGNKSQSARTKALAEFKDGKLTA 299 Query: 295 MVATIAFGMGIDKPDVRFVCHLDLPKSVEGYYQETGRAGRDGLPADA 341 ++AT GID + V + DLP E Y GR GR G +A Sbjct: 300 LIATDIAARGIDIDQLPHVVNYDLPNVPEDYVHRIGRTGRAGATGEA 346 >gi|300312721|ref|YP_003776813.1| ATP-dependent RNA helicase [Herbaspirillum seropedicae SmR1] Length = 510 Score = 61.6 bits (148), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 87/355 (24%), Positives = 138/355 (38%), Gaps = 44/355 (12%) Query: 23 TVFGYSSFRGHQGEIVQHVAAGGDALVLMPTGGGKSLCYQVPALLREGTGVVVSPLIALM 82 T GY+S Q + + +G D LV TG GK+ + +P+L + + +P A Sbjct: 17 TEAGYTSPTPVQEKAIPAAISGQDLLVSSQTGSGKTAAFMLPSL-HQLADLPPAPQAART 75 Query: 83 QDQ------------------VDALAEVGVRAAFLNSTQSFDEALQIERRLR-------- 116 +Q L R L T + D+ RR+R Sbjct: 76 PNQERQASRARGERPRYQPAQPKMLVLTPTRELALQVTTATDKYGAYMRRVRVVSILGGM 135 Query: 117 --QGDLDVLYVAPERLM-TP-RCLDLLESARI-----ALFAIDEAHCVSQWGHDFRPEYI 167 + +L PE L+ TP R +D +ES +I + +DEA + G E I Sbjct: 136 PYPKQMQLLSRNPEILVATPGRLIDHMESGKIDFSQLQILVLDEADRMLDMGFIDDIEKI 195 Query: 168 KLSILHERFPQVPRIALTATADQQTREEIIHRLQLEDARQFVSSFDRPNI--RYQIVEKA 225 + R + L + + L ++ A SS NI R V+ Sbjct: 196 VAATPATRQTMLFSATLDGVVGNMAKRITNNPLTIQIAS---SSTRHENIMQRVHFVDDL 252 Query: 226 NGRKQLLDFIKSEHPEDAGIVYCLSRKKVEETAEFLRGEGINALAYHAGMDYALRTKNQA 285 + + +LLD + + D +++ +++ + A+ L G A A H M R + Sbjct: 253 SHKNRLLDHLLRDTSIDQAVIFTATKRDADTIADRLNIAGFAAAALHGDMHQGARNRTLN 312 Query: 286 RFLREDKIVMVATIAFGMGIDKPDVRFVCHLDLPKSVEGYYQ---ETGRAGRDGL 337 R ++VAT GID P + V + DLPK E Y TGRAGR+G+ Sbjct: 313 SLRRGQVRMLVATDVAARGIDVPGITHVFNYDLPKFAEDYVHRIGRTGRAGRNGV 367 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,943,555 Number of extensions: 84097 Number of successful extensions: 269 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 268 Number of HSP's successfully gapped: 3 Length of query: 615 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 524 Effective length of database: 1,188,985 Effective search space: 623028140 Effective search space used: 623028140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)