BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0475.p (402 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 745 0.0 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 355 3e-99 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 349 3e-97 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 157 2e-39 gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [... 146 3e-36 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 745 bits (1924), Expect = 0.0, Method: Compositional matrix adjust. Identities = 377/402 (93%), Positives = 387/402 (96%) Query: 1 MSSVSRFTRLTMTVAVAAALVACGQKPAAQGGPMPAPEVSVVTIAPQRVAITNELPGRVE 60 MSSVSRFTRLTMTVA+AAALVACGQKPAAQGGPMP EVSVVTIAPQRVAITNELPGRVE Sbjct: 1 MSSVSRFTRLTMTVAIAAALVACGQKPAAQGGPMPPSEVSVVTIAPQRVAITNELPGRVE 60 Query: 61 AFRTAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQ 120 AFR AQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQ Sbjct: 61 AFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQ 120 Query: 121 TTLTAQRYKPLVEVNAVSKMDYDNAVAAQKQAEADVASGKAAVQTASLNLGYATVTSPIS 180 TTLTAQRYKPLVEVNAVSK +YDNAVAAQKQAEADVA+GKAAVQTASLNLGYATVTSPIS Sbjct: 121 TTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPIS 180 Query: 181 GRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKNVGDN 240 GRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLK+VGDN Sbjct: 181 GRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDN 240 Query: 241 KAKVTLVLENGQPYDQPGKLLFSDVSVDESTGSVSLRAEFPNPKRLLLPGTYVRTNIEQA 300 KA VTL LENGQ Y PGKLLFSD+SVDE+TGSVSLRAEFPNPKRLLLPGTYVR IEQA Sbjct: 241 KASVTLELENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQA 300 Query: 301 VDDQALLVPQQAVMRDAGGSIVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIV 360 VD+QALLVPQQAV+RDAGGS VMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIV Sbjct: 301 VDEQALLVPQQAVVRDAGGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIV 360 Query: 361 EGLQKVRPGATVKPVQWSPAGAPAAATGQAAKPEASAQPKAN 402 EGLQKVRPGATVKPVQW+PAGAPA A+GQ AK EASAQPKAN Sbjct: 361 EGLQKVRPGATVKPVQWNPAGAPAQASGQPAKQEASAQPKAN 402 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 355 bits (912), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 184/362 (50%), Positives = 252/362 (69%), Gaps = 2/362 (0%) Query: 9 RLTMTVAVAAALVACGQKPAAQGGPMPAPEVSVVTIAPQRVAITNELPGRVEAFRTAQVR 68 RL +++ + A L AC + Q P EV V + QR A+T ELPGR A+ A +R Sbjct: 20 RLALSLILVAVLAACS-RSGDQPKPTAQAEVGYVVVKAQRQALTTELPGRTTAYLAADIR 78 Query: 69 ARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTLTAQRY 128 +V GI+QKR+FKEGS VK GQ L++IDPA Y+A+ +SA+A+L +A+A L + L A+RY Sbjct: 79 PQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEATLASSKLKAERY 138 Query: 129 KPLVEVNAVSKMDYDNAVAAQKQAEADVASGKAAVQTASLNLGYATVTSPISGRIGRALV 188 L +++AVSK D D+A + +Q +ADV KAAVQTA++NL Y + SPISGRI ++ V Sbjct: 139 AELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIEKSAV 198 Query: 189 TEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKNVGDNKAKVTLVL 248 T GALV T L VQQ+DP+YV+VTQS+ DLL+L++ +A+GQ++ G+N A+V +VL Sbjct: 199 TPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIRKSGENAAQVKIVL 258 Query: 249 ENGQPYDQPGKLLFSDVSVDESTGSVSLRAEFPNPKRLLLPGTYVRTNIEQAVDDQALLV 308 E+G Y++PGKL F+ +SVD TG ++LRA PNP +LLPG YVR +E+ VDD ALL Sbjct: 259 EDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVLEEGVDDHALLA 318 Query: 309 PQQAVMRD-AGGSIVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEGLQKVR 367 PQ+AV RD G + +V+ A+ KV+ R V A GNNW V GLKEGD++IV+GLQKVR Sbjct: 319 PQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVSRGLKEGDKLIVDGLQKVR 378 Query: 368 PG 369 G Sbjct: 379 TG 380 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 349 bits (895), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 188/367 (51%), Positives = 252/367 (68%), Gaps = 2/367 (0%) Query: 14 VAVAAALVACGQKPAAQGGPMPAPEVSVVTIAPQRVAITNELPGRVEAFRTAQVRARVAG 73 +A+ + L ACG+ P P + VVT+ Q V + ELPGR ++ A VR +V G Sbjct: 16 LAILSVLSACGKPPGGAPPAAGTPVLGVVTVHAQPVELHTELPGRTSPYQIADVRPQVNG 75 Query: 74 IVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVE 133 I++ R F+EGS+VK GQVL++I+PA Y+A+ +S+ A LA+A+A++ L A RYK LV Sbjct: 76 IIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKASRYKELVA 135 Query: 134 VNAVSKMDYDNAVAAQKQAEADVASGKAAVQTASLNLGYATVTSPISGRIGRALVTEGAL 193 + AVS+ DYD+AVA+ +AEADV S +A VQT+ +NL YA + +PISGRIG++ VT GAL Sbjct: 136 IKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKSSVTPGAL 195 Query: 194 VGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKNVGDNKAKVTLVLENGQP 253 V T L +QQLDP+YV+VTQSS L L++A+A GQL+ G N AKV LVLENG+ Sbjct: 196 VTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSG-NGAKVRLVLENGRT 254 Query: 254 YDQPGKLLFSDVSVDESTGSVSLRAEFPNPKRLLLPGTYVRTNIEQAVDDQALLVPQQAV 313 Y Q G L FSDV+V++ TG+++LRA FPNPK LLPG YVR +++ V Q LLVPQQAV Sbjct: 255 YAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQAV 314 Query: 314 MRD-AGGSIVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEGLQKVRPGATV 372 RD AG + VVNA+ VE R + T A G+ W V GLKEGD+++V+G Q+ GA V Sbjct: 315 SRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQLVVDGQQRASAGAKV 374 Query: 373 KPVQWSP 379 K V W+P Sbjct: 375 KVVPWTP 381 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 157 bits (396), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 108/323 (33%), Positives = 166/323 (51%), Gaps = 11/323 (3%) Query: 64 TAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTL 123 TA VRARV G + K +KEG VK G +L +IDP +A+L A+ LAR +A L L Sbjct: 59 TATVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARL 118 Query: 124 TAQRYKPLVEVNAVSKMDYDNAVAAQKQAEADVASGKAAVQTASLNLGYATVTSPISGRI 183 +RY+ L+ ++++ D VA KQ E V + + V +A L L + VT+PISGR+ Sbjct: 119 DLKRYQTLLAQDSIASQQVDTQVALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRL 178 Query: 184 GRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQL--KNVGDNK 241 G G V D L ++ QL P+ + ++ K+ Q + +G+ D + Sbjct: 179 GLRQADVGNNVTTSDTNGLVIITQLQPITAIFSIPEDNIPKVLQQLQSGRKLPAQAWDRE 238 Query: 242 AKVTLVLENGQPYDQPGKLLFSDVSVDESTGSVSLRAEFPNPKRLLLPGTYVRTNIEQAV 301 K L G LL D VD +TG+V L+A+FPN L P +V ++ Sbjct: 239 QKNKLA---------DGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNT 289 Query: 302 DDQALLVPQQAVMRDAGGSIVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVE 361 + A ++P A+ R + G V VV ++ V RPVKTG + + + DG+ G+ V+++ Sbjct: 290 EQGATVIPTAAIQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGETVVID 349 Query: 362 GLQKVRPGATVKPVQWSPAGAPA 384 G+ ++R GA V+ V A PA Sbjct: 350 GIDRLREGAKVEAVARGGADDPA 372 >gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 146 bits (368), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 114/338 (33%), Positives = 175/338 (51%), Gaps = 9/338 (2%) Query: 39 VSVVTIAPQRVAITNELPGRVEAFRTAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPA 98 V V + +++ GR+EA +VR V G + FK+G+ VK+G VLF IDP Sbjct: 51 VDVAEVVSRQIIDWQSYSGRIEAVDRVEVRPLVGGTLTAVHFKDGALVKKGDVLFTIDPR 110 Query: 99 QYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSKMDYDNAVAAQKQAEADVAS 158 Y+A + A+A LA A+A T R + L+ NA++K D++ A ++A A+VA+ Sbjct: 111 PYEAEVARAEAQLAGAEARAGYTASDLARGQRLLSDNAIAKRDFEEKQNASREASANVAA 170 Query: 159 GKAAVQTASLNLGYATVTSPISGRIGRALVTEGALVGQGDATQ-LALVQQLDPVYVNVTQ 217 +AA++ A LNL Y +T+P+SGRI RA VT G +V G A L + + VY + Sbjct: 171 ARAALRAARLNLEYTRITAPVSGRISRAEVTVGNVVNPGAANAPLTTLVSVAKVYASFDV 230 Query: 218 SSADLLK-LQQAMAAGQLKNVGDNKAKVTLVLENGQPYDQPGKLLFSDVSVDESTGSVSL 276 LK + A AAG V L L N Y + G++ D +D ++G++ + Sbjct: 231 DEQSFLKYVNPARAAGA-------TVPVELGLANEDGYSRKGRVGSVDNRLDTASGTIRV 283 Query: 277 RAEFPNPKRLLLPGTYVRTNIEQAVDDQALLVPQQAVMRDAGGSIVMVVNAENKVEPRPV 336 RA F N L+PG Y R + QA+L+ ++A+ D VMV + +N R V Sbjct: 284 RAIFDNANSQLVPGLYARVRLGGGEPHQAVLIDEKAIGTDQDKRFVMVADKDNHANYRQV 343 Query: 337 KTGGARGNNWQVLDGLKEGDRVIVEGLQKVRPGATVKP 374 G + GL+ G+R++V GLQ++RPG + P Sbjct: 344 VVGAGHDGLVVIEKGLQPGERIVVNGLQRIRPGDAITP 381 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.127 0.345 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,059,757 Number of extensions: 40646 Number of successful extensions: 190 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 11 Length of query: 402 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 315 Effective length of database: 1,207,925 Effective search space: 380496375 Effective search space used: 380496375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)