BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0473.p (483 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 909 0.0 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 436 e-123 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 412 e-116 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 385 e-108 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 244 9e-66 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 909 bits (2348), Expect = 0.0, Method: Compositional matrix adjust. Identities = 453/483 (93%), Positives = 475/483 (98%) Query: 1 MANLSLPRSPRLLQLCAALMVAGVLGGCSLAPTYERPQAPVESAYPADAAGRAELQAVNL 60 MANLS+PRSPRLL +CAAL++AGVLGGCSLAPTYERPQAPVES+YPADAAGRAELQAVNL Sbjct: 1 MANLSMPRSPRLLHMCAALVMAGVLGGCSLAPTYERPQAPVESSYPADAAGRAELQAVNL 60 Query: 61 GWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNIAGSAARTR 120 GWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLN++G+AARTR Sbjct: 61 GWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTR 120 Query: 121 TPASQSLTGQPVISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLV 180 T ASQSLTGQP ISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLV Sbjct: 121 TAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLV 180 Query: 181 AQVAQAYLSERSYAEQLQLANDTLKSREDNLELARKRFDVGASSALDFRLTQTLVESARV 240 AQVAQAYL+ERSYAEQL+LA++TLKSR+ NL+LA+KR DVGAS+ALDFRLTQTLVESARV Sbjct: 181 AQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARV 240 Query: 241 AKAQLERQRAQAENALTLLVGKKVGDLPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSA 300 AK QLERQRAQAENALTLLVGKK+G+LPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSA Sbjct: 241 AKTQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSA 300 Query: 301 EQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGNLFESGSGAWSFGPSLSLPIFDFG 360 EQSLLAANANIGAARAAFFPRISLTGNFGSASSTLG LFESGSGAWSFGPSLSLPIFDFG Sbjct: 301 EQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFG 360 Query: 361 RNSANLDLAEVRKNQAVVTYEKTIQTAFREVADALVARGTLDDQVKAQEAFLAAQQERLK 420 RNSANLDLAEVRKN+AVVTYEK IQTAFREVADALVARG+LD+QVKAQEAFLAAQQERLK Sbjct: 361 RNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLK 420 Query: 421 LTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGINENTVSVA 480 LTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGG+NENTVSVA Sbjct: 421 LTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTVSVA 480 Query: 481 AKQ 483 AKQ Sbjct: 481 AKQ 483 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 436 bits (1120), Expect = e-123, Method: Compositional matrix adjust. Identities = 251/487 (51%), Positives = 320/487 (65%), Gaps = 17/487 (3%) Query: 8 RSPRLLQLCAALMVAGVLGGCSLAPTYERPQAPVESAYPA----DAAGRAELQAV-NLGW 62 RSP +L + +M+A +LGGC+L P Y RP APV + + +AAG + V ++GW Sbjct: 8 RSP-VLPMMLPVMLAMLLGGCTLMPDYHRPDAPVAAHFAGADSKNAAGASSAPVVADIGW 66 Query: 63 RQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNIAGSAARTRTP 122 R F D LQQ+IA ALENNRDLR AVLNIE+ARAQY + RA L P++ A S +RTP Sbjct: 67 RDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTP 126 Query: 123 ASQSLTGQPVISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQ 182 S TG + S SY L AYELD FGRVR+LK+ AL S+LST EA++S QISL+A+ Sbjct: 127 GDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAE 186 Query: 183 VAQAYLSERSYAEQLQLANDTLKSREDNLELARKRFDVGASSALDFRLTQTLVESARVAK 242 VA A+L+ S ++L+LA DTL+S+ + L ++ F++G+SSAL R QT VESARV Sbjct: 187 VATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDV 246 Query: 243 AQLERQRAQAENALTLLVGKKVGDLPPAQL---LSDEKI-----VTDIPAGLPSDLLTNR 294 Q Q NAL LLVGK DLP A L L D ++ + IP GLPSDLL R Sbjct: 247 ESYTAQVDQDRNALVLLVGK---DLPTALLPQGLPDTRLAAASPLATIPPGLPSDLLQRR 303 Query: 295 PDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGNLFESGSGAWSFGPSLSL 354 DI AE+ L AANANIGAARAAF+P I+LT N G+AS+ L LF+ GSG+WSF P +SL Sbjct: 304 ADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISL 363 Query: 355 PIFDFGRNSANLDLAEVRKNQAVVTYEKTIQTAFREVADALVARGTLDDQVKAQEAFLAA 414 PIFD G N ANLD+A V ++ +V YEK IQTAFREV+DAL R TL Q++AQEA + A Sbjct: 364 PIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQA 423 Query: 415 QQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGINE 474 E +L+ R+ +G+ S L LD+QR L+SAQ+ LI + R N + YK LGGG E Sbjct: 424 SDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGWVE 483 Query: 475 NTVSVAA 481 T + AA Sbjct: 484 RTGTPAA 490 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 412 bits (1058), Expect = e-116, Method: Compositional matrix adjust. Identities = 228/462 (49%), Positives = 304/462 (65%), Gaps = 12/462 (2%) Query: 27 GCS-LAPTYERPQAPVESAYPADAAGR----------AELQAVNLGWRQFFPDQRLQQLI 75 GC+ LAP Y +P APV +++ + AA + E+ A +LGWR+FF D RLQQ+I Sbjct: 27 GCANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVI 86 Query: 76 AAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNIAGSAARTRTPASQSLTGQPVISN 135 AAAL+NNRDLR A LNIE+ARAQY+I A+ P+++ G+++ +RTPA S TG +S+ Sbjct: 87 AAALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSH 146 Query: 136 SYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLSERSYAE 195 V + V AYELDFFGR++NL DAAL SY E ++S ISLVA+VA A+L+ S + Sbjct: 147 QQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRD 206 Query: 196 QLQLANDTLKSREDNLELARKRFDVGASSALDFRLTQTLVESARVAKAQLERQRAQAENA 255 L+LA DT S+ L+++ D+G S +D + VESAR A+ Q Q NA Sbjct: 207 LLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNA 266 Query: 256 LTLLVGKKV-GDLPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAA 314 L LLVG + L PA L ++ PAGLPS +L RPD+ +AE +L +ANA+IGAA Sbjct: 267 LRLLVGADIDAALLPAGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAA 326 Query: 315 RAAFFPRISLTGNFGSASSTLGNLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKN 374 RAAFFP ISLT N G+AS TLG LF++GSG+W+F PS++LPIF+ G A+LD A++ ++ Sbjct: 327 RAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRD 386 Query: 375 QAVVTYEKTIQTAFREVADALVARGTLDDQVKAQEAFLAAQQERLKLTDLRFKNGIASSL 434 V YEK IQTAFREVADAL R TLD ++ AQ AA ++ +L+D R++NG+ S L Sbjct: 387 IDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSYL 446 Query: 435 DQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGINENT 476 L AQR L+SAQQ LI R +N I LYK LGGG +E T Sbjct: 447 TTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSEQT 488 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 385 bits (990), Expect = e-108, Method: Compositional matrix adjust. Identities = 222/451 (49%), Positives = 301/451 (66%), Gaps = 9/451 (1%) Query: 28 C-SLAPTYERPQAPVESAYPADAAGRAELQAVN------LGWRQFFPDQRLQQLIAAALE 80 C SLAP Y+RP APV + YP A Q N L WR +F D LQ LI ALE Sbjct: 25 CQSLAPHYQRPAAPVAAVYPDGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQALE 84 Query: 81 NNRDLRTAVLNIEEARAQYQIARADLLPNLNIAGSAARTRTPASQSLTGQPVISNSYQVG 140 N+RDLRTA L ++EA+A Y I ADL P + R+RTPA +LT +P++ ++YQ G Sbjct: 85 NSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQAG 144 Query: 141 LAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLSERSYAEQLQLA 200 L + ++E+D +GR+R+L DAAL S +++ + + +SL+AQVA A+L E+L++A Sbjct: 145 LGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEIA 204 Query: 201 NDTLKSREDNLELARKRFDVGASSALDFRLTQTLVESARVAKAQLERQRAQAENALTLLV 260 N T+ SR+++ + +R +VGA+S L+ +TL+ A+ AQL++QRAQ NALTLLV Sbjct: 205 NQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLV 264 Query: 261 GKKVGDLPPAQLLSD-EKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFF 319 G V LP Q + + + + GLP+ LLT RPD+ +AE L+AANANIGAARAAFF Sbjct: 265 GSPV-VLPVGQARAGLDAVFAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAFF 323 Query: 320 PRISLTGNFGSASSTLGNLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNQAVVT 379 P ISLTGN G+AS+ L LF GS AW+F PS++LPIF GR NL L+EVR++ AV Sbjct: 324 PSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAA 383 Query: 380 YEKTIQTAFREVADALVARGTLDDQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDA 439 YEKT+QTAFREVADAL AR L Q++ ++ + Q+ER +L+ LR+ NG A LD LDA Sbjct: 384 YEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDA 443 Query: 440 QRDLFSAQQALIQARQLRLNNAIDLYKALGG 470 QRDL SAQQ L+Q R+ L++++ LY ALGG Sbjct: 444 QRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 244 bits (624), Expect = 9e-66, Method: Compositional matrix adjust. Identities = 173/473 (36%), Positives = 260/473 (54%), Gaps = 9/473 (1%) Query: 13 LQLCAALMVAGVLGGCSLAPTYERPQAPVESAYPADAAGRAELQAVNLGWRQFFPDQRLQ 72 L LCA + L +L P+ E A PA+AA A +GW+ +FP LQ Sbjct: 15 LALCACAPLTAPLPPSALDLPTHYPRTLAEDA-PANAAPTAS----AIGWQDYFPQAPLQ 69 Query: 73 QLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNIAGSAARTRTPASQSLTGQPV 132 LIA AL +N DLR L +++ +AQ++I R+ LP +N+ A R+R P +G Sbjct: 70 ALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPESGPAA 129 Query: 133 ISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLSERS 192 + Y+VGL ++ELDF+GR+ +L +AAL ++++ A+++ ISL+ QVA A+ S R Sbjct: 130 STREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAWTSLRE 189 Query: 193 YAEQLQLANDTLKSREDNLELARKRFDVGASSALDFRLTQTLVESARVAKAQLERQRAQA 252 E+L L + ++L L + R D G + A ++ + R +A+L++ RA Sbjct: 190 LDERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQAELQQMRASQ 249 Query: 253 ENALTLLVGKKVGDLPPAQLLSDEKIVTD--IPAGLPSDLLTNRPDIRSAEQSLLAANAN 310 + L +L G + LP A + I+ + GLPS LL RPDI +AE L A+ A Sbjct: 250 RSTLEMLAG--LTQLPAALEPAPTTILLPFALAPGLPSTLLAARPDIIAAEHRLQASAAQ 307 Query: 311 IGAARAAFFPRISLTGNFGSASSTLGNLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAE 370 + AARAAFFPRISLT G AS+ L +LF+ S AW+F P +SLP+ D G + D+A Sbjct: 308 VAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYDIAA 367 Query: 371 VRKNQAVVTYEKTIQTAFREVADALVARGTLDDQVKAQEAFLAAQQERLKLTDLRFKNGI 430 + + +A++ YE+T+Q AFREVA L L +Q++ + + Q +R +R + G Sbjct: 368 LDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLRYGR 427 Query: 431 ASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGINENTVSVAAKQ 483 AS LD L+++RD AQQ L N I LY ALGGG + S ++Q Sbjct: 428 ASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGGTQALSASSPSQQ 480 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.350 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,242,931 Number of extensions: 47279 Number of successful extensions: 301 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 242 Number of HSP's successfully gapped: 19 Length of query: 483 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 394 Effective length of database: 1,198,455 Effective search space: 472191270 Effective search space used: 472191270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)