BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0462.p (364 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [H... 77 3e-15 gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 64 1e-11 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 58 1e-09 >gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [Herbaspirillum seropedicae SmR1] Length = 338 Score = 76.6 bits (187), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 68/291 (23%), Positives = 125/291 (42%), Gaps = 26/291 (8%) Query: 60 RFILHYGDMTDSSSLIRIIQQVQPDEIYNLAAQSHVAVSFEEPEYIANSDALGALRVLEA 119 R++ D+ D++++ R+ Q +P + + AA+SHV S P ++ G +L A Sbjct: 51 RYLFSQTDICDTAAIARLFAQHRPRAVVHCAAESHVDRSIAGPAVFIQTNVNGTFSMLSA 110 Query: 120 I----RILKLEKKT--RFYQASTSELYG-LVQEIPQ-KETTPFYPRSPYAVAKLYAYWIT 171 L ++ RF ST E++G L + P ET+P+ P SPY+ +K + + Sbjct: 111 AYDYWSTLPAAEQAAFRFLHISTDEVFGSLAADAPPFTETSPYAPNSPYSASKAASDHLV 170 Query: 172 VNYRESYGMYACNGILFNHESPVRGETFVTRKITRALSRIKLGLQECLYLGNLDAKRDWG 231 + +YG+ +L H S G K+ + L Q G+ RDW Sbjct: 171 RAFHSTYGL----PVLITHCSNNYGPFHFPEKLIPLVITNALAGQPLPIYGDGQQVRDWL 226 Query: 232 HAKDYVEMQWMMLQQETP-EDFVIATGVQYSVREFVDAAAREID-LAITWKGEGVNEKGF 289 H D+ ++L + P E + I + + E V +D LA +G+ ++ F Sbjct: 227 HVADHCRAIELVLARARPGESYNIGGNSEKTNLEVVLTLCDLLDELAPKAQGQYRDQICF 286 Query: 290 DSNGKCIVAVDPRYFRPAEVETLLGDATKAREKLGWTPKITFQELVSEMMR 340 + RP D +K + +LGW+P+ F++ + + ++ Sbjct: 287 VQD------------RPGHDRRYAVDTSKIQRELGWSPQHAFRQGLRQTVQ 325 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 64.3 bits (155), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 75/346 (21%), Positives = 149/346 (43%), Gaps = 44/346 (12%) Query: 9 LITGVTGQDGSYLAELLLEKGYEVHGIKRRASSFNTSRIDHLYQDPHERNPRFILHYGDM 68 L+TG G GS+ LL+ G+EV +F S + + + L GD+ Sbjct: 4 LVTGGAGYIGSHTCVELLKAGHEVIVF----DNFCNSSPESVKRVQQITGKTLSLVKGDI 59 Query: 69 TDSSSLIRIIQQVQPDEIYNLAAQSHVAVSFEEPEYIANSDALGALRVLEAIRILKLEKK 128 + L ++++ + + + + A V S E+P +++ +G +R+LEA++ + K Sbjct: 60 RNREQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGV--K 117 Query: 129 TRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGMYACNGI-L 187 T + +S++ +YG Q +P E P +PY +K+ + +Y ++ + + Sbjct: 118 TIVF-SSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRY 176 Query: 188 FN----HESPVRGE--TFVTRKITRALSRIKLGLQECLYLGNLD-------AKRDWGHAK 234 FN HES + GE + + ++++ +G +E L + D RD+ H Sbjct: 177 FNPVGAHESGLIGEDPGGIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVV 236 Query: 235 DYVEMQWMMLQQ-ETPEDFVI--ATGVQYSVREFVDAAAREIDLAITWKGEGVNEKGFDS 291 D LQ + P+ F + TGV YSV + + A + + + Sbjct: 237 DLARGHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPY------------ 284 Query: 292 NGKCIVAVDPRYFRPAEVETLLGDATKAREKLGWTPKITFQELVSE 337 A+ R RP ++ + + +A+ KLGW + + + ++ Sbjct: 285 ------AIAER--RPGDIASCYANPAEAKRKLGWEATLGLEAMCAD 322 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 58.2 bits (139), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 59/187 (31%), Positives = 84/187 (44%), Gaps = 17/187 (9%) Query: 9 LITGVTGQDGSYLAELLLEKGYEVHGIKRRASSFNTSRIDHLYQDPHE------RNPRFI 62 LITGV G GS L LL G +V G+ +F+T R +L + E N RFI Sbjct: 19 LITGVAGFIGSNLLARLLTLGQQVTGLD----NFSTGRQANLEEVRAEVGAAAWANFRFI 74 Query: 63 LHYGDMTDSSSLIRIIQQVQPDEIYNLAAQSHVAVSFEEPEYIANSDALGALRVLEAIRI 122 GD+ D+++ +R D + + AA V+ S E+P ++ G L +L A R Sbjct: 75 --EGDICDAATCLRACDGA--DYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARD 130 Query: 123 LKLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGMYA 182 + RF A++S YG +P+ E P SPYAV K + YGM + Sbjct: 131 AGV---ARFVYAASSSTYGDHPGLPKVEEVIGKPLSPYAVTKYVNELYAEVFARCYGMES 187 Query: 183 CNGILFN 189 FN Sbjct: 188 IGLRYFN 194 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,087,529 Number of extensions: 42684 Number of successful extensions: 143 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 3 Length of query: 364 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 278 Effective length of database: 1,212,660 Effective search space: 337119480 Effective search space used: 337119480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)