BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0446.p (471 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylam... 884 0.0 gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hy... 259 4e-70 gi|300312562|ref|YP_003776654.1| guanine deaminase [Herbaspirill... 57 4e-09 >gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylaminohydrolase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 884 bits (2284), Expect = 0.0, Method: Compositional matrix adjust. Identities = 432/471 (91%), Positives = 451/471 (95%) Query: 1 MNTFLIRNLQAILTGLPGEAARHAGPDIRVSDGVIAAIGKLDALPGENVIDAGDCVAYPA 60 M+TFLIRNLQAI+TGLPG AARHAGPDIRVSDGVIAAIG+LDALPGE +IDA CVAYPA Sbjct: 1 MSTFLIRNLQAIMTGLPGAAARHAGPDIRVSDGVIAAIGQLDALPGEELIDASHCVAYPA 60 Query: 61 WVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRAKFDAELFRLAARIGMVELMRSGC 120 WVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRA FDA+LFRLAARIGMVELMRSGC Sbjct: 61 WVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRASFDADLFRLAARIGMVELMRSGC 120 Query: 121 GTIADHNYLYYPGMPFDTSEILFEEAEKLGLRFVLCRGTATRTRQLEAELPSALRPETLD 180 GTIADHNYLYYPGMPFDTSEILFEEA++LGLRFVLCRGTATRTRQLEAEL SALRPETLD Sbjct: 121 GTIADHNYLYYPGMPFDTSEILFEEAQQLGLRFVLCRGTATRTRQLEAELSSALRPETLD 180 Query: 181 AFLTDMQRLARRFHDPAPASMRRVVMAPTTPLYSAAPEEMRAIADAARELGLRLHSHLSE 240 FL DMQ LA RFHDPAPASMRRVVMAPTTPLYSAAP+E+R +A AARE+GLRLHSHLSE Sbjct: 181 GFLADMQGLAGRFHDPAPASMRRVVMAPTTPLYSAAPDELREMAVAAREMGLRLHSHLSE 240 Query: 241 TVGYQDSAHAMHGCRPVEFCERIGWLGPDVWFAHLVKLDAEEIALLGSTGTGIAHCPQSN 300 TVGYQDSAHAMHGCRPVEFCER+GWLG DVWFAHLVKLDAEEI LLG TGTGIAHCPQSN Sbjct: 241 TVGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHCPQSN 300 Query: 301 GRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATPAFRG 360 GRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATP+FRG Sbjct: 301 GRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATPSFRG 360 Query: 361 GAFEGGADEATVEDVVRWGSTGGARVLGLDGLQGLQVGQQADIALYRLDDPRYFGLHDPA 420 G FEGGAD ATVEDVVRWG+TGGARVLGLD L GLQVGQQADIALYRLDDPRYFGLHDPA Sbjct: 361 GHFEGGADAATVEDVVRWGTTGGARVLGLDALDGLQVGQQADIALYRLDDPRYFGLHDPA 420 Query: 421 IGPVASGGRPFLRAMWVGGRAVVRDDAIPGVDLAELGAQARRAIKKMLEGN 471 IGPVASGGRPFL+AMWVGGRAVVR+DAIPGVDLAELGAQAR+A+KKML+ N Sbjct: 421 IGPVASGGRPFLKAMWVGGRAVVREDAIPGVDLAELGAQARQAVKKMLKSN 471 >gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hydrolase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 259 bits (662), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 170/466 (36%), Positives = 238/466 (51%), Gaps = 33/466 (7%) Query: 2 NTFLIRNLQAILTGLPGEAARHAGPD--IRVSDGVIAAIGKLDALP--GENVIDAGDCVA 57 T LIRN + ++T +R PD + + + VI +G+ LP + +IDA + Sbjct: 8 KTLLIRNARILVTM---NESREEIPDGALYIRNNVIEQVGQTADLPQDADEIIDARHHIV 64 Query: 58 YPAWVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRAKFDAELFRLAARIGMVELMR 117 P +NTHHH++QSL + P N L WL Y A E+ +++ M EL+ Sbjct: 65 LPGLINTHHHMYQSLTRAIPSAQNGELFNWLTKL-YPIWAGLTPEMIQVSTLTAMAELIL 123 Query: 118 SGCGTIADHNYLYYPGMPFDTSEILFEEAEKLGLRFVLCRGTATRTRQLEAELPSALRPE 177 SGC T +DH Y+Y D S E A ++G+RF RG A Q + LP + Sbjct: 124 SGCTTSSDHLYIYPNQTRLDDS---LEAAARIGMRFHGARG-AMSVGQSKGGLPPDRVVQ 179 Query: 178 TLDAFLTDMQRLARRFHDPAPASMRRVVMAPTTPLYSAAPEEMRAIADAARELGLRLHSH 237 A L D QRL +HD +M+R+V+AP +P +S + E MR AD AR G+ LH+H Sbjct: 180 DEAAILKDTQRLIETYHDHQRHAMQRIVVAPCSP-FSVSRELMREAADLARHYGVSLHTH 238 Query: 238 LSETVGYQDSAHAMHGCRPVEFCERIGWLGPDVWFAHLVKLDAEEIALLGSTGTGIAHCP 297 L+E V + P E+ E GW+G DVW AH V+LDA I L TGTG+AHCP Sbjct: 239 LAENVNDIAYSREKFNMSPAEYAEDCGWVGHDVWHAHCVQLDAHGIDLFARTGTGVAHCP 298 Query: 298 QSNGRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATPA 357 SN RL SGIAPIR + AGVPV +GVDG+ASN++A M+ E A L+QR Sbjct: 299 CSNMRLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLGEVRQAMLLQRV--------G 350 Query: 358 FRGGAFEGGADEATVEDVVRWGSTGGARVLGLDGLQGLQVGQQADIALYRLDDPRYFG-L 416 F G D T + + GGA+VL D + L+ G AD+ ++ + G L Sbjct: 351 F-------GPDAMTARQALEVATLGGAKVLNRDDIGALKPGMSADVVMFDTRQIAFAGAL 403 Query: 417 HDPAIGPVASGGRPFLRAMWVGGRAVVRDDAIPGVDLAELGAQARR 462 HDP + + + + GR +VR+ + + ELGA R Sbjct: 404 HDP-VAALVFCTPANVDCSIINGRVIVREGQLTSI---ELGAVLER 445 >gi|300312562|ref|YP_003776654.1| guanine deaminase [Herbaspirillum seropedicae SmR1] Length = 432 Score = 57.0 bits (136), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 95/387 (24%), Positives = 155/387 (40%), Gaps = 58/387 (14%) Query: 23 HAGPDIRVSDGVIAAIGKLD----ALPGENVI-DAGDCVAYPAWVNTHHHLFQSLLKGDP 77 H+ + V DG I A G +LP E V D + P +++TH H Q+ + G P Sbjct: 29 HSDGLLLVQDGKIRAAGDYAELSASLPPEAVTEDLRGKILCPGFIDTHVHYPQTDIIGSP 88 Query: 78 LGINATLTPWLAATPYRYRAKFDA-ELFRLAARIGMVELMRSGCGTIADHNYLYYPGMPF 136 L PWL + +F E A + EL+R+G + A +Y P Sbjct: 89 ---APGLLPWLDNYTFPAERRFQQPEHAAEVADFFLAELLRNGTTSAA----VYCTVHP- 140 Query: 137 DTSEILFEEAEKLGLRFVLCRGTATRTRQLEAELPSALRPETLDAFLTDMQRLARRFHDP 196 + + F + LR V + R P LR +T ++ + + + L R+H+ Sbjct: 141 QSVDAFFAASHARNLRMVAGKCLMDR------HAPDFLR-DTAESGVRESEALIGRWHNK 193 Query: 197 APASMRRVV-MAPTTPLYSAAPEEMRAIAD-AARELGLRLHSHLSETVGYQDSAHAMHGC 254 V APT+ E+M+ + AA+ + +H++E D + Sbjct: 194 GRQHYAITVRFAPTS-----THEQMKLAGELAAQYPDTYIQTHVAENT---DEVKWVKEL 245 Query: 255 RP-----VEFCERIGWLGPDVWFAHLVKLDAEEIALLGSTGTGIAHCPQSNGRLGSGIAP 309 P ++ + G + +AH + LD ++ A + +T + CP SN LGSG+ Sbjct: 246 YPDARSYLDVYDGYGLMRQRALYAHCIWLDDQDRARMAATQSAATLCPTSNLFLGSGLFD 305 Query: 310 IRALEAAGVPVSIGVD--GAASNEAADMISETHAAWLMQRARRGQEATPAFRGGAFEGGA 367 +AA +P+S+ D G S ++E L + AR G PA R Sbjct: 306 FARADAARLPLSVATDVGGGTSFSMLQTMNE-----LYKVARMGGTYLPAQR-------- 352 Query: 368 DEATVEDVVRWGSTGGARVLGLDGLQG 394 + + GGAR L L+G G Sbjct: 353 -------MFYLATLGGARALQLEGTIG 372 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,659,909 Number of extensions: 75501 Number of successful extensions: 253 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 249 Number of HSP's successfully gapped: 3 Length of query: 471 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 382 Effective length of database: 1,198,455 Effective search space: 457809810 Effective search space used: 457809810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)