BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0440.p (339 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 522 e-149 gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 217 1e-57 gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredox... 202 5e-53 gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation N... 69 6e-13 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 55 8e-09 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 522 bits (1345), Expect = e-149, Method: Compositional matrix adjust. Identities = 288/339 (84%), Positives = 302/339 (89%) Query: 1 MNAVTDRFNGNAATTLRLRVRAVRHAAEGIRLFELVAQEDAALPPWTPGAHLRLHLQDGL 60 MNAV DRFNG+AATTL LRVRAVR AA+ IRL ELV+Q+ AALPPWTPGAHLRL LQDGL Sbjct: 1 MNAVADRFNGSAATTLMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGL 60 Query: 61 VRAYSLCNTPQQRDFYVIAVKRESDSRGGSAFLHEQVQQGDSISASPPVNAFPLIADPRA 120 VRAYSLCNTPQQRD Y+IAVKRE DSRGGSAFLHEQVQ+G +ISASPPVNAFPLIAD RA Sbjct: 61 VRAYSLCNTPQQRDCYLIAVKREPDSRGGSAFLHEQVQEGTTISASPPVNAFPLIADVRA 120 Query: 121 PLLLAAGIGITPLHAMAAEMAAQRRRHRLHYFARSLAHAAFFHELAACSQGMSLHLGLDG 180 PLLLAAGIGITPLHAMAAE+A Q RRHRLHYFARSLAHAAFFHELAA SQGMSLHLGLDG Sbjct: 121 PLLLAAGIGITPLHAMAAELATQGRRHRLHYFARSLAHAAFFHELAAGSQGMSLHLGLDG 180 Query: 181 SETERAIAAALAAVPSSSPLYVCGPAPFIGAARRHAQLAGWHESDIHFEWFAAPVMANAE 240 +ETE AIAAALAA P SSPLYVCGP+PFI AARRHAQLAGW E DIHFE FAAP M AE Sbjct: 181 AETEHAIAAALAAAPRSSPLYVCGPSPFIDAARRHAQLAGWREQDIHFERFAAPAMPAAE 240 Query: 241 PQSAAQAHAEERVFELVLQRSGLRCEVQPGQSIVAAAAQLGVVIGTSCGEGFCGSCESTV 300 A A + FELVLQRSGLRC+V PGQSIVAAAAQ+GVVIGTSCGEGFCGSCESTV Sbjct: 241 ADPARPVQAPDSTFELVLQRSGLRCQVLPGQSIVAAAAQVGVVIGTSCGEGFCGSCESTV 300 Query: 301 LDGQPLHRDSVLSDAERASGRRILPCVSRCAGTRLVLDL 339 L+GQP HRDSVLS AERASGRRILPCVSRCAGTRLVLDL Sbjct: 301 LEGQPWHRDSVLSAAERASGRRILPCVSRCAGTRLVLDL 339 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 217 bits (552), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 136/329 (41%), Positives = 186/329 (56%), Gaps = 15/329 (4%) Query: 14 TTLRLRVRAVRHAAEGIRLFELVAQEDAALPPWTPGAHLRLHLQDGLVRAYSLCNTPQQR 73 TTL+++V A R A I ELVA + AALP +T GAH+ +HL GLVR YSLCN P ++ Sbjct: 2 TTLQVKVAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHEQ 61 Query: 74 DFYVIAVKRESDSRGGSAFLHEQVQQGDSISASPPVNAFPLIADPRAPLLLAAGIGITPL 133 Y I V R+ +SRGGS +HE ++ GD ++ S P N F L+ + LLLA GIG+TP+ Sbjct: 62 HRYQIGVLRDVNSRGGSVAVHEALKLGDILTISAPRNQFALVPAQHS-LLLAGGIGVTPI 120 Query: 134 HAMAAEMAAQRRRHRLHYFARSLAHAAFFHELAACS-QGMSLHLGLDGSETER-AIAAAL 191 MA +AA +HY ARS A AF +AA +H DG ++ + A L Sbjct: 121 LCMAEALAAAGASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALL 180 Query: 192 AAVPSSSPLYVCGPAPFIGAARRHAQLAGWHESDIHFEWF-AAPVMANAEPQSAAQAHAE 250 A ++ LYVCGPA FI A+ GW E+ +HFE+F AAPV A+ Sbjct: 181 AQADPATHLYVCGPAGFIAHVVDTARARGWPEAQVHFEYFGAAPVKADG----------- 229 Query: 251 ERVFELVLQRSGLRCEVQPGQSIVAAAAQLGVVIGTSCGEGFCGSCESTVLDGQPLHRDS 310 FE+ L SG + P ++++ + GV + SC +G CG+C + VL+G+P HRD Sbjct: 230 NGAFEVKLASSGQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQ 289 Query: 311 VLSDAERASGRRILPCVSRCAGTRLVLDL 339 L+D ERA+ + PC SR LVLDL Sbjct: 290 YLTDEERAANDQFTPCCSRARSALLVLDL 318 >gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 202 bits (513), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 131/326 (40%), Positives = 172/326 (52%), Gaps = 10/326 (3%) Query: 16 LRLRVRAVRHAAEGIRLFELVAQEDAALPPWTPGAHLRLHLQDGLVRAYSLCNTPQQRDF 75 ++L V + IR F LV + A LP + PGAH+ + G++R YSLC + R Sbjct: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 Query: 76 YVIAVKRESDSRGGSAFLHEQVQQGDSISASPPVNAFPLIADPRAPLLLAAGIGITPLHA 135 Y IAVKRES SRGGSA LH+ V+ G + P N F L A LL AGIG+TPL A Sbjct: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 Query: 136 MAAEMAAQRRRHRLHYFARSLAHAAF--FHELAACSQGMSLHLGLDGSETERAIAAALAA 193 MA + A+ +R LHYF R+ HAAF E A + + H + ++ E + L A Sbjct: 121 MAYRLWAEEKRFTLHYFVRAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTVCLEA 180 Query: 194 VPSSSPLYVCGPAPFIGAARRHAQLAGWHESDIHFEWFAAPVMANAEPQSAAQAHAEERV 253 + +Y CGPAPF+ A + A A E IH E F A A A Sbjct: 181 GSKEAHVYTCGPAPFMDAVVKCAS-ATRSEEAIHLEHFQAAPAAPAAAGGDGA------- 232 Query: 254 FELVLQRSGLRCEVQPGQSIVAAAAQLGVVIGTSCGEGFCGSCESTVLDGQPLHRDSVLS 313 FE+ L SG ++ G IV A+ G+VI TSC EG CG+C +L+G+P HRD+ LS Sbjct: 233 FEVKLASSGKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLS 292 Query: 314 DAERASGRRILPCVSRCAGTRLVLDL 339 E+A+ +I CVSR LVLDL Sbjct: 293 KKEKAANDQICTCVSRARSGMLVLDL 318 >gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 68.9 bits (167), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 81/322 (25%), Positives = 130/322 (40%), Gaps = 33/322 (10%) Query: 46 WTPGAHLRLHLQ---DGLVRAYSLCNTPQQRDFYVIAVKRESDSRGG--SAFLHEQVQQG 100 + G HL L + + L R+YS+C+ Q+R V A+KR + GG S + +E G Sbjct: 36 YQQGQHLTLRSEINGEDLRRSYSICSAVQERQLRV-AIKR---APGGLFSNWANESFVPG 91 Query: 101 DSISASPPVNAF--PLIADPRAPLL-LAAGIGITPLHAM-AAEMAAQRRRHRLHYFARSL 156 I PP+ F PL A R L AAG GITP+ ++ + ++ H +A Sbjct: 92 QRIDVMPPMGHFNVPLEAGNRKHYLAFAAGSGITPMMSIIKTTLLSEPHSHFTLVYANRA 151 Query: 157 AHAAFFHE------------------LAACSQGMSLHLGLDGSETERAIAAALAAVPSSS 198 + + F E ++ Q + L G A A+ + Sbjct: 152 SSSVIFKEELTDLKDAYLERFNVVYVMSREQQDVELFNGRIDRAKCDAFFASWIDLKDVD 211 Query: 199 PLYVCGPAPFIGAARRHAQLAGWHESDIHFEWFAAPVMANAEPQSAAQAHAEERVFELVL 258 ++CGP + A Q G ++ I E FAA A E V +V Sbjct: 212 AAFLCGPEEMVQAVAASLQAHGLPKTQIKTELFAASTPPRAHAARTVVGKQECEVTVIVD 271 Query: 259 QRSGLRCEVQPGQSIVAAAAQLGVVIGTSCGEGFCGSCESTVLDGQ-PLHRDSVLSDAER 317 + + +S++ A + G+ + SC G C +C V++G+ + + L D E Sbjct: 272 GYHNVFTMDKEKESVLEAGLKHGIDLRYSCKGGVCATCRCKVVEGKVDMDANYALEDYEI 331 Query: 318 ASGRRILPCVSRCAGTRLVLDL 339 A G +L C S +L+LD Sbjct: 332 ARG-FVLSCQSFPVSDKLLLDF 352 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 55.1 bits (131), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 65/228 (28%), Positives = 95/228 (41%), Gaps = 27/228 (11%) Query: 27 AEGIRLFELVAQEDAALPPWTPGAHLRLHLQ-DG--LVRAYSL------CNTPQQRDFYV 77 +E I F V Q+ L + PG ++ L + DG R YSL P Q Y Sbjct: 167 SEEITSFHFVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQAGQPPQ---YR 223 Query: 78 IAVKRESDSRGGSAFLHEQVQQGDSISASPPVNAFPLIADPRAPLLLAAGIGITPLHAM- 136 I+VKRE + S LH+Q+ G + PP F L+A + L++ G+GITP+ +M Sbjct: 224 ISVKREPGGKV-SRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALISGGVGITPMMSML 282 Query: 137 AAEMAAQRRRHRLHYFARSLAHAAF--FHELAACSQGMSLHLGLDGSETERAIAAALAAV 194 AA + + R H +H HA ELAA + L + A A + Sbjct: 283 AAALPSGRPIHFIHAARHGGVHAFRRQLEELAARHPQLQLRFCYEQPRQGDAAPHATGLI 342 Query: 195 ---------PSSSPL--YVCGPAPFIGAARRHAQLAGWHESDIHFEWF 231 P + L Y GP F+ A +R G + +E+F Sbjct: 343 ERSLLQQWLPGNPDLDAYFVGPKSFMKAIKRQLAELGVPAAQSRYEFF 390 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.133 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 942,798 Number of extensions: 36004 Number of successful extensions: 139 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 6 Length of query: 339 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 253 Effective length of database: 1,212,660 Effective search space: 306802980 Effective search space used: 306802980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)