BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0434.p (145 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309792|ref|YP_003773884.1| hypothetical protein Hsero_0451... 187 4e-49 >gi|300309792|ref|YP_003773884.1| hypothetical protein Hsero_0451 [Herbaspirillum seropedicae SmR1] Length = 146 Score = 187 bits (474), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 121/146 (82%), Positives = 130/146 (89%), Gaps = 1/146 (0%) Query: 1 MKPSNVWFQQSLAAALLCVSAGLAAPLAVAQPAPPGLAAQPAPPPPELEKLEEGEPPAVT 60 MKPS WF++SLAA LLC SAGLAAPLA+AQPAP G+AAQPAPPPPELEKLEEGEPPAVT Sbjct: 1 MKPSQPWFKESLAAVLLCASAGLAAPLALAQPAPAGVAAQPAPPPPELEKLEEGEPPAVT 60 Query: 61 IRKPGEDGRNQGNSIKERRDHGQTKEIEVKSGPSTYYIRPNPQLGGSTPGDAQSPPPSSA 120 IRKPGED RNQGNSIKE+RDHGQ KEIEVKSGPSTYY+RPN +G STPGDAQS PP+SA Sbjct: 61 IRKPGEDSRNQGNSIKEQRDHGQVKEIEVKSGPSTYYLRPNQNVGSSTPGDAQSGPPTSA 120 Query: 121 QWKVGEFDWGVKKP-QEGDTDNTPKK 145 QWKVGEFDWGVKKP QEG T+ KK Sbjct: 121 QWKVGEFDWGVKKPQQEGQTEGAAKK 146 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.307 0.130 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 409,904 Number of extensions: 14246 Number of successful extensions: 18 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 18 Number of HSP's successfully gapped: 1 Length of query: 145 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 68 Effective length of database: 1,255,275 Effective search space: 85358700 Effective search space used: 85358700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (22.0 bits) S2: 126 (53.1 bits)