BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0430.p (490 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 885 0.0 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 502 e-143 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 138 1e-33 gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 117 3e-27 gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 95 1e-20 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 885 bits (2288), Expect = 0.0, Method: Compositional matrix adjust. Identities = 454/488 (93%), Positives = 466/488 (95%) Query: 1 MYLGIDLGTSEVKAVVIDDQGDLLALAGSTLNVTRAHPRWSEQAPADWWQATCDTVDKLR 60 MYLGIDLGTSEVKAVVID QG L+ALAGSTLNV R HPRWSEQAPADWWQATCDTV KLR Sbjct: 1 MYLGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLR 60 Query: 61 TQLGRERFAGIRAIGLSGQMHGAVLLDEDDEVLRPAILWSDSRSAPECAELDRRAPRLHG 120 TQLG ERF IRAIGLSGQMHGAVLLDE DEVLRPAILWSDSRSAPECAEL+ RAPRLHG Sbjct: 61 TQLGSERFGSIRAIGLSGQMHGAVLLDERDEVLRPAILWSDSRSAPECAELESRAPRLHG 120 Query: 121 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWL 180 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWL Sbjct: 121 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWL 180 Query: 181 DVEGRNWSDELLAASGMRREQMPALVDGSAVSGTLLPDVARAWGLGENVIVAGGAGDGAA 240 DVEGRNWSDELLAASGMRR+QMPALVDGSAVSG+LLP+VA+AWGL +VIVAGGAGDGAA Sbjct: 181 DVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVIVAGGAGDGAA 240 Query: 241 SAVGIGAVNPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAAS 300 SAVGIGAV PGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAAS Sbjct: 241 SAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAAS 300 Query: 301 CLRWFCRLCSVDEKSLLAEIAELDHEARSNAPLFLPYLSGERTPHNDPYATGVFHGLTPE 360 CLRWFCRLCSVDEKSLLAEI +LD +A +NAPLFLPYLSGERTPHNDPYATGVFHGLTPE Sbjct: 301 CLRWFCRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGERTPHNDPYATGVFHGLTPE 360 Query: 361 HQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALAVRIV 420 HQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADAL VRIV Sbjct: 361 HQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIV 420 Query: 421 THVGSEAGGALGAARLAWLAAGGDEAQVCRKPARQAQYQPDPTRHAALQVRLQRYRDIYA 480 THVGSEAGGALGAARLAWLA GGDEA+VCRKPA+QA YQPDP RHAALQ RLQRYR IYA Sbjct: 421 THVGSEAGGALGAARLAWLADGGDEAEVCRKPAQQALYQPDPARHAALQARLQRYRAIYA 480 Query: 481 PFAPLHGE 488 PFAPLHGE Sbjct: 481 PFAPLHGE 488 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 502 bits (1293), Expect = e-143, Method: Compositional matrix adjust. Identities = 262/483 (54%), Positives = 323/483 (66%), Gaps = 2/483 (0%) Query: 2 YLGIDLGTSEVKAVVIDDQGDLLALAGSTLNVTRAHPRWSEQAPADWWQATCDTVDKLRT 61 YLGIDLGTSEVK V+ D+ +++A + L V HP WSEQAP WW AT D + LRT Sbjct: 3 YLGIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAALRT 62 Query: 62 QLGRERFAGIRAIGLSGQMHGAVLLDEDDEVLRPAILWSDSRSAPECAELDRRAPRLHGI 121 Q + F +R IGLSGQMHGA LLD + VLRPAILW+D R+ EC EL+ P I Sbjct: 63 Q-APQAFQALRGIGLSGQMHGATLLDRNGNVLRPAILWNDMRAHAECVELEALVPDAVAI 121 Query: 122 AGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWLD 181 GN AMPGFTAPKLLW++++EP ++ I VLLPKD+L K+TG VS+ SDAAGTLWLD Sbjct: 122 TGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDFLGWKLTGEFVSEMSDAAGTLWLD 181 Query: 182 VEGRNWSDELLAASGMRREQMPALVDGSAVSGTLLPDVARAWGLGENVIVAGGAGDGAAS 241 V R+WS+ +L A+G+ R MP LV+GSAV+G L ++ R WG+ V+VAGGAGD AAS Sbjct: 182 VARRDWSERMLGATGLDRSHMPRLVEGSAVAGQLRDELRREWGISGPVVVAGGAGDNAAS 241 Query: 242 AVGIGAVNPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAASC 301 AVGIG + GD FLSLG+SGVLF PN + +HAFCH LP +WHQMSV+LSAA+ Sbjct: 242 AVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNAQQGVHAFCHCLPGQWHQMSVILSAAAS 301 Query: 302 LRWFCRLCSVDEKSLLAEIAELDHEARSNAPLFLPYLSGERTPHNDPYATGVFHGLTPEH 361 L W + + L++ L ++ APLFLPYL+GERTPHND A GV G+TP H Sbjct: 302 LHWLSGVTARPVAELVSGAERLSAGQQAQAPLFLPYLNGERTPHNDAAAKGVLFGMTPAH 361 Query: 362 QRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALAVRIVT 421 + A L YAV+EGVAF M DG AL+AAGT + + VGGG+RSA+W L A AL + + Sbjct: 362 ESAHLAYAVMEGVAFAMADGYAALQAAGTTLESAAFVGGGSRSAFWGVLCATALGIPLRR 421 Query: 422 HVGSEAGGALGAARLAWLA-AGGDEAQVCRKPARQAQYQPDPTRHAALQVRLQRYRDIYA 480 H G+E G ALGAARL LA G D A VC P Y+PD R A LQ RL +YR +Y Sbjct: 422 HAGAEVGAALGAARLGRLARTGEDAASVCIPPEVLEWYRPDAARQAQLQRRLAQYRRLYR 481 Query: 481 PFA 483 A Sbjct: 482 SLA 484 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 138 bits (348), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 130/454 (28%), Positives = 199/454 (43%), Gaps = 26/454 (5%) Query: 3 LGIDLGTSEVKAVVIDDQGDLLALAGSTLNVTRAHPRWSEQAPADWWQATCDTVDKLRTQ 62 LG+D+GT+ K+VV G ++A V P +EQ P A ++ Sbjct: 11 LGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAVKA 70 Query: 63 LGRERFAGIRAIGLSGQMHGAVLLDEDDEVLRPAILWSDSRSA--PECAELDRRAPRLHG 120 I + S MH + LD ++ +L +I W D R++ E + + ++ Sbjct: 71 AKAAP-QEIALLSFSAAMHSVIALDAENRLLSNSITWGDIRASVWAERIKHEHDGNAIYR 129 Query: 121 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWL 180 G P KL+W+ +P +F R A + K++L ++ G+ V D S A+ T Sbjct: 130 RTGTPVHPMSPLCKLMWMRHEKPDVFHRAARFVGIKEYLLYQLFGQWVVDHSIASATGMF 189 Query: 181 DVEGRNWSDELLAASGMRREQMPALVDGSAVSGTLLPDVARAWGLGENVIVAGGAGDGAA 240 ++ W LA G+R +Q+P V + L ++A+ GL + GA DG Sbjct: 190 NLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSEMAQRLGLSVDTPFIIGANDGVL 249 Query: 241 SAVGIGAVNPGDGFLSLGTSGVLFVVNDRFRPNP-GRAIHAFCHTLPQR-WHQMSVMLSA 298 S +G+ A+ G +++GTSG + V D R +P GR FC+ L ++ W + + Sbjct: 250 SNLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGR---LFCYALTEKHWVVGGPVNNG 306 Query: 299 ASCLRWF-----------CRLCSVDEKSLLAEIAELDHEARSNAP--LFLPYLSGERTPH 345 + RW R VD L IAE + R A LF P+++GER P Sbjct: 307 GNIFRWVRDELATAEAAAAREEGVDPYEALTRIAE---KVRPGAEGLLFHPFMAGERAPL 363 Query: 346 NDPYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSA 405 + G F GL H + + A LEGV F + L AL + GG ARSA Sbjct: 364 WNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPALEELVGPTKRMMATGGFARSA 423 Query: 406 YWAQLLADALAVRIVTHVGSEAGGALGAARL-AW 438 W Q++AD +V E+ LGAA L AW Sbjct: 424 LWRQMMADIFNREVVVPESVES-SCLGAAVLGAW 456 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 117 bits (292), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 131/498 (26%), Positives = 216/498 (43%), Gaps = 42/498 (8%) Query: 3 LGIDLGTSEVKAVVIDDQGDLLALAGSTLNVTRAHPRWSEQAPADWWQATCDTVDKLRTQ 62 L +D GTS ++++ D G ++A A P W E P D W + T K+ Q Sbjct: 5 LALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQKVLAQ 64 Query: 63 LGRERFAGIRAIGLSGQMHGAVLLDE-DDEVLRPAILWSDSRSAPECAELDRR--APRLH 119 G + + + A+G++ Q V+ + + + A++W D R+ CA L R + Sbjct: 65 SGI-KASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGEAIQ 123 Query: 120 GIAGNLAMPGFTAPKLLWVARHEPQLFAR-------IATVLLPKDWLRLKMTGRK--VSD 170 G + P F+ KL W+ H L AR TV WL +TG + VSD Sbjct: 124 KKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTV---DSWLVWNLTGGRLHVSD 180 Query: 171 PSDAAGTLWLDVEGRNWSDELLAASGMRREQMPALVDGSAVSGTLLPDVARAWGLGENVI 230 S+A+ T+ ++ W +ELL G+ +P + + + G + A LG V Sbjct: 181 VSNASRTMLWNIHTGAWDEELLQWLGIPAAMLPQVQPSAHLYGEI-----DAQHLGAAVK 235 Query: 231 VAGGAGDGAASAVGIGAVNPGDGFLSLGTSGVLFV-VNDRF-RPNPGRAIHAFCHTLPQR 288 + G AGD ++ G V PG + GT + + D+ + G A C Q+ Sbjct: 236 IGGIAGDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDEQK 295 Query: 289 WHQM--SVMLSAASCLRWF-----CRLCSVDEKSLLAEIAELDHEARSNAPLFLPYLSGE 341 + + SV + A ++W S D + L + + S +F+P +G Sbjct: 296 QYALEGSVFIGGA-VVQWLRDGLKAIKTSTDVEGLACSVED------SGGVVFVPSFTGL 348 Query: 342 RTPHNDPYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDAL-RAAGTDVAELSLVGG 400 P+ +P A G GL+ A + A LE +AF L+A+ R A + + EL + GG Sbjct: 349 GAPYWNPSAKGAIVGLSRGSTVAHIARAALESIAFQSTALLNAMVRDAVSPITELRVDGG 408 Query: 401 GARSAYWAQLLADALAVRIVTHVGSEAGGALGAARLAWLAAG--GDEAQVCRKPARQAQY 458 A + Q AD L + ++ +E ALGAA LA +A G D +++ + + + Sbjct: 409 AAANNLLLQFQADLLGIPVIRPQVTET-TALGAAYLAGIATGVYRDLSELSSQWRMEHTF 467 Query: 459 QPDPTRHAALQVRLQRYR 476 +P R A Q R+ + Sbjct: 468 EPSIGRDQA-QARIHAWE 484 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 95.1 bits (235), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 125/482 (25%), Positives = 184/482 (38%), Gaps = 44/482 (9%) Query: 3 LGIDLGTSEVKAVVIDDQGDLLALAGSTLNVTRAHPRWS-EQAPADWWQATCDTVDKLRT 61 +GID+GT +A V D QG LLA A +++ R R EQ+ A W+A C V T Sbjct: 6 IGIDVGTGSARAGVFDPQGQLLASARHDIDLFRDERRARVEQSSAQIWEAVCQAVRAAVT 65 Query: 62 QLGRERFAGIRAIGLSGQMHGAVL-----LDEDDEVLRPAILWSDSRSAPECAELDRRAP 116 + G + A + IG+ V + + R I+W D R+ + ++R Sbjct: 66 KSGIDP-AAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRINRMGH 124 Query: 117 RLHGIAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAG 176 + G + P PKLLW+ + P+++A A D+L K TG Sbjct: 125 AVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSLQRSSCTVTC 184 Query: 177 TLWLDVEGRNWSDEL--------LAASGMRREQMPALVDGSAVSGTLLPDVARAWGLGEN 228 W LA +G R + G+ ++G L A+A GL Sbjct: 185 KWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMGLRPG 244 Query: 229 VIVAGGAGDGAASAVGIGAVNPGDG------FLSLGTSGVLFVVNDRFRPNPGRAIHAFC 282 + VA G D A VG A G G GTS + PG + Sbjct: 245 IAVAAGLIDAHAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPVLVPGVWGPYYK 304 Query: 283 HTLPQRWHQMSVMLSAASCLRWFCRL-------------CSVDEKSLLAE--IAELDHEA 327 P W +A + + RL ++ LA+ +A +DH + Sbjct: 305 AMAPGMWLNEGGQSAAGAAIDHLLRLHPATPQAREEAARAQMELPQWLAQQALAAVDHPS 364 Query: 328 R----SNAPLFLPYLSGERTPHNDPYATGVFHGLTPEH---QRAALGYAVLEGVAFGMVD 380 + +P G R+P DP A GL EH AL A L + +G+ Sbjct: 365 EAVWLAGQLTVVPEFLGNRSPLADPQARAALVGLGMEHDIDSLVALYVAGLCSLGYGLRQ 424 Query: 381 GLDALRAAGTDVAELSLVGGGARSAYWAQLLADALAVRIVTHVGSEAGGALGAARLAWLA 440 ++A A G V +S+ GG QLLADA + + E LG+A LA +A Sbjct: 425 IIEAQAACGVRVQSISVSGGAGVHPLTRQLLADATGMPVEITASPEP-VLLGSAMLAAVA 483 Query: 441 AG 442 AG Sbjct: 484 AG 485 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,629,288 Number of extensions: 70396 Number of successful extensions: 255 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 244 Number of HSP's successfully gapped: 5 Length of query: 490 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 401 Effective length of database: 1,198,455 Effective search space: 480580455 Effective search space used: 480580455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)