BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.0422.p
         (301 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309779|ref|YP_003773871.1| fructokinase [Herbaspirillum se...   524   e-150
gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp...    55   7e-09

>gi|300309779|ref|YP_003773871.1| fructokinase [Herbaspirillum
           seropedicae SmR1]
          Length = 301

 Score =  524 bits (1349), Expect = e-150,   Method: Compositional matrix adjust.
 Identities = 278/301 (92%), Positives = 291/301 (96%)

Query: 1   MTQSAAFVVFGEALTDFIRQPDGQWRAIPGGACWNVARAAARLGVPTGYAGSVSTDVFGA 60
           MTQSAAFVVFGEALTDFIRQPD QWRAIPGGACWNVARAAARLGVPTGYAGSVSTDVFGA
Sbjct: 1   MTQSAAFVVFGEALTDFIRQPDEQWRAIPGGACWNVARAAARLGVPTGYAGSVSTDVFGA 60

Query: 61  ELYALTQAAGLDMRFTQQYAKAPLLAMVTSTTPPDYFFIGDDSADLHFDVQRLPSGWLDE 120
           ELYALTQAAGLDMRFTQQYAKAPLLAMVTSTTPPDYFFIGDDSADLHFD QRLP+GWLD+
Sbjct: 61  ELYALTQAAGLDMRFTQQYAKAPLLAMVTSTTPPDYFFIGDDSADLHFDPQRLPAGWLDD 120

Query: 121 AQVLHFGCISLTREPLASRLLQIAQEAALRNKKIAFDPNWRKLMDSPAYGILLRRLLSIS 180
           AQVLHFGCISLTREPLASRLLQIAQEAA RNKKIAFDPNWRKLMDSP+Y +L ++LLS+S
Sbjct: 121 AQVLHFGCISLTREPLASRLLQIAQEAARRNKKIAFDPNWRKLMDSPSYRLLFQQLLSLS 180

Query: 181 HYVKVSDEDLERLFPGDSHALGTLQALAPQAEILYTLGAQGMQWIKGEQIIQQPAYRVEV 240
           HYVKVSDEDLERLFPGD++AL TLQ+LAPQAEILYTLGA+GMQWIKGEQ+I QPAYRVEV
Sbjct: 181 HYVKVSDEDLERLFPGDTNALATLQSLAPQAEILYTLGAKGMQWIKGEQVIDQPAYRVEV 240

Query: 241 IDTVGCGDASMGGWIASQLRQPQAPAQVHLQCAAAGAAIAASKAGAYAATWDEVQQMIAR 300
           IDTVGCGDASMGGWIASQLRQP APAQVHLQCAAAGAAIAASKAGAYAATWDEVQ +IA 
Sbjct: 241 IDTVGCGDASMGGWIASQLRQPDAPAQVHLQCAAAGAAIAASKAGAYAATWDEVQALIAH 300

Query: 301 A 301
           A
Sbjct: 301 A 301


>gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase
           [Herbaspirillum seropedicae SmR1]
          Length = 313

 Score = 55.1 bits (131), Expect = 7e-09,   Method: Compositional matrix adjust.
 Identities = 77/293 (26%), Positives = 116/293 (39%), Gaps = 35/293 (11%)

Query: 1   MTQSAAFVVFGEALTDFIRQPDGQWRAIP------GGACWNVARAAARLGVPTGYAGSVS 54
           MT     V +GEAL   +    G +  +        GA  NVA   ARLG+  G+A  V 
Sbjct: 1   MTMQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVG 60

Query: 55  TDVFGAELYALTQAAGL-------DMRFTQQYAKAPLLAMVTSTTPPDYFFIGDDSADLH 107
            D FG  +       G+       DM F        L A       P   +    SA  H
Sbjct: 61  NDAFGRFIRQRVAQEGVEVSRVITDMEFRTAI---QLKAKAVGGADPAIEYYRKGSAASH 117

Query: 108 FDVQRLPSGWLDEAQVLHFGCISLTREPLASRLLQIAQEAA--LR--NKKIAFDPNWRKL 163
             V    +G+   A+ LH   I+     L++  +  A +A   +R   K I+FDPN R +
Sbjct: 118 LSVDDFDAGYFGAARHLHATGIA---PALSATTMAFAHQAMDFMRGQGKTISFDPNLRPM 174

Query: 164 MDSPAYGILLRRLLSISHYVKVSDEDLERLFPG-------DSHALGTLQALAPQAEILYT 216
           +  P+  ++ ++L +++     +D  L  L  G       D+  +           ++  
Sbjct: 175 L-WPSQEVMAQQLNALAFK---ADWVLPGLSEGKILTGHDDAREIAGFYLERGVKLVVIK 230

Query: 217 LGAQGMQWIKGEQIIQQPAYRV-EVIDTVGCGDASMGGWIASQLRQPQAPAQV 268
           LGA+G  W  G+   +     V EV+DTVG GD    G I+  L     P  V
Sbjct: 231 LGAEGAYWRNGQGEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQAV 283


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.134    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 964,342
Number of extensions: 40809
Number of successful extensions: 163
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 163
Number of HSP's successfully gapped: 2
Length of query: 301
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 216
Effective length of database: 1,217,395
Effective search space: 262957320
Effective search space used: 262957320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 130 (54.7 bits)