BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0418.p (567 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion ... 541 e-155 gi|300310625|ref|YP_003774717.1| hemolysin activation translocat... 371 e-104 gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ... 62 9e-11 >gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 584 Score = 541 bits (1393), Expect = e-155, Method: Compositional matrix adjust. Identities = 273/523 (52%), Positives = 355/523 (67%), Gaps = 11/523 (2%) Query: 52 PDVNLGTPA-PNAVQQAFPEHEQPCRVISAITLEGDQAERFRFALDSVTGPQGAVGRCLG 110 PDV L PA P A +A P E PC + + L+G + RF + LD G GRCLG Sbjct: 66 PDVRL--PAQPGAAAEAVPAQESPCFAVHRVVLDGPGSARFDWLLDLPEANPG--GRCLG 121 Query: 111 SQGVNLVLAKVESAIVERGYTTTRVLARAQDLSTGQLRLTVVPGRVRHIRFAADADARGT 170 QG+N V++ +++AIV RGY TTR+LA+ QD+++G+LRLT+VPG++R IRFA +AD R T Sbjct: 122 VQGINAVMSALQNAIVARGYVTTRILAQPQDIASGELRLTIVPGKIRAIRFADNADPRAT 181 Query: 171 AFNALPISPGDILNLRDVEQGLENFKRVPTAQADIKITPSEDEGAQPGDSDLVIQYQQAF 230 +NALP SPGD+LNLRD+EQGLEN KRVPTA+AD++I PSE + A PGDSDLVI YQQ Sbjct: 182 QWNALPASPGDLLNLRDIEQGLENLKRVPTAEADVQIAPSEGKDAAPGDSDLVISYQQGI 241 Query: 231 PFRLSATLDDGGARSTGKYQVGLTLSYDNWWTLNDLFYVSVSRSL--NHYGDHGSHAYTL 288 P RL+ ++DD G ++TGKYQ T+S DNW TLNDLFY+S S L G GS + + Sbjct: 242 PIRLTLSVDDSGTKATGKYQGSATVSLDNWLTLNDLFYISFSHDLGGGDPGARGSQSQVV 301 Query: 289 HYSLPWGNWMAGVTLSGNRYHQTVSGAFENFVYSGRSETTEFKLSRLLHRDAAGKTTVFA 348 HYSLP G W T S NRYHQ V+G + ++YSG+SET E KLSRL++RDA KTT+ Sbjct: 302 HYSLPLGYWALAFTNSSNRYHQAVAGINQTYLYSGQSETNELKLSRLVYRDAVRKTTLSF 361 Query: 349 KGLVRTAYNAIDDQEVSAQQRRTTSWEAGVNQRQFLGEAIADATLAYRRSLDTQGSEPST 408 KG VR++ N +DD EV Q+RR WE G+ R+F+GE D LAY+R + + Sbjct: 362 KGFVRSSSNYVDDTEVQVQRRRVAGWEMGLAHREFIGEGTLDLALAYKRGTGAFDAMAAP 421 Query: 409 PFDLPQIATHYGLWLLDASLNAPFQL----GGGKLRYSGTLRAQWNRGNLPPQDQFSIGG 464 + + + L D +LNAPF L G +RY+ RAQWNR L PQD+FS+GG Sbjct: 422 EEAFGEGTSRFRLMTADLNLNAPFALPMPWGKQAMRYTLGARAQWNRTPLTPQDKFSLGG 481 Query: 465 RYTVRGFDGELTLQAERGWFLRNELALALGESGQELFAGVDTGAVSGPTAQFLTGQRLSG 524 RY+VRGFDGE L AERGW++RNELA ALG S QE++AG+D G V GP+ + L G+RL+G Sbjct: 482 RYSVRGFDGESNLLAERGWYVRNELAAALGASKQEIYAGLDYGEVGGPSTELLVGRRLAG 541 Query: 525 AALGLRGSLFKLSYEMFLATPLSKPQGFNTVALTGGFNLQWTF 567 A LGLRG+L + +YE+F P+S+P F T LT G +L W+F Sbjct: 542 AVLGLRGALGRTNYEVFAGVPISQPDRFKTSPLTAGMSLMWSF 584 >gi|300310625|ref|YP_003774717.1| hemolysin activation translocator protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 371 bits (953), Expect = e-104, Method: Compositional matrix adjust. Identities = 222/531 (41%), Positives = 304/531 (57%), Gaps = 21/531 (3%) Query: 47 QQESRPDVNLGTPAPNA-------VQQAFPEH-EQPCRVISAITLEGDQAERFRFALDSV 98 +Q S D+ T AP + V AFPE + R L Q + F F + Sbjct: 62 RQRSDEDLQSYTLAPESLCFPVQRVTLAFPEQVSEAVRRTGGFAL---QMDPFHFIQTYL 118 Query: 99 TGPQGAVGRCLGSQGVNLVLAKVESAIVERGYTTTRVLARAQDLSTGQLRLTVVPGRVRH 158 G G+ CLG QG+ +L +V I+ERGY+TTRV QDLSTGQL +T+VPG + Sbjct: 119 AGYAGS---CLGQQGIETILRRVTGQILERGYSTTRVGIGQQDLSTGQLSITLVPGIIHA 175 Query: 159 IRFAADADARGTAFNALPISPGDILNLRDVEQGLENFKRVPTAQADIKITPSEDEGAQPG 218 IRF D + +A P G +LNLRD+EQGLE KRV + D++I P++ PG Sbjct: 176 IRFK-DPAITASYRSAFPTQAGALLNLRDLEQGLEQMKRVASQDVDMQILPTD----VPG 230 Query: 219 DSDLVIQYQQAFPFRLSATLDDGGARSTGKYQVGLTLSYDNWWTLNDLFYVSVSRSLNHY 278 SD+V++ ++ P++L+ +LDD GA+STGKYQ G+ L+ D+ +NDLF S + Sbjct: 231 QSDVVLEVKRIKPWKLTLSLDDSGAKSTGKYQAGINLAIDDLLGINDLFNAGFSSDADRM 290 Query: 279 GD-HGSHAYTLHYSLPWGNWMAGVTLSGNRYHQTVSGAFENFVYSGRSETTEFKLSRLLH 337 G G+ YS P G W GV+ S +RYHQ ++G F+ FV SG SE E ++ +L Sbjct: 291 GQARGTTGNNFSYSAPAGYWTFGVSGSTSRYHQRIAGLFQTFVSSGHSENLEVRVQKLFQ 350 Query: 338 RDAAGKTTVFAKGLVRTAYNAIDDQEVSAQQRRTTSWEAGVNQRQFLGEAIADATLAYRR 397 RDA K ++ + R + + IDD E+ Q+R TT E G R + G+ DATLA R Sbjct: 351 RDAQQKNSLQLRIGKRWSRSYIDDTEIEVQRRNTTFAELGWMHRHYFGDVQLDATLANRW 410 Query: 398 SLDTQGSEPSTPFDLPQIATH-YGLWLLDASLNAPFQLGGGKLRYSGTLRAQWNRGNLPP 456 + P L T+ Y + +DA++ APF+LGG L Y GTLR Q +R L Sbjct: 411 GVSWFNGAADAPDRLSDGPTYRYSMQTIDATVVAPFKLGGQSLTYVGTLRGQHSRSRLYL 470 Query: 457 QDQFSIGGRYTVRGFDGELTLQAERGWFLRNELALALGESGQELFAGVDTGAVSGPTAQF 516 DQF+IG RYTVRGFDGELTL +E G FLRN+L +ALG +G L+AG+D G + GP+ Sbjct: 471 TDQFAIGSRYTVRGFDGELTLASESGVFLRNDLEIALGSTGAALYAGLDAGRLYGPSVAN 530 Query: 517 LTGQRLSGAALGLRGSLFKLSYEMFLATPLSKPQGFNTVALTGGFNLQWTF 567 L G L+GAA+GLRG++ L YE+F L +P+ F T GFNL + F Sbjct: 531 LQGDTLAGAAIGLRGAVSGLHYELFAGWALVRPEQFQTAMPAAGFNLIYQF 581 >gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 569 Score = 62.4 bits (150), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 97/427 (22%), Positives = 178/427 (41%), Gaps = 51/427 (11%) Query: 95 LDSVTGPQGAVGRCLGSQGVNLVLAKVESAIVERGYTTTRVLARAQDLSTGQLRLTVVPG 154 + + P GR G + L +E+ GYT +V Q+LS+G++R+ VV Sbjct: 71 VQAAVAPFSGQGRVYGD--IQQALEALENLYRSAGYTAVQVHVPEQELSSGEVRIDVVET 128 Query: 155 RVRHIRFAADAD-ARGTAFNALP-ISPGDILNLRDVEQGLENFKRVPTAQADIKITPSED 212 V ++ + + ++ ++P + G NLR + + ++ P Q ++ ++ Sbjct: 129 VVGKVQVSENRHFSQANIRASIPALKEGVPPNLRRISESVQLANDNPAKQVNVTLS---- 184 Query: 213 EGAQPGDS-DLVIQYQQAFPFRLSATLDDGGARSTGKYQVGLTLSYDNWWTLNDLFYVSV 271 AQ GD D I+ + P R+ LD+ G+ TG+++ G+ + + N + + + V+ Sbjct: 185 -AAQSGDQIDAKIEVKDDDPLRVMLNLDNTGSADTGRWRTGVAIQHSNLFNRDHVATVAY 243 Query: 272 SRSLNHYGDHGSHAYTLHYSLPWGNWMAGVTLSGNRYHQTVSGA-----FENFVYSGRSE 326 + S + Y+L Y LP + G +L +VS +GR E Sbjct: 244 TTSPDSPSGSKVDLYSLGYRLPL--YELGDSLDFIYGKSSVSSGQTLALNSTLNITGRGE 301 Query: 327 TTEFKLSRLLHRDA--AGKTTVFAKGLVRTAYNAIDDQEVSAQQRRTT----------SW 374 + + R+ + K + A + AI+ ++ T ++ Sbjct: 302 VYALRWNHFFARNGEWSSKLVLGADYKKVDSSCAINGGLLNGATLNTCLPYSTLPLSLTY 361 Query: 375 EAGVNQRQFLGEAIADATLAYRRSLDTQGSEPSTPFDLPQIATHYGLWLLD-ASLNAPFQ 433 A QRQ +GE I DA + R++ T + + D Q+ Y L+ S++ Sbjct: 362 SA---QRQGVGEVI-DANIGVARNMATGQARRN---DALQVTDRYSLFTNGRQSMDNFIA 414 Query: 434 LGGG-----------KLRYSGTLRAQWNRGNLPPQDQFSIGGRYTVRGFDGELTLQAERG 482 + GG ++R +G L Q + LP +QF + G VRGF E + A+ G Sbjct: 415 VRGGASWLKAFQNDWQMRLAGNL--QLSPHALPAVEQFGLAGATAVRGFT-ERAVSADSG 471 Query: 483 WFLRNEL 489 L +EL Sbjct: 472 VVLNSEL 478 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,763,840 Number of extensions: 75622 Number of successful extensions: 211 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 3 Length of query: 567 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 477 Effective length of database: 1,193,720 Effective search space: 569404440 Effective search space used: 569404440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)