BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0339.p (358 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspiri... 107 2e-24 gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum... 105 8e-24 gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspiri... 100 2e-22 gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspiri... 81 2e-16 gi|300311313|ref|YP_003775405.1| acyltransferase [Herbaspirillum... 80 2e-16 >gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 662 Score = 107 bits (267), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 115/369 (31%), Positives = 180/369 (48%), Gaps = 50/369 (13%) Query: 10 TAGAAR--FSGYRPWLDGVRAVAILLVLLEHTNLFKQYNLGG-VGVGIFFALSGYLITGL 66 +AGAA YRP +DG+RAVA+ V++ H F ++ GG +GV IFF +SG+LI+ + Sbjct: 8 SAGAAPSLHPAYRPDIDGLRAVAVGSVVIFHA--FPEWFAGGFIGVDIFFVISGFLISLI 65 Query: 67 LWDEFAREGRLALVPFYIRRFARLAPALLLV---ALIAGLAFLLEGQRRAAFNAIFA-VT 122 L+ GR ++V FY+RR R+ PAL+ V L+ G L + + +F T Sbjct: 66 LFKNL-EHGRFSIVDFYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLFGGST 124 Query: 123 YTTNY-----AAIFSGH-HLAGFGHTWSLAVEEHFYLIWPPLMLLLMRRIPAASFVKAVL 176 + +N+ + F H WSLA+EE FYL WP L+ + +R F+ + Sbjct: 125 FISNFLFWRESGYFDNSAETKPLLHLWSLAIEEQFYLFWPLLLAFVWKR--RWGFLGIMG 182 Query: 177 AIAVMVLCWRAFLFGVFGEQVRLMLYAGSIERLDSLLYGCAAAV------AVRQGWRPGN 230 I ++ + G + + + R L+ G A A + +G++ Sbjct: 183 VIGLLSFGVNLYFIG----HDQSAAFYSPLARFWELMIGGALAYINLHRQDLTRGYKNLQ 238 Query: 231 GAMAAGVVALAVSVL--------WIAPLSSVLGPTLIGAG-GALFIAAIDCERRAVLQQV 281 + A ++ ++L W A L S+ L+ AG GA F RR V Sbjct: 239 AWLGAALLVAGFALLDGARAFPGWWALLPSLGAFLLLSAGPGAWF------NRR-----V 287 Query: 282 LSTKLMVRTGILSYGIYLWHIPLLQLSSKNGEGSLPSI--LAAMALAVILAHLSYRYCEV 339 L++K MV G++SY +YLWH PLL ++ S P+ AA+ A++L+ L+YRY E Sbjct: 288 LASKGMVALGLVSYPLYLWHWPLLAFATIIEGESAPAEWRAAAVLGALLLSWLTYRYIER 347 Query: 340 PIRDSVRQR 348 P+R S R R Sbjct: 348 PLRGSTRSR 356 >gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum seropedicae SmR1] Length = 656 Score = 105 bits (261), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 120/354 (33%), Positives = 184/354 (51%), Gaps = 30/354 (8%) Query: 8 GSTAGAARFSGYRPWLDGVRAVAILLVLLEHTNLFKQYNLGG-VGVGIFFALSGYLITGL 66 G+ + A GYRP +DG+RA+A+L V++ H F ++ GG VGV IFF +SG+LI+G+ Sbjct: 11 GAASPATAHPGYRPDIDGLRAIAVLSVVVFHA--FPEWLPGGFVGVDIFFVISGFLISGI 68 Query: 67 LWDEFAREGRLALVPFYIRRFARLAPALLLVALIAGLA----FLLEGQRRAAFNAIFAVT 122 L+D R+ R ++V FYIRR R+ PALLLV L LA L E R+ + + Sbjct: 69 LFDALQRD-RFSIVDFYIRRIRRIFPALLLVMLACLLAGWFILLPEEYRQLGKHLLGGAG 127 Query: 123 YTTN-----YAAIF-SGHHLAGFGHTWSLAVEEHFYLIWPPLMLLLMRRIPAASFVKAVL 176 + +N A F + H WSL VEE FYL WP ++ RR + +L Sbjct: 128 FVSNIVLWNEAGYFDTSAETKPLLHLWSLGVEEQFYLFWPLMLWAAWRR--RTGMLAVLL 185 Query: 177 AIAVMVLCWRAFLFGVFGEQVRLMLYAGSIERLDSLLYGCAAAVAVRQGWRP----GNGA 232 + + W V+ + + + + R L G A RQG P + Sbjct: 186 GVMALSFGWNI----VWLHRDAVAAFYLPMSRFWELGAGALLAWRHRQGAVPTSVQAHRL 241 Query: 233 MAAGVVALAVSVLWIAPLSSVLG-PTLIGAGGALFIAAIDCERRAVLQQVLSTKLMVRTG 291 AG++ + V++ I + G L+ GA+ + A + R + + +LS + MV G Sbjct: 242 SVAGLLLIVVAIATITKDDAFPGWWALLPVTGAVLLLAAGPQAR-INRSLLSHRTMVWVG 300 Query: 292 ILSYGIYLWHIPLLQLSSKNGEGSLPSI---LAAMALAVILAHLSYRYCEVPIR 342 ++SY +YLWH PLL ++ G+ PS+ LAA+ALA++LA L+YR E+P+R Sbjct: 301 LISYPLYLWHWPLLAF-ARLVVGATPSVQLRLAAVALALLLAWLTYRLVELPVR 353 >gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 681 Score = 100 bits (248), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 118/369 (31%), Positives = 171/369 (46%), Gaps = 71/369 (19%) Query: 18 GYRPWLDGVRAVAILLVLLEHTNLFKQYNLGG-VGVGIFFALSGYLITGLLWDEFAREGR 76 YRP +DG+RA+AI+ V++ H F GG +GV IFF +SG+LI+ +++ + +GR Sbjct: 31 SYRPDIDGLRAIAIVSVVVFHA--FPDAMPGGFIGVDIFFVISGFLISSIIFRQL-DQGR 87 Query: 77 LALVPFYIRRFARLAPALLLV---ALIAGLAFLLEGQRR---------AAFNAIFAVTYT 124 + FY+ R R+ PAL+LV + G LL + R A F + Sbjct: 88 FSFTDFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGAGFIQNLVLWKE 147 Query: 125 TNYAAIFSGHHLAGFGHTWSLAVEEHFYLIWPPLMLLLMR-RIP-AASFVKAVLAIAVMV 182 + Y + S + H WSL+VEE FYL++P L+ L R R+P S V +LA + Sbjct: 148 SGYFDVESD--MKPLLHLWSLSVEEQFYLVYPLLVCGLWRARMPLRNSLVLLMLASFALN 205 Query: 183 LCWRAFLFGVFGEQVRLMLYAGSIERLDSLLYGCAAAVAV-RQGWRPGNGAMAAGVVALA 241 L W A Q + + R+ LL G A V R+G VA + Sbjct: 206 LWWVA--------QDPVKTFYLPFTRVWELLAGALCAFGVMRKG------------VAFS 245 Query: 242 VSVLWIAPLSSV-LGPTLIGAGGALFIAAIDCERR------------AVL---------- 278 S + L S+ L L A + ID R AVL Sbjct: 246 TSSFRLPHLPSIHLRSVLGLALLLCGLVLIDRHRYFPGAWALLPVTGAVLLVSSGPQAWV 305 Query: 279 -QQVLSTKLMVRTGILSYGIYLWHIPLLQ----LSSKNGEGSLPSILAAMALAVILAHLS 333 + +LS L+V G++SY +YLWH P+L L+ LP LAA+AL+V+LA L+ Sbjct: 306 NRHLLSRPLLVWIGLVSYLLYLWHWPILSFMHILTPTRPGAPLP--LAAVALSVLLAWLT 363 Query: 334 YRYCEVPIR 342 YR E P+R Sbjct: 364 YRLLERPVR 372 >gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 680 Score = 80.9 bits (198), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 56/162 (34%), Positives = 82/162 (50%), Gaps = 18/162 (11%) Query: 17 SGYRPWLDGVRAVAILLVLLEHTNLFKQYNLGG-VGVGIFFALSGYLITGLLWDEFAREG 75 S YR +DG+RA+A+L V+ H F + GG +GV +FF +SGYLI+ ++++ R G Sbjct: 12 SKYRSDIDGLRAIAVLAVVSFHG--FPNFVRGGFIGVDVFFVISGYLISTIIFENLDR-G 68 Query: 76 RLALVPFYIRRFARLAPALLLV---ALIAGLAFLLEGQRR---------AAFNAIFAVTY 123 FY RR R+ PALLLV + G LL + + A F + F + Sbjct: 69 TFTFTEFYARRVRRIFPALLLVLSTCFVVGWFVLLTDEYKQLGKHIAGGAGFVSNFLLRD 128 Query: 124 TTNYAAIFSGHHLAGFGHTWSLAVEEHFYLIWPPLMLLLMRR 165 + Y + H WSL +EE FY++WP L+ RR Sbjct: 129 ESGYFD--NSAETKPLLHLWSLGIEEQFYILWPMLLFFSWRR 168 >gi|300311313|ref|YP_003775405.1| acyltransferase [Herbaspirillum seropedicae SmR1] Length = 350 Score = 80.5 bits (197), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 106/307 (34%), Positives = 147/307 (47%), Gaps = 30/307 (9%) Query: 21 PWLDGVRAVAILLVLLEHTNLFKQYNLGGVGVGIFFALSGYLITGLLWDEFAREGRLALV 80 P LDG RA+AI +V L H L + GG+GV IFF LSGYLIT LL EF R + + Sbjct: 10 PSLDGWRALAIAIVFLSHAGL-GRIVPGGLGVTIFFFLSGYLITTLLVREFDRSDSVNVR 68 Query: 81 PFYIRRFARLAPALLLVALIAGLAF---LLEGQRRAAFNAIFA-VTYTTNYAAIF--SGH 134 FY+RR RL P LLLV ++ + G A+++ A + Y NY +F GH Sbjct: 69 HFYLRRLLRLTPPLLLVLVLTYTLTWLGIFTGD--ASWSGFLAQLFYFANYYVLFFDPGH 126 Query: 135 HL-AGFGHTWSLAVEEHFYLIWPPLMLLLMRRIPAASFVKAVLAIAVMVLCWRAFLFGVF 193 L G G WSLAVEEHFY ++P L + LM + + + ++VL WR L + Sbjct: 127 TLPQGTGVFWSLAVEEHFYFVFPALFIFLMHKADRRKIPLYLGGLCLLVLLWRYGLV-LL 185 Query: 194 GEQVRLMLYAGSIERLDSLLYGCAAAV--------------AVRQGWRPGNGAMAAGVVA 239 G+ Y + R+DS+L+GC A AVR G + +A Sbjct: 186 GDASMDRTYYATDTRIDSILFGCILATLKLPPAVYAALHKPAVRVGGVTLGVLLLLLSLA 245 Query: 240 LAVSVLWIAPLSSVLGPTLIGAGGALFIAAIDCERRAVLQQVLSTKLMVRTGILSYGIYL 299 + V SV G L+ LF ++ + ++L+ + + R GI SY IYL Sbjct: 246 VRDEVFRETLRYSVQGLALM----PLFFYSV-AHPHSFPFRLLNLRWVQRIGIYSYSIYL 300 Query: 300 WHIPLLQ 306 H LL Sbjct: 301 SHEILLS 307 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.141 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 872,401 Number of extensions: 28115 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 6 Length of query: 358 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 272 Effective length of database: 1,212,660 Effective search space: 329843520 Effective search space used: 329843520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)