BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0331.p (429 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 807 0.0 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 112 7e-26 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 108 7e-25 gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 97 2e-21 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 807 bits (2085), Expect = 0.0, Method: Compositional matrix adjust. Identities = 395/422 (93%), Positives = 412/422 (97%) Query: 1 LDTVPIWVQALALLILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTE 60 ++TVPIWVQ LAL ILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTE Sbjct: 1 METVPIWVQVLALFILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTE 60 Query: 61 RLLSLILIANTVINALATALVTALAIAAFGNHQRVITAATAFVAALLIVFAEIVPKVIGA 120 RLLSL+LIANT INALATALVTALAIAAFGNHQRVITAATA VAALLI+FAEIVPKVIGA Sbjct: 61 RLLSLVLIANTAINALATALVTALAIAAFGNHQRVITAATACVAALLIIFAEIVPKVIGA 120 Query: 121 THPERIALFTSFILKPLMRVAKPLVWFVNIFVSAILKLLHIKTGARAAEQRISPEELRSI 180 T+PERI+LFT FILKPLMRVAKPL+WFVN+FVSAIL+LL IKTGARAAEQRISPEELRSI Sbjct: 121 TYPERISLFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPEELRSI 180 Query: 181 VLEGGSFIPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLAAPVEDILHQLATCYHNK 240 VLEGGSFIPQKHKSILLNLFDLEKISVEDVMTPR+QVEALNLAAPV+DILHQLATCYHNK Sbjct: 181 VLEGGSFIPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYHNK 240 Query: 241 LPVFEGEINHIVGILHVRKTVSLFRQEEEISHDDIRALLSAPYYVPVETDIFTQLQYFQE 300 LPVFEGEINHIVGILHVRKTVSLFR+EEEISHDDIR LLSAPYYVPVETD+FTQLQYFQE Sbjct: 241 LPVFEGEINHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYFQE 300 Query: 301 NHERLGIVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFNWDKEGTCLLEGSTTL 360 NHERLG+VVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGF WDK+G CLLEGSTTL Sbjct: 301 NHERLGVVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDKDGACLLEGSTTL 360 Query: 361 RDINKRLGLKLPLDGPKTINGLLLETLQDIPEASVAVKIDGCVIEIVQVQNQAIRMVKLI 420 RDINKRLGL+LPLDGPKTINGLLLE LQDIPEASVAVK+DGCVIEIVQVQNQAIRMVKLI Sbjct: 361 RDINKRLGLQLPLDGPKTINGLLLEYLQDIPEASVAVKMDGCVIEIVQVQNQAIRMVKLI 420 Query: 421 AL 422 AL Sbjct: 421 AL 422 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 112 bits (279), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 105/418 (25%), Positives = 195/418 (46%), Gaps = 37/418 (8%) Query: 20 SAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTERLLSLILIANTVINALATA 79 SAF S E ++ A+ + RL ++K+G A +L L + +++ I +NA+A Sbjct: 17 SAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFFTVVQIG---LNAVAI- 72 Query: 80 LVTALAIAAFGNHQRVITA-------------ATAF--VAALLIVFAEIVPKVIGATHPE 124 L + A H R I + A +F V A I+FA+++PK + PE Sbjct: 73 LGGVVGEPALTPHMREIISSFYIGPWLENASFAMSFLVVTAFFILFADLMPKRLAMVAPE 132 Query: 125 RIALFTSFILKPLM---RVAKPLVWFVNIFVSAILKLLHIKTGARAAEQRISPEELRSIV 181 R A+ ++KP++ + KPLVWF N I L + + ++ I+P E+ ++V Sbjct: 133 RAAVA---LVKPMLLLETLFKPLVWFFNSLSKWIFTLFGL---PQVRQEVITPNEIHAMV 186 Query: 182 LEG---GSFIPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLAAPVEDILHQLATCYH 238 G G + Q+H ++ N+F+LE V MTPR + L I ++ H Sbjct: 187 DAGAQAGVLLTQEHH-LIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPH 245 Query: 239 NKLPVFEGEINHIVGILHVRKTVSLFRQEEEISHDDIRALLSAPYYVPVETDIFTQLQYF 298 ++ V +G I+ ++G + + + + E+ S D + L+ P +P +++ L+ Sbjct: 246 SRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPLSIPDSLNLWEVLERM 304 Query: 299 QENHERLGIVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFNWDKEGTCLLEGST 358 + E L IVV+EY V G+++ D+ ++G + + E + L++G T Sbjct: 305 KAMDEDLAIVVNEYALVVGIISTTDVTGVLMGNLLAIT--EEEQQIVRRDENSWLMDGLT 362 Query: 359 TLRDINKRLGLKLPLD--GPKTINGLLLETLQDIPEASVAVKIDGCVIEIVQVQNQAI 414 L D+ L + + G +T++G ++ L+ IP+ + V E+V ++ I Sbjct: 363 PLNDVMSELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRI 420 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 108 bits (271), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 60/247 (24%), Positives = 121/247 (48%), Gaps = 21/247 (8%) Query: 194 SILLNLFDLEKISVEDVMTPRAQVEALNLAAPVEDILHQLATCYHNKLPVFEGEINHIVG 253 S++ +F + ++V D+M PR+Q++ ++++ P+E + ++ H++ P +GE + +VG Sbjct: 55 SMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDKVVG 114 Query: 254 ILHVRKTVSLFRQEEEISHDDIRALLSAPYYVPVETDIFTQLQYFQENHERLGIVVDEYG 313 IL + + + +E D+R +L ++P + L+ F+ N + +VVDEYG Sbjct: 115 ILLAKDLLRYYAEESF----DVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYG 170 Query: 314 EVQGLVTLEDIIEEMIGEFTTSSPGSSGE-----------GFNWDKEGTCLLEGSTTLRD 362 V GL+T+ED++E+++G+ E G W ++ T + Sbjct: 171 GVAGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARW------RVKALTEIEQ 224 Query: 363 INKRLGLKLPLDGPKTINGLLLETLQDIPEASVAVKIDGCVIEIVQVQNQAIRMVKLIAL 422 N+ +G + D TI GL+ + +P IDG E+++ + + ++ + L Sbjct: 225 FNEEVGSRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLVSQL 284 Query: 423 PPPTTPD 429 PP D Sbjct: 285 PPAPASD 291 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 97.4 bits (241), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 90/427 (21%), Positives = 192/427 (44%), Gaps = 27/427 (6%) Query: 14 LILILLSAFFSMTETALMAANRHRLRHLAK-QGNRAAITILWLLDRTERLLSLILIANTV 72 L+ L+ FF E +L+ + R+R +AK QG R I + + ++ + LS + T+ Sbjct: 11 FFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAV-VHNQLDAYLSACQLGITL 69 Query: 73 IN---------ALATALVTALAIAAFGNHQRVITAATAF----VAALLIVFAEIVPKVIG 119 + A A L ++A N + + + F ++ L IV E+ PK + Sbjct: 70 ASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVAGELAPKSMA 129 Query: 120 ATHPERIALFTSFILKPLMRVAKPLVWFVNIFVSAILKLLHIKTGARAAEQRISPEELRS 179 PE++ L+ + L PL+W +N + +L++ + G + S +EL+ Sbjct: 130 IRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAG-HGHDAHYSSDELKL 188 Query: 180 IVLEG------GSFIPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLAAPVEDILHQL 233 I+ G G F + ++L + ++ V D+M P +++ AL +E+ L + Sbjct: 189 ILRAGNKSGKNGKFT-RDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEENLDII 247 Query: 234 ATCYHNKLPVFEGEINHIVGILHVRKTVSLFRQEEEISHDDIRALLSAPYYVPVETDIFT 293 +++ P ++ E ++G++H++ Q++ + +++ L Y+ Sbjct: 248 YRNRYSRYPYYDAERQQVLGLVHLKDV--FLAQQDGRAIANLKDYLRPVQYISPALPALD 305 Query: 294 QLQYFQENHERLGIVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFNWDKEGTCL 353 L+ F+ ++ + G +TL++++ ++GE ++GE D +GT L Sbjct: 306 LLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQD-DGTLL 364 Query: 354 LEGSTTLRDINKRLGLKLPLDGP-KTINGLLLETLQDIPEASVAVKIDGCVIEIVQVQNQ 412 +GS + + LG+ + D ++ GL++E L D+P+ + + I ++ Sbjct: 365 GKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKRMSGP 424 Query: 413 AIRMVKL 419 I +VK+ Sbjct: 425 KIVLVKV 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,180,680 Number of extensions: 44934 Number of successful extensions: 132 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 125 Number of HSP's successfully gapped: 4 Length of query: 429 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 341 Effective length of database: 1,203,190 Effective search space: 410287790 Effective search space used: 410287790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)