BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0319.p (325 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptid... 572 e-164 gi|300309747|ref|YP_003773839.1| membrane metalloendopeptidase [... 77 2e-15 gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase... 67 2e-12 gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Her... 66 4e-12 gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillu... 57 3e-09 >gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptidase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 572 bits (1473), Expect = e-164, Method: Compositional matrix adjust. Identities = 294/325 (90%), Positives = 309/325 (95%) Query: 1 MQIILLHPRFTKARSITLGTKHILLLLLLMLTMVAGSSMGITYFLLNRAGNPDASPMLHK 60 MQIILLHPRFTKARSITLGT+H+LLLLLL++ V G SM I+Y +L +AG+P+ASPMLHK Sbjct: 1 MQIILLHPRFTKARSITLGTRHLLLLLLLLIGSVIGCSMLISYLMLRQAGDPEASPMLHK 60 Query: 61 LAVSMSQEEDGRNEKYLKENLAMMAVKVGEMQAQMMRLDALGERVQGLAGIRPEEFNFKE 120 LA SMSQEED R EK++KENLAMMAVKVGEMQAQMMRLDALGERVQGLAGIRPEEFNFKE Sbjct: 61 LAASMSQEEDDRKEKFVKENLAMMAVKVGEMQAQMMRLDALGERVQGLAGIRPEEFNFKE 120 Query: 121 APGRGGAEVSSQAGPARDVSMDELRRMLDSLAVDAGHRADYLNAVESTLMGDKIKSRLLP 180 PGRGGAEVSS AGP RDV +DELRRMLD+LAVDAGHR DYLNAVESTLMGDKIKSRLLP Sbjct: 121 LPGRGGAEVSSLAGPGRDVGLDELRRMLDNLAVDAGHRTDYLNAVESTLMGDKIKSRLLP 180 Query: 181 TNQPVNVAYNSSSFGWRLDPFTGHNAFHEGLDFPAPVGTRIVAAAAGVVIASEYHYQFGN 240 TNQPVNVAYNSSSFGWRLDPFTGHNAFHEGLDFPAPVGTRIVAAAAGVVIASEYHYQFGN Sbjct: 181 TNQPVNVAYNSSSFGWRLDPFTGHNAFHEGLDFPAPVGTRIVAAAAGVVIASEYHYQFGN 240 Query: 241 MLEIDHGNNIITRYAHASRLYVKVGDIVKRGQHVADVGSTGRSTGAHLHFEVRIRGIAQD 300 MLEIDHGNNIITRYAHASRL+VKVGDIVKRGQHVADVGSTGRSTGAHLHFEVRIRGIAQD Sbjct: 241 MLEIDHGNNIITRYAHASRLFVKVGDIVKRGQHVADVGSTGRSTGAHLHFEVRIRGIAQD 300 Query: 301 PRKFLALGATGGNQPKQLPVFVAGR 325 PRKFLALGA GGNQPKQ+PVFVAGR Sbjct: 301 PRKFLALGANGGNQPKQMPVFVAGR 325 >gi|300309747|ref|YP_003773839.1| membrane metalloendopeptidase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 77.0 bits (188), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 50/133 (37%), Positives = 66/133 (49%), Gaps = 4/133 (3%) Query: 171 GDKIKSRLLPTNQPVNVAYNSSSFGWRLDPFTGHNAFHEGLDFPAPVGTRIVAAAAGVVI 230 G +K L + P+ SS F RL P G H G+DF AP GT I AAA GVV Sbjct: 329 GKSLKKAFLKS--PLAFTRISSGFSMRLHPILGKWKQHTGVDFAAPTGTPIHAAADGVVD 386 Query: 231 ASEYHYQFGNMLEIDHGNNIITRYAHASRLY--VKVGDIVKRGQHVADVGSTGRSTGAHL 288 +GN++ I H + T Y H SR +K G V + + VG TG +TG HL Sbjct: 387 FVGKQNGYGNIVVIKHWSGYSTAYGHMSRFAPDIKKGMKVSQNDVIGFVGMTGWATGPHL 446 Query: 289 HFEVRIRGIAQDP 301 H+E R+ ++P Sbjct: 447 HYEFRVNNQPRNP 459 >gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase [Herbaspirillum seropedicae SmR1] Length = 470 Score = 67.0 bits (162), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 38/114 (33%), Positives = 63/114 (55%), Gaps = 4/114 (3%) Query: 192 SSFGWRLDPFTGHNAFHEGLDFPAPVGTRIVAAAAGVVIASEYHYQFGNMLEIDHGNNII 251 + FG R G GL A G + A AAG V+ +++ FGN++ +DHGN + Sbjct: 358 AKFGSR----RGDGPTSRGLFIRAHEGAEVRAVAAGRVVFADWLRGFGNLIIVDHGNQYM 413 Query: 252 TRYAHASRLYVKVGDIVKRGQHVADVGSTGRSTGAHLHFEVRIRGIAQDPRKFL 305 T Y + + + GD+VK G +A G++G + + L+FE+R +G A DP+ ++ Sbjct: 414 TIYGNNQSVLKRAGDLVKAGDTIATAGNSGGNEQSGLYFEMRYQGRAFDPQGWV 467 >gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 65.9 bits (159), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 36/99 (36%), Positives = 57/99 (57%), Gaps = 2/99 (2%) Query: 208 HEGLDFPAPVGTRIVAAAAG-VVIASEYHYQFGNMLEIDHGNNIITRYAHASRLYVKVGD 266 +G+D G ++AA AG V+ A +GN++ I H +N+++ YAH + VK G Sbjct: 232 KKGVDIAGKAGQAVLAAGAGKVMYAGSGIRGYGNLVIIKHTSNLLSAYAHNQTILVKEGQ 291 Query: 267 IVKRGQHVADVGSTGRSTGAHLHFEVRIRGIAQDPRKFL 305 V +GQ +A++G + T LHFE+R +G DP K+L Sbjct: 292 SVTKGQKIAEMGKSDSDT-VKLHFEIRQQGKPVDPSKYL 329 >gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillum seropedicae SmR1] Length = 254 Score = 56.6 bits (135), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 32/93 (34%), Positives = 54/93 (58%), Gaps = 2/93 (2%) Query: 205 NAFHEGLDFPAPVGTRIVAAAAGVVIASEYHYQ-FGNMLEIDHGNNIITRYAHASRLYVK 263 +++ +G+D G +VAAAAG V + + + +GNM+ I H +++ YAH + VK Sbjct: 151 DSYKKGIDIIGSEGQSVVAAAAGKVTYAGHGIRGYGNMVIIKHTPQLLSVYAHNKTILVK 210 Query: 264 VGDIVKRGQHVADVGSTGRSTGAHLHFEVRIRG 296 G V RGQ +A +G++ + L+FE+R G Sbjct: 211 EGQTVTRGQQIATMGNSD-TNKVKLYFEIRRNG 242 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 988,458 Number of extensions: 39765 Number of successful extensions: 128 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 126 Number of HSP's successfully gapped: 5 Length of query: 325 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 240 Effective length of database: 1,217,395 Effective search space: 292174800 Effective search space used: 292174800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)