BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0303.p (592 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309672|ref|YP_003773764.1| cell division FtsI protein/peni... 1185 0.0 gi|300313947|ref|YP_003778039.1| cell division protein FtsI [Her... 132 1e-31 gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidogl... 57 4e-09 >gi|300309672|ref|YP_003773764.1| cell division FtsI protein/penicillin-binding protein 2 [Herbaspirillum seropedicae SmR1] Length = 592 Score = 1185 bits (3066), Expect = 0.0, Method: Compositional matrix adjust. Identities = 581/592 (98%), Positives = 587/592 (99%) Query: 1 MTKMPPRTNLAGGRVAASKGVAFSSNPILQVKLPAWRSRFVLFVLFAAFVALVLRALWLQ 60 MTKMPPRTNL+GGRVAASKGVAFSSNPILQVKLPAWRSRFVLFVLF AFVALVLRALWLQ Sbjct: 1 MTKMPPRTNLSGGRVAASKGVAFSSNPILQVKLPAWRSRFVLFVLFVAFVALVLRALWLQ 60 Query: 61 GVSTDFLQKQGASRYARTLEMPATRGKITDRNGQVLASSVPVKAIWAIPEDVQAAPKDKL 120 GVSTDFLQKQGASRYARTLE+PATRGKITDRNGQVLASSVPVKAIWAIPEDVQAAPKDKL Sbjct: 61 GVSTDFLQKQGASRYARTLELPATRGKITDRNGQVLASSVPVKAIWAIPEDVQAAPKDKL 120 Query: 121 VQLAKLLEMSDAELRKKLDSDRSFVYLKRQVEQDTADKIVKLGIPGIETRKEYKRFYPEG 180 VQLAKLLEMSDA+LRKKLDSDRSFVYLKRQVEQDTADKIVKLGIPGIETRKEYKRFYPEG Sbjct: 121 VQLAKLLEMSDADLRKKLDSDRSFVYLKRQVEQDTADKIVKLGIPGIETRKEYKRFYPEG 180 Query: 181 EVMAHVVGFTNIEDAGQDGMELAAQKTLAGVTGSRRVIKDRLGRVVEDIESIREPHDGRD 240 EVMAHVVGFTNIEDAGQDGMELAAQKTLAGVTGSRRVIKDRLGRVVEDIESIREPHDGRD Sbjct: 181 EVMAHVVGFTNIEDAGQDGMELAAQKTLAGVTGSRRVIKDRLGRVVEDIESIREPHDGRD 240 Query: 241 LTLSIDSKIQYIAFTQLKEAVEKYKAKAGGIIVVDAKTGEVLALANMPTYNPNDRSELTG 300 LTLSIDSKIQYIAFTQLKEAVEK+KAKAGGIIVVDAKTGEVLALAN+PTYNPNDRS LTG Sbjct: 241 LTLSIDSKIQYIAFTQLKEAVEKFKAKAGGIIVVDAKTGEVLALANLPTYNPNDRSVLTG 300 Query: 301 AQLRNRVVTDTFEPGSTLKPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSHAHGA 360 AQLRNRVVTDTFEPGST+KPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSHAHG Sbjct: 301 AQLRNRVVTDTFEPGSTMKPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSHAHGP 360 Query: 361 LTVAQIIEKSSNIGTAKIALGMPAEEMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWRP 420 LTVAQIIEKSSNIGTAKIALGMP EEMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWRP Sbjct: 361 LTVAQIIEKSSNIGTAKIALGMPPEEMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWRP 420 Query: 421 IEQATMSYGHGISVSLIQLAHAYLIFARNGDIIPLSFTKVTDSPIGQRVISADTALQMRR 480 IEQATMSYGHGISVSLIQLAHAYLIFARNGDIIPLSFTKVTDSPIGQRVISADTALQMRR Sbjct: 421 IEQATMSYGHGISVSLIQLAHAYLIFARNGDIIPLSFTKVTDSPIGQRVISADTALQMRR 480 Query: 481 MLETVVAPGGTAPQAQVPGYRVGGKTGTAYKIENGKYVRKYVASFSGIAPMSDPRLIISV 540 MLETVVAPGGTAPQAQVPGYRVGGKTGTAYKIE GKYVRKYVASFSGIAPMSDPRLIISV Sbjct: 481 MLETVVAPGGTAPQAQVPGYRVGGKTGTAYKIEGGKYVRKYVASFSGIAPMSDPRLIISV 540 Query: 541 MIDEPQGQHYGGPVAGPVFANVAANALRALNVPPDSSVTNIIIPKEPLEESM 592 MIDEPQGQHYGGPVAGPVFANVAANALRALNVPPDSSVTNIIIPKEPLEESM Sbjct: 541 MIDEPQGQHYGGPVAGPVFANVAANALRALNVPPDSSVTNIIIPKEPLEESM 592 >gi|300313947|ref|YP_003778039.1| cell division protein FtsI [Herbaspirillum seropedicae SmR1] Length = 656 Score = 132 bits (331), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 150/594 (25%), Positives = 237/594 (39%), Gaps = 101/594 (17%) Query: 48 AFVALVLRALWLQGVSTDFLQKQGASRYARTLEMPATRGKITDRNGQVLASSVPVKAIWA 107 F L+ R LWLQ V D Q + + RG I DRNG VLA + + Sbjct: 28 CFSLLLARFLWLQVVRHDAYAAQAEENRISVVPIVPNRGLILDRNGVVLARNYSAYTLEI 87 Query: 108 IPEDVQAAPKDKLVQLAKLLEMS------DAELRKKLDSDRSFVYLKRQVEQDTADKIV- 160 P ++ D + +L L+E+ L+++ + S R +++ A Sbjct: 88 TPSKIRTNIDDLIDELGTLVEIQPRDRRRFRRLQEESKNFESVPIRTRLTDEEVAKFTAQ 147 Query: 161 KLGIPGIETRKEYKRFYPEGEVMAHVVGF---------TNIEDA------------GQDG 199 + PG+E + R YP G+ AHV+G+ I D+ G++G Sbjct: 148 RYRFPGVEVQARLFRQYPYGKTAAHVIGYIGRISQRDAERIADSDDEANYNGTDYIGKEG 207 Query: 200 MELAAQKTLAGVTGSRRVIKDRLGRVVEDIESIREPHD-GRDLTLSIDSKIQYIAFTQLK 258 +E + + L G TG V GR V + R P G++L LSID ++Q K Sbjct: 208 LEKSYESQLHGTTGYEEVEISASGRAVRSLS--RTPATPGKNLILSIDIELQ-------K 258 Query: 259 EAVEKYKAKAGGIIVVDAKTGEVLALANMPTYNPNDRSELTGAQ------------LRNR 306 E + + G ++ ++ TG++LA + P+Y+PN E Q L NR Sbjct: 259 VVEEAFGDQKGALVAINPATGDILAYVSQPSYDPNLFVEGIDQQSWDELNKSPDRPLLNR 318 Query: 307 VVTDTFEPGSTLKPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSH--AHGALTVA 364 ++ + PGST KPF AL+ + P + PG +G D HG + + Sbjct: 319 PLSGLYSPGSTYKPFMALAALELGKRRPQDAIRD-PGYFILGNHRFRDDKEGGHGVVDMY 377 Query: 365 QIIEKSSNIGTAKIALGMPAEEMWEMFTTVGFGQ-------QPKFG-FPGA----VAGRV 412 + + S + +A M + + GFGQ K G P A R Sbjct: 378 RSVVVSCDTYYYMLANDMGVNAIHDFMKPFGFGQITGIDLEHEKRGILPSTEWKRTAFRK 437 Query: 413 RPYKSWRPIEQATMSYGHGI-SVSLIQLAHAYLIFARNGDIIPLSFTKVTDS-------- 463 K W + ++ G G S + +QLA A I A NG ++ K+ + Sbjct: 438 PEQKRWIAGDTVSLGIGQGYNSFTPLQLAQATAILANNGVVMKPHLVKIVEDGVTHERTE 497 Query: 464 ---------PIGQRVISADTALQMRRMLETVVAPGGTAPQAQVPGYRVGGKTGTAYKI-- 512 P+ Q I ++R + VV G A + Y GGKTGTA + Sbjct: 498 TVPKESYRIPMKQENIDF-----IKRAMVGVVKEGTGAAAFRGAPYESGGKTGTAQVVNI 552 Query: 513 -ENGKYVRKYVAS-------FSGIAPMSDPRLIISVMIDEPQGQHYGGPVAGPV 558 +N KY K ++ + G AP PR+ I+ ++ + +G VA P+ Sbjct: 553 AKNQKYDSKKLSRIYHDNGLYIGFAPADAPRIAIAAVV---ENGGWGAGVAAPL 603 >gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 771 Score = 57.0 bits (136), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 79/321 (24%), Positives = 131/321 (40%), Gaps = 63/321 (19%) Query: 271 IIVVDAKTGEVLALANMPTYNPNDRSELTGAQLRNRVVTDTFEPGSTLKPFTVALALDTH 330 + V+++ G + +L +N N + +T A +PGS+ KPF + +L+ Sbjct: 433 FVSVNSQDGAIRSLVGGFDFNRNKFNHVTQAWR---------QPGSSFKPFIYSSSLEKG 483 Query: 331 RVTPSTVFQTAPGKMTIGTATIGDSHAHGA-----LTVAQIIEKSSNIGTAKIALGMPAE 385 ++P+T+ AP T + +G +++ + ++KS N+ + +I + + Sbjct: 484 -LSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMRRALQKSINLVSIRILERVGVK 542 Query: 386 EMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWRPIEQATMSYGHGISVSLIQLAHAYLI 445 E T GF A + PY T++ G G +V+ +Q+ AY + Sbjct: 543 YAQEYITRFGFD-----------ADKNPPY--------LTLALGAG-NVTPLQMVGAYSV 582 Query: 446 FARNG-DIIPLSFTKVTDS--------------PIGQRVISADTALQMRRMLETVVAPGG 490 FA G I P TKVTDS RVI A A M ML VV G Sbjct: 583 FANGGYKINPYLITKVTDSNGEVLSQAKPATADDESNRVIDARNAFIMDSMLRDVVRHGT 642 Query: 491 TAPQAQVPGYRVGGKTGTAYKIENGKYVRKYVASFSGIAPMSDPRLIISVMIDEPQG--- 547 + + GKTGT + A F+G P I + D+P+ Sbjct: 643 AVKALSLKRTDLAGKTGTTNDSMD--------AWFAGYQP--GLTAIAWIGYDQPKSLGD 692 Query: 548 QHYGGPVAGPVFANVAANALR 568 + GG +A PV+ + + AL+ Sbjct: 693 RETGGGLALPVWISYMSKALK 713 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,741,991 Number of extensions: 70047 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 251 Number of HSP's successfully gapped: 3 Length of query: 592 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 501 Effective length of database: 1,188,985 Effective search space: 595681485 Effective search space used: 595681485 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)