BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0293.p (335 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 674 0.0 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 157 1e-39 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 144 1e-35 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 141 6e-35 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 105 5e-24 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 674 bits (1740), Expect = 0.0, Method: Compositional matrix adjust. Identities = 321/335 (95%), Positives = 329/335 (98%) Query: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIIASAREHGVNFIDTADVYTKGKSEE 60 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRI+ASAREHGVNFIDTADVYTKGKSEE Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVYTKGKSEE 60 Query: 61 IVGKLLAGQRHEWVLASKLGNAMSGKPNESHYSRSWIVRETENSLKRLDTDYLDILYMHR 120 IVGKLLAGQRHEWVLASKLGNAMSGKPNES+YSRSWIVRETEN L RLDTDYLDILYMHR Sbjct: 61 IVGKLLAGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTDYLDILYMHR 120 Query: 121 DYQDENLEEAVQALGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNL 180 DYQDENLEEAVQALGDLIR GKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNL Sbjct: 121 DYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNL 180 Query: 181 LNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPPEDTRAGRGDKRMMETEFR 240 LNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKP +DTRAGRGDKRMMETEFR Sbjct: 181 LNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDTRAGRGDKRMMETEFR 240 Query: 241 EESLLIAQRLQQHCEVRGIKLGQFATAWVLANAAISSVIAGPRTLAQMEDYYDAAELQIT 300 EESLLIAQRLQQHCE RG+KLGQFATAWVLAN+AISSVIAGPRTLAQMEDYYDAAEL+I+ Sbjct: 241 EESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKIS 300 Query: 301 AEEEALVDSLVRPGHASTHGYNDPNYPFFGRMVRG 335 AEEEALVDSLV PGHASTHGYNDPNYPFFGR+VRG Sbjct: 301 AEEEALVDSLVTPGHASTHGYNDPNYPFFGRVVRG 335 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 157 bits (396), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 114/317 (35%), Positives = 164/317 (51%), Gaps = 13/317 (4%) Query: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGR-IIASAREHGVNFIDTADVY--TKGK 57 M YRR G+S L + A+ LG D E+GR ++ A + GV D A+ Y G Sbjct: 14 MPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPPAGS 73 Query: 58 SEEIVGKLLAGQ----RHEWVLASKLGNAMSGKP-NESHYSRSWIVRETENSLKRLDTDY 112 +EE G+ +A R E V++SK G M P +R ++ E SLKR+ DY Sbjct: 74 AEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGARKHLIASCEQSLKRMGLDY 133 Query: 113 LDILYMHRDYQDENLEEAVQALGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPL 172 +DI Y HR D LEE + AL L R GK G+S++ + + + VPL L Sbjct: 134 VDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEGVPL-L 192 Query: 173 VCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPPEDTRAGRGDK 232 + QP Y++LNR E +LPA G+G + +SP+A+G+LT KY G PE R R Sbjct: 193 IHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGV--PEGARVTRAPS 250 Query: 233 RMMETEFREESLLIAQRLQQHCEVRGIKLGQFATAWVLANAAISSVIAGPRTLAQMEDYY 292 + + E++L + L + RG L Q A AWVL +A ++S + G RT+ Q++D Sbjct: 251 -LPQRMLSEDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGARTVEQLDDSL 309 Query: 293 DAA-ELQITAEEEALVD 308 DA +L T EE A +D Sbjct: 310 DAVNKLDFTQEELASID 326 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 144 bits (362), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 106/328 (32%), Positives = 161/328 (49%), Gaps = 18/328 (5%) Query: 4 RRLGKSNLKVSALCLGSMMFGD---QTDLQESGRIIASAREHGVNFIDTADVYTKGKSEE 60 RRLG + VSA+ LG M D Q D ES I A E G+N +DTAD+Y +EE Sbjct: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEE 77 Query: 61 IVGKLLAGQRHEWVLASKLGNAMSGKPNESHY-----SRSWIVRETENSLKRLDTDYLDI 115 ++G+ + G+R ++ +A+K G + P++ S ++I + E SLKRL + +D+ Sbjct: 78 LLGRAIKGRRDKFFIATKFG--IRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDL 135 Query: 116 LYMHRDYQDENLEEAVQALGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQ 175 Y HR +E + L DL++AGKIR G+S A L + V Q Sbjct: 136 YYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSE------ASAATLERAHRVHPVTALQ 189 Query: 176 PYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPPEDTRAGRGDKRMM 235 Y+L R PE E+L AC G+G V YSP+ RG LTG Q + ED R + R Sbjct: 190 SEYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFR-RSNPRFQ 248 Query: 236 ETEFREESLLIAQRLQQHCEVRGIKLGQFATAWVLANAAISSVIAGPRTLAQMEDYYDAA 295 F +L +A+++++ G Q A AWV+A I G + +ED + Sbjct: 249 GENF-ARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEDNAGSV 307 Query: 296 ELQITAEEEALVDSLVRPGHASTHGYND 323 +++ E+ +D++ G A+ Y D Sbjct: 308 GVKLAPEDLQALDAIFPRGAAAGERYTD 335 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 141 bits (356), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 101/349 (28%), Positives = 171/349 (48%), Gaps = 44/349 (12%) Query: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIIASAREHGVNFIDTADVY------- 53 M YR LG+++LKVS + LG+M +G+Q ++ + A E G+N ID A++Y Sbjct: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 Query: 54 TKGKSEEIVGKLL--AGQRHEWVLASKLGNAMSGKPNESH-------YSRSWIVRETENS 104 T+G +E +G L +G+R + ++A+K N H R + S Sbjct: 61 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEALHGS 120 Query: 105 LKRLDTDYLDILYMH--------------RDYQDEN---LEEAVQALGDLIRAGKIRGFG 147 L+RL D++D+ +H + +DE+ +EE + L + ++AGKIR G Sbjct: 121 LERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRTIG 180 Query: 148 VSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIA 207 +SN W +A+ +R E++ + + Q YNLLNR E+ + +G++ YSP+A Sbjct: 181 LSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLA 240 Query: 208 RGVLTGKYQPGQKPPEDTRAGRGDKRMMETEFREESLLIAQRLQQH----CEVRGIKLGQ 263 G+L+GKY G +P G + + F + A+R G+ Q Sbjct: 241 FGMLSGKYLDGARPA-------GGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQ 293 Query: 264 FATAWVLANAAISSVIAGPRTLAQMEDYYDAAELQITAEEEALVDSLVR 312 A A+V + ++S I G TLAQ+ D+AELQ+ + A ++++ R Sbjct: 294 MALAYVNSRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIEAIHR 342 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 105 bits (262), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 93/316 (29%), Positives = 149/316 (47%), Gaps = 22/316 (6%) Query: 1 MHYRRLGKSNLKVSALCLGSMMFGDQ---TDLQESGRIIASAREHGVNFIDTADVYTKGK 57 M RR G++ +VS + G+ G Q++ + +A + GV+FIDTADVY G+ Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 Query: 58 SEEIVGKLL---AGQRHEWVLASKLGNAMSGKPNESHYSRSWIVRETENSLKRLDTDYLD 114 SE+++ ++L G+R +A+K G +S + R+ + + SLK L D LD Sbjct: 61 SEQLIAQVLKERGGERP--FVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLD 118 Query: 115 ILYMHRDYQDENLEEAVQA-LGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLV 173 ++ +H + ++ V L L+ AGKIR +GVS ++ E ++ E NV Sbjct: 119 LVQLHCPPTEVYYQQEVFGHLDALVAAGKIRHYGVSV---EKVEEALKAIEYPNVK---S 172 Query: 174 CQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPPED-----TRAG 228 Q +N+ ++P L + V+ P+A G+LTGK D R G Sbjct: 173 VQIIFNIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHG 232 Query: 229 RG-DKRMMETEFREESLLIAQRLQQHCEVRGIKLGQFATAWVLANAAISSVIAGPRTLAQ 287 D + E L A + +G+ + QFA W+L + IS+VI G R +AQ Sbjct: 233 EAFDVGETFSGVPYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQ 292 Query: 288 MEDYYDAAEL-QITAE 302 + A+ L ITAE Sbjct: 293 AQSNSAASALPPITAE 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,090,359 Number of extensions: 45627 Number of successful extensions: 133 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 119 Number of HSP's successfully gapped: 6 Length of query: 335 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 249 Effective length of database: 1,212,660 Effective search space: 301952340 Effective search space used: 301952340 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)