BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0287.p (504 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 882 0.0 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 338 6e-94 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 279 3e-76 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 273 2e-74 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 251 1e-67 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 882 bits (2279), Expect = 0.0, Method: Compositional matrix adjust. Identities = 474/504 (94%), Positives = 485/504 (96%) Query: 1 MMTQSLTMTRLLRPVRIGSALLAALLVLAGCSVAPTYERPSVDTPAAFKEAAPTTQAGAN 60 MMTQ L+M+RLLRPVRIGSALLAALLVLAGCSVAPTYERPSVDTPAAFKEAAP TQ G N Sbjct: 1 MMTQPLSMSRLLRPVRIGSALLAALLVLAGCSVAPTYERPSVDTPAAFKEAAPATQTGEN 60 Query: 61 GSQWKTAQPAEDIHRGQWWKIFGDDKLNALEDTAQQANQDLKAAAARLGQARALTRDARS 120 GSQWKTAQPAEDI RG+WWKIFGD+KLNALEDTAQQANQDLKAAAARLGQARALTRDAR+ Sbjct: 61 GSQWKTAQPAEDIARGEWWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDARA 120 Query: 121 GYFPQVDTGVGATRQRTSPASQGLPADANTQPFTLYRAQVNVSYEVDLFGRVASTVDAAT 180 GYFPQVD GVGATRQR SPASQGLPADA+T+P TLYRAQVNVSYEVDLFGRVASTVDAAT Sbjct: 121 GYFPQVDAGVGATRQRPSPASQGLPADADTRPNTLYRAQVNVSYEVDLFGRVASTVDAAT 180 Query: 181 ADAQRSEALFHSVLLALQADVAQQYFQVRELDAAAELYRGTVDLRQESLRLVQRRFDEGD 240 ADAQRSEALFHSVLLALQADVAQQYF VRELDAAA LYRGTVDLRQESLRLVQRRFDEGD Sbjct: 181 ADAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGD 240 Query: 241 ISEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLNRLAVTVP 300 ISEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPL RLAVTVP Sbjct: 241 ISEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAVTVP 300 Query: 301 AGLPSTLLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSS 360 GLPS LLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSS Sbjct: 301 PGLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSS 360 Query: 361 RTFLLGPLVGTALSMPIFDGGRRQAGVDRARASYEEQVATYRGTVLNAFREVEDNLANLR 420 RTFLLGPLVGTALSMP+FDGGRRQAGVDRARASYEEQVA+YRGTVLNAFREVEDNLANLR Sbjct: 361 RTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLR 420 Query: 421 ILDEQNRAQDEAVAASTRAAHLSHTQYREGAVSYFDVIDADRTVLQQQRASVTLDGERAR 480 ILD+QNRAQDEAVAAS RAA LSHTQYREGAVSYF VIDADRTVLQQQRASVTLDGERAR Sbjct: 421 ILDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERAR 480 Query: 481 STVNLIRALGGGWGGPDTQLSSAK 504 STVNLIRALGGGWG P QLSSA+ Sbjct: 481 STVNLIRALGGGWGVPAEQLSSAR 504 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 338 bits (867), Expect = 6e-94, Method: Compositional matrix adjust. Identities = 212/492 (43%), Positives = 296/492 (60%), Gaps = 32/492 (6%) Query: 12 LRPVRIGSALLAALLVLAGCSVAPTYERPSVDT-PAAFKEAAPTTQAGANGSQWKTAQPA 70 L+P+ A+L A L LA CSV P Y RP +DT PA FKE G WK AQP Sbjct: 9 LKPLL---AMLVASLALAACSVGPDYVRPQMDTGPAIFKE----------GQNWKPAQPR 55 Query: 71 EDIHRGQWWKIFGDDKLNALEDTAQQANQDLKAAAARLGQARALTRDARSGYFPQVDTGV 130 + + G+WW++FGD +LNAL +NQ+L A A A AL + +RS Y P + Sbjct: 56 DLVADGKWWEMFGDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASA 115 Query: 131 GATRQRTSPASQGLPADA---------NTQPFTLYRAQVNVSYEVDLFGRVASTVDAATA 181 GA+R + A A N+ F+L ++E D++G+V V+++ A Sbjct: 116 GASRGSSGSAGASSSNAASGSSAGSVRNSYTFSL-----TAAWEADIWGKVRRDVESSQA 170 Query: 182 DAQRSEALFHSVLLALQADVAQQYFQVRELDAAAELYRGTVDLRQESLRLVQRRFDEGDI 241 AQ S A + L+ QA VAQ Y Q+R LDA +L TV + SL++ Q ++ G + Sbjct: 171 SAQASAADLAAARLSAQAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMV 230 Query: 242 SEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLNRLAVTVPA 301 ++ DV +A+ +L SAQ+ AL RA EHA+A+L+G+TPA+F+LP PL +A VP Sbjct: 231 AKSDVIQAQTQLKSAQASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVAPPVPQ 290 Query: 302 GLPSTLLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSR 361 G+PS LLERRPD+AAAER +A+ANA++GVAK+A+FPSL L+ GY+S+ D F R Sbjct: 291 GVPSMLLERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGR 350 Query: 362 TFLLGPLVGTALSMPIFDGGRRQAGVDRARASYEEQVATYRGTVLNAFREVEDNLANLRI 421 + LGP L+ IFDGG R A D+A A+Y+ VATY+ TVL AF+EVEDNLA LRI Sbjct: 351 VWSLGP----QLAQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRI 406 Query: 422 LDEQNRAQDEAVAASTRAAHLSHTQYREGAVSYFDVIDADRTVLQQQRASVTLDGERARS 481 L+++ Q EA+ A+ +A L + QY+ G VSY +VI A T L + +S+ + R + Sbjct: 407 LEQEAAVQGEALQAARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAA 466 Query: 482 TVNLIRALGGGW 493 +V L +ALGGGW Sbjct: 467 SVLLAKALGGGW 478 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 279 bits (714), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 193/468 (41%), Positives = 268/468 (57%), Gaps = 20/468 (4%) Query: 30 GCSVAPTYERPSVDTPAAFKEAAPTTQAGANGSQWKTAQPAEDIHRGQWWKIFGDDKLNA 89 GC+ P Y RP VD PAA+ AP W++ QPA+ RG WW+ FGD +LNA Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAP----------WRSMQPADATPRGPWWERFGDAQLNA 67 Query: 90 LEDTAQQANQDLKAAAARLGQARALTRDARSGYFPQVDTGVGATRQRTSPASQGLPADAN 149 L+ A NQ L A ARL QARA + PQV+ A R R S A++ L + N Sbjct: 68 LQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRIS-ANRPL-TNYN 125 Query: 150 TQPFTL----YRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQY 205 + F+ + +VSYEVD FGRV +V+AA A A++S A + L L A++A Y Sbjct: 126 SPNFSTVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNY 185 Query: 206 FQVRELDAAAELYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIAR 265 F +RELD ++ R + L++++L L R D G S +D+A+ +A L S ++ + R Sbjct: 186 FNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGR 245 Query: 266 QRATAEHALAILLGRTPAEFSLPPQPLNRLAVTVPAGLPSTLLERRPDIAAAERAMAAAN 325 QRA EHA+A L G FS+ P +P G+PS +LERRPDIAAAERAMAAAN Sbjct: 246 QRAQYEHAIATLTGTPAPAFSITPSLTPITPPAIPVGVPSDVLERRPDIAAAERAMAAAN 305 Query: 326 ARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPIFDGGRRQA 385 A++GVA +A++PS L G +S LFN S + L G + S +FDGGR +A Sbjct: 306 AQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSL----GVSASQSLFDGGRLRA 361 Query: 386 GVDRARASYEEQVATYRGTVLNAFREVEDNLANLRILDEQNRAQDEAVAASTRAAHLSHT 445 GVD ++A YE VA+YR TVL A +EVED + L L+ A+A++ R ++ + Sbjct: 362 GVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASS 421 Query: 446 QYREGAVSYFDVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 +Y GA Y DVI A +++L +R + L G+R +V LI+ALGG W Sbjct: 422 RYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDW 469 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 273 bits (699), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 197/497 (39%), Positives = 280/497 (56%), Gaps = 38/497 (7%) Query: 14 PVRIGSALLAALL--VLAGCSVAPTYERPSVDTPAAFKEAAPTTQAGANGSQWKTAQPAE 71 P R + A +L +L GC+V P Y RP++D P A+KE P WK A P + Sbjct: 28 PFRATKGMTALVLAALLGGCAVGPDYARPALDLPQAYKETGP----------WKLAAPRQ 77 Query: 72 DIHRGQWWKIFGDDKLNALEDTAQQANQDLKAAAARLGQARALTRDARSGYFPQVDTGVG 131 WW+ +GD LNAL D A ANQ ++ AAA+ QA+A AR+ +P + G Sbjct: 78 IDDHQPWWEAYGDPVLNALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAG 137 Query: 132 ATRQRTSPASQGLPADANTQPFTLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFH 191 A+R +T+ + G+ N+ Y +N S+E DL+GR+ +A A+AQ S A Sbjct: 138 ASRAQTN--TNGVQRLGNS-----YSVGLNASWEADLWGRIRRGAEAGEANAQASAASLA 190 Query: 192 SVLLALQADVAQQYFQVRELDAAAELYRGTVDLRQESLRLVQRRFDEGDISEVDVARARA 251 + L++QA +AQ Y Q+R D +LYR TV SL+L +++ G DVA+A Sbjct: 191 AARLSIQATLAQDYLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAET 250 Query: 252 ELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQP---------------LNRLA 296 +L +AQ++ + + R EHA+A+L+G+ PA+FSLP P L Sbjct: 251 QLRAAQAQLIDLDDTRNQLEHAIAMLIGKAPAQFSLPALPPAAASDSAIDANAARLQGSL 310 Query: 297 VTVPAGLPSTLLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLF 356 +P GLPS LLERRPDIA AER AAANA +GVA++A+FP+L L+ GY S +LF Sbjct: 311 PQIPVGLPSELLERRPDIANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANLF 370 Query: 357 NWSSRTFLLGPLVGTALSMPIFDGGRRQAGVDRARASYEEQVATYRGTVLNAFREVEDNL 416 N SR + LG +AL+ +FDGG R A D A A+Y+ VA Y+ TVL +EVED L Sbjct: 371 NTPSRVWSLG----SALAESLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKL 426 Query: 417 ANLRILDEQNRAQDEAVAASTRAAHLSHTQYREGAVSYFDVIDADRTVLQQQRASVTLDG 476 + LR+LD+++ Q +AV ++ A L+ QY G V+Y V+ L QR +VTL G Sbjct: 427 STLRVLDQESTVQAQAVQSAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVTLLG 486 Query: 477 ERARSTVNLIRALGGGW 493 + ++V LI+A GGGW Sbjct: 487 RQLVASVALIKATGGGW 503 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 251 bits (641), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 181/494 (36%), Positives = 260/494 (52%), Gaps = 31/494 (6%) Query: 5 SLTMTRLLRPVRIGSALLAALLVLAGCSVAPTYERPSVDTPAAFKEAAPTTQAGANGSQW 64 +L+M R R + + +AL+ A VL GCS+APTYERP +++ P AG Q Sbjct: 3 NLSMPRSPRLLHMCAALVMAG-VLGGCSLAPTYERPQAPVESSY----PADAAGRAELQA 57 Query: 65 KTAQPAEDIHRGQWWKIFGDDKLNALEDTAQQANQDLKAAAARLGQARALTRDARSGYFP 124 ++ G W + F D +L L A + N+DL+ A + +ARA + AR+ P Sbjct: 58 --------VNLG-WRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLP 108 Query: 125 QVDTGVGATRQRTSPASQGLPADANTQPFTLYRAQVNVS---YEVDLFGRVASTVDAATA 181 ++ A R RT+ ASQ L QP QV ++ YE+D FGRV + DAA A Sbjct: 109 NLNLSGNAARTRTA-ASQSLTG----QPTISNSYQVGLAVPAYELDFFGRVRNLKDAALA 163 Query: 182 DAQRSEALFHSVLLALQADVAQQYFQVRELDAAAELYRGTVDLRQESLRLVQRRFDEGDI 241 +E S ++L A VAQ Y R +L T+ RQ +L L Q+R D G Sbjct: 164 SYLSTEEAQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGAS 223 Query: 242 SEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPL---NRLAVT 298 + +D + + SA+ + RQRA AE+AL +L+G+ E LPP L ++ Sbjct: 224 TALDFRLTQTLVESARVAKTQLERQRAQAENALTLLVGKKIGE--LPPAQLLSDEKIVTD 281 Query: 299 VPAGLPSTLLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNW 358 +PAGLPS LL RPDI +AE+++ AANA +G A++AFFP ++LTG G S+ LG LF Sbjct: 282 IPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFES 341 Query: 359 SSRTFLLGPLVGTALSMPIFDGGRRQAGVDRARASYEEQVATYRGTVLNAFREVEDNLAN 418 S + GP +LS+PIFD GR A +D A + V TY + AFREV D L Sbjct: 342 GSGAWSFGP----SLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVA 397 Query: 419 LRILDEQNRAQDEAVAASTRAAHLSHTQYREGAVSYFDVIDADRTVLQQQRASVTLDGER 478 LDEQ +AQ+ +AA L+ +++ G S D +DA R + Q+A + R Sbjct: 398 RGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLR 457 Query: 479 ARSTVNLIRALGGG 492 + ++L +ALGGG Sbjct: 458 LNNAIDLYKALGGG 471 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.129 0.363 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,371,359 Number of extensions: 52486 Number of successful extensions: 304 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 19 Length of query: 504 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 415 Effective length of database: 1,198,455 Effective search space: 497358825 Effective search space used: 497358825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)