BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0276.p (280 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 465 e-132 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 102 4e-23 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 86 4e-18 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 86 4e-18 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 80 2e-16 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 465 bits (1197), Expect = e-132, Method: Compositional matrix adjust. Identities = 224/280 (80%), Positives = 248/280 (88%) Query: 1 MKQIPLITLRNGAQVPTLGQGTWNMGESGAAAATEVRALQAGIDLGMTLIDTAEMYADGN 60 M+ IPL++LRNG VP LGQGTWNMGES A A EVRALQAG+DLGM+LIDTAEMYA+G Sbjct: 1 MQSIPLLSLRNGQSVPILGQGTWNMGESPAQANAEVRALQAGLDLGMSLIDTAEMYAEGG 60 Query: 61 AERIVGQAIAGRRQQVYLVSKVLPHNASQRGTIAACEASLKRLGTDHIELYLLHWRGPHP 120 AERIV +AIAGRR QVYLVSKVLPHNAS+RGTIAACEASLKRLGTD I+LYLLHWRG HP Sbjct: 61 AERIVAKAIAGRRDQVYLVSKVLPHNASKRGTIAACEASLKRLGTDRIDLYLLHWRGAHP 120 Query: 121 LAATVEGMEKLVAQGKIGGWGVSNLDSDDIDELLQEPQGSHYLCNQVLYNLSRRGIEYDL 180 LAAT+EGME LV+QGKIGGWGVSN D+DDID+LL+EP G HYLCNQVLYNLSRRGIEYDL Sbjct: 121 LAATIEGMETLVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGIEYDL 180 Query: 181 LPQALEQGVAIMAYSPIEQGRILKNATLGTVAQRHGVTPAQVALAWVLRRPGVIAIPKAA 240 LPQA + +A+MAYSPIEQGRILK+ L +AQ H VTPAQ+ALAWVLR+PGV+AIPKAA Sbjct: 181 LPQAQQAHIAVMAYSPIEQGRILKHPALTALAQAHRVTPAQIALAWVLRQPGVVAIPKAA 240 Query: 241 DTAHVQANRACLDLQLTAADLKELDAAFPPPRRKQPLAML 280 D AHVQANRA LD+ L+AADLK LD AF PPRRKQPLAML Sbjct: 241 DVAHVQANRASLDISLSAADLKILDEAFAPPRRKQPLAML 280 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 102 bits (254), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 88/306 (28%), Positives = 137/306 (44%), Gaps = 51/306 (16%) Query: 11 NGAQVPTLGQGTWNMGESGAAA--ATEVRALQAGIDLGMTLIDTAEMYADGNAERIVGQA 68 NG V +G G M + A A + + ++LG+ L+DTA+MY E ++G+A Sbjct: 23 NGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEELLGRA 82 Query: 69 IAGRRQQVYLVSKV----LPHNASQRGTIA-------ACEASLKRLGTDHIELYLLHWRG 117 I GRR + ++ +K P + RG A E SLKRLG + I+LY H Sbjct: 83 IKGRRDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDLYYQHRID 142 Query: 118 P-HPLAATVEGMEKLVAQGKIGGWGVSNLDSDDIDELLQEPQGSHYLCN-QVLYNLSRRG 175 P P+ T+ + LV GKI G+S + L+ H + Q Y+L R Sbjct: 143 PATPIEVTMGVLADLVQAGKIRYIGLSEASA----ATLERAHRVHPVTALQSEYSLWTRD 198 Query: 176 IEYDLLPQALEQGVAIMAYSPIEQG---------------------------RILKNATL 208 E ++L G+ +AYSP+ +G +N L Sbjct: 199 PEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFRRSNPRFQGENFARNLQL 258 Query: 209 G----TVAQRHGVTPAQVALAWVL-RRPGVIAIPKAADTAHVQANRACLDLQLTAADLKE 263 +A +HG TP+Q+ALAWV+ + P ++ IP +++ N + ++L DL+ Sbjct: 259 AEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEDNAGSVGVKLAPEDLQA 318 Query: 264 LDAAFP 269 LDA FP Sbjct: 319 LDAIFP 324 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 85.9 bits (211), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 92/336 (27%), Positives = 140/336 (41%), Gaps = 83/336 (24%) Query: 17 TLGQGTWNMGESGAAAATEVRALQAGIDLGMTLIDTAEMY-------ADGNAERIVGQAI 69 TLG TW S A A + L ++ G+ LID AEMY G ER +G + Sbjct: 17 TLGTMTWGEQNSEADAHAQ---LDYAVERGINLIDVAEMYPVPPKAETQGLTERYLGTWL 73 Query: 70 --AGRRQQVYLVSKVL-----PHNA----------SQRGTIAACEASLKRLGTDHIELYL 112 +G+R Q+ + +K PHN ++G A SL+RL DH++LY Sbjct: 74 KKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEALHGSLERLQLDHVDLYQ 133 Query: 113 LHW------------------RGPHPLAATVEGMEKLVAQGKIGGWGVSNLDSDDIDELL 154 LHW P+ T+ + + V GKI G+SN + + + L Sbjct: 134 LHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRTIGLSNETAWGVAQFL 193 Query: 155 Q--EPQGSHYLCN-QVLYNLSRRGIEYDLLPQALEQGVAIMAYSPIEQGRI--------- 202 + E G + Q YNL R E +L A + V ++AYSP+ G + Sbjct: 194 RAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLAFGMLSGKYLDGAR 253 Query: 203 ---------------------LKNATLGTVAQRHGVTPAQVALAWVLRRPGVIA-IPKAA 240 A +A++HG+ PAQ+ALA+V RP + + I A Sbjct: 254 PAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMALAYVNSRPFLTSNIIGAT 313 Query: 241 DTAHVQANRACLDLQLTAADLKELDAAFPPPRRKQP 276 A +++N +LQL +++ AA R+QP Sbjct: 314 TLAQLRSNIDSAELQLG----EDVLAAIEAIHRRQP 345 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 85.5 bits (210), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 89/314 (28%), Positives = 144/314 (45%), Gaps = 59/314 (18%) Query: 10 RNGAQVPTLGQGTWNMGES-GAAAATEVRA-LQAGIDLGMTLIDTAEMYADGNAERIVGQ 67 R GAQV +G G W +G S G +A + +A L A +D G++ IDTA++Y DG +E+++ Q Sbjct: 8 RTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGRSEQLIAQ 67 Query: 68 AIAGRRQQVYLVS-----KVLPHNAS---QRGTIAA-CEASLKRLGTDHIELYLLHWRGP 118 + R + V+ ++ PH AS R ++A + SLK LG D ++L LH P Sbjct: 68 VLKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLDLVQLHC-PP 126 Query: 119 HPLAATVE---GMEKLVAQGKIGGWGVSNLDSDDIDELLQEPQGSHYLCNQVLYNLSRRG 175 + E ++ LVA GKI +GVS + ++E L+ + + Q+++N+ R+ Sbjct: 127 TEVYYQQEVFGHLDALVAAGKIRHYGVS---VEKVEEALKAIEYPNVKSVQIIFNIFRQR 183 Query: 176 IEYDLLPQALEQGVAIMAYSPIEQGRILKNATLGTV--------AQRH------------ 215 L +A + +A++A P+ G + + T RH Sbjct: 184 PISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSG 243 Query: 216 -------------------GVTPAQVALAWVLRRPGV-IAIPKAADTAHVQANRACLDL- 254 GV AQ AL W+L G+ IP A + A Q+N A L Sbjct: 244 VPYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAASALP 303 Query: 255 QLTAADLKELDAAF 268 +TA ++ L A + Sbjct: 304 PITAEVMQALRAIY 317 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 80.1 bits (196), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 56/176 (31%), Positives = 92/176 (52%), Gaps = 12/176 (6%) Query: 37 RALQAGIDLGMTLIDTAEMYADGNAERIVGQAIAGRRQQVYLVSKV------LPH--NAS 88 R + + + G+ IDTA++Y G +E IVG+ +AG+R + L SK+ P+ N S Sbjct: 34 RIVASAREHGVNFIDTADVYTKGKSEEIVGKLLAGQRHEWVLASKLGNAMSGKPNESNYS 93 Query: 89 QRGTIAACEASLKRLGTDHIELYLLHW-RGPHPLAATVEGMEKLVAQGKIGGWGVSNLDS 147 + + E L RL TD++++ +H L V+ + L+ GKI G+GVSN Sbjct: 94 RSWIVRETENILTRLDTDYLDILYMHRDYQDENLEEAVQALGDLIRVGKIRGFGVSNFRG 153 Query: 148 DDIDELLQEPQGSHY---LCNQVLYNLSRRGIEYDLLPQALEQGVAIMAYSPIEQG 200 I E+++ + ++ L Q YNL R E ++LP G+ ++ YSPI +G Sbjct: 154 WRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARG 209 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 923,494 Number of extensions: 39415 Number of successful extensions: 167 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 6 Length of query: 280 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 196 Effective length of database: 1,222,130 Effective search space: 239537480 Effective search space used: 239537480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)