BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0275.p (418 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309641|ref|YP_003773733.1| heat-shock Hsp70 protein [Herba... 736 0.0 gi|300312439|ref|YP_003776531.1| molecular chaperone protein [He... 64 2e-11 gi|300312141|ref|YP_003776233.1| molecular chaperone, Hsp70 fami... 64 2e-11 >gi|300309641|ref|YP_003773733.1| heat-shock Hsp70 protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 736 bits (1900), Expect = 0.0, Method: Compositional matrix adjust. Identities = 387/417 (92%), Positives = 398/417 (95%) Query: 1 MANACGVDFGTSNSTVGWQRPQAGDSLLLPLEDGRATLPSVVFFNADENSFSYGRAGLGE 60 MANACGVDFGTSNSTVGW RP A DSLLLPLEDG+ATLPSVVFFNADEN FSYGRAGLG+ Sbjct: 1 MANACGVDFGTSNSTVGWLRPDAPDSLLLPLEDGKATLPSVVFFNADENQFSYGRAGLGD 60 Query: 61 YLAGYEGRLMRSLKSLLGSGAIDGQTEVGGRALPYRGLLSQFIGELKQRAERAAGTAFSQ 120 YLAGYEGRLMRSLKSLLGSGAIDGQTEVGGRALPYRGLLSQFIGELK RA+RAAGT+FS Sbjct: 61 YLAGYEGRLMRSLKSLLGSGAIDGQTEVGGRALPYRGLLSQFIGELKMRAQRAAGTSFSS 120 Query: 121 VVLGRPVHFVDDDAAADQLAQDTLEQIARDVGFKDIAFQFEPIAAAFDYESRIDREELVL 180 VVLGRPVHFVDDD ADQLAQDTLEQIARDVGFKDIAFQFEPIAAAFDYESRIDREELVL Sbjct: 121 VVLGRPVHFVDDDDQADQLAQDTLEQIARDVGFKDIAFQFEPIAAAFDYESRIDREELVL 180 Query: 181 IVDIGGGTSDFSLVRLSPQRASRSERRDDILANGGVHIGGTDFDKYFSLACVMPLLGLGG 240 IVDIGGGTSDFSLVRLSPQRA R+ERRDDILANGGVHIGGTDFDKYFSL+CVMPLLGLGG Sbjct: 181 IVDIGGGTSDFSLVRLSPQRAQRAERRDDILANGGVHIGGTDFDKYFSLSCVMPLLGLGG 240 Query: 241 RLLGNAEIPSSYYFNLATWHTINQLYTRKAWQQLQDVAREVADRERFARLLDVVEQRCGH 300 RL GNAEIPSSYYFNLATWHTINQLYTRK WQQLQDV REVADRERFARLLDVVEQRCGH Sbjct: 241 RLQGNAEIPSSYYFNLATWHTINQLYTRKVWQQLQDVGREVADRERFARLLDVVEQRCGH 300 Query: 301 WLALQVEAGKIALSSADQTLLDLERLRKPEQLQLQRSDFDAAVDHLVQSVEQTVLALLQQ 360 WLALQVEAGKIALSSADQTLLDLERLR PEQL LQR DFD AV+HLVQSVEQTVL+LL++ Sbjct: 301 WLALQVEAGKIALSSADQTLLDLERLRNPEQLSLQRRDFDLAVEHLVQSVEQTVLSLLKE 360 Query: 361 AGLRADDIDSVFFTGGSSGVRLLRERIGALAPKARHVEGDLFGSIGSGLAIDAVRKF 417 AGL DDIDSVFFTGGSSGVRLLRERIGALAP+ARHVEGDLFGSIGSGLAIDA RKF Sbjct: 361 AGLGPDDIDSVFFTGGSSGVRLLRERIGALAPQARHVEGDLFGSIGSGLAIDAARKF 417 >gi|300312439|ref|YP_003776531.1| molecular chaperone protein [Herbaspirillum seropedicae SmR1] Length = 621 Score = 64.3 bits (155), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 106/410 (25%), Positives = 156/410 (38%), Gaps = 103/410 (25%) Query: 4 ACGVDFGTSNSTVGWQRPQAGDSLLLPLEDGRATLPSVVFFNADENSFSYGRAGLGEYLA 63 A G+D GT+NS V R + +L +DGR LPSVV + G A +G Sbjct: 21 AVGIDLGTTNSLVATVRNSIPE--VLADDDGRVLLPSVVRY------LPNGHANIGYKAQ 72 Query: 64 GYEGRLMR----SLKSLLGSGAID---------------GQTE---VGGRALPYRGLLSQ 101 + R S+K +G G D G + V G P + +Q Sbjct: 73 AAQTTDPRNTIVSVKRFMGRGLKDIAHAENMPYDFQDQPGMVQIKTVAGVKSPVE-VSAQ 131 Query: 102 FIGELKQRAERAAGTAFSQVVLGRPVHFVDDDAAADQLAQDTLEQIARDVGFKDIAFQFE 161 + L+Q AE A G V+ P +F D A Q +D A+ G + E Sbjct: 132 ILATLRQTAEDALGDDLVGAVITVPAYF---DDAQRQATKDA----AQLAGLNVLRLLNE 184 Query: 162 PIAAAFDYESRIDREELVLIVDIGGGTSDFSLVRLSPQRASRSERRDDILANGG-VHIGG 220 P AAA Y E + + D+GGGT D S+++L + ++LA GG +GG Sbjct: 185 PTAAAIAYGLDNSSEGVFAVYDLGGGTFDISILKL-------TRGVFEVLATGGDSALGG 237 Query: 221 TDFDKYFSLACVMPLLGLGGRLLGNAEIPSSYYFNLATWHTINQLYTRKAWQQLQDVARE 280 DFD L W + +A QLQ ++ E Sbjct: 238 DDFD-----------------------------HRLFCW------ISHEA--QLQPLSDE 260 Query: 281 VADRERFARLLDVVEQRCGHWLALQVEAGKIALSSADQTLLDLERLRKPEQLQLQ--RSD 338 RLL V + L S+ D+ +D LR E++ L + Sbjct: 261 ------DTRLLMVKAREVKELL-----------STKDEVQID-AVLRSGEEVHLSITAAQ 302 Query: 339 FDAAVDHLVQSVEQTVLALLQQAGLRADDIDSVFFTGGSSGVRLLRERIG 388 F+ HLV V L+ AGL +D+D V GG++ + +R +G Sbjct: 303 FNEITQHLVAKTMAPVRKALRDAGLTVEDVDGVVLVGGATRMPHIRRAVG 352 >gi|300312141|ref|YP_003776233.1| molecular chaperone, Hsp70 family [Herbaspirillum seropedicae SmR1] Length = 642 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 79/253 (31%), Positives = 107/253 (42%), Gaps = 48/253 (18%) Query: 4 ACGVDFGTSNSTVGWQRPQAGDSLLLPLEDGRATLPSVVFF-------NADENSFSYGRA 56 A G+D GT+NS V Q+G + +LP E G+A LPSVV + AD G A Sbjct: 21 AVGIDLGTTNSLVATV--QSGQARILPDEAGQALLPSVVRYLPAHSLQPADRPPAVVGHA 78 Query: 57 GLGEYLAGYEGRLMRSLKSLLGSGAIDGQTEVGGRA-LPY-----RGLL----------- 99 L S+K L+G D G A +PY GL+ Sbjct: 79 AKAAQSDDPRNTL-SSIKRLMGRDRAD--ISAGNSADMPYAFIDKPGLVQIDTAAGIKTP 135 Query: 100 ----SQFIGELKQRAERAAGTAFSQVVLGRPVHFVDDDAAADQLAQDTLEQIARDVGFKD 155 + + L+QRAE + G S V+ P +F D A Q +D AR G Sbjct: 136 IDVSADILRVLRQRAEASLGGELSGAVITVPAYF---DDAQRQATKDA----ARLAGLNV 188 Query: 156 IAFQFEPIAAAFDYESRIDREELVLIVDIGGGTSDFSLVRLSPQRASRSERRDDILA-NG 214 + EP AAA Y E + I D+GGGT D S+++L S ++LA NG Sbjct: 189 LRLLNEPTAAAIAYGLDQAAEGVFAIYDLGGGTFDISILKL-------SRGVFEVLATNG 241 Query: 215 GVHIGGTDFDKYF 227 +GG DFD+ Sbjct: 242 DGALGGDDFDRCI 254 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,281,144 Number of extensions: 54265 Number of successful extensions: 194 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 194 Number of HSP's successfully gapped: 3 Length of query: 418 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 330 Effective length of database: 1,203,190 Effective search space: 397052700 Effective search space used: 397052700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)