BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0254.p (490 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309611|ref|YP_003773703.1| outer membrane channel lipoprot... 684 0.0 gi|300311703|ref|YP_003775795.1| ABC-type export system outer me... 229 4e-61 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 137 2e-33 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 134 2e-32 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 130 2e-31 >gi|300309611|ref|YP_003773703.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 493 Score = 684 bits (1765), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/460 (79%), Positives = 413/460 (89%) Query: 22 SGCALGPSGEAPAMPQPAHYAVESTPATLGPQEARQKFVLGQRAVPQWWQAYGSPQLSAW 81 SGCA GP+GEAPAMP+P HYA ++TPA +GP+ ARQ+FVLGQRAVPQWWQAYGSP+LSAW Sbjct: 19 SGCAFGPTGEAPAMPRPDHYAAQATPADVGPEGARQRFVLGQRAVPQWWQAYGSPELSAW 78 Query: 82 VDEALRNNHSLAQAERNLAGAQLQLRAQVGESLWPTIDAVGQTQRQRALGLPNIGPSTNV 141 V+EALRNNHSLAQAERNLA A+LQLRAQ+ +SLWP++DAVGQ QRQRALGLPNIGP+TN+ Sbjct: 79 VEEALRNNHSLAQAERNLASARLQLRAQIDQSLWPSVDAVGQAQRQRALGLPNIGPTTNL 138 Query: 142 YNVFSGQIRASYTFDLFGVQRFANSALAAQVDAQSYQFDAARRTVAANVVISAINAASLR 201 YNVF+GQIRASYTFDLFG +R+AN+A AAQVDAQSYQFDAARR + ANVVISAINAA+LR Sbjct: 139 YNVFAGQIRASYTFDLFGTERYANAATAAQVDAQSYQFDAARRALVANVVISAINAAALR 198 Query: 202 AQVAQTERLVALADADVEEMQRRLALGAVSTDELLSARASAQSLRASLPGLKAQWQGARH 261 QV+ TERLVALA+AD E+QRR LGA S D+LL+ARA+AQSLRASLPGL+AQW ARH Sbjct: 199 VQVSDTERLVALAEADASEVQRRAELGAASQDDLLAARANAQSLRASLPGLQAQWLAARH 258 Query: 262 ALAVLMGRTPDAAPPDLDIAALTLPAEVPMTVPSDLLRQRPDILAADAALKVAAAQQAEA 321 ALAVLMGRTPD AP DLD+A+L LPAEVP+ VPS+LLRQRPDILAADAALKVAAAQQA+A Sbjct: 259 ALAVLMGRTPDQAPADLDLASLRLPAEVPLAVPSELLRQRPDILAADAALKVAAAQQAQA 318 Query: 322 TAEMFPSLSINASFGQSGFSWAKATSGVGAVWGLGASLTQPLFHGGALRAKRDAAKEAYL 381 TAEMFPSLSI+ASFGQSGF+W KAT GAVWGLGASLTQPLFHGGALR+ +AA++AYL Sbjct: 319 TAEMFPSLSISASFGQSGFTWPKATGAAGAVWGLGASLTQPLFHGGALRSMSEAAEQAYL 378 Query: 382 AAQSHYRQTVLNAFQNVADSLVALTQDADALQASAAARTASEQSWRNAQRRAQLGAYPAS 441 AA+++YRQTVLNAFQNVADSLVAL+ DADA A+AAAR ASEQ WRNAQRR LGA P S Sbjct: 379 AAEANYRQTVLNAFQNVADSLVALSHDADAQTAAAAARQASEQGWRNAQRRVALGAVPIS 438 Query: 442 ATRAGERQYLNARLGEIRATASRMSDTATLFQAMGVGLEA 481 A R+GERQYLNA L E RATASR++ TATLFQAMGV LEA Sbjct: 439 AARSGERQYLNAHLSEARATASRLNATATLFQAMGVSLEA 478 >gi|300311703|ref|YP_003775795.1| ABC-type export system outer membrane channel protein [Herbaspirillum seropedicae SmR1] Length = 489 Score = 229 bits (584), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 170/465 (36%), Positives = 253/465 (54%), Gaps = 14/465 (3%) Query: 22 SGCALGPSGEAPAMPQPAHYAVESTPATL---GPQEARQKFVLGQRAVPQWWQAYGSPQL 78 +GC +GP AP P+ + Y + P L + Q+ ++G+ +WWQ +GSP+L Sbjct: 22 AGCLVGPDFVAPTPPRVSQYTRD--PVLLDRMSEPDMSQQLLMGENPPARWWQLFGSPEL 79 Query: 79 SAWVDEALRNNHSLAQAERNLAGAQLQLRAQVGESLWPTIDAVGQTQRQRALGLPNIGPS 138 A V +AL N +L A NL A+ LRA G +P +DA +R R+ L G S Sbjct: 80 DAAVTQALTRNPTLQAAVSNLLLAEDNLRAG-GAVFYPRLDANMMAERTRSASLVQ-GSS 137 Query: 139 --TNVYNVFSGQIRASYTFDLFGVQRFANSALAAQVDAQSYQFDAARRTVAANVVISAIN 196 V++V + SY DLFG +R L A VD Q AA T+ ANVV + I Sbjct: 138 APDKVFSVATLSGAVSYAIDLFGEKRRTLEQLEAMVDVQQQLHHAAYLTLTANVVNAYIA 197 Query: 197 AASLRAQVAQTERLVALADADVEEMQRRLALGAVSTDELLSARASAQSLRASLPGLKAQW 256 A +Q++ +++AL + +E +Q R+ G +LLS + +A L L + Sbjct: 198 RAGYLSQISLVRQIIALEELQLEAIQARVDTGVAPLADLLSQASLLAGNQALLAQLTQRL 257 Query: 257 QGARHALAVLMGRTP-DAAPPDLDIAALTLPAEVPMTVPSDLLRQRPDILAADAALKVAA 315 H LA L G P A+ P+L +A L LP+++P+++PS L+R+RPDILAA+A + A+ Sbjct: 258 YQIEHLLAQLQGEFPAQASLPELVLADLHLPSDIPVSLPSALVRRRPDILAAEARMHAAS 317 Query: 316 AQQAEATAEMFPSLSINASFGQSGFSWAKATSGVGAVWGLGASLTQPLFHGGALRAKRDA 375 A ++A +PSL + A++G + + ++ T ++W G SL+ PLF+GG LRA+RDA Sbjct: 318 AAIGMSSAAQYPSLELTATYGVAARNLSQLTGPATSLWSFGPSLSTPLFNGGRLRAQRDA 377 Query: 376 AKEAYLAAQSHYRQTVLNAFQNVADSLVALTQDADALQA-SAAARTASEQ-SWRNAQRRA 433 A A+ A+QS Y+QTVL AF+ VAD+L AL D + A SAAAR A + + A A Sbjct: 378 AVNAFEASQSEYQQTVLTAFEQVADALRALQADGHIVSAQSAAARYAYDTLLLQRASYSA 437 Query: 434 QLGAYPASATRAGERQYLNARLGEIRATASRMSDTATLFQAMGVG 478 L +Y + E QY +A + ++AT R DT L A G G Sbjct: 438 GLSSY--ADMLLYEVQYRSAAIASLQATVQRYQDTVALLVASGGG 480 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 137 bits (345), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 154/485 (31%), Positives = 228/485 (47%), Gaps = 37/485 (7%) Query: 23 GCALGPSGEAPAMPQPAHYAVESTPATLGPQEARQKFVLGQRAVP-------QWWQAYGS 75 GC++ P+ E P++ PA + E+ PAT + Q + A P +WW+ +G Sbjct: 30 GCSVAPTYERPSVDTPAAFK-EAAPATQTGENGSQ----WKTAQPAEDIARGEWWKIFGD 84 Query: 76 PQLSAWVDEALRNNHSLAQAERNLAGAQLQLR-AQVGESLWPTIDA-VGQTQRQR----A 129 +L+A D A + N L A L A+ R A+ G +P +DA VG T RQR + Sbjct: 85 EKLNALEDTAQQANQDLKAAAARLGQARALTRDARAG--YFPQVDAGVGAT-RQRPSPAS 141 Query: 130 LGLPNIGPSTNVYNVFSGQIRASYTFDLFGVQRFANSALAAQVDAQSYQ--FDAARRTVA 187 GLP T ++ Q+ SY DLFG R A++ AA DAQ + F + + Sbjct: 142 QGLP-ADADTRPNTLYRAQVNVSYEVDLFG--RVASTVDAATADAQRSEALFHSVLLALQ 198 Query: 188 ANVVISAINAASLRAQVAQTERLVALADADVEEMQRRLALGAVSTDELLSARASAQSLRA 247 A+V L A A V L + +QRR G +S ++ ARA S ++ Sbjct: 199 ADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQS 258 Query: 248 SLPGLKAQWQGARHALAVLMGRTPDAAPPDLDIAALT-LPAEVPMTVPSDLLRQRPDILA 306 G+ Q A HALA+L+GRTP A L LT L VP +PS LL +RPDI A Sbjct: 259 EALGIARQRATAEHALAILLGRTP--AEFSLPPQPLTRLAVTVPPGLPSALLERRPDIAA 316 Query: 307 ADAALKVAAAQQAEATAEMFPSLSINASFGQSG------FSWAKATSGVGAVWGLGASLT 360 A+ A+ A A+ A + FPSL++ G F+W+ T +G + +G +L+ Sbjct: 317 AERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPL--VGTALS 374 Query: 361 QPLFHGGALRAKRDAAKEAYLAAQSHYRQTVLNAFQNVADSLVALTQDADALQASAAART 420 PLF GG +A D A+ +Y + YR TVLNAF+ V D+L L +A A Sbjct: 375 MPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVA 434 Query: 421 ASEQSWRNAQRRAQLGAYPASATRAGERQYLNARLGEIRATASRMSDTATLFQAMGVGLE 480 AS ++ + + + + GA + +R L + + R T L +A+G G Sbjct: 435 ASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGWG 494 Query: 481 AQAQQ 485 A+Q Sbjct: 495 VPAEQ 499 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 134 bits (337), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 133/462 (28%), Positives = 202/462 (43%), Gaps = 21/462 (4%) Query: 23 GCALGPSGEAPAMPQPAHYAVESTPATLGPQEARQKFVLGQRAVPQWWQAYGSPQLSAWV 82 GCA GP P + PA + E+ ++ P +A + WW+ +G QL+A Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQPADATPR--------GPWWERFGDAQLNALQ 69 Query: 83 DEALRNNHSLAQAERNLAGAQLQLRAQVGESLWPTIDAVGQTQRQRA--------LGLPN 134 +AL N +LA A LA A+ QL G + P ++ + R R PN Sbjct: 70 QQALAGNQTLAIATARLAQARAQLNV-TGAAQTPQVNLNARAARSRISANRPLTNYNSPN 128 Query: 135 IGPSTNVYNVFSGQIRASYTFDLFGVQRFANSALAAQVDAQSYQFDAARRTVAANVVISA 194 + V N F+ SY D FG + + A A + + + R + A + + Sbjct: 129 F---STVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNY 185 Query: 195 INAASLRAQVAQTERLVALADADVEEMQRRLALGAVSTDELLSARASAQSLRASLPGLKA 254 N L ++ R +AL +E R LGA S ++ +A S + L Sbjct: 186 FNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGR 245 Query: 255 QWQGARHALAVLMGRTPDAAPPDLDIAALTLPAEVPMTVPSDLLRQRPDILAADAALKVA 314 Q HA+A L G A + +T PA +P+ VPSD+L +RPDI AA+ A+ A Sbjct: 246 QRAQYEHAIATLTGTPAPAFSITPSLTPITPPA-IPVGVPSDVLERRPDIAAAERAMAAA 304 Query: 315 AAQQAEATAEMFPSLSINASFGQSGFSWAKATSGVGAVWGLGASLTQPLFHGGALRAKRD 374 AQ A+A +PS + S+G +WA + +W LG S +Q LF GG LRA D Sbjct: 305 NAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRAGVD 364 Query: 375 AAKEAYLAAQSHYRQTVLNAFQNVADSLVALTQDADALQASAAARTASEQSWRNAQRRAQ 434 ++ Y A + YR+TVL A Q V D + L A S A ++ + A R + Sbjct: 365 FSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYE 424 Query: 435 LGAYPASATRAGERQYLNARLGEIRATASRMSDTATLFQAMG 476 GA P ++ LN+ + R+ + L +A+G Sbjct: 425 GGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALG 466 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 130 bits (327), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 135/489 (27%), Positives = 224/489 (45%), Gaps = 33/489 (6%) Query: 3 AMPSIFTPRPLALAVALL----LSGCALGPSGEAPAMPQPAHYAVESTPATLGPQEARQK 58 +MP +PR L + AL+ L GC+L P+ E P P + Y PA + Q Sbjct: 5 SMPR--SPRLLHMCAALVMAGVLGGCSLAPTYERPQAPVESSY-----PADAAGRAELQA 57 Query: 59 FVLGQRAVPQWWQAYGSPQLSAWVDEALRNNHSLAQAERNLAGAQLQLRAQVGESLWPTI 118 LG W Q + +L + AL NN L A N+ A+ Q + + L P + Sbjct: 58 VNLG------WRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARAD-LLPNL 110 Query: 119 DAVGQTQRQRA------LGLPNIGPSTNVYNVFSGQIRASYTFDLFG-VQRFANSALAAQ 171 + G R R G P I +N Y V G +Y D FG V+ ++ALA+ Sbjct: 111 NLSGNAARTRTAASQSLTGQPTI---SNSYQV--GLAVPAYELDFFGRVRNLKDAALASY 165 Query: 172 VDAQSYQFDAARRTVAANVVISAINAASLRAQVAQTERLVALADADVEEMQRRLALGAVS 231 + + Q +A+ ++ A V + + S Q+ + + ++++ Q+RL +GA + Sbjct: 166 LSTEEAQ-KSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGAST 224 Query: 232 TDELLSARASAQSLRASLPGLKAQWQGARHALAVLMGRTPDAAPPDLDIAALTLPAEVPM 291 + + +S R + L+ Q A +AL +L+G+ PP ++ + ++P Sbjct: 225 ALDFRLTQTLVESARVAKTQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPA 284 Query: 292 TVPSDLLRQRPDILAADAALKVAAAQQAEATAEMFPSLSINASFGQSGFSWAKA-TSGVG 350 +PSDLL RPDI +A+ +L A A A A FP +S+ +FG + + SG G Sbjct: 285 GLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSG 344 Query: 351 AVWGLGASLTQPLFHGGALRAKRDAAKEAYLAAQSHYRQTVLNAFQNVADSLVALTQDAD 410 A W G SL+ P+F G A D A+ A Y + + AF+ VAD+LVA + Sbjct: 345 A-WSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDE 403 Query: 411 ALQASAAARTASEQSWRNAQRRAQLGAYPASATRAGERQYLNARLGEIRATASRMSDTAT 470 ++A A A ++ + R + G + +R +A+ I+A R+++ Sbjct: 404 QVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAID 463 Query: 471 LFQAMGVGL 479 L++A+G GL Sbjct: 464 LYKALGGGL 472 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.125 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,211,595 Number of extensions: 43748 Number of successful extensions: 303 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 263 Number of HSP's successfully gapped: 18 Length of query: 490 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 401 Effective length of database: 1,198,455 Effective search space: 480580455 Effective search space used: 480580455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)