BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0252.p (395 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309609|ref|YP_003773701.1| transport/efflux transmembrane ... 536 e-153 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 80 3e-16 gi|300313899|ref|YP_003777991.1| RND family efflux transporter M... 77 2e-15 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 64 1e-11 gi|300312743|ref|YP_003776835.1| membrane-fusion protein [Herbas... 62 7e-11 >gi|300309609|ref|YP_003773701.1| transport/efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 376 Score = 536 bits (1381), Expect = e-153, Method: Compositional matrix adjust. Identities = 303/381 (79%), Positives = 341/381 (89%), Gaps = 7/381 (1%) Query: 17 VKPVL-FVSPVLPVRRV-PLALLTVSFALLLSACSKPAPVAEAPREVVVLQAQERGGSLP 74 +KPV VSP RRV PL L ++ L+ACSKPAPVA+APREVVVLQAQ+RG + Sbjct: 1 MKPVSPSVSPGRARRRVYPLLALVIT----LAACSKPAPVADAPREVVVLQAQQRGQAGT 56 Query: 75 LQLPAEVQSRYVTSLSFRVAGQLVERRVHLGDVVRRGQVLARLDPADADQNAVSARAELA 134 L LPAEVQSRYVTS+SFR+AGQL ERRVHLGDVVR+GQVLARLDPADA+QNA +A+AELA Sbjct: 57 LHLPAEVQSRYVTSMSFRIAGQLAERRVHLGDVVRKGQVLARLDPADANQNASAAQAELA 116 Query: 135 SARQHLEAAEKQLQRDTEQAREQLISTQQLEQSQDAHAAALSQFKAAQARAAVAGNQRDY 194 SARQHL+AAEKQLQRD +QARE LIS QQLEQSQDAHAAALSQFKAAQARAAVAGNQRDY Sbjct: 117 SARQHLDAAEKQLQRDVQQAREALISPQQLEQSQDAHAAALSQFKAAQARAAVAGNQRDY 176 Query: 195 TTLVAQHDGVISAEQANTGDVLSAGQPVYALAWSGAMDVVTDVADRQLASLQPGAAATVT 254 TTLVAQHDGVISAEQANTGDVL+AGQPVY+LAWSG +DVVT+VADRQ+A+LQPGAAA VT Sbjct: 177 TTLVAQHDGVISAEQANTGDVLAAGQPVYSLAWSGQVDVVTEVADRQVATLQPGAAAVVT 236 Query: 255 LAALPGKHFQARVREISPAADPQSRTYRVKLTLEQPEPGVRLGMIGEVAITPHSDANAGQ 314 LAALPGK F +VRE+SPAAD QSRTYRVK+TLEQP+P VRLGM GEVAITP + ++A Sbjct: 237 LAALPGKTFTGKVREVSPAADAQSRTYRVKVTLEQPDPAVRLGMTGEVAITPAA-SSAAP 295 Query: 315 VMLLPATALFHQGGKPAVWIVGVEGKLELRPVIIAAYGERSIALSQGVTANDKVVVQGVH 374 V+LLPATALFHQG +PA+W+VG +G+LELRPV +AAYGERSI++SQGVTA+DKVVVQGVH Sbjct: 296 VLLLPATALFHQGDRPALWVVGEQGQLELRPVTVAAYGERSISVSQGVTASDKVVVQGVH 355 Query: 375 TLTVGEKVKPVAPLHAEDFAL 395 TLT GEKVKPVAPLHAEDFAL Sbjct: 356 TLTAGEKVKPVAPLHAEDFAL 376 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 80.1 bits (196), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 100/375 (26%), Positives = 155/375 (41%), Gaps = 21/375 (5%) Query: 29 VRRVPLALLTVSFALLLSACSKPAPVAEA----PREVVVLQAQERGGSLPLQLPAEVQSR 84 V R +TV+ A L AC + P A+ P EV V+ + ++ +LP V++ Sbjct: 4 VSRFTRLTMTVAIAAALVACGQ-KPAAQGGPMPPSEVSVVTIAPQRVAITNELPGRVEAF 62 Query: 85 YVTSLSFRVAGQLVERRVHLGDVVRRGQVLARLDPADADQNAVSARAELASARQHLEAAE 144 V + RVAG + +R G V+ GQVL ++DPA + SA+A LA A+ +L Sbjct: 63 RVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTT 122 Query: 145 KQLQRDTEQAREQLISTQQLEQSQDAHAAALSQFKAAQARAAVAGNQRDYTTLVAQHDGV 204 QR +S Q+ + + A A + A +A A Y T+ + G Sbjct: 123 LTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPISGR 182 Query: 205 ISAEQANTGDVLSAG-----------QPVYALAWSGAMDVVT---DVADRQLASLQPGAA 250 I G ++ G PVY + D++ +A QL S+ Sbjct: 183 IGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDN-K 241 Query: 251 ATVTLAALPGKHFQARVREISPAADPQSRTYRVKLTLEQPEPGVRLGMIGEVAITPHSDA 310 A+VTL G+ + + + T V L E P P RL + G A Sbjct: 242 ASVTLELENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPK-RLLLPGTYVRAKIEQA 300 Query: 311 NAGQVMLLPATALFHQGGKPAVWIVGVEGKLELRPVIIAAYGERSIALSQGVTANDKVVV 370 Q +L+P A+ G V +V E K+E RPV + + G+ D+V+V Sbjct: 301 VDEQALLVPQQAVVRDAGGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIV 360 Query: 371 QGVHTLTVGEKVKPV 385 +G+ + G VKPV Sbjct: 361 EGLQKVRPGATVKPV 375 >gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 406 Score = 77.4 bits (189), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 75/315 (23%), Positives = 147/315 (46%), Gaps = 10/315 (3%) Query: 75 LQLPAEVQSRYVTSLSFRVAGQLVERRVHLGDVVRRGQVLARLDPADADQNAVSARAELA 134 L LP +Q + +S R G L +G V++G++LA LD + DQ A A Sbjct: 84 LALPGTLQGFVQSPISARSGGYLKRWTKDIGAEVKKGELLAELDTPEIDQQLTQALAARE 143 Query: 135 SARQHLEAAEKQLQRDTEQAREQLISTQQLEQSQDAHAAALSQFKAAQARAAVAGNQRDY 194 A +E A + R ++ +S Q+L++ + A A A + AA A + Sbjct: 144 QAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASANLAAADANVGRLRQVEGF 203 Query: 195 TTLVAQHDGVISAEQANTGDVLS--AGQPVYALAWSGAMDVVTDVADRQLASLQPGAAAT 252 +VA G+I+ + GD++ AG+ ++ L+ + + V +V ++PG T Sbjct: 204 KRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNVPQSYANLVKPGQQVT 263 Query: 253 VTLAALPGKHFQARVREISPAADPQSRTYRVKLTLEQPEPGVRLGMIGEVA--ITPHSDA 310 VT + L G+ F+ +V + A D +R+ +V++TL + + G +VA I P Sbjct: 264 VTQSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSNADHALLPGAFVQVALPIKP---- 319 Query: 311 NAGQVMLLPATALFHQGGKPAVWIVGVEGKLELRPVIIAAYGERSIALSQGVTANDKVVV 370 +G +++ T +F + G V V ++ L+ + + +++ + G+ ++ +++ Sbjct: 320 -SGALLVAADTLMFRRDGT-LVAAVDPHNQVRLKKLRLGRNFGQTVEVLDGLQGDEHLIL 377 Query: 371 QGVHTLTVGEKVKPV 385 L G++V+P Sbjct: 378 NPSDALAEGDQVEPT 392 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 64.3 bits (155), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 89/381 (23%), Positives = 163/381 (42%), Gaps = 50/381 (13%) Query: 31 RVPLALLTVSFALLLSACSK----PAPVAEAPREVVVLQAQERGGSLPLQLPAEVQSRYV 86 R+ L+L+ V+ +L+ACS+ P P A+A VV++AQ + +L +LP + Sbjct: 20 RLALSLILVA---VLAACSRSGDQPKPTAQAEVGYVVVKAQRQ--ALTTELPGRTTAYLA 74 Query: 87 TSLSFRVAGQLVERRVHLGDVVRRGQVLARLDPADADQNAVSARAELASARQHLEAAEKQ 146 + +V G + +R G V+ GQ L ++DPA SARA L A L +++ + Sbjct: 75 ADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEATLASSKLK 134 Query: 147 LQRDTEQAREQLISTQQLEQSQDAHAAALSQFKAAQARAAVAGNQRDYTTLVAQHDGVIS 206 +R E + +S Q + +Q + + +A A YT +++ G I Sbjct: 135 AERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIE 194 Query: 207 AEQANTGDVLSAGQ-----------PVYALAWSGAMDVVTDVAD-----RQLASLQ---- 246 G +++A Q P+Y +DV D R+LAS Q Sbjct: 195 KSAVTPGALVTASQTTALTTVQQIDPIY-------VDVTQSTVDLLRLRRELASGQIRKS 247 Query: 247 PGAAATVTLAALPGKHFQ--ARVREISPAADPQSRTYRVKLTLEQPE----PGVRLGMIG 300 AA V + G ++ ++ + D + ++ + P+ PG + + Sbjct: 248 GENAAQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVL 307 Query: 301 EVAITPHSDANAGQVMLLPATALFH-QGGKPAVWIVGVEGKLELRPVIIAAYGERSIALS 359 E + H+ +L P A+ Q G ++G +GK++ R V +A + +S Sbjct: 308 EEGVDDHA-------LLAPQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVS 360 Query: 360 QGVTANDKVVVQGVHTLTVGE 380 +G+ DK++V G+ + G+ Sbjct: 361 RGLKEGDKLIVDGLQKVRTGD 381 >gi|300312743|ref|YP_003776835.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 62.0 bits (149), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 86/303 (28%), Positives = 134/303 (44%), Gaps = 28/303 (9%) Query: 92 RVAGQLVERRVHLGDVVRRGQVLARLDPA-----DADQNAVSAR--AELASARQHLEAAE 144 +VAGQ+ + V +G+ V+ G++LA + PA + A AR AELA L+ A+ Sbjct: 89 KVAGQISDVDVVVGETVKAGKLLATVAPAVQSSRIENNRATLARLKAELAGQNAQLDFAQ 148 Query: 145 KQLQRDTEQAREQLISTQQLEQSQDAHAAALSQFKAA-----QARAAVAGNQ--RDYTTL 197 Q QR T+ E + E S+ AA ++ A Q AA+ ++ + T + Sbjct: 149 LQFQRQTQLKAENATREESYESSRMNMYAAAARVDATNAQIQQTEAAIREDEAVQKQTRI 208 Query: 198 VAQHDGVISAEQANTGDVLSAGQP-VYALAWSGAMDVVTDVADRQLASLQPGAAATVTLA 256 A G I A G +++A Q + +A M V VA+ + LQ G A T Sbjct: 209 EAPVSGTIVTLNARPGQMVAANQEALMRIADLSRMTVQVPVAEEDVTRLQKGMTAYFTTP 268 Query: 257 ALPGKHFQARVREIS--PAADP----QSRTYRVKLTLEQPEPGVRLGMIGEVAITPHSDA 310 PGK + ++R+I P D + Y V + P + GM +V A Sbjct: 269 GYPGKRWSGKLRQIMLLPTDDSGRQGKKAFYTVFFDVANPSRELMSGMSADVYFVL---A 325 Query: 311 NAGQVMLLPATALFHQG--GKPAVWIVGVEGKLELRPVIIAAY-GERSIALSQGVTANDK 367 A V +P T + G G V +V +GKLE R + I ER+ LS G+ ++ Sbjct: 326 RAEHVPTIPRTLVTKPGMDGTQTVKVVLADGKLETRKIKIGIRDNERAQVLS-GLKEGEQ 384 Query: 368 VVV 370 V++ Sbjct: 385 VLL 387 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,054,169 Number of extensions: 41838 Number of successful extensions: 244 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 236 Number of HSP's successfully gapped: 12 Length of query: 395 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 308 Effective length of database: 1,207,925 Effective search space: 372040900 Effective search space used: 372040900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)