BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0239.p (450 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309354|ref|YP_003773446.1| 4-aminobutyrate aminotransferas... 436 e-123 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 143 2e-35 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 143 3e-35 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 130 3e-31 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 124 1e-29 >gi|300309354|ref|YP_003773446.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 436 bits (1121), Expect = e-123, Method: Compositional matrix adjust. Identities = 221/434 (50%), Positives = 290/434 (66%), Gaps = 4/434 (0%) Query: 11 VNAYIPGR-ADVGPVTGALIERRDALLGPAYRLMYEHPLHIVRGEGVWLIDPQGRRYLDV 69 +NA+ P + P T ALIERR G L Y P+H V GVWL D G YLD Sbjct: 10 LNAFNPQSGSTTAPATRALIERRRNTFGDGVPLFYRDPVHFVSASGVWLRDQDGLEYLDA 69 Query: 70 YNNVTSLGHCHPAVTEAICTQVQTLATNTRYLHETILELAERLLATVSETALAHLMLTCT 129 YNNV S+GHCHP V EA+ Q TL T+TRYL + + AERLL T+ L+ ++ T + Sbjct: 70 YNNVPSVGHCHPRVVEAVSKQAATLNTHTRYLSDVVTSYAERLLGTLPRE-LSKVLFTSS 128 Query: 130 GSEANDLAYRIAKVRTGGVGVIVTDTAYHGITDAVSQFSPSLGTTVNLGAHVRLVPAPRS 189 G+E+ DLA R+A++ TGG G IVT AYHG + AV++ +P+ G V +G +VRLV AP Sbjct: 129 GTESVDLAMRLARLYTGGSGFIVTRFAYHGHSTAVAEITPAFGPGVPIGINVRLVDAPDG 188 Query: 190 YHAEGADLGERFTRDVEAAIADLKRHGIQPAALIVDSLFTSDGILPGPEGFLKGAVEAIK 249 Y + A +G +F +DV AAIAD++RHGI+ A LI+D++ +SDG+ P GFLK A+ +K Sbjct: 189 YRQD-APVGIKFAQDVAAAIADMERHGIRFAGLILDTVCSSDGLFVDPPGFLKDAIATVK 247 Query: 250 RAGGLFIADEVQPGFGRTGEHMWGFQRHGVIPDIVTMGKPMGNGQPVAGLLATADVLQEF 309 +AGG+FIADEVQPGFGRTGE MWGF+RH ++PDIV MGKPMGNG P+A + AD++Q F Sbjct: 248 KAGGVFIADEVQPGFGRTGEGMWGFERHRIVPDIVVMGKPMGNGMPIAATVTRADIMQAF 307 Query: 310 GQRSRYFNTFGGNTVSCAAALAVLKTIENEALIAHAQQVGSVLLNGLSALAERHAAIGDV 369 +RS YFNTFGGNTV CAAA AVL IE+E LI +A++ G+ L GL L +H IGDV Sbjct: 308 TERSGYFNTFGGNTVCCAAASAVLDVIESEGLINNAKETGASLKLGLEQLKGQHQCIGDV 367 Query: 370 RGAGMFVGVELVTDRHTRAPDRSLTSEVVNRMRQKGVLLSACAMGHNVLKIRPPLVLTAQ 429 R G++ VE V T+ D + VVN +RQK +L+S C N+LKIRPPL + Q Sbjct: 368 RAIGLYAAVEFVLP-DTKTADTDMALHVVNTLRQKRILISTCGPAGNILKIRPPLQFSRQ 426 Query: 430 QAGLVVQALDESLN 443 L+V A+D+ L+ Sbjct: 427 HCDLLVTAIDDVLH 440 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 143 bits (361), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 124/436 (28%), Positives = 204/436 (46%), Gaps = 30/436 (6%) Query: 22 GPVTGALIE---RRDALLGPAYRLMYEHPLHIVRGEGVWLIDPQGRRYLDVYNNV--TSL 76 GP T A +E R +++ R+ P+ + + GV DP G ++D+ V +S+ Sbjct: 24 GPKTAAALELSARTESMARGGGRM----PIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSV 79 Query: 77 GHCHPAVTEAICTQVQTLATNTRYLHETILELAERLLATVSETALAHLM--LTCTGSEAN 134 G C+P V EAI Q ++L + ELA ++ + + + T GS+A Sbjct: 80 GRCNPRVVEAIRKQSESLMHSMEVNSSKRTELAAKISEIMPDGLRGDCITFFTQGGSDAL 139 Query: 135 DLAYRIAKVRTGGVGVIVTDTAYHGITDAVSQFSPSLGTTVNLGAHVRLV---PAPRSYH 191 + A + AK TG +I YHGI +A + + G + V P P +Y Sbjct: 140 EAAVKFAKRVTGRHQIIAFHGGYHGIWNASNALTTGTAYRKGFGPFMGGVIHAPYPYAYR 199 Query: 192 AEGADLGERFTRDVEAAIADLKRH-----GIQPAALIVDSLFTSDGILPGPEGFLKGAVE 246 D + + D + AA+IV+ + G +P FL+ + Sbjct: 200 FP-FDTSHKSAEQIAGEYVDYLLNTPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRK 258 Query: 247 AIKRAGGLFIADEVQPGFGRTGEHMWGFQRHGVIPDIVTMGKPMGNGQPVAGLLATADVL 306 A R+G L I DEVQ G GRTG+ MW + GV PD++T GK +G P+AGL+ +D+ Sbjct: 259 ACDRSGALLIVDEVQAGAGRTGK-MWAVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLA 317 Query: 307 QEFGQRSRYFNTFGGNTVSCAAALAVLKTIENE--ALIAHAQQVGSVLLNGLSALAERHA 364 + S+ NTF N++S A AL + +++ L+ A +G + + Sbjct: 318 AKIPDGSQP-NTFAANSISAAVALTNISILQDPRLDLVNRAHTLGLEAQERIRSF--NSP 374 Query: 365 AIGDVRGAGMFVGVELVTDRHTRAP-DRSLTSEVVNRMRQKGVLLSACAMGHNVLKIRPP 423 +G+VRG G+ +G+ELV +R TR P R ++++ + GVL+ C NV+++ P Sbjct: 375 WVGEVRGRGLMIGIELVENRETREPLSREKLGKLMDYVVGHGVLMIPCGRYTNVMRVMPS 434 Query: 424 LVLTAQQAGLVVQALD 439 L + L+ + LD Sbjct: 435 LTIPRS---LMFKGLD 447 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 143 bits (360), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 115/390 (29%), Positives = 179/390 (45%), Gaps = 29/390 (7%) Query: 52 RGEGVWLIDPQGRRYLDVYNNVTSL--GHCHPAVTEAICTQVQTLATNTRYL---HETIL 106 R L D +GRR++D + L GH HP + +A+ Q+ T+T Y + + + Sbjct: 32 RAANAELWDVEGRRFIDFAAGIAVLNTGHRHPKLLDAMRAQMDKF-THTAYQIVPYASYV 90 Query: 107 ELAERLLATVSETALAHLMLTCTGSEANDLAYRIAKVRTGGVGVIVTDTAYHG------- 159 ELAER+ TG+EA + A +IA+ TG GVI +HG Sbjct: 91 ELAERINRLTPGNYPKKTAFFSTGAEAVENAIKIARAHTGRPGVIAFAGGFHGRTMMGMA 150 Query: 160 ITDAVSQFSPSLGTTVNLGAHVRLVPAPRSYHAEGADLGERFTRDVEAAIADLKRHGIQP 219 +T V+ + G V P P + H ++ D A+ L + I+ Sbjct: 151 LTGKVAPYKLGFGP---FPGDVFHAPYPSALHGITSE-------DALEAVKGLFKSDIEA 200 Query: 220 ---AALIVDSLFTSDGILPGPEGFLKGAVEAIKRAGGLFIADEVQPGFGRTGEHMWGFQR 276 AA+I++ + G P F++G G L IADEVQ G+GRTG+ ++ + Sbjct: 201 KRVAAIILEPVQGEGGFYAAPADFMRGLRALCDEHGILLIADEVQSGYGRTGK-LFAMEH 259 Query: 277 HGVIPDIVTMGKPMGNGQPVAGLLATADVLQEFGQRSRYFNTFGGNTVSCAAALAVLKTI 336 + V+PD++TM K + G P++ + A+++ + T+ GN ++ A+ALAVL + Sbjct: 260 YDVLPDLMTMAKSLAGGMPLSAVNGRAEIM-DAPAPGGLGGTYAGNPLAIASALAVLDVM 318 Query: 337 ENEALIAHAQQVGSVLLNGLSALAERHAAIGDVRGAGMFVGVELVTDRHTRAPDRSLTSE 396 E E L+ Q++G L L L I +VRG G V VE D T PD T + Sbjct: 319 EEEQLVTRGQRLGDKLQEHLKELRSSVPQIAEVRGVGAMVAVEF-ADPATGKPDAEYTKK 377 Query: 397 VVNRMRQKGVLLSACAMGHNVLKIRPPLVL 426 V G+LL C NV++ PL + Sbjct: 378 VQQHALNNGLLLLTCGSYGNVIRFLFPLTI 407 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 130 bits (326), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 127/427 (29%), Positives = 204/427 (47%), Gaps = 43/427 (10%) Query: 51 VRGEGVWLIDPQGRRYLDVYNN--VTSLGHCHPAVTEAICTQVQTLA-TNTRYLHETILE 107 V GEG+ LID G+RY+D V+ LGH HP V EAI QV LA +T + E Sbjct: 17 VAGEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYAHTSFFTTAPAE 76 Query: 108 LAERLLATVSETALAHLMLTCTGSEANDLAYRIAKVRTGGVG------VIVTDTAYHGIT 161 +LA + +L H+ GSEA + A ++A+ VG +I +YHG T Sbjct: 77 ELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQPQRRHIIARRQSYHGNT 136 Query: 162 DAV----------SQFSPSLGTTVNLGAHVRLVPAPRSYHAEGADLG--ERFTRDVEAAI 209 F P L HV A R+ +D+ +R ++E I Sbjct: 137 LGALAIGGNAWRREMFMPMLIEA----HHVSPCYAYRNRADGESDVQYVQRLADELEQKI 192 Query: 210 ADLKRHGIQPAALIVDSLF-TSDGILPGPEGFLKGAVEAIKRAGGLFIADEVQPGFGRTG 268 L Q A + +++ + G +P + + + G L I DEV G GRTG Sbjct: 193 LSLGAD--QVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEVMSGMGRTG 250 Query: 269 EHMWGFQRHGVIPDIVTMGKPMGNG-QPVAGLLATADVLQEFGQRSRYF---NTFGGNTV 324 +++ + GV+PDIV + K +G G QP+ ++ + + + S +F +T+ G+ Sbjct: 251 -YLFACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQHGHTYIGHAT 309 Query: 325 SCAAALAVLKTIENEALIAHAQQVGSVLLNGLSALAERHAAIGDVRGAGMFVGVELVTDR 384 +CAAA+AV KTI+ E L+ + +Q G L + L A +GD+RG G+FVGVELV +R Sbjct: 310 ACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGRGLFVGVELVAER 369 Query: 385 HTR---APDRSLTSEVVNRMRQKGVLLSACA------MGHNVLKIRPPLVLTAQQAGLVV 435 ++ +PD + V ++G+L+ G ++L + PP + ++ +V Sbjct: 370 SSKLPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHIL-LAPPFIASSNDISEIV 428 Query: 436 QALDESL 442 Q L +++ Sbjct: 429 QRLSDAV 435 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 118/425 (27%), Positives = 188/425 (44%), Gaps = 34/425 (8%) Query: 47 PLHIVRGEGVWLIDPQGRRYLDVYNNVTSL--GHCHPAVTEAICTQVQTLATNTRYLH-- 102 PL + G+G +L D Q ++ LD ++ + + G+ +V A Q+Q L T Y H Sbjct: 29 PLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQESVIRAATEQMQRLPYATGYFHFA 88 Query: 103 -ETILELAERLLATVSETALAHLMLTCTGSEANDLAYRIAKVRTGGVG------VIVTDT 155 E + LA++L+ +S +L H+ T GS+A D A R +G I Sbjct: 89 SEPAILLAKKLV-DISPASLQHVYFTLGGSDAVDAAIRYITHYHHSIGKPGKQHFISLQR 147 Query: 156 AYHGITDAVSQFS--PSLGTTVNLGAHVR-LVPAPRSYHAEGADLGERFTRDVEA---AI 209 YHG + + + P+ NL + +P+P Y +E V+A + Sbjct: 148 GYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLPSPYPYRSELQGDEAIIAASVQALRDKV 207 Query: 210 ADLKRHGIQPAALIVDSLFTSDGILPGPEGFLKGAVEAIKRAGGLFIADEVQPGFGRTGE 269 A+L + AA + + S G++ P+G+LK A LF+ DEV GFGRTG Sbjct: 208 AELGADNV--AAFFCEPIQGSGGVVVPPKGWLKAMQLACNELDILFVVDEVITGFGRTGP 265 Query: 270 HMWGFQRHGVIPDIVTMGKPMGNGQPVAGLLATADVLQE-------FGQRSRYFNTFGGN 322 M+ V PD++TM K + G G + +D + + T+ + Sbjct: 266 -MFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDRVYNGIADGAPMSTVVGHGQTYSAH 324 Query: 323 TVSCAAALAVLKTIENEALIAHAQQVGSVLLNGLSALAERHAAIGDVRGAGMFVGVELVT 382 VS A AL VL+ E L+ + +++ V GL AL H +GD R G+ +ELV Sbjct: 325 PVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGLRALLA-HPLVGDARSRGLLGALELVA 383 Query: 383 DRHTR---APDRSLTSEVVNRMRQKGVLLSACAMGHNVLKIRPPLVLTAQQAGLVVQALD 439 D+ T+ AP+ L + + G++ A G N+L P L T + L+ L Sbjct: 384 DKQTKARFAPELKLHDRIFQAAYRHGLVFR--AFGDNILGFAPALCYTQEDFALMFARLK 441 Query: 440 ESLNE 444 L+E Sbjct: 442 AILDE 446 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,487,045 Number of extensions: 68738 Number of successful extensions: 251 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 225 Number of HSP's successfully gapped: 9 Length of query: 450 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 362 Effective length of database: 1,203,190 Effective search space: 435554780 Effective search space used: 435554780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)