BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0234.p (596 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 1223 0.0 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 522 e-149 gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 465 e-132 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 134 2e-32 gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbasp... 105 1e-23 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 1223 bits (3164), Expect = 0.0, Method: Compositional matrix adjust. Identities = 584/596 (97%), Positives = 591/596 (99%) Query: 1 MPQYNAPLRDMQFVLHELLKVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHS 60 MPQYNAPLRDMQFVLHELL VEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHS Sbjct: 1 MPQYNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHS 60 Query: 61 GDREGCHYDRDSKTVTTPKGFKEAYQQYVQAGWAALSCDPEFGGQGLPLTINNSFYEMLN 120 GDREGCHYDR+SKTVTTPKGFK+AYQQYV+AGWAALSCDPEFGGQGLPLTINNSFYEMLN Sbjct: 61 GDREGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLN 120 Query: 121 SSNQAWTMYPGLSHGAYECLHAHGSDEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLR 180 SSNQAWTMYPGLSHGAYECLHAHGS+EQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLR Sbjct: 121 SSNQAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLR 180 Query: 181 SKAEPQADGSYKITGNKIFISAGENDVSGNIIHLVLARLPDAPEGSKGISLFLVPKFLPN 240 SKAEPQADGSYKITGNKIFISAGENDVS NIIHLVLARLPDAPEGSKGISLFLVPKFLPN Sbjct: 181 SKAEPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPN 240 Query: 241 TDGSLGARNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLG 300 DGSLGARNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLG Sbjct: 241 ADGSLGARNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLG 300 Query: 301 VGMQGLGLTEVAYQNALVYAKDRLQMRSLSGVKAADKPADPIIVHPDVRRMLLTAKAFAE 360 VGMQGLGLTEVAYQNALVYAKDRLQMRSLSG+KAADKPADPIIVHPDVRRMLLTAKAFAE Sbjct: 301 VGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVHPDVRRMLLTAKAFAE 360 Query: 361 GGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGG 420 GGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGG Sbjct: 361 GGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGG 420 Query: 421 HGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEEH 480 HGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEEH Sbjct: 421 HGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEEH 480 Query: 481 GTDESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPYLRVVGHLVFSYLFAR 540 GTDESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPYLRVVGHLVFSYLFAR Sbjct: 481 GTDESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPYLRVVGHLVFSYLFAR 540 Query: 541 MAKIALEKQDSGDAFYTAKLATARFYFARLLPETAMLIRQARSGAGNLMALDAQLF 596 MAKIALEKQDSGD+FY AKLATARFYFARLLPETAMLIRQARSGA NLMALDA+LF Sbjct: 541 MAKIALEKQDSGDSFYAAKLATARFYFARLLPETAMLIRQARSGAANLMALDAELF 596 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 522 bits (1345), Expect = e-149, Method: Compositional matrix adjust. Identities = 272/581 (46%), Positives = 363/581 (62%), Gaps = 27/581 (4%) Query: 3 QYNAPLRDMQFVLHELLKVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHSGD 62 Y PLRD FVL E+L+ +V + LP HA+ + +L+ QVLEE G+FA+ V+ PLN GD Sbjct: 4 HYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNGIGD 63 Query: 63 REGCHYDRDSKTVTTPKGFKEAYQQYVQAGWAALSCDPEFGGQGLPLTINNSFYEMLNSS 122 REGC + + TVTTP GF+ AY Q+ GW AL+C PE GGQGLP ++ + YEML+++ Sbjct: 64 REGCRFK--NGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAA 121 Query: 123 NQAWTMYPGLSHGAYECLHAHGSDEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSK 182 N WTMYPGL HGAY CL AHGS + YL K+ SGEW TMCLTEA G+DLG LR++ Sbjct: 122 NHGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRTR 181 Query: 183 AEPQADGSYKITGNKIFISAGENDVSGNIIHLVLARLPDAPEGSKGISLFLVPKFLPNTD 242 A PQ DGSY+++G+KIFIS GE+D++ NI+HLVLARLPDAP GSKG+SLFL P+ L + Sbjct: 182 AVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDD-- 239 Query: 243 GSLGARNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLGVG 302 G+RN + C IE KMGIHG+ATC ++ + A WL+G+ +GL AMFV MNAARL VG Sbjct: 240 ---GSRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVG 296 Query: 303 MQGLGLTEVAYQNALVYAKDRLQMRSLSGVKAADKPADPIIVHPDVRRMLLTAKAFAEGG 362 QGLGL E AYQ A YA++R Q R+ D I+ HP V+++L+ + EG Sbjct: 297 AQGLGLAEAAYQRASAYARERRQSRA-----PGSSGNDLIVHHPAVQKLLMEQRCRIEGA 351 Query: 363 RAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGGHG 422 R T + L LD P+ +R + + +TPV+KAFLTD + S LQV+GGHG Sbjct: 352 RLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHG 411 Query: 423 YIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEEHGT 482 Y+ E G+EQ++RDARI MIYEGTN IQ++D L RK+L D G +L F ++A + E Sbjct: 412 YVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVRADIAEASG 471 Query: 483 DESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPY------LRVVGHLVFSY 536 + +G+ V KL + S A A A+PY LR+ GH+ + Sbjct: 472 LGEQARL------LGEAVDKLRI---FASRIAAAAAQAPALPYYVADEMLRLCGHVALGW 522 Query: 537 LFARMAKIALEKQDSGDAFYTAKLATARFYFARLLPETAML 577 ++ R + AL +Y K A ++FA + E L Sbjct: 523 MWLRAGRRALSAMAQDPLWYGGKRDAACYHFAFVFAEVQQL 563 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 465 bits (1196), Expect = e-132, Method: Compositional matrix adjust. Identities = 258/600 (43%), Positives = 361/600 (60%), Gaps = 15/600 (2%) Query: 4 YNAPLRDMQFVLHELLKVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHSGDR 63 Y AP++DM FV++EL + V LP D E + +LEE KF S+V+ PLNHS D+ Sbjct: 3 YTAPVKDMLFVMNELAGLAAV-NALPGCEDATPETAEAILEENAKFCSEVVAPLNHSADK 61 Query: 64 EGCHYDRDSKTVTTPKGFKEAYQQYVQAGWAALSCDPEFGGQGLPLTINNSFYEMLNSSN 123 E + D K T+P GFKEA++Q+ +AGW + EFGGQGLP + EMLNS+N Sbjct: 62 EPSSW-ADGKVTTSP-GFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSAN 119 Query: 124 QAWTMYPGLSHGAYECLHAHGSDEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSKA 183 ++ + P L+ G E L G+ +QK+ ++ +SG+WTGTM LTE G+DL L+R++A Sbjct: 120 LSFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRA 179 Query: 184 EPQADGSYKITGNKIFISAGENDVSGNIIHLVLARLPDAPEGSKGISLFLVPKFLPNTDG 243 P+ DG+YK+ G KIFI+ GE+D++ NI+HLVLAR P+APEG KGISLF+VPKFL N DG Sbjct: 180 VPEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADG 239 Query: 244 SLGARNPIFCGAIEEKMGIHGNATCQMNL---DGATGWLIGQPHKGLQAMFVFMNAARLG 300 SLGARN + C +IE K+GI + T + GA G L+G+ ++GL+ MF+ MNAAR Sbjct: 240 SLGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFA 299 Query: 301 VGMQGLGLTEVAYQNALVYAKDRLQMRSLSGVKAADKPADPIIVH-PDVRRMLLTAKAFA 359 VG+QGL ++E AYQ A+ YA+DR+Q R L+G P I+H PDVRRML++ +A Sbjct: 300 VGIQGLSVSERAYQKAVAYARDRIQSRDLAG-----SPGPVAIIHQPDVRRMLMSMRAQI 354 Query: 360 EGGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYG 419 E RA + A D DE RK + L P+VK ++T+ + TS +QV+G Sbjct: 355 EAARALSYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHG 414 Query: 420 GHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEE 479 G G+I E G QY RDARI IYEGT IQ+ DL+GRK D GA K AQ++ V E Sbjct: 415 GMGFIEETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRKTVSE 474 Query: 480 HGTDESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPYLRVVGHLVFSYLFA 539 D + L + D + ++ + ++ A +VPYL++ G ++ + A Sbjct: 475 LQGDAQLQPIAARLGEAADALEQVVDYVVANVKSDIKAVFAGSVPYLKMAGVVLGGWQMA 534 Query: 540 RMAKIALEKQDSGDA---FYTAKLATARFYFARLLPETAMLIRQARSGAGNLMALDAQLF 596 R A ++ K +G+ FY AKLATARFY ++LP+ G +MAL + F Sbjct: 535 RAAAVSAAKLKAGEGDGKFYEAKLATARFYADQILPQALAYRTAIVDGGAAVMALAEEQF 594 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 134 bits (337), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 119/418 (28%), Positives = 183/418 (43%), Gaps = 59/418 (14%) Query: 49 FASQVLFPLNHSGDREGCHYDRDSKTVTTPKGFKEAYQQYVQAGWAALSCDPEFGGQGLP 108 FA + L P DR T P EA ++ + G + ++GG G+ Sbjct: 17 FAQERLLPFAADWDRNH----------TFPA---EALKELAELGAMGMCVPEQWGGAGMD 63 Query: 109 -LTINNSFYEMLNSSNQAWTMYPGLSHGAYECLHAHGSDEQKQLYLPKLVSGEWTGTMCL 167 +++ + E+ T+ + A +GS QK+ +L L GE G CL Sbjct: 64 YMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFCL 123 Query: 168 TEAHCGTDLGLLRSKAEPQADGSYKITGNKIFISAGENDVSGNIIHLVLARLPDAPEGSK 227 TE H G+D +R++AE D + + G K FI++G++ +G + +V A + D G K Sbjct: 124 TEPHTGSDAAAIRTRAERDGD-DFILNGTKQFITSGKH--AG--VAIVFA-VTDRSAGKK 177 Query: 228 GISLFLVPKFLPNTDGSLGARNPIFCGAIEEKMGIHGNATCQMNLDGA---TGWLIGQPH 284 GIS FLVP P G EEKMG H + T Q+ LD L+G+ Sbjct: 178 GISCFLVPCDTPG----------FVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEG 227 Query: 285 KGLQAMFVFMNAARLGVGMQGLGLTEVAYQNALVYAKDRLQMRSLSGVKAADKPADPIIV 344 +G + + A R+G+ Q +G+ A++ A+ YA+ R GV PII Sbjct: 228 EGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESF----GV--------PIIE 275 Query: 345 HPDVRRMLLTAKAFAEGGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLT 404 H V L + R + A +L KD + K F + Sbjct: 276 HQAVNFRLADMNTLLDAAR-LMVWRAAQL------------KDQGRPCLKEASMAKMFAS 322 Query: 405 DNAWMATSECLQVYGGHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDN 462 + A S+ +Q++GG GY +++ +E+ RD RI IYEG N IQ L ++GR I D Sbjct: 323 EAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYEGANDIQRL-VIGRAIASDQ 379 >gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 105 bits (262), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 85/299 (28%), Positives = 141/299 (47%), Gaps = 47/299 (15%) Query: 165 MCLTEAHCGTDLGLLRSKA------EPQADG---SYKITGNKIFISAGENDVSGNIIHLV 215 M +TE G+D+ RS A +P DG +Y ++G+K F SA D HL+ Sbjct: 178 MGMTEKQGGSDV---RSNASIAMPVDPLNDGRGAAYLLSGHKWFFSAPMCDA-----HLM 229 Query: 216 LARLPDAPEGSKGISLFLVPKFLPNTDGSLGARNPIFCGAIEEKMGIHGNATCQMNLDGA 275 LAR + G+S F VP++LP+ G RNPI +++K+G N++ ++ + A Sbjct: 230 LARTEN------GLSCFFVPRWLPD-----GTRNPILIQRLKDKLGNRSNSSSEVEFEEA 278 Query: 276 TGWLIGQPHKGLQAMFVFMNAARLGVGMQGLGLTEVAYQNALVYAKDRLQMRSLSGVKAA 335 G ++G +G+ + N RL + L + ALV A + RS G + A Sbjct: 279 MGIMVGDEGRGIPTIIEMANHTRLDCIIGSAAL----MRQALVQAIHHARHRSAFGRRLA 334 Query: 336 DKPADPIIVHPDVRRMLLTAKAFAEGGRAFTSYVALELDKELTHPDEQVRKDSADIVALL 395 ++P +R +L +E VA D PD+ +++ I+ Sbjct: 335 EQPL--------MRAVLADLALESEAATMLMLRVAHAFDA----PDDPLQRAWKRII--- 379 Query: 396 TPVVKAFLTDNAWMATSECLQVYGGHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLL 454 TP K ++ T EC++V+GG+GY+ M + R+A +N I+EG+ + LD+L Sbjct: 380 TPAAKFWICKRTLEFTGECMEVWGGNGYVETAPMARLYREAPVNSIWEGSGNVMCLDVL 438 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,905,439 Number of extensions: 83680 Number of successful extensions: 227 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 209 Number of HSP's successfully gapped: 8 Length of query: 596 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 505 Effective length of database: 1,188,985 Effective search space: 600437425 Effective search space used: 600437425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)