BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0231.p (124 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309593|ref|YP_003773685.1| heat shock protein 15 [Herbaspi... 240 2e-65 >gi|300309593|ref|YP_003773685.1| heat shock protein 15 [Herbaspirillum seropedicae SmR1] Length = 124 Score = 240 bits (612), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 116/124 (93%), Positives = 122/124 (98%) Query: 1 MDTTRIDKWCWAARFFKTRSQATEAVDRGRIKLNGERTKPAHNVKTGDRLQIDNGSTEWE 60 MDTTRIDKWCWAARFFKTRSQATEAV+RGRI+LNGERTKPAHNVK GDRL +DNG+TEWE Sbjct: 1 MDTTRIDKWCWAARFFKTRSQATEAVERGRIRLNGERTKPAHNVKAGDRLDVDNGATEWE 60 Query: 61 VLVTGIADKRGSAAIAATLYQESEESLARRAAQAERHRLFREPSAEIKGRPTKRDRRLLD 120 VLVTGIADKRGSAAIAATLYQE+EES+ARRAAQAERHRLFREPSAEIKGRPTKRDRRLLD Sbjct: 61 VLVTGIADKRGSAAIAATLYQETEESIARRAAQAERHRLFREPSAEIKGRPTKRDRRLLD 120 Query: 121 RSSS 124 RSSS Sbjct: 121 RSSS 124 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 326,490 Number of extensions: 11660 Number of successful extensions: 46 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 46 Number of HSP's successfully gapped: 1 Length of query: 124 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 50 Effective length of database: 1,269,480 Effective search space: 63474000 Effective search space used: 63474000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)