BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.0222.p
         (391 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309586|ref|YP_003773678.1| FMN-dependent L-lactate dehydro...   687   0.0  
gi|300311906|ref|YP_003775998.1| L-lactate dehydrogenase [Herbas...   256   3e-69

>gi|300309586|ref|YP_003773678.1| FMN-dependent L-lactate
           dehydrogenase [Herbaspirillum seropedicae SmR1]
          Length = 413

 Score =  687 bits (1773), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 363/391 (92%), Positives = 376/391 (96%)

Query: 1   LERRLYTGPDLQRAQSIEELRAIARKRVPNFCFEYVEGGAEEEISLRRNREVFSHIGFLP 60
           L+RRLY G D+QRAQSIEELRA+ARKRVPNFCFEYVEGGAEEEISLR NREVF+ IGFLP
Sbjct: 21  LKRRLYAGADVQRAQSIEELRAMARKRVPNFCFEYVEGGAEEEISLRHNREVFTRIGFLP 80

Query: 61  RTLVDVSVRRQARKLFGQEIASPFLIGPTGFSGLLAREGDIAMASAAAGAGIPFVLTNVS 120
           RTLVDVSVRRQ R+LFGQ+IASPFLIGPTGFSGLLAREGD+AMASAAA AG+PFVLTNVS
Sbjct: 81  RTLVDVSVRRQGRRLFGQDIASPFLIGPTGFSGLLAREGDVAMASAAASAGVPFVLTNVS 140

Query: 121 TTSLEEVVRRSGAQVWQQVYLYRDRAFVASVAQRAQAAGIDTLVLTTDSAVYGKREWDVR 180
           TTSLEEVVRRSGAQVWQQVYLYRDRAFVASVAQRAQAAGI  LVLTTDSAVYGKREWD R
Sbjct: 141 TTSLEEVVRRSGAQVWQQVYLYRDRAFVASVAQRAQAAGIGVLVLTTDSAVYGKREWDAR 200

Query: 181 NFSSPRRLDWRNKLDVLCHPRWLIDILYPHGFPRFANLGDLLPADQTSVRGAAASILGQS 240
           NFSSPRRLDWRNKLDVL HPRWLIDILYPHGFPRFANLGDLLP DQTSVRGAAA+ILGQS
Sbjct: 201 NFSSPRRLDWRNKLDVLRHPRWLIDILYPHGFPRFANLGDLLPPDQTSVRGAAAAILGQS 260

Query: 241 LSPALDWRDVQWLRGIWNGKLVLKGVMQVQDAQRAVALGMDGIVLSNHGGRQLDGALSTM 300
           LS ALDW DVQWLRGIW GKLVLKGVMQV+DAQRAVALG+DGIVLSNHGGRQLDGALSTM
Sbjct: 261 LSAALDWADVQWLRGIWPGKLVLKGVMQVEDAQRAVALGVDGIVLSNHGGRQLDGALSTM 320

Query: 301 DVLPEVVAAVKGQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLAAGGQAGAARA 360
           DVLPEVVAAVKGQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLAAGGQAGA RA
Sbjct: 321 DVLPEVVAAVKGQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLAAGGQAGATRA 380

Query: 361 LDILRSEVDRVLALLACPDIDQLDATYLRRL 391
           L+ILRSEVDRVLALLACPDIDQLDA+YLRRL
Sbjct: 381 LEILRSEVDRVLALLACPDIDQLDASYLRRL 411


>gi|300311906|ref|YP_003775998.1| L-lactate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 380

 Score =  256 bits (653), Expect = 3e-69,   Method: Compositional matrix adjust.
 Identities = 142/373 (38%), Positives = 210/373 (56%), Gaps = 18/373 (4%)

Query: 17  IEELRAIARKRVPNFCFEYVEGGAEEEISLRRNREVFSHIGFLPRTLVDVSVRRQARKLF 76
           +E+ R +A+KRVP   ++Y + G+  E + R N +  + +    R  ++V  R     + 
Sbjct: 7   VEDFRLLAKKRVPKAFYDYADSGSYTESTYRANSKDLAALKLRQRVAINVDERSTRTTMI 66

Query: 77  GQEIASPFLIGPTGFSGLLAREGDIAMASAAAGAGIPFVLTNVSTTSLEEVVRRSGAQVW 136
           G ++  P  I PTG +G+    G++  A AA   GIPF L+ +S  S+E+V   +    W
Sbjct: 67  GHDVTMPVAIAPTGLTGMQWANGEMLGAIAAEKFGIPFTLSTMSICSIEDVASVTTKPFW 126

Query: 137 QQVYLYRDRAFVASVAQRAQAAGIDTLVLTTDSAVYGKREWDVRN-FSSPRRLDWRNKLD 195
            Q+Y+ RDR FV S+ +RA+AA    LVLT D  + G+R  D++N  S P +L     LD
Sbjct: 127 FQLYVMRDRGFVKSLIERAKAAKCSALVLTLDLQILGQRHKDLKNGMSVPPKLTLETLLD 186

Query: 196 VLCHPRWLIDILYPHGFPRFANLGDLLPADQTSVRGAAASILGQSLS--------PALDW 247
           +   P W +  L   G   F NL          ++G   +   Q+LS        P L+W
Sbjct: 187 LASKPGWALRAL--GGRKTFGNLAG-------HIKGGEGAGGVQTLSKWTASQFDPTLNW 237

Query: 248 RDVQWLRGIWNGKLVLKGVMQVQDAQRAVALGMDGIVLSNHGGRQLDGALSTMDVLPEVV 307
            DV W++  W GKL+LKG++ V+DA+ AV  G D IV+SNHGGRQLDGA+S+++ LP + 
Sbjct: 238 DDVAWIKQQWGGKLILKGILDVEDAKLAVQSGADAIVVSNHGGRQLDGAMSSIEALPAIA 297

Query: 308 AAVKGQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLAAGGQAGAARALDILRSE 367
            AV  Q+ V  DGG R G D++KA+ALGA   ++GRA  Y L A G  G ++ L+I+R E
Sbjct: 298 QAVGDQIEVWFDGGIRSGQDVLKAVALGARGTMIGRAFLYSLGAMGGEGVSQMLEIMRKE 357

Query: 368 VDRVLALLACPDI 380
           +D  +AL    DI
Sbjct: 358 LDVSMALTGTKDI 370


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.323    0.138    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,189,124
Number of extensions: 49287
Number of successful extensions: 219
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 215
Number of HSP's successfully gapped: 2
Length of query: 391
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 304
Effective length of database: 1,207,925
Effective search space: 367209200
Effective search space used: 367209200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 131 (55.1 bits)