BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0212.p (601 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309541|ref|YP_003773633.1| aspartyl-tRNA synthetase [Herba... 1232 0.0 gi|300312097|ref|YP_003776189.1| lysyl-tRNA synthetase [Herbaspi... 75 1e-14 >gi|300309541|ref|YP_003773633.1| aspartyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 599 Score = 1232 bits (3187), Expect = 0.0, Method: Compositional matrix adjust. Identities = 594/599 (99%), Positives = 598/599 (99%) Query: 3 MRTQYCGLTTEALLGQTVSLCGWVHRRRDHGGVIFIDLRDREGLVQVVCDPDRADVFKNA 62 MRTQYCGLTTEALLGQTVSLCGWVHRRRDHGGVIFIDLRDREGLVQVVCDPDRADVFKNA Sbjct: 1 MRTQYCGLTTEALLGQTVSLCGWVHRRRDHGGVIFIDLRDREGLVQVVCDPDRADVFKNA 60 Query: 63 ESVRNEFCLRITGLVRNRPEGTTNANLKSGKIEVLCHELEVLNPSVTPPFQLDDDSLSET 122 ESVRNEFCLRITGLVRNRPEGTTNANLKSGKIEVLCHELEVLNPSVTPPFQLDDDSLSET Sbjct: 61 ESVRNEFCLRITGLVRNRPEGTTNANLKSGKIEVLCHELEVLNPSVTPPFQLDDDSLSET 120 Query: 123 TRLTHRVLDLRRPQMQNNLRLRYKVAMEVRKFLDAQGFIDIETPMLTKSTPEGARDYLVP 182 TRLTHRVLDLRRPQMQNNLRLRYKVAMEVRKFLDAQGFIDIETPMLTKSTPEGARDYLVP Sbjct: 121 TRLTHRVLDLRRPQMQNNLRLRYKVAMEVRKFLDAQGFIDIETPMLTKSTPEGARDYLVP 180 Query: 183 SRVNAGEFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETSFL 242 SRVNAGEFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETSFL Sbjct: 181 SRVNAGEFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETSFL 240 Query: 243 NEQEIRDLFEGMIRLVFKNALDIDLPNPFPVMDFATAMGKYGSDKPDMRVKLEFTELTDV 302 NEQEIRDLFEGMIRLVFKNALDIDLPNPFPVMDFATAMGKYGSDKPDMRVKLEFTELTDV Sbjct: 241 NEQEIRDLFEGMIRLVFKNALDIDLPNPFPVMDFATAMGKYGSDKPDMRVKLEFTELTDV 300 Query: 303 MKDVDFKVFSGAANSEGGRVVGLRVPGGAEMPRSEIDAYTQFVAIYGAKGLAYIKVNEKA 362 MKDV+FKVFSGAANSEGGRVVGLRVPGGAEMPRSEIDAYTQFVAIYGAKGLAYIKVNEKA Sbjct: 301 MKDVEFKVFSGAANSEGGRVVGLRVPGGAEMPRSEIDAYTQFVAIYGAKGLAYIKVNEKA 360 Query: 363 KGRDGLQSPIVKNIHDAALNAIIEKTGAQDGDLIFFGADKAKVVNDAIGALRVKIGHSEF 422 KGRDGLQSPIVKNIHDAALNAI+EKTGAQDGDLIFFGADKAKVVNDAIGALRVKIGHSEF Sbjct: 361 KGRDGLQSPIVKNIHDAALNAILEKTGAQDGDLIFFGADKAKVVNDAIGALRVKIGHSEF 420 Query: 423 GKKNGLFDDEWRPLWVVDFPMFEYDEDGQRWVALHHPFTSPKDGHEDFISTNPGAAIAKA 482 GKKNGLFDD WRPLWVVDFPMFEYDEDGQRWVALHHPFTSPKDGHEDFISTNPGAAIAKA Sbjct: 421 GKKNGLFDDAWRPLWVVDFPMFEYDEDGQRWVALHHPFTSPKDGHEDFISTNPGAAIAKA 480 Query: 483 YDMVLNGWELGGGSVRIHRADVQSKVFRALKIDDEEARLKFGFLLDALQYGAPPHGGIAF 542 YDMVLNGWELGGGSVRIHRADVQSKVFRALKIDDEEA+LKFGFLLDALQYGAPPHGGIAF Sbjct: 481 YDMVLNGWELGGGSVRIHRADVQSKVFRALKIDDEEAKLKFGFLLDALQYGAPPHGGIAF 540 Query: 543 GLDRLVTMMAGAESIRDVIAFPKTQRAQCLLTHAPSEVDEKQLRELHIRLRNTEPAVKA 601 GLDRLVTMMAGAESIRDVIAFPKTQRAQCLLTHAPSEVDEKQLRELHIRLRNTEPAVK+ Sbjct: 541 GLDRLVTMMAGAESIRDVIAFPKTQRAQCLLTHAPSEVDEKQLRELHIRLRNTEPAVKS 599 >gi|300312097|ref|YP_003776189.1| lysyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 513 Score = 75.5 bits (184), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 76/325 (23%), Positives = 141/325 (43%), Gaps = 38/325 (11%) Query: 3 MRTQYCGLTTEALLGQ--TVSLCGWVHRRRDHGGVIFIDLRDREGL-----VQVVCDPDR 55 + +Y G EAL + TV++ G + +R G F L+D G+ +Q+ D Sbjct: 49 LHAKYDGFENEALEAEPVTVTVAGRMMLKRVMGKAAFATLQDASGVRADGRIQLYITRD- 107 Query: 56 ADVFKNAESVRNEFCLRITGLVRNRPEGTTNANLKSGKIEVLCHELEVLNPSVTPPFQLD 115 E F G + EGT K+G++ V +L+++ S+ P Sbjct: 108 ----VTGEEAMAAFKHYDLGDILG-AEGTLFKT-KTGELSVKVTKLKLITKSLRPLPDKF 161 Query: 116 DDSLSETTRLTHRVLDL-RRPQMQNNLRLRYKVAMEVRKFLDAQGFIDIETPMLTKSTPE 174 + + R +DL + + + R +R+F++ GF+++ETPML P Sbjct: 162 HGLADQEMKYRQRYVDLIMSEETRRTFKARTAAMSSIRRFMETHGFMEVETPML-HPIPG 220 Query: 175 GARDYLVPSRVNAGEF-FALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFT 233 GA + NA + + +P+L+ + L+V F+R ++I + FR+E + PEFT Sbjct: 221 GAAAKPFITHHNALDMQMFMRIAPELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFT 280 Query: 234 QIDCETSFLNEQEIRDLFEGMIR----------LVFKNALDIDLPNPFPVMDFATAMGKY 283 ++ ++++ Q + E +IR ++ ++DL PF + A+ KY Sbjct: 281 MMEFYAAYVDYQWLMGFTEQVIRQAAIDAHGTAVLSYQGRELDLSKPFDRLTITGAILKY 340 Query: 284 GSDKPDMRVKLEFTELTDVMKDVDF 308 + +TE + D+DF Sbjct: 341 APN---------YTEAQ--LADIDF 354 Score = 64.3 bits (155), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 58/212 (27%), Positives = 90/212 (42%), Gaps = 50/212 (23%) Query: 375 NIHDAALNAIIEKTGAQDGDLIF-------FG--ADKAKVVNDAIGALRVKIGHSEFGKK 425 I A L T AQ D+ F FG D+A + IGAL++ Sbjct: 332 TITGAILKYAPNYTEAQLADIDFLRTELKKFGVKTDQAPLAGAGIGALQL---------- 381 Query: 426 NGLFDDE-----WRPLWVVDFPMFEYDEDGQRWVALHHPFTSPKDGHEDFISTNPGAAIA 480 LF++ W P +++D+P+ SP D + A I Sbjct: 382 -ALFEETAESQLWNPTYIIDYPVE----------------VSPLARASDTV-----AGIT 419 Query: 481 KAYDMVLNGWELGGGSVRIHRADVQSKVFRAL----KIDDEEARLKFGFLLDALQYGAPP 536 + +++ + G E+ G ++ A+ Q+ F A DEEA + AL+YG PP Sbjct: 420 ERFELFITGREIANGFSELNDAEDQAARFHAQVAAKDAGDEEAMYYDADYIRALEYGMPP 479 Query: 537 HGGIAFGLDRLVTMMAGAESIRDVIAFPKTQR 568 GG G+DRL+ ++ + +IRDVI FP +R Sbjct: 480 AGGCGIGIDRLMMLITDSPNIRDVILFPHLRR 511 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,837,233 Number of extensions: 77373 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 176 Number of HSP's successfully gapped: 3 Length of query: 601 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 510 Effective length of database: 1,188,985 Effective search space: 606382350 Effective search space used: 606382350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)