BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0203.p (155 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309530|ref|YP_003773622.1| general secretion pathway prote... 256 7e-70 >gi|300309530|ref|YP_003773622.1| general secretion pathway protein [Herbaspirillum seropedicae SmR1] Length = 183 Score = 256 bits (653), Expect = 7e-70, Method: Compositional matrix adjust. Identities = 125/155 (80%), Positives = 135/155 (87%) Query: 1 MRRSLLPSRRHPAFLFLQRGLARLRWILILTVLAVAAALLVPPLVARFNHDHSPQALAAE 60 MRRSL PSRRHPAFLFLQRGLARLRWIL+LTVLAV AALLVPPLV RFNHDHSPQA AAE Sbjct: 29 MRRSLQPSRRHPAFLFLQRGLARLRWILVLTVLAVGAALLVPPLVTRFNHDHSPQAQAAE 88 Query: 61 KDMQAIAAGLERYRQDNGRYPSAAQGLLALVIKPTRAPVPGNWQIGGYVDRLPRDPWGNS 120 KDM+AIAAGLERYRQDNGRYPS QGLLALVI+P R P+ WQ+GGYV+RLPRDPWGN Sbjct: 89 KDMKAIAAGLERYRQDNGRYPSTQQGLLALVIRPVREPLANGWQLGGYVERLPRDPWGNP 148 Query: 121 YQYGADDDGTSYELYSFGQAGPDGDPDGEDVLRQH 155 YQY ADDDG SYEL+SFGQAGP+G D + V+ H Sbjct: 149 YQYQADDDGASYELFSFGQAGPEGGDDDDRVISLH 183 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.140 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 558,454 Number of extensions: 24027 Number of successful extensions: 70 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 70 Number of HSP's successfully gapped: 1 Length of query: 155 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 77 Effective length of database: 1,250,540 Effective search space: 96291580 Effective search space used: 96291580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)