BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0157.p (403 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 806 0.0 gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 557 e-160 gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 514 e-147 gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 354 9e-99 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 101 1e-22 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 806 bits (2081), Expect = 0.0, Method: Compositional matrix adjust. Identities = 393/404 (97%), Positives = 398/404 (98%), Gaps = 1/404 (0%) Query: 1 MTAFIRRAALSA-ACALLGAATLPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMA 59 MT FIRRAALSA ACALLGAA LPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMA Sbjct: 1 MTPFIRRAALSATACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMA 60 Query: 60 IADAGGTINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAG 119 IADAGG INGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAG Sbjct: 61 IADAGGAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAG 120 Query: 120 EKKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAF 179 EKKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAF Sbjct: 121 EKKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAF 180 Query: 180 GTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAI 239 GTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAI Sbjct: 181 GTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAI 240 Query: 240 KAANEFGVNKTMKLAGLLIFINDTHSLGLQMTQGMYLTDGWYWDLNDQSRAWANRYFLKM 299 KAANEFGVNKTMKLAGLLIFINDTHSLGL +TQGMYLTDGWYWDLNDQ+RAWANRYF+KM Sbjct: 241 KAANEFGVNKTMKLAGLLIFINDTHSLGLSLTQGMYLTDGWYWDLNDQTRAWANRYFMKM 300 Query: 300 KKMPSMFQAGDASAVGQYLKAVKAVGSTDADQVMDYLRKHRIEDFFTSNGVVRPDGRMVH 359 KKMPSMFQAGDASAVGQYLKAVKAVGSTDAD+VMDYLRK+RI DFFTSNGVVRPDGRMVH Sbjct: 301 KKMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVRPDGRMVH 360 Query: 360 DMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 403 DMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK Sbjct: 361 DMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 404 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 557 bits (1435), Expect = e-160, Method: Compositional matrix adjust. Identities = 260/378 (68%), Positives = 309/378 (81%) Query: 26 QAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAIADAGGTINGKKVELVFADHQNKADI 85 Q +GD IKIGFITD+SG Y+D DGQGG EAI+MAIAD GG INGKK+EL+ ADHQNKAD+ Sbjct: 25 QISGDVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAINGKKIELLVADHQNKADV 84 Query: 86 AANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKVFISVGAGTTRQTNEECNAYTI 145 AA+KAREWFD+ GVDML+GG NS +LA KVA EKKKV++ +GAG+ R TNEEC+ YT+ Sbjct: 85 AASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTV 144 Query: 146 QYAYDTTALARGTGAAITRQGGKSWYFLTADYAFGTSLEKDTSEVVKSNGGNVVGATRVP 205 YAYDT ALA+GTG+A+ +QGGKSW+FLTADYAFG SLE DT+ VVK++GG VVGA + P Sbjct: 145 HYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHP 204 Query: 206 LATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAANEFGVNKTMKLAGLLIFINDTHS 265 L SDFSSFLLQAQSSKAQI+GLA AGGD IN++KAANEFG+ KT KLAGLL+FIND HS Sbjct: 205 LGASDFSSFLLQAQSSKAQIIGLANAGGDFINSVKAANEFGITKTAKLAGLLVFINDVHS 264 Query: 266 LGLQMTQGMYLTDGWYWDLNDQSRAWANRYFLKMKKMPSMFQAGDASAVGQYLKAVKAVG 325 L L+ T+G+YLTD WYWD N+++RA+A RYF KKMPS QA D SA QYLK VK +G Sbjct: 265 LTLKNTEGLYLTDSWYWDANEETRAFAKRYFAVRKKMPSSLQAADYSAALQYLKVVKEIG 324 Query: 326 STDADQVMDYLRKHRIEDFFTSNGVVRPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTI 385 + D+D+VM LRK + D + NG +R DGR++HDMYL +VK SESK PWDYYKLVQTI Sbjct: 325 TDDSDKVMAALRKAKFNDMYQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYKLVQTI 384 Query: 386 PGEQAYMTKAESKCALWK 403 P EQA+ TKAESKCA WK Sbjct: 385 PAEQAWTTKAESKCAAWK 402 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 514 bits (1324), Expect = e-147, Method: Compositional matrix adjust. Identities = 248/402 (61%), Positives = 312/402 (77%), Gaps = 8/402 (1%) Query: 5 IRRAALSAACALLG---AATLPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAIA 61 +RR L+AACA+ G A L AQ + D ++IGFITDMSG YAD DG GG+EAIRMA+A Sbjct: 7 LRR--LAAACAIAGIFSGAAL--AQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRMAVA 62 Query: 62 DAGGTINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEK 121 D GGT+ GK +E++ ADHQNKADIAA +AREW D G+DML+GGVNSA +L+M KV EK Sbjct: 63 DFGGTVLGKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMAEK 122 Query: 122 KKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAFGT 181 K+V+I++GAGT R TNEEC YT+ Y YDT ALA+GT +A+ +QGGKSW+FL ADYAFG Sbjct: 123 KRVYINIGAGTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADYAFGH 182 Query: 182 SLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKA 241 SL DTS VVK++GG VVG+ + P +SD SSFLLQAQ+SKAQILGLA AG D +N+IKA Sbjct: 183 SLANDTSAVVKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQILGLANAGEDTVNSIKA 242 Query: 242 ANEFGVNKTMKLAGLLIFINDTHSLGLQMTQGMYLTDGWYWDLNDQSRAWANRYFLKMKK 301 A EFGV KTMKL GLL+ IND H+LGL +G+ +TD WYWD ++ SR +A R+F KMKK Sbjct: 243 AKEFGVTKTMKLVGLLMVINDIHALGLANAEGLMMTDSWYWDKDEPSRKFAERFFQKMKK 302 Query: 302 MPSMFQAGDASAVGQYLKAVKAVGSTDADQVMDYLRKHRIEDFFTSNGVVRPDGRMVHDM 361 MPS QA SA YLKAV+A+G+ D+ +VM L+K RI+DF+ + G +R DGR +HDM Sbjct: 303 MPSTHQAAAYSATTTYLKAVQAIGTDDSTKVMAQLKKTRIDDFY-NKGSIRADGRNIHDM 361 Query: 362 YLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 403 YL +VK P+ESK+PWDY KL++TIPGEQA+ + A+SKC+L K Sbjct: 362 YLYQVKSPAESKKPWDYLKLLETIPGEQAFTSVADSKCSLLK 403 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 354 bits (908), Expect = 9e-99, Method: Compositional matrix adjust. Identities = 187/399 (46%), Positives = 258/399 (64%), Gaps = 5/399 (1%) Query: 9 ALSAACAL-LGAATLPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAIADA-GGT 66 A + AC + L A +P+ +T+KIG +TDMSG Y+ GQG V A +MA+ D Sbjct: 12 AAAVACTVSLAATAIPAWAQDANTVKIGVLTDMSGPYSAMGGQGSVVAAKMAVEDCLKNE 71 Query: 67 INGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKVFI 126 G K+E++ AD+QNK D+ +AREW DRD V+ + NS+ +LA+ K+ +K + + Sbjct: 72 CKGMKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVALAVQKLIKDKGGIAM 131 Query: 127 SVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAFGTSLEKD 186 G T T EC + +DT + A G AA+T+ G KSWYF+T DYAFG SLEKD Sbjct: 132 YSGPATGALTTAECAPNGFHWMFDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHSLEKD 191 Query: 187 TSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAANEFG 246 S+VVK+NGG VVG+ R PL SDFSSFLLQAQ+SKAQ++GLA G DV+NAIK A F Sbjct: 192 ASDVVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQARSFA 251 Query: 247 V-NKTMKLAGLLIFINDTHSLGLQMTQGMYLTDGWYWDLNDQSRAWANRYFLKMKKM-PS 304 V K +L LL+F++D H+LGL+ QG+ TDG+YWD ++Q+RA++ R+ + K + P+ Sbjct: 252 VGGKDQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAFSKRFESQFKNIKPT 311 Query: 305 MFQAGDASAVGQYLKAVKAVGSTDADQVMDYLRKHRIEDFFTSNGVVRPDGRMVHDMYLM 364 M QAG S+V Q+LKA A ++D V +R+ I D N +RPDGR++HDMYL Sbjct: 312 MVQAGVYSSVYQFLKARAAAKTSDWKVVAQKMREIPIADAVMRNASIRPDGRVIHDMYLY 371 Query: 365 EVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 403 +VK P+ESK PWDY KL+ IP +QA+ ++ C L K Sbjct: 372 QVKTPAESKGPWDYLKLLSVIPAQQAF-RPMDAACPLVK 409 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 107/410 (26%), Positives = 180/410 (43%), Gaps = 31/410 (7%) Query: 4 FIRRAALSAACALLGAATLPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAIADA 63 F++ +AL+A GA + A ++I G MSG +A +G+ +++A+ Sbjct: 7 FLKLSALAAP----GALSYHDVFAQAESIAFGCPVPMSGPFAA-NGKFADLGMKLALQKY 61 Query: 64 GGTINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKK 123 G + GK V D + K A K +E ++ G GG+ S +LAMGK A + Sbjct: 62 GKVL-GKSTSYVNLDTEGKPATAVRKMQESIEK-GTRFFAGGILSGEALAMGKEADKAGA 119 Query: 124 VFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQ--GGKSWYFLTADYAFGT 181 VFI+ AG T +CN T +++ T T + + K WY +T Y FG Sbjct: 120 VFITT-AGADEITGSDCNRATFRWSVPTFGAIEQTVRPLLEKMPNAKRWYTITPQYVFGE 178 Query: 182 SLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKA 241 L + K G VG + L +FS +L A ++K +L L G + ++ Sbjct: 179 GLLSAAKNIFKEKGIEHVGNSYHSLTEKEFSGYLTNAIAAKPDVLLLLNFGSQSSDTLRQ 238 Query: 242 ANEFGV--NKTMKLA---GLLIFINDTHSLGLQMTQGMYLTDGWYWDLNDQSRAWANRYF 296 A FG+ N T+ +A GL F +LG + G+Y YW D A N+ F Sbjct: 239 AVSFGMKNNCTILMAWASGLEQF----ETLGADICDGVYF-GAQYWHAID---APFNKEF 290 Query: 297 LK-----MKKMPSMFQAGDASAVGQYLKAVKAVGSTDADQVMDYLRKHRIEDFFTSNGVV 351 +K +K P+ AG L A+ STD V+ + + E S + Sbjct: 291 VKLVNDNLKIDPNYSLAGSYICTKIILDAIIKANSTDPKAVIAAMEGMKYEGLTGSEEIR 350 Query: 352 RPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCAL 401 + D +++ + YL++ K ++ DY +V + ++++ +++C L Sbjct: 351 KGDHQVIKNYYLLKGKAKAKMANKNDYADIVSS---GKSFLPLEKTQCKL 397 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,179,915 Number of extensions: 49240 Number of successful extensions: 201 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 11 Length of query: 403 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 316 Effective length of database: 1,207,925 Effective search space: 381704300 Effective search space used: 381704300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)