BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0156.p (529 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309489|ref|YP_003773581.1| NAD-dependent dehydrogenase [He... 1004 0.0 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 74 2e-14 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 67 3e-12 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 63 8e-11 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 59 1e-09 >gi|300309489|ref|YP_003773581.1| NAD-dependent dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 529 Score = 1004 bits (2597), Expect = 0.0, Method: Compositional matrix adjust. Identities = 508/529 (96%), Positives = 520/529 (98%) Query: 1 VSDKILGHNYIGGQRSGKGDIALHSVDATTGALFETPFLTATEHEVAAAVDAAEKAYPLY 60 +S ILGHNYIGGQRSG+GD+ALHSVDATTGALFETPFLTAT+ EVAAAV AAE+AYPLY Sbjct: 1 MSFNILGHNYIGGQRSGQGDVALHSVDATTGALFETPFLTATDKEVAAAVHAAEQAYPLY 60 Query: 61 RAIAAEQRALFLEAIADEIDALGDDFLAAVSRETALPATPRLAGERARTSGQMRLFAKVV 120 RA +EQRA FLEAIADEIDALGDDFLAAV+RETALPATPRLAGERARTSGQMRLFAKVV Sbjct: 61 RATTSEQRAQFLEAIADEIDALGDDFLAAVARETALPATPRLAGERARTSGQMRLFAKVV 120 Query: 121 RRGDFYGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGASNFPLAFSVAGGDTAAALAA 180 RRGDFYGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGASNFPLAFSVAGGDTAAALAA Sbjct: 121 RRGDFYGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGASNFPLAFSVAGGDTAAALAA 180 Query: 181 GCPVVFKAHSGHLVTSELVANAIERAVKKTGMPAGTFNMIYGDRVGAQLVKSAGIQAVGF 240 GCPVVFKAHSGHLVTSELVA+AIERAVKKTGMPAGTFNMIYGDRVGAQLVKSAGIQAVGF Sbjct: 181 GCPVVFKAHSGHLVTSELVADAIERAVKKTGMPAGTFNMIYGDRVGAQLVKSAGIQAVGF 240 Query: 241 TGSLRGGRALCDMAAARPQPIPVFAEMSSINPIILMSEALKVRGDAIAKDLAGSVTVGVG 300 TGSLRGGRALCDMAAARPQPIPVFAEMSSINPIILM EALK+RGDAIAKDLAGSVTVGVG Sbjct: 241 TGSLRGGRALCDMAAARPQPIPVFAEMSSINPIILMPEALKLRGDAIAKDLAGSVTVGVG 300 Query: 301 QLCTSPGLLLGVRSPELTAFIEKLSAAFGGSNPATMLNSGGLTHYNGGVARLTQLPGVKV 360 QLCTSPGLLLGVRSPELT+FIEKLSAAFGG+NPATMLNSGGLTHYNGGVARLTQLPGVKV Sbjct: 301 QLCTSPGLLLGVRSPELTSFIEKLSAAFGGTNPATMLNSGGLTHYNGGVARLTQLPGVKV 360 Query: 361 IATGGTSYTQAVPHLFKADAALLFSKEAPLEEEVFGPSTVIVELESREQLLDFAAKMNGQ 420 IATGGTSYTQAVPHLFKADAALLFSKEAPLEEEVFGPSTVIVELESREQLLDFAAKMNGQ Sbjct: 361 IATGGTSYTQAVPHLFKADAALLFSKEAPLEEEVFGPSTVIVELESREQLLDFAAKMNGQ 420 Query: 421 LTATLQAEIGDLQGNQDLIAILEQKAGRLLLNGFPTGVEVCDAMVHGGPYPATSDARGTS 480 LTATLQAEIGDLQGNQDLIAILEQKAGRLLLNGFPTGVEVCDAMVHGGPYPATSDARGTS Sbjct: 421 LTATLQAEIGDLQGNQDLIAILEQKAGRLLLNGFPTGVEVCDAMVHGGPYPATSDARGTS 480 Query: 481 VGTLAIERFLRPVCYQNYPDAMLPAPLQNANPLGLMRLVDGEQTRATVG 529 VG+LAIERFLRPVCYQNYPDAMLPA LQNANPLGLMRLVDGEQTRATVG Sbjct: 481 VGSLAIERFLRPVCYQNYPDAMLPAALQNANPLGLMRLVDGEQTRATVG 529 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 74.3 bits (181), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 81/282 (28%), Positives = 127/282 (45%), Gaps = 31/282 (10%) Query: 49 AVDAAEKAYPLYRAIAAEQRALFLEAIADEIDALGDDFLAAVSRETALPATPRLAGERAR 108 A++AA K + +R ++A +R+ + AD + ++ ++ E P LA + Sbjct: 47 ALEAASKGFETWRNMSAFERSKIMRRAADLLRERAEEVARLMTMEQGKP----LAEAKME 102 Query: 109 TSG---QMRLFAKVVRRGDFYGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGASNFPL 165 T G + FA+ RR YG + +P + K VGPVA F NFPL Sbjct: 103 TLGAADTIDWFAEEARRT--YGRLVPARVPGAYQM------VIKEPVGPVAAFTPWNFPL 154 Query: 166 AFSVAGGDTAAALAAGCPVVFKAHSGHLVT-SELVANAIERAVKKTGMPAGTFNMIYG-- 222 V +AALAAGC ++ KA + +EL+ RA G+PAG N++YG Sbjct: 155 NQVVR--KLSAALAAGCSIIVKAPEETPASPAELI-----RAYADAGVPAGVINLVYGVP 207 Query: 223 DRVGAQLVKSAGIQAVGFTGSLRGGRALCDMAAARPQPIPVFAEMSSINPIILMSEALKV 282 + L+ I+ + FTGS G+ L +A + + E+ P ++ +A Sbjct: 208 AEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRATM--ELGGHAPAMVFDDA--- 262 Query: 283 RGDAIAKDLAGSVTVGVGQLCTSPGLLLGVRSPELTAFIEKL 324 D AK LA + GQ+C SP L V+ AF++K Sbjct: 263 DVDVAAKMLATAKFRNAGQVCVSPTRFL-VQKGVFNAFVDKF 303 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 67.0 bits (162), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 123/476 (25%), Positives = 185/476 (38%), Gaps = 67/476 (14%) Query: 9 NYIGGQRSG--KGDIALHSVDATTGALFETPFLTATEHEVAAAVDAAEKAYPLYRAIAAE 66 N+IGG+ KG+ ++ G F ++ +V A+DAA A + + Sbjct: 21 NFIGGKFVPPVKGEY-FENISPVIGRAF-CEVARSSAEDVELALDAAHAAKKSWGKTSPT 78 Query: 67 QRALFLEAIADEIDALGDDFLAAVSRETALPATPRLAGERARTSGQMRLFAKVVRRGDFY 126 +RA L IAD ++A + A + + P +A + R FA VR + Sbjct: 79 ERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGS 138 Query: 127 GARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGA---SNFPLAFSVAGGDTAAALAAGCP 183 ID D Y P+ V G NFP+ +V A ALAAG Sbjct: 139 ICPIDN-----------DTYAYHFH-EPLGVVGQIIPWNFPILMAV--WKLAPALAAGNC 184 Query: 184 VVFK----AHSGHLVTSELVANAIERAVKKTGMPAGTFNMI--YGDRVGAQLVKSAGIQA 237 VV K + +V EL+A+ I P G N++ +G G L + I Sbjct: 185 VVLKPAEQTPASIMVLIELIADLI---------PPGVVNIVQGFGVEAGKPLASNKRIAK 235 Query: 238 VGFTGSLRGGRALCDMAAARPQPIPVFAEMSSINPIILMSEALKVRGDAIAKDLAGSVTV 297 + FTG GR + M A IPV E+ +P I ++ L D K L G Sbjct: 236 IAFTGETTTGRLI--MQYASQNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMF 293 Query: 298 GV--GQLCTSPGLLLGVRSPELTAFIE---KLSAAFGGSNP---ATMLNSGGLTHYNGGV 349 + G++CT P +L V+ FIE K AA NP +TM+ + + Sbjct: 294 ALNQGEVCTCPSRVL-VQESIYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKI 352 Query: 350 ARLTQL---PGVKVIATGG--------TSYTQAVPHLFKADAALLFSKEAPLEEEVFGPS 398 + G KV+A GG S P +F+ + +K +EE+FGP Sbjct: 353 LSYIDIGKQEGAKVLAGGGREELGGDLASGYYVKPTVFQGN-----NKMRIFQEEIFGPV 407 Query: 399 TVIVELESREQLLDFAAKMNGQLTATLQAEIGDLQGNQDLIAILEQKAGRLLLNGF 454 + + E+ L A N L L A + G + E +AGR+ N + Sbjct: 408 VSVTTFKDEEEALAIA---NDTLYG-LGAGLWTRDGTRAFRMGREIQAGRVWTNCY 459 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 62.8 bits (151), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 77/286 (26%), Positives = 122/286 (42%), Gaps = 25/286 (8%) Query: 45 EVAAAVDAAEKAYPLYRAIAAEQRALFLEAIADEIDALGDDFLAAVSRETALP-ATPRLA 103 E A++AA A+P ++ A++R+ L + + A DD ++ E P A R Sbjct: 50 ETRRAIEAANAAWPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEAR-- 107 Query: 104 GERARTSGQMRLFAKVVRRGDFYGARIDTALPQRQPLPRPDIRQYKIGVGPVAVFGASNF 163 GE + + FA+ +R YG I + P P I K +G A NF Sbjct: 108 GEIGYAASFIEWFAEEGKRT--YGDTIPS------PSPSNRIVVIKEAIGVCAAITPWNF 159 Query: 164 PLAFSVAGGDTAAALAAGCPVVFKAHSGHLVTSELVANAIERAVKKTGMPAGTFNMIYG- 222 P A ALAAGCP+V K ++ +A ERA G+PAG F+++ G Sbjct: 160 PAAMITR--KAGPALAAGCPMVLKPAEATPFSALALAVLAERA----GIPAGVFSVVTGT 213 Query: 223 -DRVGAQLVKSAGIQAVGFTGSLRGGRALCDMAAARPQPIPVFAEMSSINPIILMSEALK 281 +G ++ + ++ + FTGS G+ L +A+ + + + E+ P I+ +A Sbjct: 214 PKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSL--ELGGNAPFIVFDDA-- 269 Query: 282 VRGDAIAKDLAGSVTVGVGQLCTSPGLLLGVRSPELTAFIEKLSAA 327 DA + S GQ C + V+ AF KL A Sbjct: 270 -DLDAAVEGAIASKYRNAGQTCVCANRIY-VQDGVYDAFAAKLVEA 313 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 58.9 bits (141), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 84/306 (27%), Positives = 131/306 (42%), Gaps = 35/306 (11%) Query: 28 ATTGALFETPFLTATEHEVAAAVDAAEKAYPLYRAIAAEQRALFLEAIADEIDALGDDFL 87 AT L P+ A ++ A+ AA +A+ +R ++ +R+ L A Sbjct: 28 ATGDVLGRLPW--AERADIEEAIQAAHRAFQQWRQVSPLERSDILRRAAALARERAQAMA 85 Query: 88 AAVSRETALPATPRLAGERARTSGQMRLFAKVVRR--GDFYGARIDTALPQRQPLPRPDI 145 A++ + P +A E + + A+ RR G GAR P++ Sbjct: 86 PAITMDNGKPLADAVA-EIINAAEHLDWHAEEGRRLYGRVVGAR------------HPEV 132 Query: 146 RQYKIG--VGPVAVFGASNFPLAFSVAGGDTAAALAAGCPVVFKAHSGHLVTSELVANAI 203 RQ + VG A NFP F A AAA+AAGC VV K S L+A Sbjct: 133 RQLVVREPVGVCAAISPWNFP--FGQAMKKVAAAMAAGCTVVLKGPSESPAAIVLMAQLF 190 Query: 204 ERAVKKTGMPAGTFNMIYG--DRVGAQLVKSAGIQAVGFTGSLRGGRALCDMAAARPQPI 261 A G+PAG FN++ G + L++S ++ + FTGS+ G L AA + + Sbjct: 191 HDA----GVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMKRM 246 Query: 262 PVFAEMSSINPIILMSEALKVRGDAIAKDLAGSVTVGVGQLCTSPGLLL---GVRSPELT 318 + E+ P+++ +A + A+ LA T GQ+C SP G+ + +T Sbjct: 247 SM--ELGGHAPVLVFDDA---EVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFVT 301 Query: 319 AFIEKL 324 AF E L Sbjct: 302 AFAEAL 307 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,659,550 Number of extensions: 72514 Number of successful extensions: 286 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 284 Number of HSP's successfully gapped: 9 Length of query: 529 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 439 Effective length of database: 1,193,720 Effective search space: 524043080 Effective search space used: 524043080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)