BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.0099.p
         (432 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309432|ref|YP_003773524.1| glutamate-cysteine ligase [Herb...   851   0.0  

>gi|300309432|ref|YP_003773524.1| glutamate-cysteine ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 433

 Score =  851 bits (2199), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 411/433 (94%), Positives = 422/433 (97%), Gaps = 1/433 (0%)

Query: 1   MVPHLVTALNGPLLELEKKILGATPAIERWFRMEWQEHTPPFYCSVDLRNAGFKLAPVDT 60
           MVPHLVTALNGPLLELEKKILGATPAIERWFRMEWQEHTPPFYCSVDLRNAGFKLAPVDT
Sbjct: 1   MVPHLVTALNGPLLELEKKILGATPAIERWFRMEWQEHTPPFYCSVDLRNAGFKLAPVDT 60

Query: 61  NLFPGGFNNLSPEMLPLAVQAAMAAIEKYCPDARNLLLIPENQPGNTFYLQNIGRLMQIF 120
           NLFPGGFNNLSPEMLPLAVQAAMAAIEKYCPDARNLLLIPEN  GNTFYLQNI RLMQIF
Sbjct: 61  NLFPGGFNNLSPEMLPLAVQAAMAAIEKYCPDARNLLLIPENHTGNTFYLQNIARLMQIF 120

Query: 121 RQTGLNVRLGTLAPGATKPQQIELPDGTHITLEPLERSANGRRLGLKNFDPCTVLLNNDL 180
           RQTGLNVRLG+LAPG  KP+ IELPDGT ITLEPLERSANGRRLGLKNFDPCTVLLNNDL
Sbjct: 121 RQTGLNVRLGSLAPGVNKPKPIELPDGTTITLEPLERSANGRRLGLKNFDPCTVLLNNDL 180

Query: 181 SSGVPSVLEGLYEQSVLPPLHAGWALRRKSNHFAAYDEVVKKFSKMVEVDPWMLNPLFTK 240
           S G+PSVLEGL+EQSVLPPLHAGWA+RRKSNHFAAYDEVVKKF+KMVEVDPWMLNPLF K
Sbjct: 181 SIGIPSVLEGLHEQSVLPPLHAGWAMRRKSNHFAAYDEVVKKFAKMVEVDPWMLNPLFAK 240

Query: 241 VSNIDFQDNTGTDALADSVSSLLSKIRKKYKEYGIKEQPFVFIKANAGTYGMGIMTVKDA 300
           VS+IDFQ++TGTDALADSVSSLLSKIRKKYKEYGIKEQPFVF+KANAGTYGMGIMTVKDA
Sbjct: 241 VSDIDFQESTGTDALADSVSSLLSKIRKKYKEYGIKEQPFVFVKANAGTYGMGIMTVKDA 300

Query: 301 SEIKDLNRKQRSGML-AGKEGLEVSEVIVQEGVHSFERINQAVAEPVVYMIDRYVVGGFY 359
           SEIKDLNRKQR+GML AGKEGLEVSEVIVQEGVHSFERINQAVAEPVVYMIDRYVVGGFY
Sbjct: 301 SEIKDLNRKQRTGMLAAGKEGLEVSEVIVQEGVHSFERINQAVAEPVVYMIDRYVVGGFY 360

Query: 360 RVHADRGVNENLNAPGAHFVPLAFAQQHALPDMHAKPGTAAPNRFYMYGVVARLALLAAS 419
           RVHADR VNENLNAPGAHFVPLAFAQQHALPDMHAKPGTAAPNRFYMYGVVARLALLAAS
Sbjct: 361 RVHADRDVNENLNAPGAHFVPLAFAQQHALPDMHAKPGTAAPNRFYMYGVVARLALLAAS 420

Query: 420 VEMEKTDPNPEVY 432
           VEMEKTDPNPEVY
Sbjct: 421 VEMEKTDPNPEVY 433


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,368,683
Number of extensions: 56745
Number of successful extensions: 145
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 144
Number of HSP's successfully gapped: 1
Length of query: 432
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 344
Effective length of database: 1,203,190
Effective search space: 413897360
Effective search space used: 413897360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 132 (55.5 bits)