BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0098.p (511 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309431|ref|YP_003773523.1| ammonium transporter transmembr... 876 0.0 gi|300309914|ref|YP_003774006.1| ammonium transporter transmembr... 117 2e-27 >gi|300309431|ref|YP_003773523.1| ammonium transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 876 bits (2264), Expect = 0.0, Method: Compositional matrix adjust. Identities = 490/511 (95%), Positives = 497/511 (97%), Gaps = 1/511 (0%) Query: 1 MSMKKMFSGVLAIGALLLAVGFTAPAQAQDAPKPEAAAAAAAAPAAAAPAAPKADAAAPA 60 MSMKKMFSG LAIGALL AV FTAPAQAQDAPKPEAAAAAAAAPAAA A AAAPA Sbjct: 1 MSMKKMFSGALAIGALLFAVSFTAPAQAQDAPKPEAAAAAAAAPAAAPAAPKAE-AAAPA 59 Query: 61 AAAPAAPAAAAAAPAPVPNKGDTAWMLISTAFVILMTLPGLGLFYGGLVRSKNMLSVLMQ 120 AAAPAAPAAAAAAPAPVPNKGDTAWML+ TAFVILMTLPGLGLFYGGLVRSKNMLSVLMQ Sbjct: 60 AAAPAAPAAAAAAPAPVPNKGDTAWMLVCTAFVILMTLPGLGLFYGGLVRSKNMLSVLMQ 119 Query: 121 CFVIFSLIYILWFVYGYSIAFTEGNAFFGGFDRLFLHGLTGDSNTGTFSKGVVIPELSFV 180 CF+IFSLIYILWFVYGYSIAFTEGNAFFGGFDRLFLHGLTGDSNTGTFSKGVVIPEL FV Sbjct: 120 CFMIFSLIYILWFVYGYSIAFTEGNAFFGGFDRLFLHGLTGDSNTGTFSKGVVIPELVFV 179 Query: 181 AFQGAFAAITVALIIGSFAERVKFSAVILFSILWFTFSYLPMAHMVWFWPGPDAFTDAAA 240 AFQGAFAAITVALIIG+FAERVKFSAVILFSILWFTFSYLPMAHMVWFWPGPDAFTDAAA Sbjct: 180 AFQGAFAAITVALIIGAFAERVKFSAVILFSILWFTFSYLPMAHMVWFWPGPDAFTDAAA 239 Query: 241 AEKATAISGWLFQKGALDFAGGTVVHINAAIAGLVGSYVIGKRIGFGKEAFKPHSLTLTM 300 EKATAISGWLFQKGALD+AGGTVVHINAAIAGLVGSYVIGKRIGFGKEAFKPHSLTLTM Sbjct: 240 GEKATAISGWLFQKGALDYAGGTVVHINAAIAGLVGSYVIGKRIGFGKEAFKPHSLTLTM 299 Query: 301 VGASLLWFGWFGFNAGSALEANGSAALAFVNTILATAAAVLSWSFGEWIGKGKPSMLGAA 360 VGASLLWFGWFGFNAGS LEANG+AALAFVNTILATAAAVLSWSFGEWIGKGKPSMLGAA Sbjct: 300 VGASLLWFGWFGFNAGSGLEANGTAALAFVNTILATAAAVLSWSFGEWIGKGKPSMLGAA 359 Query: 361 SGAVAGLVAITPAAGFVGVIGAVVMGLIAGLLCLWGVSGLKKILGSDDALDVFGVHGVGG 420 SGAVAGLVAITPAAGFVGVIGAVVMGLIAGLLCLWGVSGLKKILGSDDALDVFGVHGVGG Sbjct: 360 SGAVAGLVAITPAAGFVGVIGAVVMGLIAGLLCLWGVSGLKKILGSDDALDVFGVHGVGG 419 Query: 421 ILGALLTGVFAAPSLGGTGIYDYVANAVAPDYSIGGQVWIQFQGVLTTVILSGVVSFIAY 480 ILGA+LTGVFAAPSLGGTGIYDYVANAVAPDYSIGGQVWIQFQGVLTTV+LSGVVSFIAY Sbjct: 420 ILGAILTGVFAAPSLGGTGIYDYVANAVAPDYSIGGQVWIQFQGVLTTVVLSGVVSFIAY 479 Query: 481 KIVDVVIGLRVTEEEEREGLDISSHGESAYH 511 KIVDVVIGLRVTEEEEREGLDISSHGESAYH Sbjct: 480 KIVDVVIGLRVTEEEEREGLDISSHGESAYH 510 >gi|300309914|ref|YP_003774006.1| ammonium transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 117 bits (293), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 119/439 (27%), Positives = 194/439 (44%), Gaps = 59/439 (13%) Query: 79 NKGDTAWMLISTAFVILMTLPGLGLFYGGLVRSKNMLSVLMQCFVIFSLIYILWFVYGYS 138 G A ++ A +IL G G VR KN ++ L++ F++ I++F GY Sbjct: 6 KNGADALFILLGAIMILAMHAGFAFLELGTVRKKNQVNALVKILADFAVSTIVYFFIGYY 65 Query: 139 IAFTEGNAFFGGFDRLFLHGLTGDSNTGTFSKGVVIPELSFVAFQGAFAAITVALIIGSF 198 +A+ +FF G + L + F EL F FAA A+I G Sbjct: 66 VAYRV--SFFAGAETL--------AQRSGF-------ELVKFFFLLTFAAAIPAIISGGI 108 Query: 199 AERVKFSAVILFSILWFTFSYLPMAHMVWFWPGPDAFTDAAAAEKATAISGWL---FQKG 255 AER KF+ ++ + + Y F + A + + WL F Sbjct: 109 AERSKFNPQLVATAVLVGLVY--------------PFFEGIAWNQHLGVQAWLAATFDAE 154 Query: 256 ALDFAGGTVVHINAAIAGLVGSYVIGKRIG-FGKE----AFKPHSLTLTMVGASLLWFGW 310 DFAG VVH L ++G R G + KE A P ++ +GA +L GW Sbjct: 155 FHDFAGSIVVHAVGGWIALPAVLLLGARRGRYSKEGAVAAHPPSNIPFLALGAWILTVGW 214 Query: 311 FGFNAGSALEANGSAALAFVNTILATAAAVLSWSFGEWIGKGKPSMLGAASGAVAGLVAI 370 FGFN SA + L +N+++A A L +G+ P A +G +AGLVA+ Sbjct: 215 FGFNVMSAQTLDKMNGLVAMNSLMAMAGGTL---VALLMGRNDPGF--AYNGPLAGLVAV 269 Query: 371 TPAAGFVGVIGAVVMGLIAGLLCLWGVSGLKKILGSDDALDVFGVHGVGGILGALLTGVF 430 + + +GA+V G IAG + +W + + DD L V+ +HG+ G+ G L G+F Sbjct: 270 CAGSDLMHPLGALVTGGIAGAIFVWMFTRTQNKWKIDDVLGVWPLHGLCGLWGGLAAGIF 329 Query: 431 AAPSLGGTGIYDYVANAVAPDYSIGGQVWIQFQGVLTTVILSGVVSFIAYKIVDVVIGLR 490 +LGG G +++ Q G + ++++ + +I Y ++ +G+R Sbjct: 330 GLQALGGRGGVSFLS---------------QLIGSVMGIVIAALGGWIVYGLLKKAVGIR 374 Query: 491 VTEEEEREGLDISSHGESA 509 + E+E EG D++ H S+ Sbjct: 375 LDPEQEFEGADLAIHKISS 393 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,369,818 Number of extensions: 58237 Number of successful extensions: 223 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 216 Number of HSP's successfully gapped: 2 Length of query: 511 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 422 Effective length of database: 1,198,455 Effective search space: 505748010 Effective search space used: 505748010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)