BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0059.p (339 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 91 1e-19 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 88 8e-19 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 82 6e-17 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 59 7e-10 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 57 2e-09 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 90.9 bits (224), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 82/282 (29%), Positives = 129/282 (45%), Gaps = 23/282 (8%) Query: 5 MRRWRLHQFG-KQHLQLETLPIEQPAPGQILVRVRAVALNYRDKMNIDSGSGGPQVQLPF 63 MR + +FG + LQ P+ + G++L++V A +N D P Sbjct: 1 MRAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL 60 Query: 64 TPASDMAGEVVAVGAGVRCFAPGARVISTFWGGWLDGHWPPQAVALGAPGPGMLAQYVLL 123 P ++AGE+VA G F G V + GG A AP LAQ Sbjct: 61 -PGLEVAGEIVAGDLGDSGFKLGDLVCALVQGGGY-------AEFCAAP----LAQ---- 104 Query: 124 DEDWAVAAPLTLSDAQASTLPCAGLTAWTALIEQGQLHAGQTVVLQG-TGGVALFGLQLA 182 + P LS +A+ LP T W+ + ++GQL +T+++QG + G+ + +Q+A Sbjct: 105 ----CLPVPKGLSALEAAALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIA 160 Query: 183 AAQGARTIVISGDAGKLERALAMGATHGILRSLHPQWQHQVREMTQGRGADHVLELVGGE 242 A G R +G A K +GA I ++ V+E+T GRG D +L++VGG+ Sbjct: 161 RALGHRVFATAGSAEKCRACEELGAERAINYKTE-DFEAVVKELTGGRGVDVILDMVGGD 219 Query: 243 NVERSLQALTQGGRLSLIGLLGDMRLQASSIPLLLGRQSIQG 284 + R ++AL GRL I LG + Q ++ R +I G Sbjct: 220 YLPREIKALADDGRLVFIAQLGGAKGQLDMGQVMRRRLTITG 261 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 88.2 bits (217), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 91/309 (29%), Positives = 140/309 (45%), Gaps = 25/309 (8%) Query: 1 MDQTMRRWRLHQFGKQHLQLETLPIEQPAPGQILVRVRAVALNYRDKMNIDSGSGGPQVQ 60 M Q M+ + +FGK L +E +P+ PAPGQILV+ A + + D ++ G + Sbjct: 1 MTQMMKAAVVREFGKP-LSIEQVPVPTPAPGQILVKFEASGVCHTD-LHAAHGDWPVKPT 58 Query: 61 LPFTPASDMAGEVVAVGAGVRCFAPGARV-----------ISTFWGGWLDGHWPPQAVAL 109 PF P + G V AVGAGV+ G RV S GW Q Sbjct: 59 PPFIPGHEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNT-- 116 Query: 110 GAPGPGMLAQYVLLDEDWAVAAPLTLSDAQASTLPCAGLTAWTALIEQGQLHAGQTVVLQ 169 G G A+Y L D + P L A+ + CAG+T + L E ++ G+ VV+ Sbjct: 117 GYSVNGSFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKET-EVRPGEWVVIS 175 Query: 170 GTGGVALFGLQLAAAQGARTIVISGDAGKLERALAMGATHGILRSLHPQWQHQVREMTQG 229 G GG+ +Q A A G + KL A +GA + H + V E+ + Sbjct: 176 GIGGLGHMAVQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRNH----NAVAEVQRI 231 Query: 230 RGADH--VLELVGGENVERSLQALTQGGRLSLIGL-LGDMRLQASSIPLLLGRQSIQGIG 286 G H ++ V + +E++ L G ++L+GL GD+ L + +L R +++G Sbjct: 232 IGGAHGALVTAVSPKAMEQAFGFLRARGTMALVGLPPGDISLPVFNT--VLKRITVRGSI 289 Query: 287 VGHRRALED 295 VG R+ LE+ Sbjct: 290 VGTRQDLEE 298 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 82.0 bits (201), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 92/287 (32%), Positives = 122/287 (42%), Gaps = 50/287 (17%) Query: 4 TMRRWRLHQFG-KQHLQLETLPIEQPAPGQILVRVRAVALN-----YRDKMNIDSGSGGP 57 TM+ + H FG + L+ E P+ + PG++LVRV AV LN R+ + P Sbjct: 5 TMKAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQP 64 Query: 58 QVQLPFTPASDMAGEVVAVGAGVRCFAPGARVIST--FWGGWLDGHWPPQAVALGAPGPG 115 V P +D++G V AV V+ FA G V S F G A G Sbjct: 65 TVAFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGL-------------AGGSR 111 Query: 116 MLAQYVLLDEDWAVAAPLTLSDAQASTLPCAGLTAWTALIEQG-------QLH------- 161 AQYV + P +S AQA+ P + LTAW +IE G Q H Sbjct: 112 AYAQYVSVAASEIALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPL 171 Query: 162 AGQTVVLQGT-GGVALFGLQLAAAQGARTIVISGDAGKLERALAMGATHGILRSL-HPQW 219 +TVV+ G GGV F LQLA +GAR I A+ G LR L ++ Sbjct: 172 KDKTVVVNGAAGGVGHFALQLAKWKGARVI-----------AIGSGGHEAFLRDLGADEF 220 Query: 220 QHQVREMTQG--RGADHVLELVGGENVERSLQALTQGGRLSLIGLLG 264 R + D V++ VGG R L+ L +GG L I LG Sbjct: 221 IDYTRSAAEDVLSDVDLVIDAVGGPASGRFLRTLKRGGALFPIFPLG 267 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 58.5 bits (140), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 74/257 (28%), Positives = 111/257 (43%), Gaps = 30/257 (11%) Query: 5 MRRWRLHQFGKQH-LQLETLPIEQP-APGQILVRVRAVALNYRD-KMNIDSGSGGPQVQL 61 MR HQ+G L L L EQP PG + V ++A + D K+ + L Sbjct: 1 MRAICYHQYGPPSVLHLCDLD-EQPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDL 59 Query: 62 PFTPASDMAGEVVAVGAGVRCFAPGARVISTFWGGWLDGHWPPQAVALGAPGPGMLAQYV 121 P P D AG + A+G GV G V V G G + Sbjct: 60 PKVPGRDGAGVITALGEGVHTLQVGEAV----------------CVMPSMAGRGTTLAEL 103 Query: 122 LLDEDWAVAAPLTLSDAQASTLPCAGLTAWTALIEQGQLHAGQTVVLQ-GTGGVALFGLQ 180 +L ++ V P LS QA+ L +AW AL++ + AG V + G+G V +Q Sbjct: 104 VLPQEQVVRKPDVLSMQQAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQ 163 Query: 181 LAAAQGARTIVISGDAGKLERALAMGATHGILRSLHPQWQHQVREMTQGRGADHVLELVG 240 LAA GA + S + +E A+GA+ + + E+ + R D V++LVG Sbjct: 164 LAAHLGAE-VTASCRSSNVEYVAALGASRVL--------AYDRDELGRVRDQDVVIDLVG 214 Query: 241 GENVERSLQALTQGGRL 257 G ++S + L +GG+L Sbjct: 215 GAAHDQSYRMLRRGGQL 231 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 57.0 bits (136), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 66/249 (26%), Positives = 103/249 (41%), Gaps = 23/249 (9%) Query: 14 GKQHLQLETLPIEQPAPGQILVRVRAVALNYRDKMNIDSGSGGPQVQLPFTPASDMAGEV 73 G + ++ + + +P G++ +R AV LN+ D +G PQ LP + AG + Sbjct: 12 GPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFID-CYFRAGLY-PQ-PLPAGLGQEAAGTI 68 Query: 74 VAVGAGVRCFAPGARVISTFWGGWLDGHWPPQAVALGAPGPGMLAQYVLLDEDWAVAAPL 133 AVGAGV F G RV + G +G + + V + D V P Sbjct: 69 EAVGAGVSGFKVGDRVA---YAGRPNGAYAEERV---------------MPADILVKLPD 110 Query: 134 TLSDAQASTLPCAGLTAWTALIEQGQLHAGQTVVLQGT-GGVALFGLQLAAAQGARTIVI 192 +S A+ + G+T L + + G T++ GG+ L Q A A G I Sbjct: 111 AISFDTAAAMMLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGT 170 Query: 193 SGDAGKLERALAMGATHGILRSLHPQWQHQVREMTQGRGADHVLELVGGENVERSLQALT 252 K AL GA H ++ + +V+E+T GRG V + +G + SL L Sbjct: 171 VSSDEKAALALQHGAAH-VINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLR 229 Query: 253 QGGRLSLIG 261 G + G Sbjct: 230 PRGMMVSFG 238 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,110,084 Number of extensions: 46925 Number of successful extensions: 176 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 5 Length of query: 339 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 253 Effective length of database: 1,212,660 Effective search space: 306802980 Effective search space used: 306802980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)