BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0038.p (568 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 936 0.0 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 263 4e-71 gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 189 5e-49 gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 172 9e-44 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 155 8e-39 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 936 bits (2420), Expect = 0.0, Method: Compositional matrix adjust. Identities = 462/552 (83%), Positives = 493/552 (89%), Gaps = 6/552 (1%) Query: 16 PDIPPRPTITRAHYLQDGHGDQQERTPYAWHIPEFFNIAIAACDRWADGSGRTAILCEEN 75 P+I PR I+ AHY +PY W IP FNIA AACDRWADGSGRTAI+CE+ Sbjct: 3 PNIAPRSVISAAHY------RAGNPSPYEWQIPTHFNIAQAACDRWADGSGRTAIICEDA 56 Query: 76 DGSLTTWSFDQLKTLSDQFANALAEAGIGRGDRIGIYLSQRIETVITHLAAYKLGAVTVP 135 DGS+T++S+DQLK LSD+FANAL AG+GRGDRIGIYLSQRIETVITHLAAYKLGA+TVP Sbjct: 57 DGSVTSYSYDQLKILSDRFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVP 116 Query: 136 LFYLFGPQAIAYRLDNSEAVALVTDASGMDKLHLAGELPALKVVFCVEPSDIVLPQTTDF 195 LFYLFGP AIAYRLDNS AVALVTDASGM+K LAGELPAL++VFCVE SD+VLPQTTDF Sbjct: 117 LFYLFGPDAIAYRLDNSGAVALVTDASGMEKATLAGELPALRLVFCVESSDLVLPQTTDF 176 Query: 196 WDRLRAASPVLEPVMTRADDAAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQ 255 WDRL AA L+PV+T ADD AMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQ Sbjct: 177 WDRLHAAPAELDPVLTLADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQ 236 Query: 256 PGDRFWTPADWAWIGGLLDVLLPALYHGVAVLARRLEKFDAETVFGLLARHQIRNVFFPP 315 GDRFWTPADWAWIGGLLDVLLP+LYHGVAV+ARRLEKFDA VFGLLARHQIRNVFFPP Sbjct: 237 EGDRFWTPADWAWIGGLLDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFFPP 296 Query: 316 TALKMLRGTPDPRSRWPFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECNLVVS 375 TALKMLRG R++ FSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECNLVVS Sbjct: 297 TALKMLRGAATVRAQADFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECNLVVS 356 Query: 376 SSSQLYPSASGSMGRAVPGHEVQIVDEEGHLLPRGQVGHIAIRAPDPVMFLRYWRNEDAT 435 SSS YPS SGSMGRAVPGH VQIVDE+G +LP G VG+IAIRAPDPVMFLRYWRNE+AT Sbjct: 357 SSSHCYPSVSGSMGRAVPGHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFLRYWRNEEAT 416 Query: 436 REKFAGDFLLTGDLGNMDEAGYIRYLGRNDDVITSAGYRIGPAPIEECLMRHPAVRIAAV 495 REKFAGDFLLTGDLG+MDE GYIRYLGRNDDVITSAGYRIGPA IEECLMRHPAVRIAAV Sbjct: 417 REKFAGDFLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAAIEECLMRHPAVRIAAV 476 Query: 496 VGVKDALRTEVVKAFVVLKEGHAPGAALVAELQQHVRAQLAAHEYPRMIAFVDALPTTAT 555 VGVKDALRTEVVKAFVVLK+G P AL AELQQHVRAQLAAHEYPR+I+FV ALPTTAT Sbjct: 477 VGVKDALRTEVVKAFVVLKDGVTPDEALKAELQQHVRAQLAAHEYPRLISFVAALPTTAT 536 Query: 556 GKIMRKTLKEME 567 GKIMRKTLKEM+ Sbjct: 537 GKIMRKTLKEMD 548 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 263 bits (671), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 189/529 (35%), Positives = 271/529 (51%), Gaps = 29/529 (5%) Query: 51 FNIAIAACDRWADGSGRTAILCEENDGSLTTWSFDQLKTLSDQFANALAEAGIGRGDRIG 110 N + CDR G A+ G +SF Q+++++ + AN L + GI G + Sbjct: 37 INACVECCDRHVKGD-NIALEFVSLHGEHQQYSFAQVRSMAARVANLLRQQGIQPGQIVA 95 Query: 111 IYLSQRIETVITHLAAYKLGAVTVPLFYLFGPQAIAYRLDNSEAVALVTDASGMDKLHLA 170 L + E + T L + GAV PLF FGP+AI +RL S A +VT+ + DKL Sbjct: 96 GMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIVTNVANRDKLDEI 155 Query: 171 GELPALKVVFCVEPSDIVLPQTTDFWDRLRAASPVLEPVMTRADDAAMIIYTSGTTGKAK 230 P +V E +D + DF + A S PV+ D +++ TSGTTG AK Sbjct: 156 ANCP--QVCTVRESNDPLRAGDIDFRAAVDAQSDDFAPVLRSGADLMLMMSTSGTTGAAK 213 Query: 231 G------ALHAHRVLLGHLPGVEVSHDSFPQPGDRFWTPADWAWIGGLLDVLLP--ALYH 282 G AL A V + G+ P D+FW AD W GL ++ A+ H Sbjct: 214 GVPVPLSALQAFSVYMREAVGL--------LPQDKFWNMADPGWAYGLYYAIIGPLAIGH 265 Query: 283 GVAVLARRLEKFDAETVFGLLARHQIRNVFFPPTALKMLRGTPDPRS-RWPFSLRSVASG 341 G+ F + + R I ++ PTA +M+ + + LR+V+S Sbjct: 266 GITF---NEAAFTVSGTYETIRRLGITSLAGAPTAYRMMMAAGEEAAASVKGRLRAVSSA 322 Query: 342 GETLGDDLIAWGREALGVTINEFYGQTECNLVVSSSSQL-YPSASGSMGRAVPGHEVQIV 400 GE L +++ W AL V I++ YGQTE +VV++ L + GS G A+PG+ V ++ Sbjct: 323 GEPLNAEVVRWFASALDVPIHDHYGQTELGMVVNNHHALRHEVVPGSAGFAMPGYRVVVL 382 Query: 401 DEEGHLLPRGQVGHIAIR-APDPVM-FLRYWRNEDATREKFAGDFLLTGDLGNMDEAGYI 458 DE L Q G +AI A P+ F YW+ + A + TGD ++ G I Sbjct: 383 DENHQELGANQPGELAIDIARSPLYWFSGYWKQDTPA---IAHGYYSTGDNVELEPNGSI 439 Query: 459 RYLGRNDDVITSAGYRIGPAPIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKEGHA 518 ++GR+DDVITSAGYRIGP +E L+ HPAV AV+G+ D RTE+VKAFVVL + Sbjct: 440 SFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVIGLPDPERTEIVKAFVVLSDQFK 499 Query: 519 PGAALVAELQQHVRAQLAAHEYPRMIAFVDALPTTATGKIMRKTLKEME 567 AL +L QHV+ +L+AH YPR I F+DALP T +GK+ R L++ME Sbjct: 500 ASDALREQLAQHVKRRLSAHAYPRAIEFLDALPKTPSGKLQRFVLRKME 548 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 189 bits (480), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 169/569 (29%), Positives = 260/569 (45%), Gaps = 42/569 (7%) Query: 36 DQQERTPYAWHIPEFFNIAIAACD-RWADGSG-RTAILCEENDGSLTTWSFDQLKTLSDQ 93 D+ Y W N++ D +G+ +TA++ E +DG +T S+ +L Q Sbjct: 66 DESNAPFYKWFADGQVNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVSYRELHQKVCQ 125 Query: 94 FANALAEAGIGRGDRIGIYLSQRIETVITHLAAYKLGAVTVPLFYLFGPQAIAYRLDNSE 153 FAN L GI +GDR+ IY+ +E V A ++GA +F F +++ R+ ++ Sbjct: 126 FANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHERVIDAG 185 Query: 154 AVALVT---DASGMDKLHLA---------GELPALK--VVFCVEPSDIVLPQTTDFW--D 197 AVA++T G +L L G LK VV+ ++I + D W D Sbjct: 186 AVAVLTADYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEINWVEGRDLWLSD 245 Query: 198 RLRAASPVLEPVMTRADDAAMIIYTSGTTGKAKGALHAHR-VLLGHLPGVEVSHDSFPQP 256 + S EP A+ I+YTSG+TGK KG HA LL + ++ + D +P Sbjct: 246 VVANQSDTCEPEWVDAEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFDI--KP 303 Query: 257 GDRFWTPADWAWIGGLLDVLLPALYHGVA-VLARRLEKF-DAETVFGLLARHQIRNVFFP 314 D +W AD WI G + L G ++ + + +A + ++ARH+ + Sbjct: 304 DDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIFYTA 363 Query: 315 PTALKMLRGTPD------PRSRWPFSLRSVASGGETLGDDLIAWGREALG---VTINEFY 365 PTA++ L D P+ SLR + S GE + + W + +G I + + Sbjct: 364 PTAIRSLIKAADADEKVHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIVDTF 423 Query: 366 GQTEC-NLVVSSSSQLYPSASGSMGRAVPGHEVQIVDEEGHLLPRGQVGHIAIRAPDPVM 424 QTE ++S P GS +PG IVDE G+ +P G G + ++ P P M Sbjct: 424 WQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPWPSM 483 Query: 425 FLRYWRNEDATR-----EKFAGDFLLTGDLGNMD-EAGYIRYLGRNDDVITSAGYRIGPA 478 W + + + E+ G L GD + E GY GR DDV+ +G+R+G Sbjct: 484 IRTIWNDPERFKKSYFPEELGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTM 543 Query: 479 PIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKEGHAPG---AALVAELQQHVRAQL 535 IE L+ + V AAVVG D E + AFVVLK G + EL+ V ++ Sbjct: 544 EIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVAKEI 603 Query: 536 AAHEYPRMIAFVDALPTTATGKIMRKTLK 564 P+ I F D LP T +GKIMR+ L+ Sbjct: 604 GPIAKPKEIRFGDNLPKTRSGKIMRRLLR 632 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 172 bits (435), Expect = 9e-44, Method: Compositional matrix adjust. Identities = 165/575 (28%), Positives = 271/575 (47%), Gaps = 60/575 (10%) Query: 40 RTPYA-WHIPEFFNIAIAACDRW----ADGSGRTAILCEENDGSLTTWSFDQLKTLSDQF 94 R P+A W I N+ A DR A + AI E N + T++ Q + ++ Sbjct: 39 RPPFARWFIDGQTNLCHNAVDRHLPERAQQAALIAISTETNSERVYTYAELQQEVMA--M 96 Query: 95 ANALAEAGIGRGDRIGIYLSQRIETVITHLAAYKLGAVTVPLFYLFGPQAIAYRLDNSEA 154 A ++ G+ RGDR+ IY+ E V LA ++GAV +F F ++A R+D+++ Sbjct: 97 AASMQALGVQRGDRVLIYMPMIAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQP 156 Query: 155 VALVTDASGM-------------DKLHLAGELP--ALKVVFCVEPSDIVLPQTTDFWD-R 198 +V+ +G + + +A P L V + P + D+ R Sbjct: 157 RLIVSADAGSRGGKVIPYKGLLDEAIAMAQHRPQHVLLVDRGLAPMARTAQRDVDYAPLR 216 Query: 199 LRAASPVLEPVMTRADDAAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVS--HDSFPQP 256 + + ++ ++ I+YTSGTTGK KG R + G+ + S H QP Sbjct: 217 TQHLGQQVPVTWLESNASSYILYTSGTTGKPKGV---QRDVGGYAVALASSMQHIFCGQP 273 Query: 257 GDRFWTPADWAWIGGLLDVLLPALYHGVA-VLARRLE-KFDAETVFGLLARHQIRNVFFP 314 G+ ++ +D W+ G ++ L G+A VL L + DA + ++ ++++ +F Sbjct: 274 GETYFCTSDIGWVVGHSYIVYGPLIAGMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSA 333 Query: 315 PTALKMLRGTP-DPRSRW-PFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECN- 371 PTA+++L+ P + R+ SL+++ GE L + +W AL V + + Y QTE Sbjct: 334 PTAIRVLKKQPPECMERYDTSSLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGW 393 Query: 372 --LVVSSSSQLYPSASGSMGRAVPGHEVQIVDE-EGHLLPRGQVGHIAIRAPDPVMFLR- 427 + ++ P+ GS G +PG+ + I+DE G Q G +AI P P ++ Sbjct: 394 PIISIAKGIDDKPTRLGSPGVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQT 453 Query: 428 -----------YWRNEDATREKFAGDFLLTGDLGNMDEAGYIRYLGRNDDVITSAGYRIG 476 YWR+ RE ++ T D G D+ GY LGR DDVI AG+R+G Sbjct: 454 VYGDDERFVNTYWRS--LPREVYS-----TFDWGIRDKDGYYFILGRTDDVINVAGHRLG 506 Query: 477 PAPIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKEGHAPGA-----ALVAELQQHV 531 IEE + HP V AVVGV+D L+ +V AF + K+ + AL AE+ V Sbjct: 507 TREIEESISSHPNVSEVAVVGVEDKLKGQVAFAFAIPKQAYTIATPEQRKALEAEIMAVV 566 Query: 532 RAQLAAHEYPRMIAFVDALPTTATGKIMRKTLKEM 566 Q+ A P + FV LP T +GK++R+ ++ + Sbjct: 567 DKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQSI 601 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 155 bits (392), Expect = 8e-39, Method: Compositional matrix adjust. Identities = 137/508 (26%), Positives = 232/508 (45%), Gaps = 34/508 (6%) Query: 76 DGSLTTWSFDQLKTLSDQFANALAEAGIGRGDRIGIYLSQRIETVITHLAAYKLGAVTVP 135 DGS + ++ D A+AL G+ +GD++ ++ + + AA+K+G V VP Sbjct: 57 DGSDRLIPWREINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVP 116 Query: 136 LFYLFGPQAIAYRLDNSEAVALVTD---ASGMDKLHLAGELPALKVVFCVEPSDIVLPQT 192 + P AY +S A ++ D A +D + A PAL+ V + + Sbjct: 117 TNFRITPPEAAYLGQSSGACVMLYDRGFAQYVDAVKAAS--PALEHVVALADAR---AGE 171 Query: 193 TDFWDRLRAAS-PVLEPVMTRADDAAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHD 251 D+ +R S DD YTSGTTG K + +H G + V +H Sbjct: 172 LDYEALVREGSGAAFREAEVDYDDPLWFFYTSGTTGHPKAGMLSH----GQMAFVVTNHL 227 Query: 252 SFPQPG----DRFWTPADWAWIGGLLDVLLPALYHGVAVLARRLEKFDAETVFGLLARHQ 307 + PG R A + G+ V+ A G A + E+ E + L+ +++ Sbjct: 228 ADLMPGLTHQSRSLVVAPLSHGAGVHAVINTA--RGAASILLSSERLVVEEAWQLVEKYR 285 Query: 308 IRNVFFPPTALKMLRGTPDPRSRWPFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQ 367 + N+F PT +K+L SL+ V G + + + LG + ++YG Sbjct: 286 VDNLFTVPTIVKILVEDESVDRYDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYGL 345 Query: 368 TECNLVVSS-------SSQLYPSAS-GSMGRAVPGHEVQIVDEEGHLLPRGQVGHIAIRA 419 E ++ +P A G+ G G ++ I++++G L + G I +R Sbjct: 346 GEVTGNITFLPPYLHFEDDAHPQARVGTCGMPRTGMQIAILNDDGQELAPFETGEICVRG 405 Query: 420 PDPVMFLRYWRNEDATREKFAGDFLLTGDLGNMDEAGYIRYLGRNDDVITSAGYRIGPAP 479 P +F+ Y N +A + F D+ TGDLG++D+ G++ GR+ D+ S G + P Sbjct: 406 P--AVFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPRE 463 Query: 480 IEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKEG-HAPGAALVAELQQHVRAQLAAH 538 IEE L+ HPAV AV+GV D E A +V K G G AL+A H+ +++ + Sbjct: 464 IEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALLA----HLESRIGKY 519 Query: 539 EYPRMIAFVDALPTTATGKIMRKTLKEM 566 ++PR + D++P + GKI++K +K + Sbjct: 520 KWPRRFIYWDSMPKSGYGKIVKKQIKAL 547 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,934,983 Number of extensions: 88549 Number of successful extensions: 348 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 297 Number of HSP's successfully gapped: 17 Length of query: 568 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 478 Effective length of database: 1,193,720 Effective search space: 570598160 Effective search space used: 570598160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)