BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0035.p (462 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 736 0.0 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 268 7e-73 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 210 2e-55 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 146 3e-36 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 132 7e-32 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 736 bits (1901), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/454 (81%), Positives = 414/454 (91%) Query: 1 MSLRLVVLFGLLALALFTAVGSYLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLEDI 60 +SLRLV LFGLLA+ + A+G+YLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLEDI Sbjct: 9 LSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLEDI 68 Query: 61 LARRHLMLDTLSGHDQMLVRIARADGSTLMESHDPARSAPDLDLSAINVRSWDRIEALRV 120 LARRHL+LDTL+GHDQ+LVRIARADGSTL+ESHDP R+ P +DLS +++R+WD I++ ++ Sbjct: 69 LARRHLVLDTLTGHDQLLVRIARADGSTLLESHDPTRTPPGIDLSQVDIRAWDSIQSWKL 128 Query: 121 GELRARYIASMPATRSGEKTIVLVVRTASDRMLLLADYRADVWWAAGIGTLLATLLSYFL 180 G+LRARY+AS P TRSGE+ +VLVVRTASDRMLLLA+YR+DVWWAAG+G LLA L+SYFL Sbjct: 129 GDLRARYVASKPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGVGALLAILMSYFL 188 Query: 181 VRSGLSPLRMMAAQTRAISAHKLDTRLDLQAAPRELHEIVQSFNEMLDRLHRSFQQLSQF 240 VRSGL PLR MAAQTRA+SA++LDTRLDLQAAPRELHEIV+SFNEMLDRLHRSFQQLSQF Sbjct: 189 VRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHRSFQQLSQF 248 Query: 241 SADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMESMLFLARAD 300 SADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMESMLFLARA+ Sbjct: 249 SADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMESMLFLARAE 308 Query: 301 NAHVVLQLQELDVAEELSRIAEYFEGIAEEAQVRIAVSGAGRLRADTMLLRRAVGNLVAN 360 NAHVVLQ QELDVA+EL+RIA+YFEGIA+EAQVR+ V GAGRL+AD LLRRAVGNLVAN Sbjct: 309 NAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLLRRAVGNLVAN 368 Query: 361 AIRYTPADGVITLQASLTEGATIIEVANPGPGIPPDQVERIFDRFYRADPSRSSAGGSSG 420 AIRYTPADGVI LQ I V+NPG GIP +Q+ER+FDRFYRADPSRSSAGGSSG Sbjct: 369 AIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADPSRSSAGGSSG 428 Query: 421 LGLAIVRTIMALHGGQASVHSEPGRQTVFVLRFP 454 LGLAIVR+IM LHGGQASV S PG+ TVF LRFP Sbjct: 429 LGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRFP 462 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 268 bits (685), Expect = 7e-73, Method: Compositional matrix adjust. Identities = 179/469 (38%), Positives = 267/469 (56%), Gaps = 20/469 (4%) Query: 1 MSLRLVVLFGLLALALFTAVGSYLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLEDI 60 ++ R+ LF L+ + T +G YL S L +R D+ L G+ +L+ ++E Sbjct: 13 LTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFRNLMHDLYNVEQ- 71 Query: 61 LARRHLMLDTLSGHDQMLVRIARADGSTLMESHDPARSAPDL--------DLSAINVRSW 112 + +R + +++ G++Q VRI R G +P P +S+ ++R+ Sbjct: 72 MEQRPALFESMMGNEQD-VRIFRRQGEAPFIQSNPDHLVPPAMTPLPVGSPVSSASLRTS 130 Query: 113 DRIEALRVGELRARYIASMPATRSGEKTIVLV---VRTASDRMLLLADYRADVWWAAGIG 169 R + +RV R+++++ G T+ +V V RM+ YR + AA Sbjct: 131 QRADGVRV-----RWVSALADVGKGGDTVEIVAAYVMVQEARMM--RSYRWRIAGAAAAA 183 Query: 170 TLLATLLSYFLVRSGLSPLRMMAAQTRAISAHKLDTRLDLQAAPRELHEIVQSFNEMLDR 229 +L LL + L+R GL PL M+ + I+ L RLD + P EL + SFN MLDR Sbjct: 184 VVLTALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDR 243 Query: 230 LHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRM 289 L + L+QFS DLAH++RTP+N LM Q+QVALG+ R +EY+ +L SN+EE +RLSR+ Sbjct: 244 LEAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRI 303 Query: 290 MESMLFLARADNAHVVLQLQELDVAEELSRIAEYFEGIAEEAQVRIAVSGAGRLRADTML 349 +E++LFLA+AD+A + ++ LD+AEEL RI +YFEGIA+E +R V AG+ +A+ ++ Sbjct: 304 VENILFLAQADHAALAVERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQANLIM 363 Query: 350 LRRAVGNLVANAIRYTPADGVITLQASLTEGATIIEVANPGPGIPPDQVERIFDRFYRAD 409 RR V NLV NA+RY A+ I + A + I V N + + R+FDRFYR D Sbjct: 364 WRRVVSNLVINAVRYGRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGD 423 Query: 410 PSRSSAGGSSGLGLAIVRTIMALHGGQASVHSEPGRQTVFVLRFPTAQA 458 SRS+ S+GLGLAIV+ IMALHGG A+V F LRFP+A A Sbjct: 424 RSRSAFTESNGLGLAIVKAIMALHGGTAAVECPAPGWIRFSLRFPSAAA 472 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 210 bits (535), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 108/285 (37%), Positives = 178/285 (62%), Gaps = 4/285 (1%) Query: 168 IGTLLATLLSYFLVRSGLSPLRMMAAQTRAISAHKLDTRLDLQAAPRELHEIVQSFNEML 227 +G +LA + SY++ R+GL+PLR M+ + ++ S KL R+ ++A P+EL + +FN L Sbjct: 176 LGIVLAVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGAL 235 Query: 228 DRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLS 287 DR+ ++ QL F+AD+AH++RTPL NL+ +TQVAL + R E++ +L SN+EE +R+ Sbjct: 236 DRIEDAYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIR 295 Query: 288 RMMESMLFLARADNAHVVLQLQELDVAEELSRIAEYFEGIAEEAQVRIAVSGAGRLRA-- 345 ++ MLFLAR+D VA+E+ + ++FE + +++ +RI V+G A Sbjct: 296 SIVNDMLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMM 355 Query: 346 DTMLLRRAVGNLVANAIRYTPADGVITLQASLTEGATIIEVANPGPGIPPDQVERIFDRF 405 +T L +RA+ NL+ NAI+++P ++ + + E + V+NPG I + R+FDRF Sbjct: 356 ETALFKRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRF 415 Query: 406 YRADPSRSSAGGS--SGLGLAIVRTIMALHGGQASVHSEPGRQTV 448 YR D +R GS GLGLAIV+ + +HGG+A S+ G ++ Sbjct: 416 YRVDSARQDINGSHGHGLGLAIVKAVARMHGGRAFASSKEGVNSI 460 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 146 bits (369), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 99/293 (33%), Positives = 154/293 (52%), Gaps = 8/293 (2%) Query: 167 GIGTLLATLLSYFLVRSGLSPLRMMAAQTRAISAHKLDTRLDLQAAPRELHEIVQSFNEM 226 G T + + VR GL PL+ + KL +D + AP EL ++ + NE+ Sbjct: 168 GALTFASIFVVLVAVRRGLRPLKSLQRNLEQRDHGKLGA-IDGRGAPVELQPLIVAMNEL 226 Query: 227 LDRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERL 286 + R+++ Q F AD+AH +RTPL L +Q ++ K + + L+ ER+ Sbjct: 227 MARINKGEQAQQNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERM 286 Query: 287 SRMMESMLFLARADNA-HVVLQLQELDVAEELSRIAEYFEGIAEEAQVRIAVS-GAGRLR 344 R +L LAR++ +L+EL + + + ++F A++ ++ + RLR Sbjct: 287 IRQSRQLLALARSEPGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDLRPARLR 346 Query: 345 ADTMLLRRAVGNLVANAIRYTPADGVITLQASLTEGATIIEVANPGPGIPPDQVERIFDR 404 D LL + NL+ NA+RY+P G +T++ + AT++ V + GPGI P+ E IFDR Sbjct: 347 GDRFLLSDLIDNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDR 406 Query: 405 FYRADPSRSSAGGSSGLGLAIVRTIMALHGGQASVHS-EPGRQTVFVLRFPTA 456 FYRA+ + SGLGLAIVR I HGG SV E G+ T+F +RFP A Sbjct: 407 FYRANDKVA----GSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPLA 455 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 132 bits (331), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 93/272 (34%), Positives = 141/272 (51%), Gaps = 19/272 (6%) Query: 175 LLSYFLVRSGLSPLRMMAAQTRAISAHKLDTRLDLQAAPRELHEIVQSFNEMLDRLHRSF 234 +LS+ V GL+P+R AA+ A + L D+ P+E+ + + N++L RL + Sbjct: 155 VLSWVAVSRGLAPVRKAAAEVAARDMNSLQPLADV-GMPQEIQPLTHALNDLLARLKHAT 213 Query: 235 QQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQ--ALLSSNIEEYERLSRMMES 292 + F AD AH++RTPL L +QTQ+A R +DE + A + ER + ++ Sbjct: 214 ETQRAFVADAAHELRTPLTALRLQTQLA---ERAVDEEERRAAFADLKNGLERTTHLVHQ 270 Query: 293 MLFLARADNAHVVLQLQELDVAEELSRIAEYFEGIAEEAQVRIAVSGAG------RLRAD 346 +L LAR + + ++D+ L + +AE + + +S G R+ + Sbjct: 271 LLTLARQEPD--AMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGN 328 Query: 347 TMLLRRAVGNLVANAIRYTPADGVITLQASL-TEGATIIEVANPGPGIPPDQVERIFDRF 405 LR NLV NA+RYTPA GV+ + L T G + V + GPGI ++ R+FDRF Sbjct: 329 ADALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDRF 388 Query: 406 YRADPSRSSAGG----SSGLGLAIVRTIMALH 433 YRA S+ +G SGLGLAIVR I LH Sbjct: 389 YRAQHSQEGSGSRAAFGSGLGLAIVRQIADLH 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,232,405 Number of extensions: 47486 Number of successful extensions: 324 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 272 Number of HSP's successfully gapped: 40 Length of query: 462 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 373 Effective length of database: 1,198,455 Effective search space: 447023715 Effective search space used: 447023715 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)