BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0032.p (735 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309360|ref|YP_003773452.1| hypothetical protein Hsero_0014... 969 0.0 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 350 2e-97 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 269 7e-73 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 266 4e-72 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 260 3e-70 >gi|300309360|ref|YP_003773452.1| hypothetical protein Hsero_0014 [Herbaspirillum seropedicae SmR1] Length = 726 Score = 969 bits (2505), Expect = 0.0, Method: Compositional matrix adjust. Identities = 487/721 (67%), Positives = 584/721 (80%), Gaps = 1/721 (0%) Query: 12 LQHSLVLPVWSLDQAHMRALLQGAMLDSQVVAVEIRDPEEKIIIRQEEPLRRSGNLITRS 71 L+H LVLPVW+LDQ+ + ALL+GAMLD QVV++E+ DPEEK + R+ EP R +G+LI R+ Sbjct: 2 LRHGLVLPVWNLDQSDIMALLKGAMLDRQVVSIEVIDPEEKALARRAEPQRSAGHLIHRT 61 Query: 72 VPLVMPQQERKLSLGSVQVVMSDAQLQQKLAADKRFQTLVLLAQAVVSLLIIGLLVYRRI 131 PLVM Q ++ +SLGS+ +V+SD QLQ+KLAADK F LVLLAQA+VSLLIIGLLVYRRI Sbjct: 62 APLVMQQNQQDVSLGSLHIVVSDHQLQKKLAADKSFYALVLLAQAIVSLLIIGLLVYRRI 121 Query: 132 LAPISKLTQATERIQHGHFDTRIVLPQHDEIGVLACHMDAMQTSLKALFDEQAAILRNIP 191 L PI KLTQAT RIQ G F+ I LPQ DEIG LA HM+AMQ SLK LFDEQAAIL NIP Sbjct: 122 LGPIGKLTQATARIQRGSFEQGIDLPQQDEIGQLARHMEAMQASLKTLFDEQAAILGNIP 181 Query: 192 AGVLFVRDGRIAFANGAAQTMLGWPTDQLEALPCAQIFQDPLQQAVYVTNKLVREQGAAG 251 AGVLFVR G+I F N AAQ +L +L + +F + +QQAVYV + R GAAG Sbjct: 182 AGVLFVRKGKIVFGNEAAQALLARDDRRLVGVAAEDLFVESVQQAVYVARAVARSSGAAG 241 Query: 252 EIVLLKRGDGHSFPAELHCAMIDGASPGGQIWVIIDVSERLEAENRIDRLAFYDPVTELP 311 EIVLL+ G SFPAELH ++ID A PG QIWV+IDVS RLEAENRID+LAFYDPVT+LP Sbjct: 242 EIVLLRNASGASFPAELHTSLIDPARPGEQIWVVIDVSARLEAENRIDQLAFYDPVTQLP 301 Query: 312 NKKLFLDRLRRGLVRQQSQGSPIAVFYLEIHRADLSHGLVKADDADHLSKECARRLGACV 371 NKKLF+DRLRRGL R+++ G AVF +EIHRADLS+G VKAD+ + L ECARR +C+ Sbjct: 302 NKKLFMDRLRRGLDRRRTSGHAGAVFVMEIHRADLSYGSVKADEMEQLLIECARRFSSCI 361 Query: 372 SVDQTLARLDGEHFALGCEIRSEALADGIRFCEALARRLTAALAEPQGV-AVSFPYSLNI 430 + +QT+AR++GE FALG EIRS LA+ IR+CEALA+R+ A +PQ + A FP S+N+ Sbjct: 362 TAEQTVARMEGEKFALGTEIRSNELAECIRYCEALAQRILDAFTDPQSLSAARFPCSVNL 421 Query: 431 GINILRGQVQSAEEALMQAKLAMVQSLAAGRNAFRFFDPAMQALASQRSALEVELREAVA 490 GINI+R + A E +MQA+LAMVQS AAGRN FRFFDPAMQALA+QRS LE ELR+AVA Sbjct: 422 GINIVRDEQYGASELVMQAELAMVQSRAAGRNVFRFFDPAMQALANQRSQLEAELRQAVA 481 Query: 491 QGQFVVYYQPQVTYEGQVVGAEALVRWMHPSKGMVAPAQFIAVAEQLGLMEEIGHLVLEV 550 + QF+VYYQPQVTYEG VVGAEALVRW HP +G+V+P FI++AE +GLM+EIG VL Sbjct: 482 RRQFIVYYQPQVTYEGDVVGAEALVRWQHPIRGIVSPTTFISLAEDMGLMDEIGRQVLHR 541 Query: 551 ICCDLESWQRAGLAQDLVVAVNVSAQEFKVDHFVARFHETIRHHRLHSKAVKVELTESMM 610 IC DLE+WQR GLA+DLVVA+NVSAQEFKVD+FV RF E +R H + S +VKVELTESMM Sbjct: 542 ICTDLENWQREGLARDLVVAINVSAQEFKVDNFVYRFREIMREHHVPSHSVKVELTESMM 601 Query: 611 IENIDEVIEKMNDLKSHAVSISLDDFGTGYSSLSYLGRFPIDQIKIDQSFVRQIQTDAAT 670 +++I++VI KM L+S+ VSISLDDFGTGYSSLSYLGRFPIDQIKIDQ+FVR++Q DAA Sbjct: 602 VDHIEDVITKMQALRSNEVSISLDDFGTGYSSLSYLGRFPIDQIKIDQTFVREMQNDAAM 661 Query: 671 ASIIRSIIMLGQSLGLSIIAEGVETQEQRDLLHAQGCTLYQGFWYGRPMPGEQFAALLQT 730 ASIIRSIIMLGQSL LSIIAEGVET++QRD LHAQGC LYQG+WY +P+PGEQFA LL T Sbjct: 662 ASIIRSIIMLGQSLNLSIIAEGVETEQQRDFLHAQGCLLYQGYWYSKPIPGEQFADLLNT 721 Query: 731 P 731 P Sbjct: 722 P 722 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 350 bits (898), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 190/476 (39%), Positives = 280/476 (58%), Gaps = 3/476 (0%) Query: 257 KRGDGHSFPAELHCAMIDGASPGGQIWVII--DVSERLEAENRIDRLAFYDPVTELPNKK 314 +R +G +P L + + +V D+S+R AE RI LAF+D +T LPN+ Sbjct: 552 RRKNGEVYPEWLTISAVKNKDQQVTHYVSTHHDISDRKLAEERIRELAFFDALTRLPNRT 611 Query: 315 LFLDRLRRGLVRQQSQGSPIAVFYLEIHRADLSHGLVKADDADHLSKECARRLGACVSVD 374 L LDRL++ + + A+ ++++ + + + D L K+ A+RL A V + Sbjct: 612 LLLDRLKQAIALSLRTKTCAALLFIDLDHFKTLNDTIGHEKGDTLLKQVAQRLLANVREN 671 Query: 375 QTLARLDGEHFALGCEIRSEALADGIRFCEALARRLTAALAEP-QGVAVSFPYSLNIGIN 433 T+AR+ G+ F + E +E + A+ ++ AL Q V + + +IG Sbjct: 672 DTVARVGGDEFVVVLEGLNELQQEAATQTRAIGEKILTALGSTYQLDDVEYRTTASIGAT 731 Query: 434 ILRGQVQSAEEALMQAKLAMVQSLAAGRNAFRFFDPAMQALASQRSALEVELREAVAQGQ 493 + G+ S +E + QA LAM ++ GRNA RFFDPAMQ +R+ LE LR+A+ Q Sbjct: 732 VFLGRAASVDELMKQADLAMYKAKETGRNALRFFDPAMQTAVLERATLEAGLRKAIDGNQ 791 Query: 494 FVVYYQPQVTYEGQVVGAEALVRWMHPSKGMVAPAQFIAVAEQLGLMEEIGHLVLEVICC 553 +++YQPQV G+V GAE LVRW HP +GMV P FI +AE+ GL+ +G+ VLE C Sbjct: 792 LLLHYQPQVVEGGRVTGAEVLVRWQHPQRGMVPPGDFIPLAEETGLILSLGNWVLETACR 851 Query: 554 DLESWQRAGLAQDLVVAVNVSAQEFKVDHFVARFHETIRHHRLHSKAVKVELTESMMIEN 613 L +W DL +AVNVSA + + FV +TI + + +K+ELTES++++N Sbjct: 852 QLATWAAQPQMADLSIAVNVSAPQLREPDFVQTVLDTIERTGANPRRLKLELTESLLVDN 911 Query: 614 IDEVIEKMNDLKSHAVSISLDDFGTGYSSLSYLGRFPIDQIKIDQSFVRQIQTDAATASI 673 ++++I+KM LK++ V SLDDFGTGYSSLSYL R P++Q+KIDQSFVR + D ASI Sbjct: 912 VEDIIQKMFALKAYGVEFSLDDFGTGYSSLSYLKRLPLNQLKIDQSFVRDVLIDPNDASI 971 Query: 674 IRSIIMLGQSLGLSIIAEGVETQEQRDLLHAQGCTLYQGFWYGRPMPGEQFAALLQ 729 ++I+ L QSLGL +IAEGVET QR L GC YQG+++ RP+ E F Q Sbjct: 972 AKTIVNLAQSLGLGVIAEGVETDAQRSFLADAGCHAYQGYFFCRPVTVEAFEKFAQ 1027 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 269 bits (687), Expect = 7e-73, Method: Compositional matrix adjust. Identities = 158/446 (35%), Positives = 244/446 (54%), Gaps = 6/446 (1%) Query: 285 IIDVSERLEAENRIDRLAFYDPVTELPNKKLFLDRLRRGLVRQQSQGSPIAVFYLEIHRA 344 + D++ E ++ A D +T L N+ + L + + + Q + V ++++ R Sbjct: 502 LYDITSMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRF 561 Query: 345 DLSHGLVKADDADHLSKECARRLGACVSVDQTLARLDGEHFALGCEIRSEALADGIRFCE 404 + + D L + A RL A V T+ARL G+ F + + E D Sbjct: 562 KFVNDTLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILPEQDELTLD----TR 617 Query: 405 ALARRLTAALAEPQGVAVSFPYSLNIGINILRGQVQSAEEALMQAKLAMVQSLAAGRNAF 464 +L R + A A + + ++G+ E + A +AM + GRN F Sbjct: 618 SLQRIMDAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNF 677 Query: 465 RFFDPAMQALASQRSALEVELREAVAQGQFVVYYQPQVTY-EGQVVGAEALVRWMHPSKG 523 +F+ PAM A +R +E +LR A+ G+F+++YQP+V G+V+G+EAL+RW HP G Sbjct: 678 QFYTPAMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLG 737 Query: 524 MVAPAQFIAVAEQLGLMEEIGHLVLEVICCDLESWQRAGLAQDLVVAVNVSAQEFKVDHF 583 MV+PA+FI +AE+ GL+ IG VL C WQ G A D+ V+VN+S +F Sbjct: 738 MVSPARFIGLAEETGLILPIGAWVLRAACTQCRQWQLGGYA-DISVSVNLSVGQFLQKDL 796 Query: 584 VARFHETIRHHRLHSKAVKVELTESMMIENIDEVIEKMNDLKSHAVSISLDDFGTGYSSL 643 V+ + + L +++++ELTES+M+ +++ I + LK V +S+DDFGTGYSSL Sbjct: 797 VSSIADVLEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSL 856 Query: 644 SYLGRFPIDQIKIDQSFVRQIQTDAATASIIRSIIMLGQSLGLSIIAEGVETQEQRDLLH 703 +YL RFPID +KID+SFVR I DA A+I SII L + L L +IAEGVET EQ D L Sbjct: 857 AYLKRFPIDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLR 916 Query: 704 AQGCTLYQGFWYGRPMPGEQFAALLQ 729 GC QG+ + P+P ++F +L+ Sbjct: 917 HYGCDEIQGYLFSPPVPADKFEQILR 942 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 266 bits (681), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 161/445 (36%), Positives = 259/445 (58%), Gaps = 12/445 (2%) Query: 288 VSERLEAENRIDRLAFYDPVTELPNKKLFLDRLRRGLVRQQSQGSPIAVFYLEIHRADLS 347 ++ER E + A +D +T LPN+ + + L+R L + +G+ AV ++++ + Sbjct: 252 ITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAFVDLDQFKFI 311 Query: 348 HGLVKADDADHLSKECARRLGACVSVDQTLARLDGEHFALGCEIRSEALADGIRFCEALA 407 + + D L K A+RL AC+ +AR G+ F L + ++E A GI + Sbjct: 312 NDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEA-GI---AEVM 367 Query: 408 RRLTAALAEP-QGVAVSFPYSLNIGINILRGQVQSAEEALMQAKLAMVQSLAAGRNAFRF 466 +R+ AA+A P Q F + +IG++ + E L A AM ++ GRN F+F Sbjct: 368 QRILAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQF 427 Query: 467 FDPAMQALASQRSALEVELREAVAQGQFVVYYQPQVTY-EGQVVGAEALVRWMHPSKGMV 525 F P M + S R + + LR A+ Q +F +YYQP+++ +G+++GAEAL+RW P +GMV Sbjct: 428 FAPWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMV 487 Query: 526 APAQFIAVAEQLGLMEEIGHLVLEVICCDLESWQRAGLAQDLVVAVNVSAQEFK--VDHF 583 P +FI AE+ GL+ IG VL C ++WQ+AGL + VAVN+S ++ + F Sbjct: 488 PPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPP-IPVAVNLSPRQLNQSLPDF 546 Query: 584 VARFHETIRHHRLHSKAVKVELTESMMIENIDEVIEKMNDLKSHAVSISLDDFGTGYSSL 643 VA + L + +++E+TE++++++ ++ + ++ LK + IS+DDFGTGYSSL Sbjct: 547 VA---GVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSL 603 Query: 644 SYLGRFPIDQIKIDQSFVRQIQTDAATASIIRSIIMLGQSLGLSIIAEGVETQEQRDLLH 703 SYL RFP+D +KID+SFVR I DA +A+I+++II L L L +IAEGVE +EQ L Sbjct: 604 SYLRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLK 663 Query: 704 AQGCTLYQGFWYGRPMPGEQFAALL 728 C QG+++G+PM F A + Sbjct: 664 ENACDEVQGYYFGKPMEVAVFTAWM 688 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 260 bits (665), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 155/439 (35%), Positives = 245/439 (55%), Gaps = 12/439 (2%) Query: 294 AENRIDRLAFYDPVTELPNKKLFLDRLRRGLVRQQSQGSPIAVFYLEIHRADLSHGLVKA 353 A + ++ +D +T LPN+ L DRL + + + + A+ ++++ + + Sbjct: 258 ANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAINDSLGH 317 Query: 354 DDADHLSKECARRLGACVSVDQTLARLDGEHFALGCEIRSEALADGIRFCEALARRLTAA 413 D L E A+RL + + T+ARL G+ F + ++ + A G+ A +L A Sbjct: 318 HVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNTPEDAMGV------AEKLVEA 371 Query: 414 LAEPQGVAV-SFPYSLNIGINILRGQVQSAEEALMQAKLAMVQSLAAGRNAFRFFDPAMQ 472 + +P V S ++GI I + + ++ A AM + +GRN + FF+P+M Sbjct: 372 VNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMN 431 Query: 473 ALASQRSALEVELREAVAQGQFVVYYQPQVTY-EGQVVGAEALVRWMHPSKGMVAPAQFI 531 A A Q+ +LR A+ +GQF + YQP+ + G V+GAEAL+RW HP G+VAP QFI Sbjct: 432 ANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFI 491 Query: 532 AVAEQLGLMEEIGHLVLEVICCDLESWQRAGLAQDLVVAVNVSAQEFKVDHFVARFHETI 591 +AE+ GL+ IG VL+ C + W G D ++VN+SA +F H + T+ Sbjct: 492 GLAERSGLILPIGAWVLDEACRQMREWFALGYV-DWTMSVNLSALQFADPHLLEVVRTTL 550 Query: 592 RHHRLHSKAVKVELTESMMIENIDEVIEKMNDLKSHAVSISLDDFGTGYSSLSYLGRFPI 651 H+L + +E+TES + + + ++ + + V IS+DDFGTGYSSL YL R P Sbjct: 551 ERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPA 610 Query: 652 DQIKIDQSFVRQIQTDAATASIIRSIIMLGQSLGLSIIAEGVETQEQRDLLHAQGCTLYQ 711 +++KID+ FVR+++ D A+I+ +I+ LG++L L I+AEGVET EQ+D L A GC Q Sbjct: 611 NELKIDRGFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQ 670 Query: 712 GFWYGRPMPGEQFAALLQT 730 G+ GRPM E F LQT Sbjct: 671 GYLMGRPMTPELF---LQT 686 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,145,600 Number of extensions: 87520 Number of successful extensions: 355 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 311 Number of HSP's successfully gapped: 19 Length of query: 735 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 643 Effective length of database: 1,184,250 Effective search space: 761472750 Effective search space used: 761472750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)