BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0030.p (242 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310048|ref|YP_003774140.1| metal-dependent hydrolase [Herb... 97 9e-22 >gi|300310048|ref|YP_003774140.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] Length = 295 Score = 97.4 bits (241), Expect = 9e-22, Method: Compositional matrix adjust. Identities = 80/239 (33%), Positives = 119/239 (49%), Gaps = 12/239 (5%) Query: 3 KHSVIYGDQQIRFSLRYLQRPTRRVSIHVSPEGAVQVDAPEGTALPEIKQAVRKRAAWIW 62 K + G+ I F+L +R R + VS EG ++V AP+ L EI A+R++ WI Sbjct: 57 KRRIQLGEHTIDFALLRSKR--RTIGFLVSDEG-LRVTAPKWVTLAEIDNAIREKQRWIL 113 Query: 63 QQLQAVTRRRLHVL-PR-EYVSGETHFYLGRRHMLKVLRAEGGESGVRLWRGRLEIRAVR 120 +L R L P+ ++ G T YLG L++ G V L E+ Sbjct: 114 SKLNERRERSARRLQPQMQWRDGATLPYLGSDITLRI--RTGQRQAVYLDELTRELNISL 171 Query: 121 PDALTVRKL---LEDWYRQRATDVFAKVLLEMAPRLGLRRTMPPVRLLSMRTQWGSCSPK 177 P ++L ++ W + RA ++F K L+ A +LG++ L S TQWGSC+ Sbjct: 172 PADAGEQQLKDRVQGWIQARAEELFGKRLMIYAEKLGVQYRS--YALSSATTQWGSCTSD 229 Query: 178 GEILLNPSLVKAPRPCIEYVVAHELCHLREHNHSDRFYKHLARALPDWEARKAELDELA 236 I LN L+ P I+YV+AHEL HLRE NHS RF+ + P++E+ K L + A Sbjct: 230 ARIRLNWRLMHFALPLIDYVIAHELAHLREMNHSPRFWATVQSIFPEFESAKKALRDSA 288 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.137 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 737,721 Number of extensions: 30545 Number of successful extensions: 119 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 117 Number of HSP's successfully gapped: 1 Length of query: 242 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 159 Effective length of database: 1,226,865 Effective search space: 195071535 Effective search space used: 195071535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 129 (54.3 bits)