BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0050.0035.p (427 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA4343 [GEPR] COG0477 Permeases of the major facilitator superfa... 342 9e-94 RSc3059 [GEPR] COG0477 Permeases of the major facilitator superf... 311 2e-84 RSp0536 [GEPR] COG0477 Permeases of the major facilitator superf... 303 6e-82 RSc3060 [GEPR] COG0477 Permeases of the major facilitator superf... 296 5e-80 PA5476 [GEPR] COG0477 Permeases of the major facilitator superfa... 286 8e-77 >PA4343 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 439 Score = 342 bits (877), Expect = 9e-94, Method: Compositional matrix adjust. Identities = 181/409 (44%), Positives = 270/409 (66%), Gaps = 8/409 (1%) Query: 19 RAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRPLGAV 78 R + ++ IGNALEW+D ++YG + +IA+ FFP+GD+ SLL+ ATFGV FFMRP+G V Sbjct: 12 RQVAAAVIGNALEWYDFIVYGFLSSLIARLFFPSGDEYTSLLMALATFGVGFFMRPVGGV 71 Query: 79 VLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGE 138 +LG Y+DR GRKAA+ L I+LMT+ AMIAF P+YA+IG+ AP I + +M+QGF+ GGE Sbjct: 72 LLGLYADRRGRKAAMQLIILLMTLSIAMIAFAPTYAAIGVGAPLLIVIARMLQGFATGGE 131 Query: 139 FGSSTAFLVEHA-PHRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGWRVPF 197 + S+TAFLVE A PHRRG + SWQ+ Q +++ A GA++ + L E L SWGWRVPF Sbjct: 132 YASATAFLVESAPPHRRGLYGSWQLFGQCLAVFAGAGMGALVTHCLDAEALESWGWRVPF 191 Query: 198 IFGLLIAPAGIYIRRHLDEAPEF----KESSEKTNAPLRDTFAHQKMRLLIGAGSVIMAT 253 +FGLLI P G++IRR++ E F +E E+ L + +L+ G ++ T Sbjct: 192 LFGLLIGPVGLWIRRYMGETEAFLEAIREPGERQG--LLGVLREYRRSVLVSMGLTVVGT 249 Query: 254 VSVYLSLY-IPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSS 312 VS Y+ L +PT+A KQLGLP F A +M + PL G LSD++GR P ++ ++ Sbjct: 250 VSFYVVLVNMPTFAHKQLGLPLDEVFMVQMAAVALMTLVIPLAGGLSDRVGRRPVLLVAT 309 Query: 313 ALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSL 372 ++++ YP+F ++ +P +LLL+Q ++ + +F P +A+ FPV R TG+++ Sbjct: 310 LAFMLMVYPLFAWVAAAPSLGRLLLMQLLLCTAIGGFFGPAPTAVAEQFPVRVRSTGLAV 369 Query: 373 AYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSAR 421 AYN+AV +FGGFA I+TWL + G ++ +++V+ A L L+A+L R Sbjct: 370 AYNLAVMLFGGFAPFIVTWLTEVGGSPVAPAFYVLGAAFLGLLATLYLR 418 >RSc3059 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 431 Score = 311 bits (797), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 178/406 (43%), Positives = 247/406 (60%), Gaps = 9/406 (2%) Query: 19 RAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRPLGAV 78 R+I GN LE +D ++YG +A IA FFP ++ +SL+L+ TFGV F MRPLGA+ Sbjct: 12 RSIFRVVSGNFLEMYDFMVYGYYAKAIADTFFPADNEFLSLMLSLVTFGVGFLMRPLGAL 71 Query: 79 VLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGE 138 +LGAY DR GR+A L+L++ LM GT +IA +P YA+IGL AP + +G+++QGFSAG E Sbjct: 72 ILGAYIDRHGRRAGLILTLGLMACGTLLIALVPGYATIGLAAPLLVLIGRLLQGFSAGVE 131 Query: 139 FGSSTAFLVE-HAPHRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGWRVPF 197 G + +L E P R+GFF SWQ ASQ ++++ AAL G +L+ L+P+++ WGWRVPF Sbjct: 132 LGGVSVYLAEISTPGRKGFFVSWQSASQQVAVMFAALLGVLLSFQLSPKEMGEWGWRVPF 191 Query: 198 IFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAH--QKMRLLIGAGS--VIMAT 253 + G LI P IRR L E EFK+ K L + F + R L+GAG+ V+M T Sbjct: 192 LVGCLIVPFLFLIRRSLQETEEFKQ--RKHRPALGEIFRSMGENWR-LVGAGTLLVVMTT 248 Query: 254 VSVYL-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSS 312 VS YL + Y PT+ L L S G F P++G++SD+IGR P +IT + Sbjct: 249 VSFYLITAYTPTFGKSVLHLSNLDSLIVTLCVGASNFFWLPVMGSVSDRIGRRPLLITFT 308 Query: 313 ALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSL 372 AL ++ YP +L P F +LL ++ + L Y AM L +I P A R TG SL Sbjct: 309 ALTLLTAYPAMQWLIAEPSFGRLLAVELWLSFLYGSYNGAMVVTLTEIMPPAVRTTGFSL 368 Query: 373 AYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASL 418 AY++A IFGGF I TWLI TG+K ++ F A+ L+A+L Sbjct: 369 AYSLATAIFGGFTPAIATWLIHATGNKAMPGLWLSFAAICGLVATL 414 >RSp0536 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 441 Score = 303 bits (775), Expect = 6e-82, Method: Compositional matrix adjust. Identities = 170/411 (41%), Positives = 252/411 (61%), Gaps = 4/411 (0%) Query: 15 PQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRP 74 P+ R A+ ++ +GNA EWFD +YG FA VIA FP+ + +LLL ATFGV F RP Sbjct: 16 PRQRHAVATAILGNAFEWFDFTLYGFFAPVIATVLFPSANPFTALLLAIATFGVGFVTRP 75 Query: 75 LGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFS 134 LG +VLG Y+DR GR+ AL LS +LM +GTA+IA P+YA G+ AP + +++QGFS Sbjct: 76 LGGIVLGIYADRRGRRPALALSALLMALGTALIAIAPTYAQAGIAAPVIAVIARLLQGFS 135 Query: 135 AGGEFGSSTAFLVEHAPH-RRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGW 193 AGGE G +TA+L E AP +R F++SW AS G++++ A+ G + ++L E L +WGW Sbjct: 136 AGGEMGGATAYLNEIAPPGKRAFYTSWIQASVGLAIVCGAMLGTLATSLLDAEALRAWGW 195 Query: 194 RVPFIFGLLIAPAGIYIRRHLDEAPEFKESSE--KTNAPLRDTFAHQKMRLLIGAGSVIM 251 R+PF G+LI P G IR LDE P F ++ + ++PL + + +LIGA VI+ Sbjct: 196 RIPFALGVLIGPIGYVIRSRLDETPAFARAATEARADSPLLEVVRRHRRAVLIGASIVIL 255 Query: 252 ATVSVYLSL-YIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMIT 310 TV Y+ L Y+ TYA+ L LP+ + FA +G+ M + +PL G L+D++G + Sbjct: 256 WTVCTYVLLFYMQTYAIHVLKLPSSAGFAGSMASGVTMMLTAPLFGWLADRLGYQRVLSG 315 Query: 311 SSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGM 370 ++ +VL YP++ +L ++P L+ +Q V G+L Y +P AD+FP TG+ Sbjct: 316 AALAILVLAYPLYAYLNHAPAAGTLIGVQIVFGLLSAAYTGPIPVAFADLFPTKVLSTGL 375 Query: 371 SLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSAR 421 S AYN+ V + GGFA +TWL L+ +V+ A++SLI +L R Sbjct: 376 STAYNLTVMLIGGFAPFYLTWLSHAFDPMLAPVLYVMVAALVSLIGTLWLR 426 >RSc3060 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 433 Score = 296 bits (759), Expect = 5e-80, Method: Compositional matrix adjust. Identities = 173/407 (42%), Positives = 244/407 (59%), Gaps = 9/407 (2%) Query: 19 RAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRPLGAV 78 RAI GN LE FD ++YG +A IA FFP G+ +SL+L+ TFG F MRPLGA+ Sbjct: 12 RAIFRVVSGNFLEMFDFMVYGYYATAIAATFFPAGNAFLSLMLSLMTFGAGFLMRPLGAI 71 Query: 79 VLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGE 138 VLGAY DR GR+A L+L+++LM GT +IA +P Y +IGL AP + +G+++QGFSAGGE Sbjct: 72 VLGAYIDRHGRRAGLILTLVLMACGTMLIALVPGYGTIGLAAPLLVLIGRLLQGFSAGGE 131 Query: 139 FGSSTAFLVEHA-PHRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGWRVPF 197 G + +L E A P R+GF+ SWQ SQ ++++ AAL G +L+ L+P+++ WGWRVPF Sbjct: 132 PGGVSVYLSEIATPGRKGFYVSWQSGSQQVAVMFAALLGVLLSFKLSPKEMGEWGWRVPF 191 Query: 198 IFGLLIAPAGIYIRRHLDEAPEFKESSEKTN--APLRDTFAHQKMRLLIGAGSV--IMAT 253 + G LI P +IRR L E EF++ + LR + + L GAG++ +M T Sbjct: 192 LIGCLIVPFLFFIRRSLRETEEFEQRKHRPTMGELLRSMGENWR---LAGAGTLLAVMTT 248 Query: 254 VSVYL-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSS 312 VS YL + Y PT+ L + + S G I P++G+LSD+IGR +I S Sbjct: 249 VSFYLITAYTPTFGRNVLHMSDFDSLIVTLCVGASNLIWLPVMGSLSDRIGRRTPLIVFS 308 Query: 313 ALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSL 372 AL ++ YP +L P F +LL ++ + L Y AM L +I P R TG SL Sbjct: 309 ALTLLTAYPAMTWLIAEPSFGRLLAVELWLSFLYGGYNGAMIVTLTEIMPPQLRTTGFSL 368 Query: 373 AYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLS 419 AY++A IFGGF I T LI TG+K ++ F A+ L+A+L+ Sbjct: 369 AYSLATAIFGGFTPAIATGLIHATGNKAMPGLWLSFAAICGLVATLT 415 >PA5476 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 429 Score = 286 bits (731), Expect = 8e-77, Method: Compositional matrix adjust. Identities = 163/383 (42%), Positives = 229/383 (59%), Gaps = 3/383 (0%) Query: 20 AIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRPLGAVV 79 AI GN LE +D ++YG +A IAK FFP + SL+L+ ATFG F MRPLGAV Sbjct: 10 AIFRVVSGNFLEMYDFMVYGFYATAIAKTFFPGDNPFASLMLSLATFGAGFLMRPLGAVF 69 Query: 80 LGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGEF 139 LGAY DR GR+ L++++ LM +GT +IAF+P YA++G+ AP + G+++QGFSAG E Sbjct: 70 LGAYIDRHGRRQGLIITLGLMAMGTLLIAFVPGYATLGVAAPLLVLFGRLLQGFSAGVEL 129 Query: 140 GSSTAFLVEHA-PHRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGWRVPFI 198 G + +L E A P R+GFF SWQ ASQ ++++ A L G +LN L+P+ + WGWRVPF Sbjct: 130 GGVSVYLSEIATPGRKGFFVSWQSASQQVAVVFAGLLGVLLNQWLSPQDMGEWGWRVPFF 189 Query: 199 FGLLIAPAGIYIRRHLDEAPEFKESSEKTN-APLRDTFAHQKMRLLIGAGSVIMATVSVY 257 G LI PA IRR L+E PEF+ + + + + + + +L G V+M TVS Y Sbjct: 190 IGCLIVPALFVIRRSLEETPEFEARTHRPSLSQVLRSIGQNFGVVLAGTAMVVMTTVSFY 249 Query: 258 L-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSSALYI 316 L + Y PT+ +L L S GL FI P++GA SD+IGR P ++ +SAL + Sbjct: 250 LITAYTPTFGKNELQLSDLDSLLVTMCIGLSNFIWLPVMGAFSDRIGRKPLLLGASALAL 309 Query: 317 VLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSLAYNI 376 + YP +L P F LL ++ + L + Y AM L ++ P R TG SLAY++ Sbjct: 310 LTAYPALSWLVREPSFAGLLGVELWLSFLYSSYNGAMVVALTEVMPADVRTTGFSLAYSL 369 Query: 377 AVTIFGGFAGLIITWLIDFTGDK 399 A FGGF + TWLI ++ Sbjct: 370 ATATFGGFTPAVCTWLIHTLDNR 392