BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0084.p (297 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3569 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relate... 326 4e-89 PA0743 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relate... 297 2e-80 AGl1448 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 261 1e-69 RSp0649 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 261 2e-69 mlr1203 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 235 1e-61 >PA3569 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Pae Pseudomonas aeruginosa Length = 298 Score = 326 bits (835), Expect = 4e-89, Method: Compositional matrix adjust. Identities = 172/288 (59%), Positives = 208/288 (72%), Gaps = 2/288 (0%) Query: 4 NIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKADVVI 63 +I F+GLGNMG PMA NL+KAG V+ DL +V LVE G + ++ A+VVI Sbjct: 3 DIAFLGLGNMGGPMAANLLKAGHRVNVFDLQPKAVLGLVEQGAQGADSALQCCEGAEVVI 62 Query: 64 TMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVS 123 +MLPA +HVESLYLG+ G+LA LLIDCSTIAPE ARKVA AA A+G +LDAPVS Sbjct: 63 SMLPAGQHVESLYLGDDGLLARVAGKPLLIDCSTIAPETARKVAEAAAAKGLTLLDAPVS 122 Query: 124 GGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLGILM 183 GG GA AGTL+F+VGG E F A+P L+ MG+ I+HAG+ G+GQ K+CNNMLLGILM Sbjct: 123 GGVGGARAGTLSFIVGGPAEGFARARPVLENMGRNIFHAGDHGAGQVAKICNNMLLGILM 182 Query: 184 IGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMDTAPASKGYAGGFGVD 243 GT+EA+ LG+ NG+DP VLSE+M +SSG NW L +YNP PGVM APAS GYAGGF V Sbjct: 183 AGTAEALALGVKNGLDPAVLSEVMKQSSGGNWALNLYNPWPGVMPQAPASNGYAGGFQVR 242 Query: 244 LMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGK--LDFSSI 289 LM KDLGLA+ NA A + PLGA ARNL+ +H+ A LDFSSI Sbjct: 243 LMNKDLGLALANAQAVQASTPLGALARNLFSLHAQADAEHEGLDFSSI 290 >PA0743 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Pae Pseudomonas aeruginosa Length = 298 Score = 297 bits (761), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 168/294 (57%), Positives = 212/294 (72%) Query: 4 NIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKADVVI 63 I FIGLG+MG PMA NL+KAG+ ++ DLV+++VD LV G + AV ADVVI Sbjct: 3 QIAFIGLGHMGAPMATNLLKAGYLLNVFDLVQSAVDGLVAAGASAARSARDAVQGADVVI 62 Query: 64 TMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVS 123 +MLPAS+HVE LYL + G+LA PGTL+++CSTIAP +ARK+ AAA+ RG MLDAPVS Sbjct: 63 SMLPASQHVEGLYLDDDGLLAHIAPGTLVLECSTIAPTSARKIHAAARERGLAMLDAPVS 122 Query: 124 GGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLGILM 183 GGT GA AGTLTFMVGG EA E A+P + MG+ I+HAG G+GQ KVCNN LL +LM Sbjct: 123 GGTAGAAAGTLTFMVGGDAEALEKARPLFEAMGRNIFHAGPDGAGQVAKVCNNQLLAVLM 182 Query: 184 IGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMDTAPASKGYAGGFGVD 243 IGT+EA+ LG+ANG++ KVL+EIM +SSG NW LEVYNP PGVM+ APAS+ Y+GGF Sbjct: 183 IGTAEAMALGVANGLEAKVLAEIMRRSSGGNWALEVYNPWPGVMENAPASRDYSGGFMAQ 242 Query: 244 LMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNMLGKAQ 297 LM KDLGLA E A A+ + P+G+ A +LY + G + DFS + + Q Sbjct: 243 LMAKDLGLAQEAAQASASSTPMGSLALSLYRLLLKQGYAERDFSVVQKLFDPTQ 296 >AGl1448 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 304 Score = 261 bits (668), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 145/290 (50%), Positives = 180/290 (62%), Gaps = 3/290 (1%) Query: 4 NIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKADVVI 63 + F+GLG+MGLPMA NLVKAG++V G DL +A E G AD+ A ADVVI Sbjct: 13 TVAFLGLGHMGLPMAANLVKAGYAVRGFDLSEACRTAAREAGIPVAADATEATHAADVVI 72 Query: 64 TMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVS 123 TMLP HV S+Y ++ GTLLIDCST+ E+A K A + G +DAPVS Sbjct: 73 TMLPKGEHVVSVY---GDLVRKVSAGTLLIDCSTVDMESALKAHELAASAGCPSVDAPVS 129 Query: 124 GGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLGILM 183 GGT+GA AGTLTFM GG +AF AA+P L+ MGK I H G+ G+GQ K+CNNM+LGI M Sbjct: 130 GGTSGAAAGTLTFMCGGEPDAFSAARPVLEAMGKKIVHCGKGGNGQAAKICNNMILGISM 189 Query: 184 IGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMDTAPASKGYAGGFGVD 243 I SEA LG G+ + L ++ S SSG+ W+L Y P PG + T+PA+K Y GF Sbjct: 190 IAVSEAFVLGEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANKDYQPGFAAA 249 Query: 244 LMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNML 293 LMLKDL L+ A T + PLGA A LY+ G G DFS+I ML Sbjct: 250 LMLKDLKLSQAAANGTGVSTPLGAAASALYESFVGEGGGGRDFSAIVEML 299 >RSp0649 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Rso Ralstonia solanacearum Length = 298 Score = 261 bits (666), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 153/289 (52%), Positives = 195/289 (67%) Query: 4 NIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKADVVI 63 I FIGLGNMG PMA+NL+KAG ++S DL +V LV+ G AA AD I Sbjct: 2 KIAFIGLGNMGAPMARNLLKAGHALSVFDLDARAVQSLVDAGASAAPSPKAAATGADCAI 61 Query: 64 TMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVS 123 TMLPA+ HV S+ + G+LA G +ID STI P++A+ +AA A A G +DAPVS Sbjct: 62 TMLPAAAHVHSVLTADDGILAGIAKGVPIIDSSTIDPDSAKALAALATAHGNPFVDAPVS 121 Query: 124 GGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLGILM 183 GGT GA AGTLTFMVGG FE +P L MGK + H GE+G+GQ K+CNN++LGI M Sbjct: 122 GGTGGAAAGTLTFMVGGGAAVFEQVRPVLSAMGKNLVHCGEAGAGQGAKICNNLVLGITM 181 Query: 184 IGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMDTAPASKGYAGGFGVD 243 G +EA+ LG A G+DPKVL I++ S+GR W+ + YNP PGV++TAP+S+GY GGFG D Sbjct: 182 AGVAEAMSLGEALGIDPKVLGGIINTSTGRCWSSDTYNPFPGVIETAPSSRGYTGGFGTD 241 Query: 244 LMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNM 292 LMLKDLGLA + A + + LGA A+ LY S GAGKLDFS++ + Sbjct: 242 LMLKDLGLAGDAAKSVRQPVYLGALAQQLYQASSSQGAGKLDFSAVIRL 290 >mlr1203 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Mlo Mesorhizobium loti Length = 294 Score = 235 bits (599), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 146/289 (50%), Positives = 177/289 (61%), Gaps = 3/289 (1%) Query: 5 IVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKADVVIT 64 I FIGLGNMG PMA NLVKAG V G DL+ ++ E G A++ AAV ADVVIT Sbjct: 4 IAFIGLGNMGNPMAANLVKAGHQVHGFDLMPENLTVAREHGVVVMANAPAAVKDADVVIT 63 Query: 65 MLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVSG 124 MLPA +HV S+Y + AK L ID STI E+ARK A A G +DAPVSG Sbjct: 64 MLPAGKHVLSVY---EDIAPKAKKDALFIDSSTIDVESARKAHAIAAKHGLLSIDAPVSG 120 Query: 125 GTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLGILMI 184 GT GA AGTLTFM GGS +AF AA+P L+ M I H G G+GQ K+CNNM+LGI MI Sbjct: 121 GTGGAAAGTLTFMAGGSNDAFAAAEPVLKPMAGRIVHCGGDGAGQAAKICNNMILGISMI 180 Query: 185 GTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMDTAPASKGYAGGFGVDL 244 G +EA L G+ + L ++ S SSG+ W+L Y P PG + T+PA++ Y GF L Sbjct: 181 GVAEAFVLAEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANRDYRPGFAAAL 240 Query: 245 MLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNML 293 MLKDL L+ E A + PLGA A LY + + G G DFS I N L Sbjct: 241 MLKDLKLSQEAAQSAGAVTPLGAEAAQLYALFNAQGHGGADFSGIINFL 289