BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0006.p (446 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0312 [V] COG0841 Cation/multidrug efflux pump Rso Ralstonia s... 615 e-176 AGl3217 [V] COG0841 Cation/multidrug efflux pump Atu Agrobacteri... 482 e-136 SMc02867 [V] COG0841 Cation/multidrug efflux pump Sme Sinorhizob... 479 e-135 RSc0010 [V] COG0841 Cation/multidrug efflux pump Rso Ralstonia s... 468 e-131 PA4598 [V] COG0841 Cation/multidrug efflux pump Pae Pseudomonas ... 468 e-131 >RSp0312 [V] COG0841 Cation/multidrug efflux pump Rso Ralstonia solanacearum Length = 1049 Score = 615 bits (1587), Expect = e-176, Method: Compositional matrix adjust. Identities = 306/403 (75%), Positives = 359/403 (89%) Query: 1 MPRFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDG 60 MP+FFI RP+FAWV+ALFI+L G IA+ +LPIARYPSVAPPTV+++ATYPGATPQTMNDG Sbjct: 1 MPQFFIRRPVFAWVIALFIILLGAIAIPKLPIARYPSVAPPTVNLSATYPGATPQTMNDG 60 Query: 61 VISLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQ 120 VISLIERELS VKNLLYFESS+DTSG+A I TFKPGTDP +AQVDVQNKLKAIE RLPQ Sbjct: 61 VISLIERELSSVKNLLYFESSSDTSGSASIVATFKPGTDPAMAQVDVQNKLKAIEPRLPQ 120 Query: 121 TVRQNGVTVESAASSFLLMVSLTSVDGRYDEVRLNDYLARNIVEELRRMEGVGRVQLFGA 180 VRQNG+TVESA+S FL+++ L S DGR+DE L+DY+ARN+VEELRR+EGVGRVQLFGA Sbjct: 121 VVRQNGLTVESASSGFLMIIGLQSDDGRFDETALSDYMARNVVEELRRIEGVGRVQLFGA 180 Query: 181 EQAMRIWIDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQGQL 240 EQAMRIW+DP KL AYGL++ D+S AISQQNAQIAPG +G +PA+ Q++ +PLTVQGQL Sbjct: 181 EQAMRIWVDPAKLIAYGLSMNDLSAAISQQNAQIAPGSVGASPALPAQRVTVPLTVQGQL 240 Query: 241 QSPAQFAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQLSPGANAV 300 +P QFAAIVL+A A+GS+V++GDVARVELGAQSY F NRENGK A AAVQL+PGANAV Sbjct: 241 TTPEQFAAIVLRANANGSKVVMGDVARVELGAQSYGFINRENGKAATAAAVQLAPGANAV 300 Query: 301 RTAAAIQARMEELRPSLPQGMAFSLPYNTAPFVKISIQKVVQTLIEAMVLVFLVMYLFLQ 360 RTA +QARM EL ++P GM++S+P+NTAPFVKISI+KV+QTLIEAMVLVFLVMYLFLQ Sbjct: 301 RTAEGVQARMAELSRTMPAGMSYSIPFNTAPFVKISIEKVIQTLIEAMVLVFLVMYLFLQ 360 Query: 361 NVRYTVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIG 403 NVRYT+IPAIVAPIALLGT +M +G+S+NVLTMFGMVLAIG Sbjct: 361 NVRYTLIPAIVAPIALLGTFTIMLASGFSINVLTMFGMVLAIG 403 >AGl3217 [V] COG0841 Cation/multidrug efflux pump Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 1044 Score = 482 bits (1241), Expect = e-136, Method: Compositional matrix adjust. Identities = 231/405 (57%), Positives = 311/405 (76%) Query: 1 MPRFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDG 60 M RFFIDRPIFAWVVA+FI+L G++A+ LPIA+YP+VAPP +SI YPGA+P+ + Sbjct: 1 MARFFIDRPIFAWVVAIFIMLAGLLAIPMLPIAQYPNVAPPQISIGTNYPGASPEDIYQS 60 Query: 61 VISLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQ 120 V IE EL+GV L+YFES++++SG I+VTF+PGT+ AQV+VQN++ +E RLP+ Sbjct: 61 VTRPIEEELNGVPGLIYFESTSESSGRISINVTFEPGTNIGEAQVEVQNRIARVEPRLPR 120 Query: 121 TVRQNGVTVESAASSFLLMVSLTSVDGRYDEVRLNDYLARNIVEELRRMEGVGRVQLFGA 180 +V Q G+ VE A +SFL+MV+LTSVDG D + L DYL+RN++ ELRR+ GVG QLF Sbjct: 121 SVTQQGLRVEQAGTSFLMMVALTSVDGNTDAIGLGDYLSRNVLGELRRVPGVGSAQLFAT 180 Query: 181 EQAMRIWIDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQGQL 240 +++MRIW+DP K+ LT D+ AI QN+Q+A GR+G +P GQQI+ + VQGQL Sbjct: 181 QRSMRIWMDPDKMLGLSLTSDDVINAIQAQNSQVAAGRIGASPNPVGQQISATVNVQGQL 240 Query: 241 QSPAQFAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQLSPGANAV 300 SP +F +IVL+A DGS V L DVARVE+G +SY F++R NGKP+ VQLSP ANA+ Sbjct: 241 TSPEEFGSIVLRANPDGSSVRLRDVARVEVGGESYNFSSRLNGKPSAAIGVQLSPTANAM 300 Query: 301 RTAAAIQARMEELRPSLPQGMAFSLPYNTAPFVKISIQKVVQTLIEAMVLVFLVMYLFLQ 360 +T+ ++A MEEL PQG+ + +PY+T+PFVKISI+KV+ TLIEAM+LVF+VM++FLQ Sbjct: 301 QTSEGVRATMEELSRYFPQGIEYEIPYDTSPFVKISIEKVIHTLIEAMILVFVVMFVFLQ 360 Query: 361 NVRYTVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGXM 405 N+RYT+IP +V P+ALLGT AVM ++G+S+NVLTMF MVLAIG + Sbjct: 361 NIRYTIIPTLVVPVALLGTCAVMYVSGFSINVLTMFAMVLAIGIL 405 >SMc02867 [V] COG0841 Cation/multidrug efflux pump Sme Sinorhizobium meliloti Length = 1048 Score = 479 bits (1234), Expect = e-135, Method: Compositional matrix adjust. Identities = 233/405 (57%), Positives = 304/405 (75%) Query: 1 MPRFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDG 60 MP FFIDRPIFAWVVA+FI++ G+IA+ LP+++YP VAPP +SIN YPGA+ Q Sbjct: 1 MPSFFIDRPIFAWVVAIFIMIAGIIAIPLLPVSQYPDVAPPQISINTNYPGASSQDTYQS 60 Query: 61 VISLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQ 120 V LIE EL+GV+ LLYFESS +SG+ I TF+PGTDP A VD+QN+++ +E RLP Sbjct: 61 VTRLIEDELNGVEGLLYFESSTSSSGSVSIDATFQPGTDPSQASVDIQNRVQRVEPRLPD 120 Query: 121 TVRQNGVTVESAASSFLLMVSLTSVDGRYDEVRLNDYLARNIVEELRRMEGVGRVQLFGA 180 VRQ GV V+ A + FLL++SLTS DG D + L DYL+RN++ E++R+ GVGR QLF Sbjct: 121 PVRQQGVQVDEAGAGFLLIISLTSTDGSMDAIGLGDYLSRNVLSEIQRVPGVGRAQLFAT 180 Query: 181 EQAMRIWIDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQGQL 240 E++MR+W+DP K+ LT D++ AI QNAQIA G +G P QQ+ P+ ++GQL Sbjct: 181 ERSMRVWLDPDKMLGLNLTAADVTAAIQAQNAQIASGSIGAQPNPITQQVTAPVVIKGQL 240 Query: 241 QSPAQFAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQLSPGANAV 300 SP +F AIVL+A ADGS V L DVAR+E+G +SY F+ R NG P+ AVQLSP NA+ Sbjct: 241 SSPEEFGAIVLRANADGSAVRLRDVARLEIGGESYTFSTRLNGSPSAAIAVQLSPSGNAM 300 Query: 301 RTAAAIQARMEELRPSLPQGMAFSLPYNTAPFVKISIQKVVQTLIEAMVLVFLVMYLFLQ 360 T+AAI+ARM+EL P+G+ +S+PY+T+PFV +SI+KV+ TL+EA+ LVFLVM+LFLQ Sbjct: 301 STSAAIKARMDELAEFFPEGLEYSIPYDTSPFVAVSIEKVLHTLVEAVGLVFLVMFLFLQ 360 Query: 361 NVRYTVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGXM 405 NVRYT+IP IV P+ALLGT AVM G+S+NVLTMFGMVLAIG + Sbjct: 361 NVRYTIIPTIVVPVALLGTCAVMLAMGFSINVLTMFGMVLAIGIL 405 >RSc0010 [V] COG0841 Cation/multidrug efflux pump Rso Ralstonia solanacearum Length = 1049 Score = 468 bits (1205), Expect = e-131, Method: Compositional matrix adjust. Identities = 227/406 (55%), Positives = 305/406 (75%), Gaps = 1/406 (0%) Query: 1 MPRFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDG 60 M +FFIDRP+FAWV+ALFI++ G I+++QLPIA+YP++APP++ I ATYPGA+ +T++D Sbjct: 1 MAKFFIDRPVFAWVLALFIIVAGAISITQLPIAQYPTIAPPSIIITATYPGASAKTLDDA 60 Query: 61 VISLIERELSGVKNLLYFES-SADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLP 119 V S+IE+E++G LLY ES S +G A I+VTFKPGTDP LAQVDVQN+LK +EARLP Sbjct: 61 VTSIIEQEMNGADGLLYIESVSQAGNGQATITVTFKPGTDPALAQVDVQNRLKRVEARLP 120 Query: 120 QTVRQNGVTVESAASSFLLMVSLTSVDGRYDEVRLNDYLARNIVEELRRMEGVGRVQLFG 179 +V Q GV V+ S+FLL +L S DG+ D V L DY++RN++ E++R+ GVG+ LFG Sbjct: 121 SSVTQQGVQVDKTRSNFLLFATLISKDGKMDPVALGDYISRNVLNEVKRVPGVGQAVLFG 180 Query: 180 AEQAMRIWIDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQGQ 239 E+AMRIWIDP KL Y LT TD+ AI QNA ++ G LGD P+ Q IA + V+GQ Sbjct: 181 TERAMRIWIDPAKLVGYKLTPTDVYNAIRNQNALVSAGTLGDLPSTSDQPIAATVVVEGQ 240 Query: 240 LQSPAQFAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQLSPGANA 299 + + QF IVL +K DGS+V + DVAR+ELG Q+YA + R NG+P VQLSP NA Sbjct: 241 MTTTEQFGNIVLVSKPDGSQVRIKDVARLELGGQTYATSARINGQPISAIGVQLSPTGNA 300 Query: 300 VRTAAAIQARMEELRPSLPQGMAFSLPYNTAPFVKISIQKVVQTLIEAMVLVFLVMYLFL 359 + TA A++A+++EL P G+ + +PY+T+ FV+ISI++VV+TL EAM LVFLVM +FL Sbjct: 301 LGTAKAVKAKLDELSKYFPAGVEYKIPYDTSKFVQISIEEVVKTLFEAMALVFLVMLVFL 360 Query: 360 QNVRYTVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGXM 405 QN+RYT+IP+IV PI+LLG A M G+S+NVLTMFG+VLAIG + Sbjct: 361 QNIRYTLIPSIVVPISLLGAFATMNALGFSINVLTMFGLVLAIGIL 406 >PA4598 [V] COG0841 Cation/multidrug efflux pump Pae Pseudomonas aeruginosa Length = 1043 Score = 468 bits (1203), Expect = e-131, Method: Compositional matrix adjust. Identities = 226/407 (55%), Positives = 300/407 (73%), Gaps = 2/407 (0%) Query: 1 MPRFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDG 60 M FFI RP FAWVVALFI L G++ +S+LP+A+YP+VAPP ++I ATYPGA+ + + D Sbjct: 1 MSEFFIKRPNFAWVVALFISLAGLLVISKLPVAQYPNVAPPQITITATYPGASAKVLVDS 60 Query: 61 VISLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQ 120 V S++E L+G K LLYFES+ +++GTA+I VTF+PGTDP+LAQVDVQN+LK EAR+PQ Sbjct: 61 VTSVLEESLNGAKGLLYFESTNNSNGTAEIVVTFEPGTDPDLAQVDVQNRLKKAEARMPQ 120 Query: 121 TVRQNGVTVESAASSFLLMVSLTSVDG--RYDEVRLNDYLARNIVEELRRMEGVGRVQLF 178 V G+ VE ++ FLL+ +L+ +G R D L DY ARNI ELRR+ GVG++Q F Sbjct: 121 AVLTQGLQVEQTSAGFLLIYALSYKEGAQRSDTTALGDYAARNINNELRRLPGVGKLQFF 180 Query: 179 GAEQAMRIWIDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQG 238 +E AMR+WIDP KL +GL++ D+S AI QN Q+ G G AP Q++ L V+G Sbjct: 181 SSEAAMRVWIDPQKLVGFGLSIDDVSNAIRGQNVQVPAGAFGSAPGSSAQELTATLAVKG 240 Query: 239 QLQSPAQFAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQLSPGAN 298 L P +F +VL+A DGS V L DVAR+ELG +SY ++R NG P A+QLSPGAN Sbjct: 241 TLDDPQEFGQVVLRANEDGSLVRLADVARLELGKESYNISSRLNGTPTVGGAIQLSPGAN 300 Query: 299 AVRTAAAIQARMEELRPSLPQGMAFSLPYNTAPFVKISIQKVVQTLIEAMVLVFLVMYLF 358 A++TA ++ R+ EL P+ M +S+PY+T+ FV ++I+KV+ TLIEAMVLVFLVM+LF Sbjct: 301 AIQTATLVKQRLAELSAFFPEDMQYSVPYDTSRFVDVAIEKVIHTLIEAMVLVFLVMFLF 360 Query: 359 LQNVRYTVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGXM 405 LQNVRYT+IP+IV P+ LLGTL VM L G+SVN++TMFGMVLAIG + Sbjct: 361 LQNVRYTLIPSIVVPVCLLGTLMVMYLLGFSVNMMTMFGMVLAIGIL 407